T4SS ID	121
Strain	Escherichia coli plasmid pSFO157 (NC_009602)
Replicon	plasmid pSFO157 [Browse all T4SS(s) in this replicon]
Accession	NC_009602
Location	87969..121185
Name	Tra_F
Function	conjugation
Classification	Type IVA; Type F
This T4SS has been mentioned in a genome sequencing report.

T4SS components

Component	TraA_F	TraB_F	TraC_F	TraD_F	TraE_F	TraF_F	TraG_F	TraH_F	TraI_F
Number	1	0	1	1	1	1	1	1	1
Component	TraK_F	TraL_F	TraM_F	TraN_F	TraQ_F	TraU_F	TraV_F	TraW_F	TraX_F
Number	1	1	1	1	0	1	1	1	1
Component	TraY_F	TrbC_F	Orf169_F
Number	0	1	1

The information of T4SS components from NC_009602
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Component
1	SF0157_p65 (psiB)	83964..84398 [+], 435	149930834	plasmid SOS inhibition protein B
2	SF0157_p66	84395..85114 [+], 720	149930816	plasmid SOS inhibition protein A
3	SF0157_p67	86852..87673 [+], 822	149930809	w0067
4	SF0157_p68	87969..88478 [-], 510	149930830	lytic transglycosylase	Orf169_F
5	SF0157_p69	88910..89293 [+], 384	149930839	conjugal transfer protein TraM	TraM_F
6	SF0157_p70	89484..90131 [+], 648	149930819	conjugal transfer transcriptional regulator TraJ
7	SF0157_p71	90525..90923 [+], 399	149930837	conjugal transfer pilin subunit TraA	TraA_F
8	SF0157_p72	90938..91249 [+], 312	149930840	conjugal transfer pilus assembly protein TraL	TraL_F
9	SF0157_p73	91271..91837 [+], 567	149930824	conjugal transfer pilus assembly protein TraE	TraE_F
10	SF0157_p74	91824..92552 [+], 729	149930814	conjugal transfer protein TraK	TraK_F
11	SF0157_p75	92773..93186 [+], 414	149930806	w0075
12	SF0157_p76	93143..94294 [+], 1152	149930805	w0076
13	SF0157_p77	94532..95209 [+], 678	149930818	w0077
14	SF0157_p78	95779..96294 [+], 516	149930828	conjugal transfer protein TraV	TraV_F
15	SF0157_p79	96810..99437 [+], 2628	149930801	conjugal transfer ATP-binding protein TraC	TraC_F
16	SF0157_p80	99434..99820 [+], 387	149930838	conjugal transfer protein TrbI
17	SF0157_p81	99817..100449 [+], 633	149930821	conjugal transfer pilus assembly protein TraW	TraW_F
18	SF0157_p82	100446..101438 [+], 993	149930802	conjugal transfer pilus assembly protein TraU	TraU_F
19	SF0157_p83	101444..102085 [+], 642	149930820	conjugal transfer pilus assembly protein TrbC	TrbC_F
20	SF0157_p84	102370..103890 [+], 1521	149930804	conjugal transfer mating pair stabilization protein TraN	TraN_F
21	SF0157_p85	104137..104910 [+], 774	149930811	conjugal pilus assembly protein TraF	TraF_F
22	SF0157_p86	104926..105273 [+], 348	149930842	conjugal transfer protein TrbA
23	SF0157_p87	105663..106208 [+], 546	149930827	conjugal transfer protein TrbB
24	SF0157_p88	106138..106500 [+], 363	149930843	conjugal transfer protein TrbJ
25	SF0157_p89	106497..107873 [+], 1377	149930800	conjugal transfer pilus assembly protein TraH	TraH_F
26	SF0157_p90	107870..110692 [+], 2823	149930799	conjugal transfer mating pair stabilization protein TraG	TraG_F
27	SF0157_p91	110708..111193 [+], 486	149930832	w0091
28	SF0157_p92	111242..111973 [+], 732	149930813	conjugal transfer surface exclusion protein TraT
29	SF0157_p93	112226..114433 [+], 2208	149930798	conjugal transfer protein TraD	TraD_F
30	SF0157_p94	114433..115152 [+], 720	149930815	w0094
31	SF0157_p95	115149..120419 [+], 5271	149930845	conjugal transfer nickase/helicase TraI	TraI_F
32	SF0157_p96	120439..121185 [+], 747	149930812	conjugal transfer pilus acetylation protein TraX	TraX_F

 This T4SS contains information of accessory protein.

Genes in the 5-Kb flanking regions if available, or non-essential genes in the T4SS gene cluster if any.

Download FASTA format files

Proteins        Genes

The information on requirements for T4SS substrate-channel docking

Certain T4SS substrates require secretion chaperones for translocation. These chaperones often possess physical properties (small size of 15 kDa, acidic pI, and amphipathic helices) resembling those of chaperones associated with the type III secretion systems, a family of macromolecular translocation systems ancestrally related to bacterial flagella.

#	Accessory protein(GI)	motif(s)	Substrate(s)	Function	Reference
1	TraM (149930839)	ND	TraI	For transfer initiation, traM mediates signalling between the relaxosome and transferosome.	(1) PubMed: 22788760

Tips:
1.Substrate(s): For the conjugation systems, the listed proteins are relaxases that bind a cognate T4CP and are delivered to recipient cells. For the effector translocator systems, the listed proteins are effectors that play a role in the infection processes of the bacterial pathogen.
2.motif(s):The motifs listed are required for substrate translocation. In some cases, the protein or its C-terminal fragment (CT) is sufficient to mediate translocation to target cells, as shown by fusion to a reporter protein such as Cre recombinase or adenylate cyclase. Amino acids (aa) at positions listed relative to the C-terminal fragment (subscript) are required for translocation, as shown by mutational analysis. ND, not determined. Parentheses indicate that the interaction between a protein substrate and a cognate T4CP has been experimentally shown.
3.Accessory protein: Accessory factors required for T4SS channel docking or translocation. The proposed function in mediating substrate-T4SS channel docking is shown in parentheses.PubMed:19946141

(1) Wong JJ; Lu J; Glover JN (2012). Relaxosome function and conjugation regulation in F-like plasmids - a structural biology perspective. Mol Microbiol. 85(4):602-17. [PudMed:22788760]

(1) Brunder W; Karch H; Schmidt H (2006). Complete sequence of the large virulence plasmid pSFO157 of the sorbitol-fermenting enterohemorrhagic Escherichia coli O157:H- strain 3072/96. Int J Med Microbiol. 296(7):467-74. [PudMed:16887390]