T4SS ID	108
Strain	Escherichia coli plasmid pEC14_114 (NC_013175)
Replicon	plasmid pEC14_114 [Browse all T4SS(s) in this replicon]
Accession	NC_013175
Location	76876..111301
Name	Tra_F
Function	conjugation
Classification	Type IVA; Type F
This T4SS has been mentioned in a genome sequencing report.

T4SS components

Component	TraA_F	TraB_F	TraC_F	TraD_F	TraE_F	TraF_F	TraG_F	TraH_F	TraI_F
Number	1	1	1	1	1	1	1	1	1
Component	TraK_F	TraL_F	TraM_F	TraN_F	TraQ_F	TraU_F	TraV_F	TraW_F	TraX_F
Number	1	1	1	1	1	1	1	1	1
Component	TraY_F	TrbC_F	Orf169_F
Number	0	1	1

The information of T4SS components from NC_013175
#	Locus tag (Gene)	Coordinates [+/-], size (bp)	Protein GI	Product	Component
1	pEC14_76 (psiB)	72855..73289 [+], 435	256855325	plasmid SOS inhibition protein B
2	pEC14_77 (psiA)	73286..74005 [+], 720	256855326	plasmid SOS inhibition protein A
3	pEC14_78	74676..74999 [-], 324	256855327	hypothetical protein
4	pEC14_79	75287..75580 [-], 294	256855328	hypothetical protein
5	pEC14_80	75675..76580 [+], 906	256855329	hypothetical protein
6	pEC14_81	76876..77466 [-], 591	256855330	lytic transglycosylase	Orf169_F
7	pEC14_82 (traM)	77809..78192 [+], 384	256855331	conjugal transfer protein TraM	TraM_F
8	pEC14_83 (traJ)	78386..79072 [+], 687	256855332	IncF plasmid conjugative transfer protein TraJ
9	pEC14_84 (traA)	79427..79786 [+], 360	256855333	conjugal transfer pilin subunit TraA	TraA_F
10	pEC14_85 (traL)	79801..80112 [+], 312	256855334	conjugal transfer pilus assembly protein TraL	TraL_F
11	pEC14_86 (traE)	80134..80700 [+], 567	256855335	conjugal transfer pilus assembly protein TraE	TraE_F
12	pEC14_87 (traK)	80687..81415 [+], 729	256855336	conjugal transfer protein TraK	TraK_F
13	pEC14_88 (traB)	81573..82841 [+], 1269	256855337	IncF plasmid conjugative transfer pilus assembly protein TraB	TraB_F
14	pEC14_89 (traP)	82831..83421 [+], 591	256855338	conjugal transfer protein TraP
15	pEC14_90 (trbD)	83408..83728 [+], 321	256855339	conjugal transfer protein TrbD
16	pEC14_91 (traV)	83970..84485 [+], 516	256855340	conjugal transfer protein TraV	TraV_F
17	pEC14_92	84792..85307 [+], 516	256855341	hypothetical protein
18	pEC14_93 (traC)	85972..88602 [+], 2631	256855342	conjugal transfer ATP-binding protein TraC	TraC_F
19	pEC14_94 (trbI)	88599..88985 [+], 387	256855343	conjugal transfer protein TrbI
20	pEC14_95 (traW)	88982..89614 [+], 633	256855344	conjugal transfer pilus assembly protein TraW	TraW_F
21	pEC14_96 (traU)	89611..90603 [+], 993	256855345	conjugal transfer pilus assembly protein TraU	TraU_F
22	pEC14_97	90633..90938 [+], 306	256855346	hypothetical protein
23	pEC14_98 (trbC)	90947..91585 [+], 639	256855347	conjugal transfer pilus assembly protein TrbC	TrbC_F
24	pEC14_99 (traN)	91582..93432 [+], 1851	256855348	conjugal transfer mating pair stabilization protein TraN	TraN_F
25	pEC14_100 (trbE)	93459..93716 [+], 258	256855349	conjugal transfer protein TrbE
26	pEC14_101 (traF)	93709..94452 [+], 744	256855350	conjugal pilus assembly protein TraF	TraF_F
27	pEC14_102 (trbA)	94466..94807 [+], 342	256855351	conjugal transfer protein TrbA
28	pEC14_103 (traQ)	95204..95488 [+], 285	256855352	conjugal transfer pilin chaperone TraQ	TraQ_F
29	pEC14_104 (trbB)	95475..96020 [+], 546	256855353	conjugal transfer protein TrbB
30	pEC14_105 (trbJ)	96010..96297 [+], 288	256855354	conjugal transfer protein TrbJ
31	pEC14_106 (trbF)	96278..96670 [+], 393	256855355	conjugal transfer protein TrbF
32	pEC14_107 (traH)	96657..98030 [+], 1374	256855356	conjugal transfer pilus assembly protein TraH	TraH_F
33	pEC14_108 (traG)	98027..100849 [+], 2823	256855357	conjugal transfer mating pair stabilization protein TraG	TraG_F
34	pEC14_109 (traS)	100904..101350 [+], 447	256855358	IncF plasmid conjugative transfer surface exclusion protein TraS
35	pEC14_110 (traT)	101399..102130 [+], 732	256855359	conjugal transfer surface exclusion protein TraT
36	pEC14_111	102333..103070 [+], 738	256855360	hypothetical protein
37	pEC14_112 (traD)	103121..105265 [+], 2145	256855361	conjugal transfer protein TraD	TraD_F
38	pEC14_113 (traI)	105265..110535 [+], 5271	256855362	conjugal transfer nickase/helicase TraI	TraI_F
39	pEC14_114 (traX)	110555..111301 [+], 747	256855363	conjugal transfer pilus acetylation protein TraX	TraX_F
40	pEC14_115	111360..112220 [+], 861	256855364	hypothetical protein
41	pEC14_116 (finO)	112323..112883 [+], 561	256855365	conjugal transfer fertility inhibition protein FinO
42	pEC14_117	113406..113930 [+], 525	256855366	putative regulatory protein

