SecReT4
T4SS ID104
StrainEscherichia coli plasmid p1658/97 (NC_004998)
Repliconplasmid p1658/97 [Browse all T4SS(s) in this replicon]
AccessionNC_004998
Location1541..34695
NameTra_F
Functionconjugation
ClassificationType IVA; Type F
sequence This T4SS has been mentioned in a genome sequencing report.

T4SS components
ComponentTraA_FTraB_FTraC_FTraD_FTraE_FTraF_FTraG_FTraH_FTraI_F
Number111111111
ComponentTraK_FTraL_FTraM_FTraN_FTraQ_FTraU_FTraV_FTraW_FTraX_F
Number111111111
ComponentTraY_FTrbC_FOrf169_F
Number111

The information of T4SS components from NC_004998
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductComponent
1p165897_001 (orf1)14..301 [+], 28832469974hypothetical protein 
2p165897_002 (orf2)423..1244 [+], 82232469975hypothetical protein 
3p165897_003 (orf3)1541..2050 [-], 51032469976lytic transglycosylase  Orf169_F
4p165897_004 (traM)2464..2847 [+], 38432469977conjugal transfer protein TraM  TraM_Faccesspry protein
5p165897_005 (traJ)3034..3723 [+], 69032469978conjugal transfer transcriptional regulator TraJ 
6p165897_006 (traY)3822..4217 [+], 39632469979conjugal transfer protein TraY  TraY_F
7p165897_007 (traA)4250..4615 [+], 36632469980conjugal transfer pilin subunit TraA  TraA_F
8p165897_008 (traL)4630..4941 [+], 31232469981conjugal transfer pilus assembly protein TraL  TraL_F
9p165897_009 (traE)4963..5529 [+], 56732469982conjugal transfer pilus assembly protein TraE  TraE_F
10p165897_010 (traK)5516..6244 [+], 72932469983conjugal transfer protein TraK  TraK_F
11p165897_011 (traB)6244..7671 [+], 142832469984conjugal transfer pilus assembly protein TraB  TraB_F
12p165897_012 (traP)7661..8251 [+], 59132469985conjugal transfer protein TraP 
13p165897_013 (trbD)8238..8435 [+], 19832469986conjugal transfer protein TrbD 
14p165897_014 (trbG)8447..8698 [+], 25232469987conjugal transfer protein TrbG 
15p165897_015 (traV)8695..9210 [+], 51632469988conjugal transfer protein TraV  TraV_F
16p165897_016 (traR)9345..9566 [+], 22232469989conjugal transfer protein TraR 
17p165897_017 (traC)9726..12353 [+], 262832469990conjugal transfer ATP-binding protein TraC  TraC_F
18p165897_018 (trbI)12350..12736 [+], 38732470111conjugal transfer protein TrbI 
19p165897_019 (traW)12733..13365 [+], 63332469991conjugal transfer pilus assembly protein TraW  TraW_F
20p165897_020 (traU)13362..14354 [+], 99332469992conjugal transfer pilus assembly protein TraU  TraU_F
21p165897_021 (trbC)14363..15001 [+], 63932469993conjugal transfer pilus assembly protein TrbC  TrbC_F
22p165897_022 (traN)14998..16806 [+], 180932469994conjugal transfer mating pair stabilization protein TraN  TraN_F
23p165897_023 (trbE)16830..17090 [+], 26132469995conjugal transfer protein TrbE 
24p165897_024 (traF)17053..17826 [+], 77432469996conjugal pilus assembly protein TraF  TraF_F
25p165897_025 (trbA)17842..18189 [+], 34832469997conjugal transfer protein TrbA 
26p165897_026 (artA)18191..18505 [-], 31532469998hypothetical protein 
27p165897_027 (traQ)18586..18870 [+], 28532469999conjugal transfer pilin chaperone TraQ  TraQ_F
28p165897_028 (trbB)18857..19402 [+], 54632470000conjugal transfer protein TrbB 
29p165897_029 (trbJ)19332..19673 [+], 34232470001conjugal transfer protein TrbJ 
30p165897_030 (trbF)19660..19965 [+], 30632470002conjugal transfer protein TrbF 
31p165897_031 (traH)20038..21414 [+], 137732470003conjugal transfer pilus assembly protein TraH  TraH_F
32p165897_032 (traG)21411..24227 [+], 281732470004conjugal transfer mating pair stabilization protein TraG  TraG_F
33p165897_033 (traS)24260..24781 [+], 52232470005conjugal transfer entry exclusion protein TraS 
34p165897_034 (traT)24803..25537 [+], 73532470006conjugal transfer surface exclusion protein TraT 
35p165897_035 (traD)25790..27943 [+], 215432470007conjugal transfer protein TraD  TraD_F
36p165897_036 (trbH)27943..28662 [+], 72032470008TrbH 
37p165897_037 (orf5)27952..28350 [-], 39932470009plasmid maintenance protein 
38p165897_038 (orf6)28350..28577 [-], 22832470010hypothetical protein 
39p165897_039 (traI)28659..33929 [+], 527132470011conjugal transfer nickase/helicase TraI  TraI_F
40p165897_040 (traX)33949..34695 [+], 74732470012conjugal transfer pilus acetylation protein TraX  TraX_F
41p165897_041 (finO)34750..35310 [+], 56132470013conjugal transfer fertility inhibition protein FinO 
42p165897_042 (orf7)35452..35706 [+], 25532470014hypothetical protein 
43p165897_043 (orfA)35903..36364 [+], 46232470015hypothetical protein 
44p165897_044 (orf8)36410..36619 [+], 21032470016putative regulator 
45p165897_045 (orf9)36787..37230 [+], 44432470017hypothetical protein 
46p165897_046 (repA6)37955..38029 [+], 7532470112leader peptide RepL 
47p165897_047 (repA1)38022..38879 [+], 85832470018replication protein 
48p165897_048 (repA4)39243..39449 [+], 20732470113hypothetical protein 
 
