T4SS ID	120
Strain	Escherichia coli plasmid pEC_L8 (NC_014384)
Replicon	plasmid pEC_L8 [Browse all T4SS(s) in this replicon]
Accession	NC_014384
Location	45099..77556
Name	Tra_F
Function	conjugation
Classification	Type IVA; Type F
This T4SS has been mentioned in a genome sequencing report.

T4SS components

Component	TraA_F	TraB_F	TraC_F	TraD_F	TraE_F	TraF_F	TraG_F	TraH_F	TraI_F
Number	1	1	0	1	1	0	1	1	1
Component	TraK_F	TraL_F	TraM_F	TraN_F	TraQ_F	TraU_F	TraV_F	TraW_F	TraX_F
Number	1	1	1	1	1	1	0	1	1
Component	TraY_F	TrbC_F	Orf169_F
Number	1	1	1

The information of T4SS components from NC_014384
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Component
1	pECL8_p044 (psiB)	41063..41500 [+], 438	302595506	plasmid SOS inhibition protein B
2	pECL8_p045 (psiA)	41497..42216 [+], 720	302595507	plasmid SOS inhibition protein A
3	pECL8_p046 (mok)	42228..42458 [-], 231	302595508	modulator of Hok protein
4	pECL8_p048	43008..43232 [-], 225	302595509	hypothetical protein
5	pECL8_p049	43276..43509 [-], 234	302595510	hypothetical protein
6	pECL8_p050 (yubP)	44921..45073 [+], 153	302595511	hypothetical protein
7	pECL8_p051 (geneX)	45099..45776 [-], 678	302595512	X-polypeptide	Orf169_F
8	pECL8_p052 (traM)	46032..46415 [+], 384	302595513	mating signal	TraM_F
9	pECL8_p053 (traJ)	46549..47295 [+], 747	302595514	regulation protein
10	pECL8_p054 (traY)	47389..47616 [+], 228	302595515	OriT nicking	TraY_F
11	pECL8_p055 (traA)	47626..48012 [+], 387	302595516	F pilin subunit	TraA_F
12	pECL8_p056 (traL)	48014..48328 [+], 315	302595517	F pilin assembly	TraL_F
13	pECL8_p057 (traE)	48350..48916 [+], 567	302595518	F pilin assembly	TraE_F
14	pECL8_p058 (traK)	48882..49631 [+], 750	302595519	F pilin assembly	TraK_F
15	pECL8_p059 (traB)	49628..51058 [+], 1431	302595520	F pilin assembly	TraB_F
16	pECL8_p060 (traP)	51006..51632 [+], 627	302595521	conjugal transfer protein
17	pECL8_p061 (trbD)	51529..51939 [+], 411	302595522	conjugal transfer protein
18	pECL8_p062 (trbG)	51917..52183 [+], 267	302595523	conjugal transfer protein
19	pECL8_p064 (traR)	52830..53051 [+], 222	302595524	conjugal transfer protein
20	pECL8_p066 (trbI)	55820..56221 [+], 402	302595525	conjugal transfer protein
21	pECL8_p067 (traW)	56104..56850 [+], 747	302595526	F pilus assembly	TraW_F
22	pECL8_p068 (traU)	56820..57839 [+], 1020	302595527	F pilin assembly	TraU_F
23	pECL8_p069 (yfdA)	57863..58174 [+], 312	302595528	hypothetical protein
24	pECL8_p070 (trbC)	58171..58821 [+], 651	302595529	F pilin assembly	TrbC_F
25	pECL8_p071 (traN)	58818..60626 [+], 1809	302595530	type IV secretion-like conjugative transfer system mating pair stabilization protein	TraN_F
26	pECL8_p072 (trbE)	60650..60910 [+], 261	302595531	conjugal transfer protein
27	pECL8_p074 (trbA)	61662..62000 [+], 339	302595532	conjugative transfer protein
28	pECL8_p075 (traQ)	62127..62411 [+], 285	302595533	conjugal transfer protein	TraQ_F
29	pECL8_p076 (trbB)	62398..62943 [+], 546	302595534	F pilin assembly periplasmic protein
30	pECL8_p077 (trbJ)	62792..63214 [+], 423	302595535	conjugal transfer protein
31	pECL8_p078 (trbF)	63162..63587 [+], 426	302595536	conjugal transfer protein
32	pECL8_p079 (traH)	63568..64947 [+], 1380	302595537	F pilin assembly	TraH_F
33	pECL8_p080 (traG)	64944..67763 [+], 2820	302595538	type IV secretion-like conjugative transfer system protein	TraG_F
34	pECL8_p081 (traS)	67782..68279 [+], 498	302595539	conjugal transfer protein
35	pECL8_p082 (traT)	68161..69042 [+], 882	302595540	conjugal transfer surface exclusion protein
36	pECL8_p083 (traD)	69268..71520 [+], 2253	302595541	coupling protein	TraD_F
37	pECL8_p084 (traI)	71517..76790 [+], 5274	302595542	nickase/helicase	TraI_F
38	pECL8_p085 (traX)	76339..77556 [+], 1218	302595543	F pilin acetylation protein	TraX_F
39	pECL8_p086 (finO)	78554..79138 [+], 585	302595544	fertility inhibition protein
40	pECL8_p087 (yigA)	79228..79479 [+], 252	302595545	hypothetical protein
41	pECL8_p089 (hhA)	80201..80440 [+], 240	302595546	hypothetical protein
42	pECL8_p090 (yihA)	80478..81068 [+], 591	302595547	hypothetical protein
43	pECL8_p091 (repA2)	81308..81568 [+], 261	302595548	negative regulator of repA1 expression in FII replicon
44	pECL8_p092 (repA3)	81653..81847 [+], 195	302595549	regulator of repA1 expression in FII replicon

