SecReT4
T4SS ID128
StrainEscherichia coli plasmid pEC_L46 (NC_014385)
Repliconplasmid pEC_L46 [Browse all T4SS(s) in this replicon]
AccessionNC_014385
Location54279..71591
NameTra_F
Functionconjugation
ClassificationType IVA; Type F
sequence This T4SS has been mentioned in a genome sequencing report.
structure Information of structure: structureTraM

T4SS components
ComponentTraA_FTraB_FTraC_FTraD_FTraE_FTraF_FTraG_FTraH_FTraI_F
Number111010000
ComponentTraK_FTraL_FTraM_FTraN_FTraQ_FTraU_FTraV_FTraW_FTraX_F
Number111111110
ComponentTraY_FTrbC_FOrf169_F
Number111

The information of T4SS components from NC_014385
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductComponent
1pECL46p055 (psiB)50243..50680 [+], 438302595387plasmid SOS inhibition protein B 
2pECL46p056 (psiA)50677..51396 [+], 720302595388plasmid SOS inhibition protein A 
3pECL46p057 (mok)51408..51638 [-], 231302595389modulator of Hok protein 
4pECL46p05952188..52412 [-], 225302595390hypothetical protein 
5pECL46p06052456..52689 [-], 234302595391hypothetical protein 
6pECL46p061 (yubP)54101..54253 [+], 153302595392hypothetical protein 
7pECL46p062 (X)54279..54956 [-], 678302595393X-polypeptide  Orf169_F
8pECL46p063 (traM)55212..55595 [+], 384302595394mating signal  TraM_F
9pECL46p064 (traJ)55729..56475 [+], 747302595395regulation 
10pECL46p065 (traY)56569..56796 [+], 228302595396OriT nicking  TraY_F
11pECL46p066 (traA)56806..57192 [+], 387302595397F pilin subunit  TraA_F
12pECL46p067 (traL)57194..57508 [+], 315302595398F pilin assembly  TraL_F
13pECL46p068 (traE)57530..58096 [+], 567302595399F pilin assembly  TraE_F
14pECL46p069 (traK)58062..58811 [+], 750302595400F pilin assembly  TraK_F
15pECL46p070 (traB)58808..60238 [+], 1431302595401F pilin assembly  TraB_F
16pECL46p073 (trbG)61097..61363 [+], 267302595402conjugal transfer protein 
17pECL46p074 (traV)61360..61875 [+], 516302595403F pilin assembly  TraV_F
18pECL46p075 (traR)62010..62231 [+], 222302595404conjugal transfer protein 
19pECL46p076 (traC)62385..65018 [+], 2634302595405F pilin assembly  TraC_F
20pECL46p077 (trbI)65000..65401 [+], 402302595406conjugal transfer protein 
21pECL46p078 (traW)65284..66030 [+], 747302595407F pilin assembly  TraW_F
22pECL46p079 (traU)66000..67019 [+], 1020302595408F pilin assembly  TraU_F
23pECL46p080 (yfdA)67043..67354 [+], 312302595409hypothetical protein 
24pECL46p081 (trbC)67351..68001 [+], 651302595410F pilin assembly  TrbC_F
25pECL46p082 (traN)67998..69806 [+], 1809302595411type IV secretion-like conjugative transfer system mating pair stabilization protein  TraN_F
26pECL46p083 (trbE)69830..70090 [+], 261302595412conjugal transfer protein 
27pECL46p085 (trbA)70842..71180 [+], 339302595413conjugal transfer protein 
28pECL46p086 (traQ)71307..71591 [+], 285302595414conjugal transfer protein  TraQ_F
29pECL46p087 (trbB)71578..72123 [+], 546302595415F pilin assembly periplasmic protein 
30pECL46p088 (trbJ)71972..72394 [+], 423302595416conjugal transfer protein 
31pECL46p089 (trbF)72342..72767 [+], 426302595417conjugal transfer protein 
32pECL46p091 (tnpA)73313..74035 [+], 723302595418IS26 transposase 
33pECL46p092 (tnpR)74361..75020 [-], 660302595419Tn3 resolvase 
 
flank Genes in the 5-Kb flanking regions if available, or non-essential genes in the T4SS gene cluster if any.

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Proteins        Genes
The information on structure of this T4ss

#NameImageResourceDetailReference
1TraM1DP3 PDB (1DP3)Solution structure of the DNA binding domain of the traM protein.(1) PubMed: 11258958
(1) Stockner T et al. (2001). Solution structure of the DNA-binding domain of TraM. Biochemistry. 40(11):3370-7. [PudMed:11258958] experimental
(1) Smet A; Van Nieuwerburgh F; Vandekerckhove TT; Martel A; Deforce D; Butaye P; Haesebrouck F (2010). Complete nucleotide sequence of CTX-M-15-plasmids from clinical Escherichia coli isolates: insertional events of transposons and insertion sequences. PLoS One. 5(6):e11202. [PudMed:20585456]