StrainEscherichia coli SMS-3-5
Repliconplasmid pSMS35_130 [Browse all T4SS(s) in this replicon]
ClassificationType IVA; Type F
sequence This T4SS has been mentioned in a genome sequencing report.

T4SS components

The information of T4SS components from NC_010488
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductComponent
1EcSMS35_A0001154..540 [-], 387170650844replication initiation protein repA4 
2EcSMS35_A0002 (repA1)904..1773 [-], 870170650780replication protein 
3EcSMS35_A0003 (repA2)2054..2314 [-], 261170650796replication protein 
4EcSMS35_A00053164..3373 [-], 210170650792hypothetical protein 
5EcSMS35_A00063419..3880 [-], 462170650855nuclease homolog 
6EcSMS35_A00074123..4335 [-], 213170650817hypothetical protein 
7EcSMS35_A0008 (finO)4473..5084 [-], 612170650904conjugal transfer fertility inhibition protein FinO 
8EcSMS35_A0009 (traX)5088..5834 [-], 747170650820conjugal transfer pilus acetylation protein TraX  TraX_F
9EcSMS35_A0010 (traI)5854..11124 [-], 5271170650784conjugal transfer nickase/helicase TraI  TraI_F
10EcSMS35_A0011 (traD)11124..13295 [-], 2172170650889conjugal transfer protein TraD  TraD_F
11EcSMS35_A0012 (traT)13548..14279 [-], 732170650825conjugal transfer surface exclusion protein TraT 
12EcSMS35_A001314311..14808 [-], 498170650886putative type IV secretion-like conjugative transfer system protein TraS 
13EcSMS35_A0014 (traG)14829..17651 [-], 2823170650902conjugal transfer mating pair stabilization protein TraG  TraG_F
14EcSMS35_A0015 (traH)17648..19024 [-], 1377170650800conjugal transfer pilus assembly protein TraH  TraH_F
15EcSMS35_A0016 (trbJ)19021..19383 [-], 363170650880conjugal transfer protein TrbJ 
16EcSMS35_A0017 (trbB)19313..19858 [-], 546170650781conjugal transfer protein TrbB 
17EcSMS35_A0018 (traQ)19845..20129 [-], 285170650897conjugal transfer pilin chaperone TraQ  TraQ_F
18EcSMS35_A0019 (trbA)20248..20595 [-], 348170650822conjugal transfer protein TrbA 
19EcSMS35_A0020 (traF)20611..21354 [-], 744170650828conjugal pilus assembly protein TraF  TraF_F
20EcSMS35_A0021 (trbE)21347..21604 [-], 258170650840conjugal transfer protein TrbE 
21EcSMS35_A0022 (traN)21631..23439 [-], 1809170650771conjugal transfer mating pair stabilization protein TraN  TraN_F
22EcSMS35_A0023 (trbC)23436..24074 [-], 639170650809conjugal transfer pilus assembly protein TrbC  TrbC_F
23EcSMS35_A002424102..24554 [-], 453170650862hypothetical protein 
24EcSMS35_A002524584..25045 [-], 462170650772hypothetical protein 
25EcSMS35_A0026 (traU)25071..26063 [-], 993170650911conjugal transfer pilus assembly protein TraU  TraU_F
26EcSMS35_A0027 (traW)26060..26692 [-], 633170650832conjugal transfer pilus assembly protein TraW  TraW_F
27EcSMS35_A0028 (trbI)26689..27075 [-], 387170650810conjugal transfer protein TrbI 
28EcSMS35_A0029 (traC)27072..29699 [-], 2628170650779conjugal transfer ATP-binding protein TraC  TraC_F
29EcSMS35_A0030 (traR)29859..30080 [-], 222170650873conjugal transfer protein TraR 
30EcSMS35_A0031 (traV)30215..30730 [-], 516170650794conjugal transfer protein TraV  TraV_F
31EcSMS35_A003230727..30978 [-], 252170650806conjugal transfer protein TrbG 
32EcSMS35_A003330975..31292 [-], 318170650884conjugal transfer protein TrbD 
33EcSMS35_A0034 (traP)31289..31861 [-], 573170650891conjugal transfer protein TraP 
34EcSMS35_A0035 (traB)31851..33278 [-], 1428170650812conjugal transfer pilus assembly protein TraB  TraB_F
35EcSMS35_A0036 (traK)33278..34006 [-], 729170650906conjugal transfer protein TraK  TraK_F
36EcSMS35_A0037 (traE)33993..34559 [-], 567170650797conjugal transfer pilus assembly protein TraE  TraE_F
37EcSMS35_A0038 (traL)34581..34892 [-], 312170650856conjugal transfer pilus assembly protein TraL  TraL_F
38EcSMS35_A0039 (traA)34907..35266 [-], 360170650824conjugal transfer pilin subunit TraA  TraA_F
39EcSMS35_A0040 (traY)35300..35527 [-], 228170650869conjugal transfer protein TraY  TraY_F
40EcSMS35_A0041 (traJ)35621..36307 [-], 687170650783type IV secretion-like conjugative transfer system regulator TraJ 
41EcSMS35_A0042 (traM)36498..36881 [-], 384170650851conjugal transfer protein TraM  TraM_Faccesspry protein
42EcSMS35_A004337158..37805 [+], 648170650864lytic transglycosylase  Orf169_F
43EcSMS35_A004437831..37965 [-], 135170650793hypothetical protein 
44EcSMS35_A004538102..38923 [-], 822170650799hypothetical protein 
45EcSMS35_A004639046..39333 [-], 288170650769hypothetical protein 
46EcSMS35_A004739358..39519 [-], 162170650803hypothetical protein 
47EcSMS35_A004839600..39812 [-], 213170650770hypothetical protein 
48EcSMS35_A004939805..39957 [-], 153170650839hypothetical protein 
49EcSMS35_A0050 (hok)40255..40413 [-], 159170650845small toxic polypeptide 
50EcSMS35_A005140556..40693 [-], 138170650801hypothetical protein 
51EcSMS35_A0052 (psiA)40693..41412 [-], 720170650870plasmid SOS inhibition protein A 
52EcSMS35_A0053 (psiB)41409..41843 [-], 435170650814plasmid SOS inhibition protein B 
accesspry protein This T4SS contains information of accessory protein.
flank Genes in the 5-Kb flanking regions if available, or non-essential genes in the T4SS gene cluster if any.

