Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   AAVC46_RS00930 Genome accession   NZ_CP154913
Coordinates   171324..174833 (+) Length   1169 a.a.
NCBI ID   WP_334748548.1    Uniprot ID   -
Organism   Bacillus pumilus strain FUA2119     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 166324..179833
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAVC46_RS00900 (AAVC46_00900) lepB 166831..167343 (+) 513 WP_034663490.1 signal peptidase I -
  AAVC46_RS00905 (AAVC46_00905) - 167378..168523 (-) 1146 WP_334750255.1 AbrB family transcriptional regulator -
  AAVC46_RS00910 (AAVC46_00910) - 168790..169212 (+) 423 WP_024425495.1 BlaI/MecI/CopY family transcriptional regulator -
  AAVC46_RS00915 (AAVC46_00915) - 169205..170044 (+) 840 WP_340972290.1 M56 family metallopeptidase -
  AAVC46_RS00920 (AAVC46_00920) - 170102..170512 (+) 411 WP_034663477.1 DoxX family protein -
  AAVC46_RS00925 (AAVC46_00925) - 170710..171156 (-) 447 WP_034663474.1 ferritin-like domain-containing protein -
  AAVC46_RS00930 (AAVC46_00930) addB 171324..174833 (+) 3510 WP_334748548.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  AAVC46_RS00935 (AAVC46_00935) addA 174811..178515 (+) 3705 WP_340972293.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  AAVC46_RS00940 (AAVC46_00940) sbcD 178542..179717 (+) 1176 WP_340972296.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1169 a.a.        Molecular weight: 134823.76 Da        Isoelectric Point: 5.1987

>NTDB_id=995285 AAVC46_RS00930 WP_334748548.1 171324..174833(+) (addB) [Bacillus pumilus strain FUA2119]
MEIQFLAGRSGSGKTTAILEEIKEQLRLDPLGPPIIFLVPDQMTFLMEYELAKTSEAGGMIRAKVFSFTRLAWSILQQTG
GANRQFVTSTGIQMLLRKVIEEQKEKFKVFKKASDKPGFVEQIEKTMAEFKRYCMLPEDIEKISMSSMYSEYTEERRAAE
KLHDLHVLYQQMEQHLQDEYVHSEDYLTLLTQQIPSSEEIKGAHIYIDGFYQFTPQQLLVIEQLLLHAAKITAAFTVDRS
YHDRQPNELDLFRMTGKTYFQLYQLARECGADISENLLERNNRHIYTPDLAYLEHQYEQRPVQPYQENTPHLTVSKSANK
RAEIEGVARDILDLVREKGLRLRDISIVARHVDDYKDTLKEVFRDYDIPFFIDGNESMQYHPLIELIRSSLDVIKGNWRY
EAVFRCVKTEFLFPLELAKNKAREQADQLENYCIAYGVKGERWTNGSRFHYRRFQSLDEDFGQTDQEIEMEQMLNDVKEW
IVPPLYQLQKRLKKAQKVRDMVEAVYVFLEEIQVPEKLEKERLEAEEAGRLAEAMQHGQVWDAVIQLMDEFVEMLGEEEL
SFPLFQQMMDTGLASLKFALIPPSLDQVFIGSMDLSRMYQVKCTFIVGVNDGVIPARPSDESVLSEDDREWLKRAGAELA
ETGKERLLDEQFLIYQALSSPSRHLYLSYSAADAEGRSLLPSTLIKYCQELMPNHQQALYVLDPEQLDDEEQLKFVANEH
VSLSYTVSQLQQWLNQYPISGVWWSVYNYLMTSPNRDVSKNIMSSLFFTNRAKPLKPNVTKELYGDHIQGSVSRMEKFNA
CAFSHFASHGLKLKDRQFYKLEAPDIGQLFHSALKHISDTLVEQKKDWKNLTKEDCVTYSKHAIEQLAPRLQKEILLSSN
RHAYIKEKLQKILIRVSSILSEHAKVSGFSPVGLELGFGGQGPLPPFTFQLKNGCTMELVGRIDRVDKAEGSKGLLLRIV
DYKSSEKGLDLAEVYYGLALQMLTYLDLTITYSKEWLGVEATPAGILYFHIHDPLIQAPIPLAEDDIEQEIFKKFKMKGL
LLEDVEAVKLMDQTLDSGRSQVIQAGLKKDGSFRSDSAVLSEDHFHILTQHVRRTFEEAGERITNGEVAINPYKLKDQTP
CRFCSFKSICQFDESIEDNDFRVLTSEKDDVVIDRIKKEGDQYANTKTE

