T4SS ID121
StrainEscherichia coli plasmid pSFO157 (NC_009602)
Repliconplasmid pSFO157 [Browse all T4SS(s) in this replicon]
ClassificationType IVA; Type F
sequence This T4SS has been mentioned in a genome sequencing report.

T4SS components

The information of T4SS components from NC_009602
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductComponent
1SF0157_p65 (psiB)83964..84398 [+], 435149930834plasmid SOS inhibition protein B 
2SF0157_p6684395..85114 [+], 720149930816plasmid SOS inhibition protein A 
3SF0157_p6786852..87673 [+], 822149930809w0067 
4SF0157_p6887969..88478 [-], 510149930830lytic transglycosylase  Orf169_F
5SF0157_p6988910..89293 [+], 384149930839conjugal transfer protein TraM  TraM_Faccesspry protein
6SF0157_p7089484..90131 [+], 648149930819conjugal transfer transcriptional regulator TraJ 
7SF0157_p7190525..90923 [+], 399149930837conjugal transfer pilin subunit TraA  TraA_F
8SF0157_p7290938..91249 [+], 312149930840conjugal transfer pilus assembly protein TraL  TraL_F
9SF0157_p7391271..91837 [+], 567149930824conjugal transfer pilus assembly protein TraE  TraE_F
10SF0157_p7491824..92552 [+], 729149930814conjugal transfer protein TraK  TraK_F
11SF0157_p7592773..93186 [+], 414149930806w0075 
12SF0157_p7693143..94294 [+], 1152149930805w0076 
13SF0157_p7794532..95209 [+], 678149930818w0077 
14SF0157_p7895779..96294 [+], 516149930828conjugal transfer protein TraV  TraV_F
15SF0157_p7996810..99437 [+], 2628149930801conjugal transfer ATP-binding protein TraC  TraC_F
16SF0157_p8099434..99820 [+], 387149930838conjugal transfer protein TrbI 
17SF0157_p8199817..100449 [+], 633149930821conjugal transfer pilus assembly protein TraW  TraW_F
18SF0157_p82100446..101438 [+], 993149930802conjugal transfer pilus assembly protein TraU  TraU_F
19SF0157_p83101444..102085 [+], 642149930820conjugal transfer pilus assembly protein TrbC  TrbC_F
20SF0157_p84102370..103890 [+], 1521149930804conjugal transfer mating pair stabilization protein TraN  TraN_F
21SF0157_p85104137..104910 [+], 774149930811conjugal pilus assembly protein TraF  TraF_F
22SF0157_p86104926..105273 [+], 348149930842conjugal transfer protein TrbA 
23SF0157_p87105663..106208 [+], 546149930827conjugal transfer protein TrbB 
24SF0157_p88106138..106500 [+], 363149930843conjugal transfer protein TrbJ 
25SF0157_p89106497..107873 [+], 1377149930800conjugal transfer pilus assembly protein TraH  TraH_F
26SF0157_p90107870..110692 [+], 2823149930799conjugal transfer mating pair stabilization protein TraG  TraG_F
27SF0157_p91110708..111193 [+], 486149930832w0091 
28SF0157_p92111242..111973 [+], 732149930813conjugal transfer surface exclusion protein TraT 
29SF0157_p93112226..114433 [+], 2208149930798conjugal transfer protein TraD  TraD_F
30SF0157_p94114433..115152 [+], 720149930815w0094 
31SF0157_p95115149..120419 [+], 5271149930845conjugal transfer nickase/helicase TraI  TraI_F
32SF0157_p96120439..121185 [+], 747149930812conjugal transfer pilus acetylation protein TraX  TraX_F
accesspry protein This T4SS contains information of accessory protein.
flank Genes in the 5-Kb flanking regions if available, or non-essential genes in the T4SS gene cluster if any.

Download FASTA format files
Proteins        Genes
The information on requirements for T4SS substrate-channel docking

Certain T4SS substrates require secretion chaperones for translocation. These chaperones often possess physical properties (small size of 15 kDa, acidic pI, and amphipathic helices) resembling those of chaperones associated with the type III secretion systems, a family of macromolecular translocation systems ancestrally related to bacterial flagella.

#Accessory protein(GI)motif(s)Substrate(s)FunctionReference
1TraM (149930839)NDTraIFor transfer initiation, traM mediates signalling between the relaxosome and transferosome.(1) PubMed: 22788760
1.Substrate(s): For the conjugation systems, the listed proteins are relaxases that bind a cognate T4CP and are delivered to recipient cells. For the effector translocator systems, the listed proteins are effectors that play a role in the infection processes of the bacterial pathogen.
2.motif(s):The motifs listed are required for substrate translocation. In some cases, the protein or its C-terminal fragment (CT) is sufficient to mediate translocation to target cells, as shown by fusion to a reporter protein such as Cre recombinase or adenylate cyclase. Amino acids (aa) at positions listed relative to the C-terminal fragment (subscript) are required for translocation, as shown by mutational analysis. ND, not determined. Parentheses indicate that the interaction between a protein substrate and a cognate T4CP has been experimentally shown.
3.Accessory protein: Accessory factors required for T4SS channel docking or translocation. The proposed function in mediating substrate-T4SS channel docking is shown in parentheses.PubMed:19946141

(1) Wong JJ; Lu J; Glover JN (2012). Relaxosome function and conjugation regulation in F-like plasmids - a structural biology perspective. Mol Microbiol. 85(4):602-17. [PudMed:22788760]
(1) Brunder W; Karch H; Schmidt H (2006). Complete sequence of the large virulence plasmid pSFO157 of the sorbitol-fermenting enterohemorrhagic Escherichia coli O157:H- strain 3072/96. Int J Med Microbiol. 296(7):467-74. [PudMed:16887390]