T4SS ID532
StrainEscherichia coli ETEC H10407 plasmid pEntH10407 (NC_013507)
Repliconplasmid pEntH10407 [Browse all T4SS(s) in this replicon]
ClassificationType IVA; Type F
experimental Experimental investigation has been performed on this T4SS.

T4SS components

The information of T4SS components from NC_013507
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductComponent
1pEntH10407_p65 (repA4)40185..40370 [-], 186268507224replication protein RepA4 
2pEntH10407_p6640703..40873 [-], 171268507225hypothetical protein 
3pEntH10407_p67 (repA1)40936..41793 [-], 858268507226replication initiation protein RepA1 
4pEntH10407_p68 (tapA)41786..41860 [-], 75268507227leader peptide TapA 
5pEntH10407_p69 (copA)41806..41937 [-], 132268507228protein copA/incA 
6pEntH10407_p70 (repA2)42094..42354 [-], 261268507229replication protein RepA2 
7pEntH10407_p7142594..43184 [-], 591268507230hypothetical protein 
8pEntH10407_p72 (rmoA)43227..43430 [-], 204268507231putative regulator 
9pEntH10407_p73 (yigB)43476..43937 [-], 462268507232micrococcal nuclease homolog 
10pEntH10407_p74 (yigA)44182..44394 [-], 213268507233hypothetical protein 
11pEntH10407_p75 (finO)44526..45086 [-], 561268507234fertility inhibition protein 
12pEntH10407_p76 (traX)45141..45944 [-], 804268507235F-pilin acetylation protein  TraX_F
13pEntH10407_p77 (traI)45907..51177 [-], 5271268507236conjugal transfer nickase/helicase  TraI_F
14pEntH10407_p78 (traD)51177..53465 [-], 2289268507237coupling protein TraD  TraD_F
15pEntH10407_p7953516..53944 [-], 429268507238hypothetical protein 
16pEntH10407_p8054075..55646 [-], 1572268507239hypothetical protein 
17pEntH10407_p8155666..56013 [-], 348268507240hypothetical protein 
18pEntH10407_p8256013..56690 [-], 678268507241hypothetical protein 
19pEntH10407_p8356910..57311 [+], 402268507242hypothetical protein 
20pEntH10407_p8457308..57655 [+], 348268507243hypothetical protein 
21pEntH10407_p8557705..59243 [+], 1539268507244hypothetical protein 
22pEntH10407_p8659377..59613 [-], 237268507245hypothetical protein 
23pEntH10407_p87 (traR)59606..59827 [-], 222268507246conjugal transfer protein TraR 
24pEntH10407_p88 (traV)59962..60477 [-], 516268507247conjugal transfer protein TraV  TraV_F
25pEntH10407_p89 (trbG)60474..60725 [-], 252268507248conjugal transfer protein TrbG 
26pEntH10407_p90 (trbD)60718..61038 [-], 321268507249conjugal transfer protein TrbD 
27pEntH10407_p91 (traP)61025..61612 [-], 588268507250conjugal transfer protein TraP 
28pEntH10407_p92 (traB)61581..63032 [-], 1452268507251conjugal transfer protein TraB  TraB_F
29pEntH10407_p93 (traK)63032..63760 [-], 729268507252conjugal transfer protein TraK  TraK_F
30pEntH10407_p94 (traE)63747..64313 [-], 567268507253conjugal transfer protein TraE  TraE_F
31pEntH10407_p95 (traL)64335..64646 [-], 312268507254conjugal transfer protein TraL  TraL_F
32pEntH10407_p96 (traA)64661..65026 [-], 366268507255prepropilin  TraA_F
33pEntH10407_p97 (traY)65058..65453 [-], 396268507256conjugal transfer protein Y  TraY_F
34pEntH10407_p98 (traJ)65552..66241 [-], 690268507257positive regulator of conjugal transfer operon 
35pEntH10407_p99 (traM)66428..66811 [-], 384268507258conjugal transfer protein TraM  TraM_Faccesspry protein
accesspry protein This T4SS contains information of accessory protein.
flank Genes in the 5-Kb flanking regions if available, or non-essential genes in the T4SS gene cluster if any.

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Proteins        Genes
The information on requirements for T4SS substrate-channel docking

Certain T4SS substrates require secretion chaperones for translocation. These chaperones often possess physical properties (small size of 15 kDa, acidic pI, and amphipathic helices) resembling those of chaperones associated with the type III secretion systems, a family of macromolecular translocation systems ancestrally related to bacterial flagella.

#Accessory protein(GI)motif(s)Substrate(s)FunctionReference
1TraM (268507258)NDTraIFor transfer initiation, traM mediates signalling between the relaxosome and transferosome.(1) PubMed: 22788760
1.Substrate(s): For the conjugation systems, the listed proteins are relaxases that bind a cognate T4CP and are delivered to recipient cells. For the effector translocator systems, the listed proteins are effectors that play a role in the infection processes of the bacterial pathogen.
2.motif(s):The motifs listed are required for substrate translocation. In some cases, the protein or its C-terminal fragment (CT) is sufficient to mediate translocation to target cells, as shown by fusion to a reporter protein such as Cre recombinase or adenylate cyclase. Amino acids (aa) at positions listed relative to the C-terminal fragment (subscript) are required for translocation, as shown by mutational analysis. ND, not determined. Parentheses indicate that the interaction between a protein substrate and a cognate T4CP has been experimentally shown.
3.Accessory protein: Accessory factors required for T4SS channel docking or translocation. The proposed function in mediating substrate-T4SS channel docking is shown in parentheses.PubMed:19946141

(1) Wong JJ; Lu J; Glover JN (2012). Relaxosome function and conjugation regulation in F-like plasmids - a structural biology perspective. Mol Microbiol. 85(4):602-17. [PudMed:22788760]
(1) Ochi S; Shimizu T; Ohtani K; Ichinose Y; Arimitsu H; Tsukamoto K; Kato M; Tsuji T (2009). Nucleotide sequence analysis of the enterotoxigenic Escherichia coli Ent plasmid. DNA Res. 16(5):299-309. [PudMed:19767599] experimental
experimental This literature contains experimental investigation