 This T4SS contains information of accessory protein.

Genes in the 5-Kb flanking regions if available, or non-essential genes in the T4SS gene cluster if any.

Download FASTA format files

Proteins        Genes

The information on requirements for T4SS substrate-channel docking

Certain T4SS substrates require secretion chaperones for translocation. These chaperones often possess physical properties (small size of 15 kDa, acidic pI, and amphipathic helices) resembling those of chaperones associated with the type III secretion systems, a family of macromolecular translocation systems ancestrally related to bacterial flagella.

#	Accessory protein(GI)	motif(s)	Substrate(s)	Function	Reference
1	TraM (302595513)	ND	TraI	For transfer initiation, traM mediates signalling between the relaxosome and transferosome.	(1) PubMed: 22788760

Tips:
1.Substrate(s): For the conjugation systems, the listed proteins are relaxases that bind a cognate T4CP and are delivered to recipient cells. For the effector translocator systems, the listed proteins are effectors that play a role in the infection processes of the bacterial pathogen.
2.motif(s):The motifs listed are required for substrate translocation. In some cases, the protein or its C-terminal fragment (CT) is sufficient to mediate translocation to target cells, as shown by fusion to a reporter protein such as Cre recombinase or adenylate cyclase. Amino acids (aa) at positions listed relative to the C-terminal fragment (subscript) are required for translocation, as shown by mutational analysis. ND, not determined. Parentheses indicate that the interaction between a protein substrate and a cognate T4CP has been experimentally shown.
3.Accessory protein: Accessory factors required for T4SS channel docking or translocation. The proposed function in mediating substrate-T4SS channel docking is shown in parentheses.PubMed:19946141

(1) Wong JJ; Lu J; Glover JN (2012). Relaxosome function and conjugation regulation in F-like plasmids - a structural biology perspective. Mol Microbiol. 85(4):602-17. [PudMed:22788760]

(1) Smet A; Van Nieuwerburgh F; Vandekerckhove TT; Martel A; Deforce D; Butaye P; Haesebrouck F (2010). Complete nucleotide sequence of CTX-M-15-plasmids from clinical Escherichia coli isolates: insertional events of transposons and insertion sequences. PLoS One. 5(6):e11202. [PudMed:20585456]