MGE detailed information
MGE type: Conjugative plasmid MGE length: 160021 bp
Accession: NZ_CP019954 Location: 1..160021 Organism: Escherichia coli M8 Replicon: plasmid unnamed1
Accession: NZ_CP019954 Location: 1..160021 Organism: Escherichia coli M8 Replicon: plasmid unnamed1
Gene structure
The 5 kb flanking regions of this MGE are displayed.
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| B2H83_RS25320 () | 93..914 | + | 822 | WP_129542110.1 | F-type conjugal transfer pilus assembly protein TraB | - |
| B2H83_RS25325 () | 904..1464 | + | 561 | WP_061892134.1 | conjugal transfer protein TraP | - |
| B2H83_RS25330 () | 1403..1675 | + | 273 | WP_061892133.1 | conjugal transfer protein TrbD | - |
| B2H83_RS25335 () | 1672..2187 | + | 516 | WP_061892132.1 | type IV conjugative transfer system lipoprotein TraV | - |
| B2H83_RS25340 () | 2322..2543 | + | 222 | WP_021532666.1 | conjugal transfer protein TraR | - |
| B2H83_RS25345 () | 2536..3009 | + | 474 | WP_061892131.1 | hypothetical protein | - |
| B2H83_RS25350 () | 3089..3307 | + | 219 | WP_061892130.1 | hypothetical protein | - |
| B2H83_RS25355 () | 3335..3682 | + | 348 | WP_000802783.1 | hypothetical protein | - |
| B2H83_RS25360 () | 3809..6439 | + | 2631 | WP_129542111.1 | type IV secretion system protein TraC | - |
| B2H83_RS25365 () | 6436..6822 | + | 387 | WP_129542112.1 | type-F conjugative transfer system protein TrbI | - |
| B2H83_RS25370 () | 6819..7451 | + | 633 | WP_129542113.1 | type-F conjugative transfer system protein TraW | - |
| B2H83_RS25375 () | 7448..8440 | + | 993 | WP_129542114.1 | conjugal transfer pilus assembly protein TraU | - |
| B2H83_RS25380 () | 8461..8640 | + | 180 | WP_100007973.1 | hypothetical protein | - |
| B2H83_RS25385 () | 8696..9007 | + | 312 | WP_129542162.1 | hypothetical protein | - |
| B2H83_RS25390 () | 9070..9591 | + | 522 | WP_129542115.1 | hypothetical protein | - |
| B2H83_RS25395 () | 9618..10256 | + | 639 | WP_129542116.1 | type-F conjugative transfer system pilin assembly protein TrbC | - |
| B2H83_RS25400 () | 10253..10624 | + | 372 | WP_129542117.1 | hypothetical protein | - |
| B2H83_RS25405 () | 10650..11072 | + | 423 | WP_001229309.1 | HNH endonuclease | - |
| B2H83_RS25410 () | 11069..12898 | + | 1830 | WP_129542118.1 | type-F conjugative transfer system mating-pair stabilization protein TraN | - |
| B2H83_RS25415 () | 12925..13113 | + | 189 | WP_129542163.1 | conjugal transfer protein TrbE | - |
| B2H83_RS25420 () | 13175..13918 | + | 744 | WP_129542119.1 | type-F conjugative transfer system pilin assembly protein TraF | - |
| B2H83_RS25425 () | 13932..14294 | + | 363 | WP_129542120.1 | hypothetical protein | - |
| B2H83_RS25430 () | 14380..14826 | + | 447 | WP_129542121.1 | hypothetical protein | - |
| B2H83_RS25435 () | 14893..15174 | + | 282 | WP_129542122.1 | type-F conjugative transfer system pilin chaperone TraQ | - |
| B2H83_RS25440 () | 15161..15706 | + | 546 | WP_129542123.1 | type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB | - |
| B2H83_RS25445 () | 15636..15983 | + | 348 | WP_126697346.1 | P-type conjugative transfer protein TrbJ | - |