Nucleotide


Download         Length: 3510 bp        

>NTDB_id=995285 AAVC46_RS00930 WP_334748548.1 171324..174833(+) (addB) [Bacillus pumilus strain FUA2119]
GTGGAGATTCAGTTTTTAGCAGGCAGATCGGGGAGTGGAAAAACGACTGCAATCTTAGAGGAAATCAAAGAACAGCTTCG
GCTTGATCCGTTGGGTCCGCCAATCATTTTTTTAGTCCCGGATCAAATGACATTTTTAATGGAATATGAGCTTGCAAAAA
CGTCTGAAGCTGGCGGAATGATCAGGGCCAAAGTATTTAGTTTCACACGTCTTGCTTGGTCTATTTTACAGCAGACTGGC
GGAGCGAACCGGCAATTTGTGACAAGCACAGGAATTCAAATGCTTTTAAGAAAAGTGATTGAAGAGCAGAAAGAAAAGTT
TAAAGTATTCAAAAAAGCGAGTGATAAGCCGGGATTTGTGGAGCAAATTGAAAAGACGATGGCTGAATTCAAAAGGTATT
GTATGTTGCCGGAGGATATTGAAAAAATTTCAATGTCGAGCATGTATTCAGAGTATACAGAAGAAAGACGGGCAGCCGAA
AAATTGCATGATCTGCATGTTCTTTATCAGCAGATGGAGCAGCATTTACAAGATGAATATGTGCACTCAGAAGACTATTT
GACACTTCTCACGCAGCAAATTCCTTCATCTGAAGAAATAAAAGGAGCACACATTTATATAGATGGTTTTTATCAATTTA
CACCGCAGCAGCTTCTTGTCATTGAACAGCTCCTGCTGCATGCAGCAAAAATAACTGCTGCTTTCACAGTGGATCGCTCC
TATCATGATAGGCAGCCAAATGAACTTGATTTATTTCGTATGACAGGGAAAACGTATTTCCAGCTCTATCAGCTTGCTAG
AGAGTGCGGAGCTGACATTTCTGAAAACCTTTTGGAAAGAAATAACCGGCACATTTATACACCAGATCTTGCTTATTTAG
AACACCAATATGAGCAGAGACCAGTGCAGCCATATCAGGAAAACACCCCTCATCTCACAGTGTCTAAAAGTGCAAATAAA
CGAGCTGAAATTGAGGGTGTAGCGAGAGATATACTTGATTTAGTGAGAGAAAAAGGACTTAGATTGAGAGATATCTCAAT
TGTTGCACGGCATGTAGACGACTATAAAGATACGTTAAAAGAGGTTTTTCGAGATTACGATATTCCTTTTTTTATTGATG
GAAATGAATCGATGCAGTATCATCCGCTCATTGAATTGATTCGTTCGAGCTTGGATGTCATAAAGGGAAACTGGCGGTAT
GAAGCGGTATTTCGCTGCGTGAAAACAGAGTTCTTATTTCCGCTTGAACTCGCTAAGAACAAAGCGAGAGAGCAGGCAGA
TCAGCTCGAAAACTATTGTATTGCTTATGGTGTAAAAGGAGAACGCTGGACAAACGGTTCCCGGTTTCATTACAGGCGCT
TCCAATCATTAGATGAGGATTTTGGACAAACAGATCAAGAAATTGAAATGGAACAAATGCTAAATGACGTCAAAGAATGG
ATCGTGCCCCCGCTTTACCAGTTGCAGAAAAGGCTCAAAAAAGCGCAAAAGGTAAGAGACATGGTAGAGGCTGTCTATGT
CTTTTTAGAAGAGATACAAGTACCAGAGAAGCTTGAAAAAGAGAGGCTTGAGGCTGAAGAAGCAGGTAGATTAGCTGAAG
CAATGCAGCACGGACAAGTATGGGATGCGGTTATTCAATTAATGGATGAATTTGTTGAAATGCTAGGTGAAGAAGAGCTC
TCATTTCCTTTATTCCAACAAATGATGGATACAGGATTAGCTTCTCTAAAATTCGCTTTGATTCCGCCTTCACTCGACCA