 This T4SS contains information of accessory protein.

Genes in the 5-Kb flanking regions if available, or non-essential genes in the T4SS gene cluster if any.

Download FASTA format files

Proteins        Genes

The information on requirements for T4SS substrate-channel docking

Certain T4SS substrates require secretion chaperones for translocation. These chaperones often possess physical properties (small size of 15 kDa, acidic pI, and amphipathic helices) resembling those of chaperones associated with the type III secretion systems, a family of macromolecular translocation systems ancestrally related to bacterial flagella.

#	Accessory protein(GI)	motif(s)	Substrate(s)	Function	Reference
1	TraM (256855331)	ND	TraI	For transfer initiation, traM mediates signalling between the relaxosome and transferosome.	(1) PubMed: 22788760

Tips:
1.Substrate(s): For the conjugation systems, the listed proteins are relaxases that bind a cognate T4CP and are delivered to recipient cells. For the effector translocator systems, the listed proteins are effectors that play a role in the infection processes of the bacterial pathogen.
2.motif(s):The motifs listed are required for substrate translocation. In some cases, the protein or its C-terminal fragment (CT) is sufficient to mediate translocation to target cells, as shown by fusion to a reporter protein such as Cre recombinase or adenylate cyclase. Amino acids (aa) at positions listed relative to the C-terminal fragment (subscript) are required for translocation, as shown by mutational analysis. ND, not determined. Parentheses indicate that the interaction between a protein substrate and a cognate T4CP has been experimentally shown.
3.Accessory protein: Accessory factors required for T4SS channel docking or translocation. The proposed function in mediating substrate-T4SS channel docking is shown in parentheses.PubMed:19946141

(1) Wong JJ; Lu J; Glover JN (2012). Relaxosome function and conjugation regulation in F-like plasmids - a structural biology perspective. Mol Microbiol. 85(4):602-17. [PudMed:22788760]

(1) DebRoy C; Sidhu MS; Sarker U; Jayarao BM; Stell AL; Bell NP; Johnson TJ (2010). Complete sequence of pEC14_114, a highly conserved IncFIB/FIIA plasmid associated with uropathogenic Escherichia coli cystitis strains. Plasmid. 63(1):53-60. [PudMed:19887083]