T4SS ID	96
Strain	Escherichia coli SMS-3-5
Replicon	plasmid pSMS35_130 [Browse all T4SS(s) in this replicon]
Accession	NC_010488
Location	5088..37805
Name	Tra_F
Function	conjugation
Classification	Type IVA; Type F
This T4SS has been mentioned in a genome sequencing report.

T4SS components

Component	TraA_F	TraB_F	TraC_F	TraD_F	TraE_F	TraF_F	TraG_F	TraH_F	TraI_F
Number	1	1	1	1	1	1	1	1	1
Component	TraK_F	TraL_F	TraM_F	TraN_F	TraQ_F	TraU_F	TraV_F	TraW_F	TraX_F
Number	1	1	1	1	1	1	1	1	1
Component	TraY_F	TrbC_F	Orf169_F
Number	1	1	1

The information of T4SS components from NC_010488
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Component
1	EcSMS35_A0001	154..540 [-], 387	170650844	replication initiation protein repA4
2	EcSMS35_A0002 (repA1)	904..1773 [-], 870	170650780	replication protein
3	EcSMS35_A0003 (repA2)	2054..2314 [-], 261	170650796	replication protein
4	EcSMS35_A0005	3164..3373 [-], 210	170650792	hypothetical protein
5	EcSMS35_A0006	3419..3880 [-], 462	170650855	nuclease homolog
6	EcSMS35_A0007	4123..4335 [-], 213	170650817	hypothetical protein
7	EcSMS35_A0008 (finO)	4473..5084 [-], 612	170650904	conjugal transfer fertility inhibition protein FinO
8	EcSMS35_A0009 (traX)	5088..5834 [-], 747	170650820	conjugal transfer pilus acetylation protein TraX	TraX_F
9	EcSMS35_A0010 (traI)	5854..11124 [-], 5271	170650784	conjugal transfer nickase/helicase TraI	TraI_F
10	EcSMS35_A0011 (traD)	11124..13295 [-], 2172	170650889	conjugal transfer protein TraD	TraD_F
11	EcSMS35_A0012 (traT)	13548..14279 [-], 732	170650825	conjugal transfer surface exclusion protein TraT
12	EcSMS35_A0013	14311..14808 [-], 498	170650886	putative type IV secretion-like conjugative transfer system protein TraS
13	EcSMS35_A0014 (traG)	14829..17651 [-], 2823	170650902	conjugal transfer mating pair stabilization protein TraG	TraG_F
14	EcSMS35_A0015 (traH)	17648..19024 [-], 1377	170650800	conjugal transfer pilus assembly protein TraH	TraH_F
15	EcSMS35_A0016 (trbJ)	19021..19383 [-], 363	170650880	conjugal transfer protein TrbJ
16	EcSMS35_A0017 (trbB)	19313..19858 [-], 546	170650781	conjugal transfer protein TrbB
17	EcSMS35_A0018 (traQ)	19845..20129 [-], 285	170650897	conjugal transfer pilin chaperone TraQ	TraQ_F
18	EcSMS35_A0019 (trbA)	20248..20595 [-], 348	170650822	conjugal transfer protein TrbA
19	EcSMS35_A0020 (traF)	20611..21354 [-], 744	170650828	conjugal pilus assembly protein TraF	TraF_F
20	EcSMS35_A0021 (trbE)	21347..21604 [-], 258	170650840	conjugal transfer protein TrbE
21	EcSMS35_A0022 (traN)	21631..23439 [-], 1809	170650771	conjugal transfer mating pair stabilization protein TraN	TraN_F
22	EcSMS35_A0023 (trbC)	23436..24074 [-], 639	170650809	conjugal transfer pilus assembly protein TrbC	TrbC_F
23	EcSMS35_A0024	24102..24554 [-], 453	170650862	hypothetical protein
24	EcSMS35_A0025	24584..25045 [-], 462	170650772	hypothetical protein
25	EcSMS35_A0026 (traU)	25071..26063 [-], 993	170650911	conjugal transfer pilus assembly protein TraU	TraU_F
26	EcSMS35_A0027 (traW)	26060..26692 [-], 633	170650832	conjugal transfer pilus assembly protein TraW	TraW_F
27	EcSMS35_A0028 (trbI)	26689..27075 [-], 387	170650810	conjugal transfer protein TrbI
28	EcSMS35_A0029 (traC)	27072..29699 [-], 2628	170650779	conjugal transfer ATP-binding protein TraC	TraC_F
29	EcSMS35_A0030 (traR)	29859..30080 [-], 222	170650873	conjugal transfer protein TraR
30	EcSMS35_A0031 (traV)	30215..30730 [-], 516	170650794	conjugal transfer protein TraV	TraV_F
31	EcSMS35_A0032	30727..30978 [-], 252	170650806	conjugal transfer protein TrbG
32	EcSMS35_A0033	30975..31292 [-], 318	170650884	conjugal transfer protein TrbD
33	EcSMS35_A0034 (traP)	31289..31861 [-], 573	170650891	conjugal transfer protein TraP
34	EcSMS35_A0035 (traB)	31851..33278 [-], 1428	170650812	conjugal transfer pilus assembly protein TraB	TraB_F
35	EcSMS35_A0036 (traK)	33278..34006 [-], 729	170650906	conjugal transfer protein TraK	TraK_F
36	EcSMS35_A0037 (traE)	33993..34559 [-], 567	170650797	conjugal transfer pilus assembly protein TraE	TraE_F
37	EcSMS35_A0038 (traL)	34581..34892 [-], 312	170650856	conjugal transfer pilus assembly protein TraL	TraL_F
38	EcSMS35_A0039 (traA)	34907..35266 [-], 360	170650824	conjugal transfer pilin subunit TraA	TraA_F
39	EcSMS35_A0040 (traY)	35300..35527 [-], 228	170650869	conjugal transfer protein TraY	TraY_F
40	EcSMS35_A0041 (traJ)	35621..36307 [-], 687	170650783	type IV secretion-like conjugative transfer system regulator TraJ
41	EcSMS35_A0042 (traM)	36498..36881 [-], 384	170650851	conjugal transfer protein TraM	TraM_F
42	EcSMS35_A0043	37158..37805 [+], 648	170650864	lytic transglycosylase	Orf169_F
43	EcSMS35_A0044	37831..37965 [-], 135	170650793	hypothetical protein
44	EcSMS35_A0045	38102..38923 [-], 822	170650799	hypothetical protein
45	EcSMS35_A0046	39046..39333 [-], 288	170650769	hypothetical protein
46	EcSMS35_A0047	39358..39519 [-], 162	170650803	hypothetical protein
47	EcSMS35_A0048	39600..39812 [-], 213	170650770	hypothetical protein
48	EcSMS35_A0049	39805..39957 [-], 153	170650839	hypothetical protein
49	EcSMS35_A0050 (hok)	40255..40413 [-], 159	170650845	small toxic polypeptide
50	EcSMS35_A0051	40556..40693 [-], 138	170650801	hypothetical protein
51	EcSMS35_A0052 (psiA)	40693..41412 [-], 720	170650870	plasmid SOS inhibition protein A
52	EcSMS35_A0053 (psiB)	41409..41843 [-], 435	170650814	plasmid SOS inhibition protein B