AGTATTTATTGGAAGTATGGATTTATCGAGAATGTATCAAGTGAAGTGTACGTTTATCGTTGGTGTAAATGATGGCGTTA
TTCCCGCACGCCCCTCTGATGAGAGTGTATTGTCTGAGGATGACCGTGAATGGTTAAAACGAGCAGGAGCAGAGCTGGCA
GAGACAGGAAAGGAACGGCTGCTAGATGAGCAATTTTTAATTTACCAAGCGTTATCGAGTCCATCACGTCATTTGTATCT
ATCCTATTCGGCTGCTGATGCAGAAGGACGTTCTCTTTTGCCTTCGACACTCATCAAGTATTGCCAAGAGCTTATGCCAA
ATCATCAGCAGGCTCTTTATGTGTTAGATCCAGAACAACTGGATGACGAGGAGCAATTAAAATTTGTAGCGAATGAGCAT
GTTTCGTTATCGTATACCGTCTCACAGCTGCAGCAATGGCTCAATCAATATCCCATTAGTGGTGTATGGTGGAGTGTCTA
TAACTACTTAATGACATCTCCTAATCGAGATGTATCAAAAAACATCATGTCAAGTTTGTTTTTTACAAATCGAGCGAAGC
CATTAAAGCCTAATGTCACAAAAGAGCTTTATGGTGATCATATTCAGGGCAGTGTTTCAAGAATGGAGAAATTCAATGCG
TGTGCATTTTCTCATTTTGCTTCCCATGGGTTAAAACTAAAGGATCGACAATTTTACAAATTGGAAGCACCTGATATCGG
CCAGTTGTTTCACTCGGCTTTAAAACATATTTCAGATACGCTTGTTGAACAAAAAAAGGATTGGAAAAACCTAACGAAGG
AAGATTGTGTCACTTATTCAAAACATGCGATTGAACAGCTTGCTCCGCGTCTTCAAAAGGAAATTCTATTAAGCTCTAAT
CGGCATGCTTATATTAAAGAAAAGCTCCAGAAAATCTTGATTCGAGTCTCTTCTATATTAAGTGAACATGCCAAAGTGAG
TGGATTTTCTCCAGTAGGGCTAGAGTTAGGATTTGGGGGGCAAGGGCCATTGCCGCCGTTTACTTTTCAATTAAAGAATG
GCTGCACGATGGAGCTTGTCGGAAGAATTGACCGGGTAGATAAAGCAGAAGGTTCAAAAGGGCTGCTCTTGAGAATAGTC
GACTACAAATCCAGTGAGAAAGGTCTTGACTTAGCAGAAGTATACTATGGGCTTGCCTTGCAAATGCTCACTTACTTAGA
CCTGACCATTACGTATTCGAAAGAGTGGCTCGGGGTTGAAGCGACGCCTGCTGGCATTTTATATTTCCATATCCATGATC
CGCTCATTCAAGCACCTATTCCGCTTGCAGAGGATGACATTGAGCAGGAAATATTCAAGAAATTTAAAATGAAGGGTTTA
TTGCTTGAAGATGTAGAAGCAGTAAAGCTCATGGATCAAACGCTTGATTCAGGTAGATCACAAGTCATTCAAGCCGGCTT
GAAAAAGGATGGGTCTTTCCGTTCTGACTCTGCAGTTTTAAGTGAAGATCATTTCCATATACTCACACAACACGTTCGGC
GCACATTTGAAGAAGCAGGCGAAAGAATTACAAATGGAGAGGTCGCAATTAATCCATATAAATTGAAGGATCAAACACCT
TGCCGCTTTTGTTCATTCAAGTCTATTTGCCAATTCGATGAATCAATAGAGGATAATGATTTTAGGGTGCTCACCTCTGA
GAAAGATGATGTTGTGATAGACCGGATCAAAAAAGAAGGGGATCAGTATGCAAATACCAAAACCGAATAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

66.408

99.316

0.66

Multiple sequence alignment