Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 65900..66435 Replicon plasmid unnamed1
Accession NZ_CP019954
Organism Escherichia coli M8

Toxin (Protein)


Gene name relE Uniprot ID Q60228
Locus tag B2H83_RS25720 Protein ID WP_001270417.1
Coordinates 66148..66435 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID L4J0V6
Locus tag B2H83_RS25715 Protein ID WP_001132895.1
Coordinates 65900..66151 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B2H83_RS25690 61729..63408 + 1680 WP_061892152.1 glycoside hydrolase family 43 protein -
B2H83_RS25695 63916..64098 + 183 Protein_71 IS3 family transposase -
B2H83_RS25705 64176..64448 + 273 WP_011310366.1 hypothetical protein -
B2H83_RS25710 64429..65634 + 1206 Protein_73 IS91 family transposase -
B2H83_RS25715 65900..66151 + 252 WP_001132895.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
B2H83_RS25720 66148..66435 + 288 WP_001270417.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
B2H83_RS25725 66434..66715 + 282 Protein_76 transposase -
B2H83_RS25735 67350..68327 + 978 WP_061892150.1 RepB family plasmid replication initiator protein -
B2H83_RS25740 68606..69346 - 741 WP_001066941.1 tyrosine-type recombinase/integrase -
B2H83_RS25750 70205..71047 - 843 WP_077698982.1 AraC family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..160021 160021
- inside IScluster/Tn - - 54442..66715 12273


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11214.24 Da        Isoelectric Point: 10.4703

>T73596 WP_001270417.1 NZ_CP019954:66148-66435 [Escherichia coli M8]
MRYQVKFREDALKEWQKLDKAIQQQFAKKLKKCCDNPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDEQLIIAVVAVGK
RERSDVYNLASERMR

Download         Length: 288 bp

>T73596 NZ_CP019954:66148-66435 [Escherichia coli M8]
ATGAGGTATCAGGTAAAATTCAGGGAAGATGCGCTGAAAGAGTGGCAAAAACTGGATAAGGCTATTCAGCAACAGTTTGC
GAAAAAGCTAAAAAAGTGCTGTGACAATCCGCATATTCCTTCCGCAAAACTGCGCGGGATAAAGGACTGCTACAAAATAA
AATTACGTGCGTCAGGTTTTCGCCTGGTCTATCAGGTGATTGACGAACAATTAATTATCGCTGTTGTAGCTGTGGGTAAA
CGTGAGCGAAGTGACGTTTATAATCTTGCCAGCGAAAGAATGAGATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9178.47 Da        Isoelectric Point: 4.2108

>AT73596 WP_001132895.1 NZ_CP019954:65900-66151 [Escherichia coli M8]
MPNIILSDTSASVSELKKNPMATVSAGDGFPVAILNRNQPAFYCVPAELYEKMLDALDDQELVKLVAERSNQPLHDVDLD
KYL

Download         Length: 252 bp

>AT73596 NZ_CP019954:65900-66151 [Escherichia coli M8]
ATGCCTAACATTATTCTCAGTGATACAAGCGCCAGTGTCAGCGAGCTGAAGAAAAACCCGATGGCGACAGTCAGCGCCGG
TGATGGTTTCCCGGTCGCTATCCTGAACCGTAATCAGCCTGCTTTCTACTGTGTACCCGCAGAGCTGTACGAAAAGATGC
TCGATGCCCTGGACGATCAGGAGCTGGTTAAACTGGTAGCCGAACGCAGCAACCAACCGCTGCATGATGTAGACCTGGAT
AAATACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5T8BSB4

Antitoxin

Source ID Structure
AlphaFold DB L4J0V6

References