Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   AAVB50_RS17945 Genome accession   NZ_CP154923
Coordinates   3352142..3355642 (-) Length   1166 a.a.
NCBI ID   WP_345806468.1    Uniprot ID   -
Organism   Bacillus subtilis strain FUA2233     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3347142..3360642
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAVB50_RS17935 (AAVB50_17930) sbcD 3347210..3348385 (-) 1176 WP_151262118.1 exonuclease subunit SbcD -
  AAVB50_RS17940 (AAVB50_17935) addA 3348457..3352155 (-) 3699 WP_345806467.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  AAVB50_RS17945 (AAVB50_17940) addB 3352142..3355642 (-) 3501 WP_345806468.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  AAVB50_RS17950 (AAVB50_17945) yhjR 3355826..3356263 (+) 438 WP_003245806.1 ferritin family protein -
  AAVB50_RS17955 (AAVB50_17950) yhjQ 3356381..3356707 (+) 327 WP_069322503.1 four-helix bundle copper-binding protein -
  AAVB50_RS17960 (AAVB50_17955) yhjP 3356737..3358464 (-) 1728 WP_345806469.1 SgrR family transcriptional regulator -
  AAVB50_RS17965 (AAVB50_17960) yhjO 3358578..3359783 (+) 1206 WP_029317581.1 MFS transporter -

Sequence

Protein


Download         Length: 1166 a.a.        Molecular weight: 134673.70 Da        Isoelectric Point: 5.5770

>NTDB_id=995561 AAVB50_RS17945 WP_345806468.1 3352142..3355642(-) (addB) [Bacillus subtilis strain FUA2233]
MGAEFLVGRSGSGKTKLIINSIQDELRRAPFGKPIIFLVPDQMTFLMEYELAKTPDMGGMIRAQVFSFSRLAWRVLQHTG
GMSRPFLTSTGVQMLLRKLIEEHKQEFKVYQKASDKSGFTAQVERMLTEFKRYCLEPEDIRRMAESGTASEYRGERVLSE
KLHDLSILYQQMEKSLADQYLHSEDYLTLLAEHIPLAEDIKGAHIYVDGFYQFTPQEFRVLEQLMVHAEHITFSLTADKP
SYEREPHELELFRMTGKTYYRLHQKAKELNLDITYKELSGTERHTKTPELAHLEAQYEARPAIPYSEKQEALTVMQAANR
RAELEGIAREIHALVREKGYRYKDVAILARQPEDYKDMVKEVFADYEIPYFIDGKASMLNHPLIEFIRSSLDVLKGNWRY
EAVFRCVKTELLFPLNEPKAKVREQVDQLENYCIAYGIKGDRWTKGDRFQYRRFVSLDDDFAQTDQEIEMENMLNDTRDW
IVPPLFQLQKRMKKAKTVQEKAEALYRYLEETDVPLKLDQERQRAEDDGRIIEAQQHQQAWDAVIQLLEEFVEMMGDDEI
SLDLFQQMIEAGAESLTFSLIPPALDQVFVGNMDLSRMYGTSCTFVLGANDGVLPARPDENGVLSDDDREWLKTIGVELS
SGGRERLLEEHFLIYMAFSSPSDRLYVSYPIADAEGKTLLPSMIVKRLEELFPHHKERLLTNEPEQVSDEEQLMYVVNKS
VAQSFTASQLRLWTREYDISDVWWSTYNVLMSEQDRLQSKKLFSSLFFRNEVKQLERSVSRQLYGERIQGSVSRMETFNA
CPFSHFASHGLHLKERQFFKLEAPDIGQLFHSSLKLISDRLREQKLDWRDLTKEQCELFSYDAVERLAPKLQKEILLSSN
RHYYVKEKLQKIVTRVSGILSEHAKASGFVPIGLELGFGGKGPLPPLTFQLKNGCTMELVGRIDRVDKAESSKGLLLRIV
DYKSSDKGLDLAEVYYGLALQMLTYLDLSITHSADWLGMRATPAGVLYFHIHDPMIQSNLPLGLDEIEQEIFKKFKMKGL
LLGDQEVVRLMDTTLQEGRSNIINAGLKKDGSLRSDSAAVGQKEFDLLTKHVRRTFQEAGEQITDGRVSIEPYKMKNKTP
CTYCAFKSVCQFDESLEENEYRPLKAEKDKTILEWIKKEADGNEHS