Download FASTA format files
Proteins        Genes
The information on requirements for T4SS substrate-channel docking

Certain T4SS substrates require secretion chaperones for translocation. These chaperones often possess physical properties (small size of 15 kDa, acidic pI, and amphipathic helices) resembling those of chaperones associated with the type III secretion systems, a family of macromolecular translocation systems ancestrally related to bacterial flagella.

#Accessory protein(GI)motif(s)Substrate(s)FunctionReference
1TraM (170650851)NDTraIFor transfer initiation, traM mediates signalling between the relaxosome and transferosome.(1) PubMed: 22788760
1.Substrate(s): For the conjugation systems, the listed proteins are relaxases that bind a cognate T4CP and are delivered to recipient cells. For the effector translocator systems, the listed proteins are effectors that play a role in the infection processes of the bacterial pathogen.
2.motif(s):The motifs listed are required for substrate translocation. In some cases, the protein or its C-terminal fragment (CT) is sufficient to mediate translocation to target cells, as shown by fusion to a reporter protein such as Cre recombinase or adenylate cyclase. Amino acids (aa) at positions listed relative to the C-terminal fragment (subscript) are required for translocation, as shown by mutational analysis. ND, not determined. Parentheses indicate that the interaction between a protein substrate and a cognate T4CP has been experimentally shown.
3.Accessory protein: Accessory factors required for T4SS channel docking or translocation. The proposed function in mediating substrate-T4SS channel docking is shown in parentheses.PubMed:19946141

(1) Wong JJ; Lu J; Glover JN (2012). Relaxosome function and conjugation regulation in F-like plasmids - a structural biology perspective. Mol Microbiol. 85(4):602-17. [PudMed:22788760]
(1) Fricke WF; Wright MS; Lindell AH; Harkins DM; Baker-Austin C; Ravel J; Stepanauskas R (2008). Insights into the environmental resistance gene pool from the genome sequence of the multidrug-resistant environmental isolate Escherichia coli SMS-3-5. J Bacteriol. 190(20):6779-94. [PudMed:18708504]