accesspry protein This T4SS contains information of accessory protein.
flank Genes in the 5-Kb flanking regions if available, or non-essential genes in the T4SS gene cluster if any.

Download FASTA format files
Proteins        Genes
The information on requirements for T4SS substrate-channel docking

Certain T4SS substrates require secretion chaperones for translocation. These chaperones often possess physical properties (small size of 15 kDa, acidic pI, and amphipathic helices) resembling those of chaperones associated with the type III secretion systems, a family of macromolecular translocation systems ancestrally related to bacterial flagella.

#Accessory protein(GI)motif(s)Substrate(s)FunctionReference
1TraM (32469977)NDTraIFor transfer initiation, traM mediates signalling between the relaxosome and transferosome.(1) PubMed: 22788760
Tips:
1.Substrate(s): For the conjugation systems, the listed proteins are relaxases that bind a cognate T4CP and are delivered to recipient cells. For the effector translocator systems, the listed proteins are effectors that play a role in the infection processes of the bacterial pathogen.
2.motif(s):The motifs listed are required for substrate translocation. In some cases, the protein or its C-terminal fragment (CT) is sufficient to mediate translocation to target cells, as shown by fusion to a reporter protein such as Cre recombinase or adenylate cyclase. Amino acids (aa) at positions listed relative to the C-terminal fragment (subscript) are required for translocation, as shown by mutational analysis. ND, not determined. Parentheses indicate that the interaction between a protein substrate and a cognate T4CP has been experimentally shown.
3.Accessory protein: Accessory factors required for T4SS channel docking or translocation. The proposed function in mediating substrate-T4SS channel docking is shown in parentheses.PubMed:19946141

(1) Wong JJ; Lu J; Glover JN (2012). Relaxosome function and conjugation regulation in F-like plasmids - a structural biology perspective. Mol Microbiol. 85(4):602-17. [PudMed:22788760]
(1) Zienkiewicz M; Kern-Zdanowicz I; Golebiewski M; Zylinska J; Mieczkowski P; Gniadkowski M; Bardowski J; Ceglowski P (2007). Mosaic structure of p1658/97, a 125-kilobase plasmid harboring an active amplicon with the extended-spectrum beta-lactamase gene blaSHV-5. Antimicrob Agents Chemother. 51(4):1164-71. [PudMed:17220406]
 

Genomics & Bioinformatics Group in MML, SJTU