Nucleotide


Download         Length: 3501 bp        

>NTDB_id=995561 AAVB50_RS17945 WP_345806468.1 3352142..3355642(-) (addB) [Bacillus subtilis strain FUA2233]
TTGGGAGCAGAGTTTTTAGTAGGCAGGTCCGGGAGTGGAAAAACGAAGCTGATCATCAACAGCATTCAGGATGAATTGCG
CCGGGCTCCATTCGGGAAGCCGATCATTTTTCTAGTCCCGGATCAAATGACGTTTTTAATGGAATACGAGCTTGCTAAAA
CGCCAGATATGGGCGGGATGATACGCGCTCAAGTGTTCAGTTTTTCACGATTGGCCTGGCGCGTCCTCCAGCATACGGGA
GGAATGAGCAGGCCGTTTCTGACGAGCACCGGCGTACAAATGCTCCTGCGGAAGCTCATTGAGGAGCATAAACAGGAGTT
CAAAGTCTATCAAAAAGCGAGTGACAAAAGCGGGTTTACCGCACAGGTTGAGCGGATGCTGACAGAGTTTAAGCGCTATT
GTCTGGAACCGGAGGATATCCGCCGGATGGCGGAAAGCGGAACGGCTTCTGAGTATCGCGGAGAACGAGTTTTATCTGAA
AAGCTCCATGACTTATCAATTCTGTATCAGCAAATGGAAAAAAGCCTCGCAGATCAATATCTTCACTCTGAGGATTATTT
GACATTGCTGGCAGAGCATATCCCGCTTGCGGAAGATATAAAAGGCGCCCATATCTATGTGGATGGCTTTTATCAGTTTA
CCCCGCAGGAATTCAGGGTGTTGGAGCAGCTTATGGTTCATGCGGAGCATATCACGTTTTCGCTCACAGCGGACAAGCCG
TCATATGAGCGGGAGCCGCATGAACTGGAATTGTTCAGAATGACGGGGAAAACCTATTACCGCCTGCATCAGAAGGCGAA
GGAACTGAATTTGGACATTACGTATAAAGAGCTGAGCGGGACTGAGCGGCATACAAAGACGCCGGAATTGGCGCATCTAG
AGGCACAGTATGAAGCGCGTCCGGCCATTCCATACTCAGAAAAACAAGAAGCCCTTACTGTGATGCAGGCCGCAAACAGA
CGAGCTGAGCTGGAAGGCATTGCTCGGGAAATTCACGCCCTAGTCAGAGAGAAGGGATATCGCTATAAGGATGTAGCAAT
TCTTGCGCGCCAGCCGGAAGACTACAAGGATATGGTGAAGGAAGTTTTCGCAGATTACGAGATTCCTTATTTCATTGACG
GAAAAGCATCTATGCTGAACCATCCGTTAATTGAATTTATCCGGTCAAGCCTTGATGTTCTGAAAGGGAATTGGCGTTAT
GAAGCGGTGTTTCGCTGCGTGAAAACCGAACTGCTATTCCCACTCAATGAACCGAAGGCCAAAGTGAGAGAACAGGTCGA
TCAGCTCGAAAATTACTGTATCGCCTATGGTATTAAAGGCGACCGCTGGACAAAGGGCGATCGGTTCCAATACAGGCGTT
TTGTGTCATTGGATGATGATTTTGCGCAGACTGATCAGGAAATCGAAATGGAAAACATGTTGAATGACACCCGCGATTGG
ATTGTTCCGCCGCTTTTTCAGCTTCAAAAACGCATGAAAAAAGCGAAGACGGTTCAAGAGAAGGCGGAGGCGCTCTATCG
TTATTTAGAAGAGACGGATGTGCCGCTGAAGCTGGATCAGGAAAGGCAGCGTGCTGAGGATGACGGCAGAATCATTGAAG
CGCAGCAGCATCAGCAGGCGTGGGACGCAGTCATTCAGCTGCTTGAGGAGTTTGTTGAAATGATGGGAGATGATGAGATT
TCTCTTGATTTGTTTCAGCAAATGATAGAAGCCGGCGCGGAGTCGCTTACGTTTTCTCTAATTCCGCCTGCGCTTGACCA
