T4SS ID569
StrainSalmonella enterica subsp. enterica serovar Typhi plasmid R27 (NC_002305)
Repliconplasmid R27 [Browse all T4SS(s) in this replicon]
Location2116..10886; 28465..38038; 101815..118708
ClassificationType IVA; Type F
experimental Experimental investigation has been performed on this T4SS.

T4SS components

The information of T4SS components from NC_002305
Region 1: 2116..10886
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductComponent
1R27_p002 (R0001)407..784 [-], 37810957191hypothetical protein 
2R27_p003 (R0002)843..1718 [-], 87610957192hypothetical protein 
3R27_p004 (trhI)2116..3900 [-], 178510957193putative DNA helicase  TraI_F
4R27_p005 (R0004)4082..4954 [+], 87310957194hypothetical protein 
5R27_p006 (trhN)4982..8158 [-], 317710957195conjugal transfer mating pair stabilization protein TraN  TraN_F
6R27_p007 (trhU)8181..9128 [-], 94810957196putative sex pilus assembly and synthesis  TraU_F
7R27_p008 (trhW)9378..10886 [-], 150910957197putative transfer protein, pilus formation  TraW_F
8R27_p009 (trhF)10873..11385 [-], 51310957198putative signal peptidase I 
9R27_p010 (R0009)11659..15879 [-], 422110957199hypothetical protein 
Region 2: 28465..38038
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductComponent
1R27_p021 (R0020)24085..25338 [-], 125410957210putative partition protein 
2R27_p022 (R0021)26188..26595 [-], 40810957211hypothetical protein 
3R27_p023 (R0022)27134..27238 [-], 10510957212hypothetical protein 
4R27_p024 (R0023)27477..27797 [-], 32110957213hypothetical protein 
5R27_p025 (trhC)28465..31146 [-], 268210957214putative transfer protein  TraC_F
6R27_p026 (trhV)31155..32105 [-], 95110957215putative transfer protein  TraV_F
7R27_p027 (htdT)32115..32867 [-], 75310957216putative transfer protein 
8R27_p028 (R0028)32973..33485 [-], 51310957217hypothetical protein 
9R27_p029 (trhB)33494..34852 [-], 135910957218putative transfer protein  TraB_F
10R27_p030 (R0030)34842..35282 [-], 44110957219hypothetical protein 
11R27_p031 (R0031)35284..36516 [-], 123310957220hypothetical protein  TraK_F
12R27_p032 (trhE)36519..37304 [-], 78610957221hypothetical protein  TraE_F
13R27_p033 (trhL)37316..37633 [-], 31810957222hypothetical protein  TraL_F
14R27_p034 (trhA)37685..38038 [-], 35410957223hypothetical protein  TraA_F
15R27_p035 (R0035)38633..39226 [+], 59410957224hypothetical protein 
16R27_p036 (repHIA)39480..40355 [-], 87610957225replication initiation protein 
17R27_p037 (R0037)40890..41966 [+], 107710957226hypothetical protein 
18R27_p038 (R0038)41984..42184 [+], 20110957227hypothetical protein 
19R27_p039 (R0039)42181..42888 [+], 70810957228hypothetical protein 
Region 3: 101815..118708
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductComponent
1R27_p113 (R0113)97355..97669 [+], 31510957302hypothetical protein 
2R27_p114 (R0114)97756..98112 [+], 35710957303hypothetical protein 
3R27_p115 (R0115)98139..98867 [-], 72910957304hypothetical protein 
4R27_p116 (R0116)98869..100050 [-], 118210957305hypothetical protein 
5R27_p117 (R0117)100063..100725 [-], 66310957306hypothetical protein 
6R27_p118 (R0118)100712..101815 [-], 110410957307hypothetical protein 
7R27_p119 (traG)101815..103899 [-], 208510957308hypothetical protein  TraD_F
8R27_p120 (traI)103899..106934 [-], 303610957309hypothetical protein 
9R27_p121 (R0121)106945..107667 [-], 72310957310hypothetical protein 
10R27_p122 (R0122)107664..108149 [-], 48610957311hypothetical protein 
11R27_p123 (R0123)108907..109707 [+], 80110957312hypothetical protein 
12R27_p124 (R0124)109710..110222 [+], 51310957313hypothetical protein 
13R27_p125 (R0125)110782..110910 [+], 12910957314hypothetical protein 
14R27_p126 (R0126)110834..111880 [+], 104710957315putative pilus assembly and synthesis protein  TraF_F
15R27_p127 (trhH)111870..113285 [+], 141610957316putative F pilus assembly protein  TraH_F
16R27_p128 (R0128)113294..117283 [+], 399010957317hypothetical protein  TraG_F
17R27_p129 (R0129)117530..118141 [-], 61210957318hypothetical protein 
18R27_p130 (bfpH)118193..118708 [-], 51610957319putative BfpH/trbN- like protein  Orf169_F
19R27_p131 (dsbC)118856..119605 [+], 75010957320thiol:disulphide interchange protein 
20R27_p132 (R0132)119631..120023 [+], 39310957321hypothetical protein 
21R27_p133 (R0133)120046..120468 [+], 42310957322hypothetical protein 
22R27_p134 (R0134)120527..121138 [-], 61210957323hypothetical protein 
23R27_p135 (R0135)121245..122054 [+], 81010957324putative outer membrane protein 
24R27_p136 (mucB)122106..123314 [-], 120910957325putative UV protection protein 
flank Genes in the 5-Kb flanking regions if available, or non-essential genes in the T4SS gene cluster if any.

Download FASTA format files
Proteins        Genes
(1) Gunton JE; Gilmour MW; Alonso G; Taylor DE (2005). Subcellular localization and functional domains of the coupling protein, TraG, from IncHI1 plasmid R27. Microbiology. 151(Pt 11):3549-61. [PudMed:16272378] experimental
(2) Gilmour MW; Gunton JE; Lawley TD; Taylor DE (2003). Interaction between the IncHI1 plasmid R27 coupling protein and type IV secretion system: TraG associates with the coiled-coil mating pair formation protein TrhB. Mol Microbiol. 49(1):105-16. [PudMed:12823814] experimental
(3) Lawley TD; Gilmour MW; Gunton JE; Tracz DM; Taylor DE (2003). Functional and mutational analysis of conjugative transfer region 2 (Tra2) from the IncHI1 plasmid R27. J Bacteriol. 185(2):581-91. [PudMed:12511505] experimental
(4) Sherburne CK; Lawley TD; Gilmour MW; Blattner FR; Burland V; Grotbeck E; Rose DJ; Taylor DE (2000). The complete DNA sequence and analysis of R27, a large IncHI plasmid from Salmonella typhi that is temperature sensitive for transfer. Nucleic Acids Res. 28(10):2177-86. [PudMed:10773089]
experimental This literature contains experimental investigation