| B2H83_RS25450 () | 15931..16356 | + | 426 | WP_129542124.1 | F-type conjugal transfer protein TrbF | - |
| B2H83_RS25455 () | 16343..17716 | + | 1374 | WP_129542125.1 | conjugal transfer protein TraH | - |
| B2H83_RS25460 () | 17713..20550 | + | 2838 | WP_129542126.1 | conjugal transfer mating pair stabilization protein TraG | - |
| B2H83_RS25465 () | 20583..21086 | + | 504 | WP_129542127.1 | conjugal transfer entry exclusion protein TraS | - |
| B2H83_RS25470 () | 21126..21857 | + | 732 | WP_061892709.1 | complement resistance protein TraT | - |
| B2H83_RS25475 () | 22060..22800 | + | 741 | WP_061892706.1 | hypothetical protein | - |
| B2H83_RS25480 () | 22851..25031 | + | 2181 | WP_129542128.1 | type IV conjugative transfer system coupling protein TraD | - |
| B2H83_RS25485 () | 25031..30301 | + | 5271 | WP_061892704.1 | Multifunctional conjugation protein TraI | Relaxase |
| B2H83_RS25490 () | 30321..31061 | + | 741 | WP_032334797.1 | conjugal transfer pilus acetylase TraX | - |
| B2H83_RS25495 () | 31116..31676 | + | 561 | WP_061892703.1 | fertility inhibition protein FinO | - |
| B2H83_RS25500 () | 31812..32024 | + | 213 | WP_061892702.1 | hypothetical protein | - |
| B2H83_RS25505 () | 32270..32730 | + | 461 | Protein_37 | thermonuclease family protein | - |
| B2H83_RS25510 () | 32776..32985 | + | 210 | WP_061892701.1 | hemolysin expression modulator Hha | - |
| B2H83_RS25515 () | 33023..33613 | + | 591 | WP_061892700.1 | DUF2726 domain-containing protein | - |
| B2H83_RS25520 () | 33853..34107 | + | 255 | WP_000083830.1 | replication regulatory protein RepA | - |
| B2H83_RS25530 () | 34345..34419 | + | 75 | WP_061892708.1 | RepA leader peptide Tap | - |
| B2H83_RS25535 () | 34412..35269 | + | 858 | WP_061892699.1 | incFII family plasmid replication initiator RepA | - |
| B2H83_RS26555 () | 35968..36126 | + | 159 | WP_061892698.1 | hypothetical protein | - |
| B2H83_RS25545 () | 36206..36859 | + | 654 | WP_129542129.1 | CPBP family intramembrane metalloprotease | - |
| B2H83_RS25550 () | 36952..37209 | + | 258 | WP_061892696.1 | type II toxin-antitoxin system antitoxin PemI | - |
| B2H83_RS25555 () | 37142..37543 | + | 402 | WP_001398199.1 | type II toxin-antitoxin system toxin endoribonuclease PemK | - |
| B2H83_RS25570 () | 37931..38227 | - | 297 | WP_024133129.1 | hypothetical protein | - |
| B2H83_RS25580 () | 39339..40568 | + | 1230 | WP_024227651.1 | autotransporter strand-loop-strand O-heptosyltransferase | - |
| B2H83_RS25585 () | 40552..43461 | + | 2910 | WP_129542130.1 | putative autotransporter | VF |
| B2H83_RS25590 () | 43572..43868 | + | 297 | WP_129542131.1 | Cyclic di-GMP phosphodiesterase PdeL | VF |
| B2H83_RS25595 () | 44084..44783 | - | 700 | Protein_51 | AraC family transcriptional regulator | - |
| B2H83_RS25600 () | 45036..45300 | + | 265 | Protein_52 | IS256 family transposase IS1414 | Transposase |
| B2H83_RS25605 () | 45891..46163 | + | 273 | WP_073972144.1 | Major pilu subunit operon regulatory protein PapB | VF |
| B2H83_RS25610 () | 46291..46809 | + | 519 | WP_032334824.1 | hypothetical protein | VF |
| B2H83_RS25615 () | 46894..49299 | + | 2406 | WP_077875802.1 | Outer membrane usher protein FimD | VF |