GGTGTTTGTCGGCAATATGGATTTGTCCAGAATGTATGGCACCTCCTGCACCTTTGTGCTCGGGGCAAACGACGGCGTTC
TGCCGGCACGCCCTGATGAAAACGGGGTCCTGTCGGATGATGACCGGGAATGGCTGAAAACGATTGGGGTTGAGCTATCC
TCAGGCGGACGGGAGCGTTTGCTTGAAGAGCACTTCCTCATCTACATGGCGTTTTCAAGTCCGTCTGACCGGCTTTACGT
ATCGTATCCGATTGCTGATGCAGAAGGAAAAACGCTTTTGCCGTCGATGATCGTTAAGCGGCTGGAAGAACTGTTTCCGC
ATCATAAGGAGCGCCTGTTAACAAATGAACCTGAACAGGTCAGCGATGAGGAACAGCTGATGTATGTTGTGAATAAAAGC
GTGGCACAGTCCTTTACCGCGAGCCAGCTCAGGTTATGGACTCGGGAATACGACATCAGCGACGTCTGGTGGAGCACGTA
CAATGTGCTGATGAGTGAGCAGGACAGGCTGCAATCGAAAAAGCTGTTCTCAAGCCTGTTTTTCCGGAATGAAGTGAAGC
AGCTTGAACGCAGCGTGTCGAGACAGCTCTATGGTGAACGCATTCAGGGCAGTGTATCGAGAATGGAAACCTTTAACGCA
TGCCCGTTTTCCCATTTTGCGTCACACGGGCTGCATTTGAAGGAACGGCAATTCTTCAAGCTTGAAGCACCGGATATCGG
CCAGCTGTTTCATTCCAGCTTAAAGCTGATTTCGGACAGGCTGCGTGAGCAAAAGCTGGATTGGCGCGATTTAACGAAGG
AGCAGTGCGAGCTGTTTTCCTATGATGCGGTAGAGCGGCTGGCACCGAAACTGCAAAAGGAAATTCTGCTCAGCTCAAAC
CGGCATTATTATGTGAAGGAAAAACTGCAAAAAATTGTGACACGTGTGTCCGGCATTTTAAGCGAGCATGCGAAAGCGAG
CGGATTCGTACCGATCGGGCTTGAACTGGGCTTTGGAGGAAAAGGGCCGCTTCCGCCGCTGACCTTTCAACTGAAAAACG
GCTGTACGATGGAACTCGTCGGGCGAATTGACCGTGTTGATAAGGCTGAAAGCTCAAAAGGCCTGCTCCTCAGGATTGTC
GATTATAAATCAAGCGACAAAGGCCTTGACTTAGCGGAAGTATATTACGGATTGGCACTGCAAATGCTGACGTACCTTGA
TTTATCGATTACACATTCAGCTGACTGGCTCGGGATGAGGGCGACGCCTGCCGGAGTGCTGTATTTCCATATTCATGACC
CGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTG
CTCCTCGGTGATCAGGAAGTGGTTCGCCTCATGGATACAACCCTTCAAGAGGGACGGTCAAATATCATAAACGCCGGCTT
GAAAAAAGACGGATCTCTTAGATCAGACTCGGCAGCAGTCGGTCAAAAGGAATTTGATCTTTTGACAAAGCATGTGCGCC
GCACCTTCCAGGAAGCGGGCGAACAAATCACCGACGGGCGCGTATCCATTGAGCCGTACAAAATGAAGAACAAGACGCCG
TGCACATACTGTGCGTTCAAATCAGTATGCCAATTTGATGAATCACTGGAAGAAAACGAGTATCGCCCATTAAAGGCTGA
AAAGGACAAGACAATACTTGAGTGGATAAAAAAGGAGGCGGATGGCAATGAACATTCCTAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)

UvrD-like helicase C-terminal domain (UvrD_C)

(306-669)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

99.743

100

0.997

Multiple sequence alignment