SecReT4
T4SS ID95
StrainEscherichia coli 1520 plasmid pIP1206 (NC_010558)
Repliconplasmid pIP1206 [Browse all T4SS(s) in this replicon]
AccessionNC_010558
Location37256..69612
NameTra_F
Functionconjugation
ClassificationType IVA; Type F
sequence This T4SS has been mentioned in a genome sequencing report.

T4SS components
ComponentTraA_FTraB_FTraC_FTraD_FTraE_FTraF_FTraG_FTraH_FTraI_F
Number111111111
ComponentTraK_FTraL_FTraM_FTraN_FTraQ_FTraU_FTraV_FTraW_FTraX_F
Number111111111
ComponentTraY_FTrbC_FOrf169_F
Number111

The information of T4SS components from NC_010558
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductComponent
1IPF_33634676..34834 [+], 159187736776small toxic polypeptide 
2IPF_334 (YubN)35072..35389 [-], 318187736777YubN 
3IPF_331 (YubO)35733..36020 [+], 288187736778YubO 
4IPF_329 (YubP)36139..36960 [+], 822187736779YubP 
5IPF_32837256..37846 [-], 591187736780lytic transglycosylase  Orf169_F
6IPF_327 (TraM)38198..38581 [+], 384187736781conjugal transfer protein TraM  TraM_Faccesspry protein
7IPF_326 (TraJ)38772..39419 [+], 648187736782conjugal transfer transcriptional regulator TraJ 
8IPF_396 (TraY)39555..39770 [+], 216187736783conjugal transfer protein TraY  TraY_F
9IPF_325 (TraA)39813..40178 [+], 366187736784conjugal transfer pilin subunit TraA  TraA_F
10IPF_323 (TraL)40193..40504 [+], 312187736785conjugal transfer pilus assembly protein TraL  TraL_F
11IPF_322 (TraE)40526..41092 [+], 567187736786conjugal transfer pilus assembly protein TraE  TraE_F
12IPF_321 (TraK)41079..41807 [+], 729187736787conjugal transfer protein TraK  TraK_F
13IPF_318 (TraB)41807..43234 [+], 1428187736788conjugal transfer pilus assembly protein TraB  TraB_F
14IPF_315 (TraP)43224..43814 [+], 591187736789conjugal transfer protein TraP 
15IPF_314 (TrbG)44114..44365 [+], 252187736790conjugal transfer protein TrbG 
16IPF_312 (TraV)44362..44877 [+], 516187736791conjugal transfer protein TraV  TraV_F
17IPF_309 (TraC)45393..48020 [+], 2628187736792conjugal transfer ATP-binding protein TraC  TraC_F
18IPF_306a (TrbI)48017..48403 [+], 387187736793conjugal transfer protein TrbI 
19IPF_304 (TraW)48286..49032 [+], 747187736794conjugal transfer pilus assembly protein TraW  TraW_F
20IPF_303 (TraU)49029..50021 [+], 993187736795conjugal transfer pilus assembly protein TraU  TraU_F
21IPF_301 (TrbC)50027..50668 [+], 642187736796conjugal transfer pilus assembly protein TrbC  TrbC_F
22IPF_299 (TraN)50665..52473 [+], 1809187736797conjugal transfer mating pair stabilization protein TraN  TraN_F
23IPF_397 (TrbE)52497..52757 [+], 261187736798conjugal transfer protein TrbE 
24IPF_297 (TraF)52720..53493 [+], 774187736799conjugal pilus assembly protein TraF  TraF_F
25IPF_295 (TrbA)53507..53848 [+], 342187736800conjugal transfer protein TrbA 
26IPF_294 (ArtA)53829..54164 [-], 336187736801ArtA 
27IPF_293 (TraQ)54245..54529 [+], 285187736802conjugal transfer pilin chaperone TraQ  TraQ_F
28IPF_291 (TrbB)54516..55070 [+], 555187736803conjugal transfer protein TrbB 
29IPF_405 (TrbJ)55000..55284 [+], 285187736804conjugal transfer protein TrbJ 
30IPF_290 (TrbF)55327..55719 [+], 393187736805conjugal transfer protein TrbF 
31IPF_289 (TraH)55703..57079 [+], 1377187736806conjugal transfer pilus assembly protein TraH  TraH_F
32IPF_286 (TraG)57076..59901 [+], 2826187736807conjugal transfer mating pair stabilization protein TraG  TraG_F
33IPF_284 (TraS)59898..60353 [+], 456187736808conjugal transfer entry exclusion protein TraS 
34IPF_283 (TraT)60422..61153 [+], 732187736809conjugal transfer surface exclusion protein TraT 
35IPF_282 (TraD)61405..63576 [+], 2172187736810conjugal transfer protein TraD  TraD_F
36IPF_279a (TraI)63576..68846 [+], 5271187736811conjugal transfer nickase/helicase TraI  TraI_F
37IPF_270a (TraX)68809..69612 [+], 804187736812conjugal transfer pilus acetylation protein TraX  TraX_F
38IPF_268 (FinO)69667..70227 [+], 561187736813conjugal transfer fertility inhibition protein FinO 
39IPF_267 (YigA)70360..70572 [+], 213187736814YigA 
40IPF_265 (IS1-insB)70873..71259 [-], 387187736815IS1-insB 
41IPF_264 (IS1-insA)71300..71575 [-], 276187736816IS1-insA 
42IPF_386 (SrnB)71940..72251 [+], 312187736817SrnB 
43IPF_384 (RepA2)72535..72792 [+], 258187736818replication protein 
44IPF_387 (RepA6)73028..73102 [+], 75187736819leader peptide RepL 
45IPF_260a (RepA1)73095..73547 [+], 453187736820RepA1 
46IPF_25873596..74051 [+], 456187736821hypothetical protein 
 