| B2H83_RS25620 () | 49292..49984 | + | 693 | WP_024227327.1 | Chaperone protein FaeE | VF |
| B2H83_RS25625 () | 49971..50489 | + | 519 | WP_061892257.1 | fimbrial protein | - |
| B2H83_RS25630 () | 50497..51066 | + | 570 | WP_032334819.1 | hypothetical protein | - |
| B2H83_RS25635 () | 51191..52150 | + | 960 | WP_061892258.1 | hypothetical protein | - |
| B2H83_RS25640 () | 52314..53090 | + | 777 | WP_129542132.1 | EAL domain-containing protein | - |
| B2H83_RS25645 () | 53341..53934 | + | 594 | WP_032334815.1 | histidine phosphatase family protein | - |
| B2H83_RS25650 () | 53957..54121 | - | 165 | Protein_62 | IS1 family transposase | - |
| B2H83_RS26645 () | 54245..54415 | - | 171 | Protein_63 | adhesin | - |
| B2H83_RS25660 () | 54412..54957 | + | 546 | Protein_64 | IS21 family transposase ISEc12 | Transposase |
| B2H83_RS25665 () | 54972..55718 | + | 747 | WP_129542164.1 | IS21 family transposase ISEc12 | Transposase |
| B2H83_RS25670 () | 55884..57078 | - | 1195 | Protein_66 | IS91 family transposase IS91 | Transposase |
| B2H83_RS25675 () | 57078..57443 | - | 366 | WP_061892118.1 | hypothetical protein | - |
| B2H83_RS25685 () | 60229..61668 | + | 1440 | WP_164997246.1 | MFS transporter | - |
| B2H83_RS25690 () | 61729..63408 | + | 1680 | WP_061892152.1 | glycoside hydrolase family 43 protein | - |
| B2H83_RS25695 () | 63916..64098 | + | 183 | Protein_71 | IS3 family transposase ISEc17 | Transposase |
| B2H83_RS25705 () | 64176..64448 | + | 273 | WP_011310366.1 | hypothetical protein | - |
| B2H83_RS25710 () | 64429..65634 | + | 1206 | Protein_73 | IS91 family transposase ISSbo1 | Transposase |
| B2H83_RS25715 () | 65900..66151 | + | 252 | WP_001132895.1 | type II toxin-antitoxin system Phd/YefM family antitoxin | Antitoxin Detail |
| B2H83_RS25720 () | 66148..66435 | + | 288 | WP_001270417.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin Detail |
| B2H83_RS25725 () | 66434..66715 | + | 282 | Protein_76 | IS3 family transposase ISKpn37 | Transposase |
| B2H83_RS25735 () | 67350..68327 | + | 978 | WP_061892150.1 | RepB family plasmid replication initiator protein | - |
| B2H83_RS25740 () | 68606..69346 | - | 741 | WP_001066941.1 | tyrosine-type recombinase/integrase | - |
| B2H83_RS25750 () | 70205..71047 | - | 843 | WP_077698982.1 | AraC family transcriptional regulator | - |
| B2H83_RS25755 () | 71458..72027 | + | 570 | WP_061892148.1 | type 1 fimbrial protein | - |
| B2H83_RS25760 () | 72102..72812 | + | 711 | WP_032334886.1 | putative fimbrial chaperone protein ElfD | VF |
| B2H83_RS25765 () | 72778..75363 | + | 2586 | WP_164997245.1 | Outer membrane usher protein FimD | VF |
| B2H83_RS25770 () | 75380..76306 | + | 927 | WP_129542133.1 | fimbrial protein | - |
| B2H83_RS25775 () | 76390..77022 | - | 633 | Protein_84 | transposase family protein | - |
| B2H83_RS25780 () | 77127..77507 | + | 381 | WP_129542134.1 | IS66 family transposase ISEc8 | Transposase |
| B2H83_RS25785 () | 77504..77851 | + | 348 | WP_061892145.1 | IS66 family transposase ISEc8 | Transposase |
| B2H83_RS25790 () | 77901..79314 | + | 1414 | Protein_87 | IS66 family transposase ISEc8 | Transposase |
| B2H83_RS25795 () | 79507..79866 | + | 360 | Protein_88 | IS21 family transposase ISEc12 | Transposase |
