SecReT4
T4SS ID102
StrainEscherichia coli plasmid pAPEC-O2-R (NC_006671)
Repliconplasmid pAPEC-O2-R [Browse all T4SS(s) in this replicon]
AccessionNC_006671
Location7540..40351
NameTra_F
Functionconjugation
ClassificationType IVA; Type F
sequence This T4SS has been mentioned in a genome sequencing report.

T4SS components
ComponentTraA_FTraB_FTraC_FTraD_FTraE_FTraF_FTraG_FTraH_FTraI_F
Number111111111
ComponentTraK_FTraL_FTraM_FTraN_FTraQ_FTraU_FTraV_FTraW_FTraX_F
Number111111111
ComponentTraY_FTrbC_FOrf169_F
Number111

The information of T4SS components from NC_006671
#Locus tag (Gene)Coordinates [+/-], size (bp)Protein GIProductComponent
1O2R_94028..4618 [-], 59158000398hypothetical protein 
2O2R_10 (hha)4656..4865 [-], 21058000397Hha protein 
3O2R_11 (yigB)4911..5435 [-], 52558000396YigB 
4O2R_125571..5813 [-], 24358000395hypothetical protein 
5O2R_13 (finO)5958..6518 [-], 56158000394conjugal transfer fertility inhibition protein FinO 
6O2R_146621..7481 [-], 86158000393hypothetical protein 
7O2R_15 (traX)7540..8286 [-], 74758000392conjugal transfer pilus acetylation protein TraX  TraX_F
8O2R_16 (traI)8306..13576 [-], 527158000391conjugal transfer nickase/helicase TraI  TraI_F
9O2R_17 (traD)13576..15726 [-], 215158000390conjugal transfer protein TraD  TraD_F
10O2R_18 (traT)16027..16803 [-], 77758000389conjugal transfer surface exclusion protein TraT 
11O2R_20 (traG)17308..20130 [-], 282358000388conjugal transfer mating pair stabilization protein TraG  TraG_F
12O2R_21 (traH)20127..21503 [-], 137758000387conjugal transfer pilus assembly protein TraH  TraH_F
13O2R_22 (trbJ)21500..21862 [-], 363228861735conjugal transfer protein TrbJ 
14O2R_2321792..22337 [-], 54658000385conjugal transfer protein TrbB 
15O2R_24 (traQ)22324..22608 [-], 28558000384conjugal transfer pilin chaperone TraQ  TraQ_F
16O2R_25 (trbA)22727..23074 [-], 34858000383conjugal transfer protein TrbA 
17O2R_26 (traF)23090..23833 [-], 74458000382conjugal pilus assembly protein TraF  TraF_F
18O2R_2723826..24083 [-], 25858000381conjugal transfer protein TrbE 
19O2R_28 (traN)24110..25918 [-], 180958000380conjugal transfer mating pair stabilization protein TraN  TraN_F
20O2R_29 (trbC)25915..26517 [-], 60358000379conjugal transfer pilus assembly protein TrbC  TrbC_F
21O2R_30 (traU)26562..27554 [-], 99358000378conjugal transfer pilus assembly protein TraU  TraU_F
22O2R_31 (traW)27551..28183 [-], 633228861734conjugal transfer pilus assembly protein TraW  TraW_F
23O2R_32 (trbI)28180..28566 [-], 38758000376conjugal transfer protein TrbI 
24O2R_33 (traC)28563..31193 [-], 263158000375conjugal transfer ATP-binding protein TraC  TraC_F
25O2R_3431319..31681 [-], 36358000374hypothetical protein 
26O2R_3531709..31924 [-], 21658000373hypothetical protein 
27O2R_3632004..32477 [-], 47458000372hypothetical protein 
28O2R_37 (traR)32470..32682 [-], 21358000371conjugal transfer protein TraR 
29O2R_38 (traV)32761..33276 [-], 51658000370conjugal transfer protein TraV  TraV_F
30O2R_39 (trbG)33273..33524 [-], 25258000369conjugal transfer protein TrbG 
31O2R_40 (trbD)33521..33838 [-], 31858000368conjugal transfer protein TrbD 
32O2R_41 (traP)33835..34407 [-], 57358000367conjugal transfer protein TraP 
33O2R_42 (traB)34397..35824 [-], 142858000366conjugal transfer pilus assembly protein TraB  TraB_F
34O2R_43 (traK)35824..36552 [-], 72958000365conjugal transfer protein TraK  TraK_F
35O2R_44 (traE)36539..37105 [-], 56758000364conjugal transfer pilus assembly protein TraE  TraE_F
36O2R_45 (traL)37127..37438 [-], 31258000363conjugal transfer pilus assembly protein TraL  TraL_F
37O2R_46 (traA)37453..37812 [-], 36058000362conjugal transfer pilin subunit TraA  TraA_F
38O2R_47 (traY)37846..38166 [-], 32158000361conjugal transfer protein TraY  TraY_F
39O2R_48 (traJ)38167..38853 [-], 68758000360hypothetical protein 
40O2R_49 (traM)39044..39427 [-], 38458000359conjugal transfer protein TraM  TraM_Faccesspry protein
41O2R_5039758..40351 [+], 59458000358lytic transglycosylase  Orf169_F
42O2R_5140648..41469 [-], 82258000357hypothetical protein 
43O2R_5241650..41922 [+], 27358000356hypothetical protein 
44O2R_5341902..42108 [-], 20758000355hypothetical protein 
45O2R_5442144..42356 [-], 21358000354hypothetical protein 
46O2R_5542279..42512 [+], 23458000353hypothetical protein 
47O2R_56 (hok)42798..42956 [-], 15958000352small toxic polypeptide 
48O2R_58 (psiA)43236..43955 [-], 72058000351plasmid SOS inhibition protein A 
49O2R_59 (psiB)43952..44386 [-], 43558000350plasmid SOS inhibition protein B 
 