accesspry protein This T4SS contains information of accessory protein.
flank Genes in the 5-Kb flanking regions if available, or non-essential genes in the T4SS gene cluster if any.

Download FASTA format files
Proteins        Genes
The information on requirements for T4SS substrate-channel docking

Certain T4SS substrates require secretion chaperones for translocation. These chaperones often possess physical properties (small size of 15 kDa, acidic pI, and amphipathic helices) resembling those of chaperones associated with the type III secretion systems, a family of macromolecular translocation systems ancestrally related to bacterial flagella.

#Accessory protein(GI)motif(s)Substrate(s)FunctionReference
1TraM (187736781)NDTraIFor transfer initiation, traM mediates signalling between the relaxosome and transferosome.(1) PubMed: 22788760
Tips:
1.Substrate(s): For the conjugation systems, the listed proteins are relaxases that bind a cognate T4CP and are delivered to recipient cells. For the effector translocator systems, the listed proteins are effectors that play a role in the infection processes of the bacterial pathogen.
2.motif(s):The motifs listed are required for substrate translocation. In some cases, the protein or its C-terminal fragment (CT) is sufficient to mediate translocation to target cells, as shown by fusion to a reporter protein such as Cre recombinase or adenylate cyclase. Amino acids (aa) at positions listed relative to the C-terminal fragment (subscript) are required for translocation, as shown by mutational analysis. ND, not determined. Parentheses indicate that the interaction between a protein substrate and a cognate T4CP has been experimentally shown.
3.Accessory protein: Accessory factors required for T4SS channel docking or translocation. The proposed function in mediating substrate-T4SS channel docking is shown in parentheses.PubMed:19946141

(1) Wong JJ; Lu J; Glover JN (2012). Relaxosome function and conjugation regulation in F-like plasmids - a structural biology perspective. Mol Microbiol. 85(4):602-17. [PudMed:22788760]
(1) Perichon B; Bogaerts P; Lambert T; Frangeul L; Courvalin P; Galimand M (2008). Sequence of conjugative plasmid pIP1206 mediating resistance to aminoglycosides by 16S rRNA methylation and to hydrophilic fluoroquinolones by efflux. Antimicrob Agents Chemother. 52(7):2581-92. [PudMed:18458128]
 

Genomics & Bioinformatics Group in MML, SJTU