| B2H83_RS25800 () | 79913..81532 | - | 1620 | WP_075262665.1 | ISL3 family transposase ISEc38 | Transposase |
| B2H83_RS25805 () | 81676..81987 | - | 312 | WP_129542165.1 | hypothetical protein | - |
| B2H83_RS25815 () | 82149..83720 | - | 1572 | WP_000381416.1 | IS66 family transposase IS679 | Transposase |
| B2H83_RS25820 () | 83740..84087 | - | 348 | WP_000624618.1 | IS66 family transposase ISCro1 | Transposase |
| B2H83_RS25825 () | 84087..84737 | - | 651 | WP_000993931.1 | IS66 family transposase IS679 | Transposase |
| B2H83_RS25830 () | 84819..85591 | - | 773 | Protein_94 | DUF1281 domain-containing protein | - |
| B2H83_RS25835 () | 85668..87392 | - | 1725 | WP_031618787.1 | group II intron reverse transcriptase/maturase | - |
| B2H83_RS25855 () | 88415..88663 | + | 249 | WP_129542135.1 | DinI-like family protein | - |
| B2H83_RS25860 () | 88660..89097 | + | 438 | WP_103745715.1 | translesion error-prone DNA polymerase V autoproteolytic subunit | - |
| B2H83_RS26650 () | 89097..90197 | + | 1101 | Protein_98 | DUF4113 domain-containing protein | - |
| B2H83_RS25875 () | 90202..90594 | - | 393 | WP_000340829.1 | plasmid partitioning/stability family protein | - |
| B2H83_RS25880 () | 90599..91570 | - | 972 | WP_001103690.1 | hypothetical protein | - |
| B2H83_RS25885 () | 91799..92443 | + | 645 | WP_000633911.1 | AAA family ATPase | - |
| B2H83_RS25890 () | 92437..92712 | + | 276 | WP_000239529.1 | hypothetical protein | - |
| B2H83_RS25895 () | 92849..93625 | - | 777 | WP_129542136.1 | site-specific integrase | - |
| B2H83_RS25900 () | 93627..94040 | - | 414 | WP_129542137.1 | hypothetical protein | - |
| B2H83_RS25910 () | 94609..94866 | + | 258 | WP_000371885.1 | hypothetical protein | - |
| B2H83_RS25915 () | 94866..95456 | + | 591 | WP_032334750.1 | hypothetical protein | - |
| B2H83_RS25920 () | 95633..97252 | + | 1620 | WP_075262665.1 | ISL3 family transposase ISEc38 | Transposase |
| B2H83_RS25925 () | 97306..97590 | - | 285 | Protein_108 | hypothetical protein | - |
| B2H83_RS25930 () | 97678..98577 | + | 900 | Protein_109 | IS110 family transposase IS5075 | Transposase |
| B2H83_RS25935 () | 99063..101315 | + | 2253 | WP_129542139.1 | Catalase HPII | VF |
| B2H83_RS25940 () | 101700..101954 | + | 255 | WP_032334811.1 | type II toxin-antitoxin system Phd/YefM family antitoxin | - |
| B2H83_RS25945 () | 101954..102373 | + | 420 | WP_032334850.1 | type II toxin-antitoxin system VapC family toxin | - |
| B2H83_RS25950 () | 102433..102882 | + | 450 | Protein_113 | protein rep | - |
| B2H83_RS25955 () | 103000..103728 | + | 729 | WP_032334813.1 | membrane lipoprotein lipid attachment site-containing protein | - |
| B2H83_RS25960 () | 104011..104757 | - | 747 | WP_129542164.1 | IS21 family transposase ISEc12 | Transposase |
| B2H83_RS25965 () | 104772..105959 | - | 1188 | Protein_116 | IS21 family transposase ISEc12 | Transposase |
| B2H83_RS25970 () | 106057..107676 | + | 1620 | WP_075262665.1 | ISL3 family transposase ISEc38 | Transposase |
| B2H83_RS25975 () | 107913..109613 | - | 1701 | WP_129542140.1 | group II intron reverse transcriptase/maturase | - |