accesspry protein This T4SS contains information of accessory protein.
flank Genes in the 5-Kb flanking regions if available, or non-essential genes in the T4SS gene cluster if any.

Download FASTA format files
Proteins        Genes
The information on requirements for T4SS substrate-channel docking

Certain T4SS substrates require secretion chaperones for translocation. These chaperones often possess physical properties (small size of 15 kDa, acidic pI, and amphipathic helices) resembling those of chaperones associated with the type III secretion systems, a family of macromolecular translocation systems ancestrally related to bacterial flagella.

#Accessory protein(GI)motif(s)Substrate(s)FunctionReference
1TraM (58000359)NDTraIFor transfer initiation, traM mediates signalling between the relaxosome and transferosome.(1) PubMed: 22788760
Tips:
1.Substrate(s): For the conjugation systems, the listed proteins are relaxases that bind a cognate T4CP and are delivered to recipient cells. For the effector translocator systems, the listed proteins are effectors that play a role in the infection processes of the bacterial pathogen.
2.motif(s):The motifs listed are required for substrate translocation. In some cases, the protein or its C-terminal fragment (CT) is sufficient to mediate translocation to target cells, as shown by fusion to a reporter protein such as Cre recombinase or adenylate cyclase. Amino acids (aa) at positions listed relative to the C-terminal fragment (subscript) are required for translocation, as shown by mutational analysis. ND, not determined. Parentheses indicate that the interaction between a protein substrate and a cognate T4CP has been experimentally shown.
3.Accessory protein: Accessory factors required for T4SS channel docking or translocation. The proposed function in mediating substrate-T4SS channel docking is shown in parentheses.PubMed:19946141

(1) Wong JJ; Lu J; Glover JN (2012). Relaxosome function and conjugation regulation in F-like plasmids - a structural biology perspective. Mol Microbiol. 85(4):602-17. [PudMed:22788760]
(1) Johnson TJ; Siek KE; Johnson SJ; Nolan LK (2005). DNA sequence and comparative genomics of pAPEC-O2-R, an avian pathogenic Escherichia coli transmissible R plasmid. Antimicrob Agents Chemother. 49(11):4681-8. [PudMed:16251312]
 

Genomics & Bioinformatics Group in MML, SJTU