Detailed information NTG0998276

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: EL020_RS08495
• Genome accession: NZ_LR134203
• Coordinates: 1669260..1671650 (-)
• Length: 796 a.a.
• NCBI ID: WP_058833213.1
• Uniprot ID: -
• Organism: Streptococcus lutetiensis strain NCTC11436
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 1664260..1676650

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EL020_RS08475 (NCTC11436_01812)	stkP/pknB	1664263..1666176 (-)	1914	WP_126402640.1	Stk1 family PASTA domain-containing Ser/Thr kinase	Regulator
EL020_RS08480 (NCTC11436_01813)	-	1666176..1666913 (-)	738	WP_043894973.1	Stp1/IreP family PP2C-type Ser/Thr phosphatase	-
EL020_RS08485 (NCTC11436_01814)	rsmB	1666951..1668273 (-)	1323	WP_020916309.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
EL020_RS08490 (NCTC11436_01815)	fmt	1668263..1669198 (-)	936	WP_020916308.1	methionyl-tRNA formyltransferase	-
EL020_RS08495 (NCTC11436_01816)	priA	1669260..1671650 (-)	2391	WP_058833213.1	primosomal protein N'	Machinery gene
EL020_RS08500 (NCTC11436_01817)	rpoZ	1671784..1672098 (-)	315	WP_014334364.1	DNA-directed RNA polymerase subunit omega	-
EL020_RS08505 (NCTC11436_01818)	gmk	1672122..1672739 (-)	618	WP_020916306.1	guanylate kinase	-
EL020_RS08510 (NCTC11436_01819)	-	1672900..1674507 (-)	1608	WP_058833214.1	ribonuclease Y	-
EL020_RS08515 (NCTC11436_01820)	-	1674657..1675142 (+)	486	WP_043894971.1	S-ribosylhomocysteine lyase	-
EL020_RS08520 (NCTC11436_01821)	-	1675230..1675994 (-)	765	WP_020916303.1	(S)-acetoin forming diacetyl reductase	-

Sequence

Protein

• Length: 796 a.a.
• Molecular weight: 91171.70 Da
• Isoelectric Point: 7.5079

>NTDB_id=998276 EL020_RS08495 WP_058833213.1 1669260..1671650(-) (priA) [Streptococcus lutetiensis strain NCTC11436]
MTKIAQVIVDVPLMQTDKPFSYLVPEDMQNQVMLGSRVHVPFGHGNRLLQGFVVGFSESLDSDISELKAISEVLDFEPVL
NEEQLTLADQMRHTVFSYKISILKSMIPNLLNSTYDKRLMPTDKLSDEERLALFGDKDSRLHSNLSDEEAKKVARLVQAE
KISVDYLAKDKKNIKTEKFYQVASEQLAQAEISARAKKRLMLRDYLLEHPETGKLVDLYQLFSRDVVKFFVENHLLTILE
KEKKRSDAYFDVATTDFLELNAEQAAVVEKVTSQIGKESKPFLLEGVTGSGKTEVYLHIIDKVLKLSKTAIVLVPEISLT
PQMTNRFISRFGKQVAIMHSALSDGEKFDEWRKIKSSQARVVVGARSAIFVPMENIGAIIIDEEHEATYKQESNPRYHAR
DVALLRAKYHKAVLLMGSATPSIESRARASRNVYQFLQLTHRANPLAKIPKVDIVDFRDYVGQQEVSNYTPHLLEKIADR
LEKHEQVVLMLNRRGYSSFVMCRDCGYVDECPNCDISLTLHMDTKTMNCHYCGFEKAIPHTCPNCHSRSIRYYGTGTQKA
YDELSEVFPQARILRMDVDTTRQKGAHQRILDKFGNHEADILLGTQMIAKGLDFPNVTLVGVLNADTSLNLPDFRSSERT
FQLLTQVAGRAGRAEKEGEVLIQTYNPQHYAIKLAQKQDYEAFYAYEMGIRKQLSYPPYYFTVGLTLSHKEEQTVVRKSF
ELLQILRQQLSDKIKILGPTPKPIARTHNLYHYQIIVKYRFEDHLESVLNQILDMTQLPENKYLRLVIDHEPQNFM