| B2H83_RS25980 () | 110193..110846 | + | 654 | WP_129542141.1 | DNA methylase | - |
| B2H83_RS25985 () | 110847..111068 | + | 222 | WP_001104887.1 | hypothetical protein | - |
| B2H83_RS25990 () | 111080..111514 | + | 435 | WP_000274404.1 | DUF1380 family protein | - |
| B2H83_RS25995 () | 111559..111778 | + | 220 | Protein_122 | hypothetical protein | - |
| B2H83_RS26000 () | 112202..112768 | + | 567 | Protein_123 | DUF1472 domain-containing protein | - |
| B2H83_RS26005 () | 112864..113166 | + | 303 | WP_129542167.1 | antirestriction protein | - |
| B2H83_RS26010 () | 113213..113635 | + | 423 | WP_129542142.1 | DUF1380 family protein | - |
| B2H83_RS26015 () | 113632..113823 | + | 192 | WP_001027495.1 | hypothetical protein | - |
| B2H83_RS26030 () | 115245..117146 | + | 1902 | WP_129542143.1 | group II intron reverse transcriptase/maturase | - |
| B2H83_RS26035 () | 117296..117781 | + | 486 | WP_129542144.1 | single-stranded DNA-binding protein | - |
| B2H83_RS26040 () | 117838..118071 | + | 234 | WP_024261882.1 | DUF905 domain-containing protein | - |
| B2H83_RS26045 () | 118133..120091 | + | 1959 | WP_129542145.1 | ParB/RepB/Spo0J family partition protein | - |
| B2H83_RS26050 () | 120146..120580 | + | 435 | WP_023166801.1 | conjugation system SOS inhibitor PsiB | - |
| B2H83_RS26055 () | 120577..121296 | + | 720 | WP_129542146.1 | plasmid SOS inhibition protein A | - |
| B2H83_RS26060 () | 121293..121889 | + | 597 | WP_129542147.1 | hypothetical protein | - |
| B2H83_RS26065 () | 121961..122431 | + | 471 | WP_077780355.1 | YubH family protein | - |
| B2H83_RS26070 () | 122352..122852 | + | 501 | WP_000117626.1 | antirestriction protein ArdA | - |
| B2H83_RS26075 () | 122992..123291 | - | 300 | WP_089592347.1 | hypothetical protein | - |
| B2H83_RS26085 () | 123581..124015 | + | 435 | WP_000218863.1 | hypothetical protein | - |
| B2H83_RS26090 () | 124107..124373 | + | 267 | WP_001247862.1 | hypothetical protein | - |
| B2H83_RS26095 () | 124438..125379 | + | 942 | WP_129542148.1 | Recombination-promoting nuclease RpnA | VF |
| B2H83_RS26100 () | 125379..125624 | + | 246 | WP_174705345.1 | hypothetical protein | - |
| B2H83_RS26105 () | 125655..125906 | - | 252 | WP_000148285.1 | helix-turn-helix domain-containing protein | - |
| B2H83_RS26110 () | 125940..126137 | - | 198 | WP_129542150.1 | hypothetical protein | - |
| B2H83_RS26120 () | 126486..127334 | + | 849 | WP_129542151.1 | DUF4942 domain-containing protein | - |
| B2H83_RS26125 () | 127419..127754 | - | 336 | WP_000157095.1 | molybdopterin-guanine dinucleotide biosynthesis protein MobC | - |
| B2H83_RS26130 () | 127988..128320 | + | 333 | WP_001283947.1 | IncI1-type relaxosome accessory protein NikA | - |
| B2H83_RS26135 () | 128331..131027 | + | 2697 | WP_129542152.1 | Protein TraI | Relaxase |
| B2H83_RS26140 () | 131053..131625 | + | 573 | WP_129542153.1 | hypothetical protein | - |
| B2H83_RS26145 () | 131675..133864 | - | 2190 | WP_129542154.1 | peptidase domain-containing ABC transporter | - |
| B2H83_RS26150 () | 133864..135117 | - | 1254 | WP_031618765.1 | HlyD family efflux transporter periplasmic adaptor subunit | - |