 This T4SS contains information of accessory protein.

Genes in the 5-Kb flanking regions if available, or non-essential genes in the T4SS gene cluster if any.

Download FASTA format files

Proteins        Genes

The information on requirements for T4SS substrate-channel docking

Certain T4SS substrates require secretion chaperones for translocation. These chaperones often possess physical properties (small size of 15 kDa, acidic pI, and amphipathic helices) resembling those of chaperones associated with the type III secretion systems, a family of macromolecular translocation systems ancestrally related to bacterial flagella.

#	Accessory protein(GI)	motif(s)	Substrate(s)	Function	Reference
1	TraM (170650851)	ND	TraI	For transfer initiation, traM mediates signalling between the relaxosome and transferosome.	(1) PubMed: 22788760

Tips:
1.Substrate(s): For the conjugation systems, the listed proteins are relaxases that bind a cognate T4CP and are delivered to recipient cells. For the effector translocator systems, the listed proteins are effectors that play a role in the infection processes of the bacterial pathogen.
2.motif(s):The motifs listed are required for substrate translocation. In some cases, the protein or its C-terminal fragment (CT) is sufficient to mediate translocation to target cells, as shown by fusion to a reporter protein such as Cre recombinase or adenylate cyclase. Amino acids (aa) at positions listed relative to the C-terminal fragment (subscript) are required for translocation, as shown by mutational analysis. ND, not determined. Parentheses indicate that the interaction between a protein substrate and a cognate T4CP has been experimentally shown.
3.Accessory protein: Accessory factors required for T4SS channel docking or translocation. The proposed function in mediating substrate-T4SS channel docking is shown in parentheses.PubMed:19946141

(1) Wong JJ; Lu J; Glover JN (2012). Relaxosome function and conjugation regulation in F-like plasmids - a structural biology perspective. Mol Microbiol. 85(4):602-17. [PudMed:22788760]

(1) Fricke WF; Wright MS; Lindell AH; Harkins DM; Baker-Austin C; Ravel J; Stepanauskas R (2008). Insights into the environmental resistance gene pool from the genome sequence of the multidrug-resistant environmental isolate Escherichia coli SMS-3-5. J Bacteriol. 190(20):6779-94. [PudMed:18708504]