SecReT4
T4SS ID108
StrainEscherichia coli plasmid pEC14_114 (NC_013175)
Repliconplasmid pEC14_114 [Browse all T4SS(s) in this replicon]
AccessionNC_013175
Location76876..111301
NameTra_F
Functionconjugation
ClassificationType IVA; Type F
sequence This T4SS has been mentioned in a genome sequencing report.

T4SS components
ComponentTraA_FTraB_FTraC_FTraD_FTraE_FTraF_FTraG_FTraH_FTraI_F
Number111111111
ComponentTraK_FTraL_FTraM_FTraN_FTraQ_FTraU_FTraV_FTraW_FTraX_F
Number111111111
ComponentTraY_FTrbC_FOrf169_F
Number011

The information of T4SS components from NC_013175
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductComponent
1pEC14_76 (psiB)72855..73289 [+], 435256855325plasmid SOS inhibition protein B 
2pEC14_77 (psiA)73286..74005 [+], 720256855326plasmid SOS inhibition protein A 
3pEC14_7874676..74999 [-], 324256855327hypothetical protein 
4pEC14_7975287..75580 [-], 294256855328hypothetical protein 
5pEC14_8075675..76580 [+], 906256855329hypothetical protein 
6pEC14_8176876..77466 [-], 591256855330lytic transglycosylase  Orf169_F
7pEC14_82 (traM)77809..78192 [+], 384256855331conjugal transfer protein TraM  TraM_Faccesspry protein
8pEC14_83 (traJ)78386..79072 [+], 687256855332IncF plasmid conjugative transfer protein TraJ 
9pEC14_84 (traA)79427..79786 [+], 360256855333conjugal transfer pilin subunit TraA  TraA_F
10pEC14_85 (traL)79801..80112 [+], 312256855334conjugal transfer pilus assembly protein TraL  TraL_F
11pEC14_86 (traE)80134..80700 [+], 567256855335conjugal transfer pilus assembly protein TraE  TraE_F
12pEC14_87 (traK)80687..81415 [+], 729256855336conjugal transfer protein TraK  TraK_F
13pEC14_88 (traB)81573..82841 [+], 1269256855337IncF plasmid conjugative transfer pilus assembly protein TraB  TraB_F
14pEC14_89 (traP)82831..83421 [+], 591256855338conjugal transfer protein TraP 
15pEC14_90 (trbD)83408..83728 [+], 321256855339conjugal transfer protein TrbD 
16pEC14_91 (traV)83970..84485 [+], 516256855340conjugal transfer protein TraV  TraV_F
17pEC14_9284792..85307 [+], 516256855341hypothetical protein 
18pEC14_93 (traC)85972..88602 [+], 2631256855342conjugal transfer ATP-binding protein TraC  TraC_F
19pEC14_94 (trbI)88599..88985 [+], 387256855343conjugal transfer protein TrbI 
20pEC14_95 (traW)88982..89614 [+], 633256855344conjugal transfer pilus assembly protein TraW  TraW_F
21pEC14_96 (traU)89611..90603 [+], 993256855345conjugal transfer pilus assembly protein TraU  TraU_F
22pEC14_9790633..90938 [+], 306256855346hypothetical protein 
23pEC14_98 (trbC)90947..91585 [+], 639256855347conjugal transfer pilus assembly protein TrbC  TrbC_F
24pEC14_99 (traN)91582..93432 [+], 1851256855348conjugal transfer mating pair stabilization protein TraN  TraN_F
25pEC14_100 (trbE)93459..93716 [+], 258256855349conjugal transfer protein TrbE 
26pEC14_101 (traF)93709..94452 [+], 744256855350conjugal pilus assembly protein TraF  TraF_F
27pEC14_102 (trbA)94466..94807 [+], 342256855351conjugal transfer protein TrbA 
28pEC14_103 (traQ)95204..95488 [+], 285256855352conjugal transfer pilin chaperone TraQ  TraQ_F
29pEC14_104 (trbB)95475..96020 [+], 546256855353conjugal transfer protein TrbB 
30pEC14_105 (trbJ)96010..96297 [+], 288256855354conjugal transfer protein TrbJ 
31pEC14_106 (trbF)96278..96670 [+], 393256855355conjugal transfer protein TrbF 
32pEC14_107 (traH)96657..98030 [+], 1374256855356conjugal transfer pilus assembly protein TraH  TraH_F
33pEC14_108 (traG)98027..100849 [+], 2823256855357conjugal transfer mating pair stabilization protein TraG  TraG_F
34pEC14_109 (traS)100904..101350 [+], 447256855358IncF plasmid conjugative transfer surface exclusion protein TraS 
35pEC14_110 (traT)101399..102130 [+], 732256855359conjugal transfer surface exclusion protein TraT 
36pEC14_111102333..103070 [+], 738256855360hypothetical protein 
37pEC14_112 (traD)103121..105265 [+], 2145256855361conjugal transfer protein TraD  TraD_F
38pEC14_113 (traI)105265..110535 [+], 5271256855362conjugal transfer nickase/helicase TraI  TraI_F
39pEC14_114 (traX)110555..111301 [+], 747256855363conjugal transfer pilus acetylation protein TraX  TraX_F
40pEC14_115111360..112220 [+], 861256855364hypothetical protein 
41pEC14_116 (finO)112323..112883 [+], 561256855365conjugal transfer fertility inhibition protein FinO 
42pEC14_117113406..113930 [+], 525256855366putative regulatory protein 
 
accesspry protein This T4SS contains information of accessory protein.
flank Genes in the 5-Kb flanking regions if available, or non-essential genes in the T4SS gene cluster if any.

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Proteins        Genes
The information on requirements for T4SS substrate-channel docking

Certain T4SS substrates require secretion chaperones for translocation. These chaperones often possess physical properties (small size of 15 kDa, acidic pI, and amphipathic helices) resembling those of chaperones associated with the type III secretion systems, a family of macromolecular translocation systems ancestrally related to bacterial flagella.

#Accessory protein(GI)motif(s)Substrate(s)FunctionReference
1TraM (256855331)NDTraIFor transfer initiation, traM mediates signalling between the relaxosome and transferosome.(1) PubMed: 22788760
Tips:
1.Substrate(s): For the conjugation systems, the listed proteins are relaxases that bind a cognate T4CP and are delivered to recipient cells. For the effector translocator systems, the listed proteins are effectors that play a role in the infection processes of the bacterial pathogen.
2.motif(s):The motifs listed are required for substrate translocation. In some cases, the protein or its C-terminal fragment (CT) is sufficient to mediate translocation to target cells, as shown by fusion to a reporter protein such as Cre recombinase or adenylate cyclase. Amino acids (aa) at positions listed relative to the C-terminal fragment (subscript) are required for translocation, as shown by mutational analysis. ND, not determined. Parentheses indicate that the interaction between a protein substrate and a cognate T4CP has been experimentally shown.
3.Accessory protein: Accessory factors required for T4SS channel docking or translocation. The proposed function in mediating substrate-T4SS channel docking is shown in parentheses.PubMed:19946141

(1) Wong JJ; Lu J; Glover JN (2012). Relaxosome function and conjugation regulation in F-like plasmids - a structural biology perspective. Mol Microbiol. 85(4):602-17. [PudMed:22788760]
(1) DebRoy C; Sidhu MS; Sarker U; Jayarao BM; Stell AL; Bell NP; Johnson TJ (2010). Complete sequence of pEC14_114, a highly conserved IncFIB/FIIA plasmid associated with uropathogenic Escherichia coli cystitis strains. Plasmid. 63(1):53-60. [PudMed:19887083]