Nucleotide

• Length: 2391 bp

>NTDB_id=998276 EL020_RS08495 WP_058833213.1 1669260..1671650(-) (priA) [Streptococcus lutetiensis strain NCTC11436]
ATGACAAAAATCGCTCAGGTCATTGTTGATGTGCCATTAATGCAGACCGATAAGCCTTTCTCATATCTCGTGCCAGAAGA
CATGCAAAATCAAGTGATGTTAGGTTCACGGGTGCATGTTCCTTTTGGTCACGGCAATCGTCTTTTGCAGGGATTTGTTG
TCGGCTTTTCTGAAAGCTTAGACAGTGATATCAGTGAGCTTAAGGCTATTAGCGAGGTATTGGATTTTGAACCAGTTTTG
AATGAAGAACAGCTTACTTTAGCAGATCAAATGCGTCATACTGTTTTTTCTTATAAGATTTCAATCTTGAAATCAATGAT
TCCTAATTTGCTCAATTCAACATACGATAAACGTTTGATGCCGACAGATAAGCTGTCTGATGAAGAACGCTTAGCTTTAT
TTGGTGATAAAGATAGCAGGCTTCACTCAAATTTATCTGATGAAGAAGCTAAAAAAGTTGCCAGGTTGGTTCAAGCAGAA
AAAATTTCGGTTGATTATCTTGCTAAAGATAAGAAAAATATCAAAACTGAGAAATTCTATCAGGTTGCTTCTGAACAGTT
AGCTCAAGCAGAGATTTCAGCAAGAGCTAAGAAGCGTTTGATGTTACGTGATTACTTGCTTGAGCATCCTGAAACAGGAA
AATTGGTAGACTTGTACCAGCTCTTTTCACGTGATGTTGTCAAATTCTTTGTGGAAAATCATCTGCTAACCATTTTAGAA
AAAGAGAAGAAACGTTCGGATGCTTATTTTGACGTTGCAACGACGGATTTTCTTGAGTTAAATGCAGAACAAGCTGCTGT
GGTTGAAAAGGTGACTAGTCAAATTGGCAAAGAATCTAAACCTTTTTTGTTAGAAGGAGTGACTGGCTCAGGTAAGACTG
AAGTGTATTTGCACATTATTGATAAAGTCTTAAAACTTAGTAAAACAGCGATTGTTTTGGTGCCTGAGATTTCTTTGACG
CCTCAGATGACTAACCGCTTTATTTCACGTTTTGGTAAGCAAGTGGCAATTATGCACTCGGCTTTGTCTGATGGTGAAAA
ATTTGATGAGTGGCGAAAGATAAAATCTAGTCAAGCTCGCGTTGTCGTAGGTGCTAGATCTGCTATTTTTGTTCCAATGG
AAAATATTGGCGCAATTATCATCGACGAAGAGCATGAGGCAACTTACAAGCAAGAATCGAATCCGCGTTACCATGCGCGT
GATGTGGCTTTGCTTCGTGCAAAATATCATAAGGCCGTTTTGTTAATGGGGTCTGCAACGCCAAGCATTGAGAGCCGTGC
TCGTGCTAGTCGTAATGTTTATCAGTTCTTACAATTGACTCACCGTGCCAATCCTTTGGCTAAGATTCCAAAAGTTGATA
TTGTTGATTTTCGTGATTACGTTGGTCAGCAGGAAGTCAGCAACTATACCCCTCATCTACTGGAAAAAATTGCAGATCGT
TTAGAAAAACATGAGCAAGTTGTCCTTATGTTAAATCGTCGCGGCTATTCAAGTTTTGTCATGTGCCGTGATTGTGGCTA
TGTGGATGAGTGCCCAAATTGTGACATTTCCTTGACCCTTCATATGGATACCAAGACCATGAATTGTCATTACTGTGGTT
TTGAAAAAGCTATTCCACATACTTGCCCAAATTGTCATAGTCGCAGCATCCGTTATTACGGGACTGGGACTCAGAAAGCT
TATGATGAATTGTCGGAAGTTTTCCCACAAGCTCGTATCTTGCGAATGGATGTCGATACCACACGACAAAAAGGAGCGCA
TCAGCGAATTCTTGATAAATTTGGAAATCATGAAGCTGATATTTTGTTAGGAACACAGATGATTGCTAAGGGACTTGATT
TTCCAAATGTTACCTTAGTAGGGGTTTTAAATGCTGATACATCACTAAATTTGCCAGATTTTAGATCGTCTGAGCGAACA
TTTCAATTGTTGACTCAGGTTGCAGGTCGTGCAGGTCGTGCAGAAAAAGAAGGTGAGGTCTTAATTCAAACCTACAATCC
ACAGCATTATGCCATCAAGTTAGCTCAAAAGCAAGATTATGAAGCTTTTTATGCTTATGAGATGGGGATTCGTAAGCAAT
TATCTTATCCACCATATTATTTTACGGTTGGCTTAACCTTGTCACATAAGGAGGAGCAGACCGTTGTGCGAAAGTCCTTT
GAATTGCTTCAAATTTTGCGTCAGCAGTTATCTGACAAAATTAAAATTCTCGGTCCAACACCTAAGCCGATTGCCAGAAC
GCATAATCTTTATCATTATCAAATTATTGTTAAATATCGCTTTGAGGACCATTTAGAAAGTGTCTTGAATCAGATTTTAG
ACATGACTCAATTACCTGAAAATAAGTACTTGCGACTGGTAATAGACCATGAGCCACAAAATTTTATGTAG

Domains

Predicted by InterProScan.

• Primosomal protein N C-terminal domain (PriA_C) (698-792)

• Type III restriction enzyme, res subunit (ResIII) (258-422)

• Helicase conserved C-terminal domain (Helicase_C) (571-654)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (6-108)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (511-536)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Bacillus subtilis subsp. subtilis str. 168	48.084	100	0.489