| B2H83_RS26155 () | 135220..135855 | - | 636 | WP_129542155.1 | CPBP family intramembrane metalloprotease | - |
| B2H83_RS26165 () | 136640..138931 | - | 2292 | WP_129542156.1 | conjugal transfer protein TrbC | - |
| B2H83_RS26170 () | 138924..139994 | - | 1071 | WP_052974687.1 | IncI1-type conjugal transfer protein TrbB | - |
| B2H83_RS26175 () | 140013..141221 | - | 1209 | WP_129542157.1 | IncI1-type conjugal transfer protein TrbA | - |
| - () | 141401..141458 | - | 58 | NuclAT_0 | - | Antitoxin Detail |
| - () | 141401..141458 | - | 58 | NuclAT_0 | - | Antitoxin Detail |
| - () | 141401..141458 | - | 58 | NuclAT_0 | - | Antitoxin Detail |
| - () | 141401..141458 | - | 58 | NuclAT_0 | - | Antitoxin Detail |
| B2H83_RS26180 () | 141513..141665 | + | 153 | WP_001331364.1 | Hok/Gef family protein | Toxin Detail |
| B2H83_RS26185 () | 141737..141988 | - | 252 | WP_001291965.1 | hypothetical protein | - |
| B2H83_RS26190 () | 142288..142584 | + | 297 | WP_001275298.1 | hypothetical protein | - |
| B2H83_RS26565 () | 142649..142825 | - | 177 | WP_001054898.1 | hypothetical protein | - |
| B2H83_RS26195 () | 143217..143426 | + | 210 | WP_000062603.1 | HEAT repeat domain-containing protein | - |
| B2H83_RS26570 () | 143498..144147 | - | 650 | Protein_159 | plasmid IncI1-type surface exclusion protein ExcA | - |
| B2H83_RS26205 () | 144221..146389 | - | 2169 | WP_129542158.1 | DotA/TraY family protein | - |
| B2H83_RS26210 () | 146486..147070 | - | 585 | WP_129542159.1 | IncI1-type conjugal transfer protein TraX | - |
| B2H83_RS26215 () | 147099..148301 | - | 1203 | WP_129542160.1 | IncI1-type conjugal transfer protein TraW | - |
| B2H83_RS26220 () | 148268..148882 | - | 615 | WP_000337394.1 | IncI1-type conjugal transfer protein TraV | - |
| B2H83_RS26225 () | 148882..151926 | - | 3045 | WP_012783939.1 | IncI1-type conjugal transfer protein TraU | - |
| B2H83_RS26230 () | 152016..152816 | - | 801 | WP_064198685.1 | IncI1-type conjugal transfer protein TraT | - |
| B2H83_RS26235 () | 152800..152988 | - | 189 | WP_001277255.1 | putative conjugal transfer protein TraS | - |
| B2H83_RS26240 () | 153052..153456 | - | 405 | WP_000086955.1 | conjugal transfer protein TraR | - |
| B2H83_RS26245 () | 153506..154033 | - | 528 | WP_022630880.1 | conjugal transfer protein TraQ | - |
| B2H83_RS26250 () | 154033..154737 | - | 705 | WP_129542161.1 | IncI1-type conjugal transfer protein TraP | - |
| B2H83_RS26255 () | 154737..156026 | - | 1290 | WP_001271991.1 | conjugal transfer protein TraO | - |
| B2H83_RS26260 () | 156029..157012 | - | 984 | WP_001191880.1 | hypothetical protein | VF |
| B2H83_RS26265 () | 157055..158668 | - | 1614 | WP_000080195.1 | IS66 family transposase ISEc23 | Transposase |
| B2H83_RS26270 () | 158699..159049 | - | 351 | WP_000624722.1 | IS66 family transposase ISEc23 | Transposase |
| B2H83_RS26275 () | 159046..159471 | - | 426 | WP_000422741.1 | IS66 family transposase ISEc23 | Transposase |
| B2H83_RS26280 () | 159559..160020 | + | 462 | Protein_175 | conjugal transfer protein TraB | - |
Similar MGE(s)
Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE.
| Detail | Organism | MGE type | Related TA | Genome accession | Coordinates | Mash distance |
|---|