Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   AAVC94_RS05280 Genome accession   NZ_CP154906
Coordinates   1064736..1068245 (+) Length   1169 a.a.
NCBI ID   WP_253622756.1    Uniprot ID   -
Organism   Bacillus pumilus strain FUA2115     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1059736..1073245
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAVC94_RS05250 (AAVC94_05250) lepB 1060247..1060759 (+) 513 WP_041815362.1 signal peptidase I -
  AAVC94_RS05255 (AAVC94_05255) - 1060794..1061939 (-) 1146 WP_251191064.1 AbrB family transcriptional regulator -
  AAVC94_RS05260 (AAVC94_05260) - 1062206..1062628 (+) 423 WP_003210872.1 BlaI/MecI/CopY family transcriptional regulator -
  AAVC94_RS05265 (AAVC94_05265) - 1062621..1063460 (+) 840 WP_050945367.1 M56 family metallopeptidase -
  AAVC94_RS05270 (AAVC94_05270) - 1063517..1063927 (+) 411 WP_003211332.1 DoxX family protein -
  AAVC94_RS05275 (AAVC94_05275) - 1064125..1064571 (-) 447 WP_003211961.1 ferritin-like domain-containing protein -
  AAVC94_RS05280 (AAVC94_05280) addB 1064736..1068245 (+) 3510 WP_253622756.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  AAVC94_RS05285 (AAVC94_05285) addA 1068223..1071921 (+) 3699 WP_253622755.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  AAVC94_RS05290 (AAVC94_05290) sbcD 1071948..1073123 (+) 1176 WP_044139868.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1169 a.a.        Molecular weight: 134796.70 Da        Isoelectric Point: 5.3071

>NTDB_id=995049 AAVC94_RS05280 WP_253622756.1 1064736..1068245(+) (addB) [Bacillus pumilus strain FUA2115]
MEIQFLAGRSGSGKTTAILEEIKEQLRLDPLGPPIIFLVPDQMTFLMEYELAKTSEAGGMIRAKVFSFTRLAWSVLQQTG
GANRQFVTSTGIQMLLRKVIEEQKDKFKVFKKASDKPGFVEQIEKTMAEFKRYCMLPEDIEKISASSMHSEYTEERRAAE
KLHDLHVLYQQMEQHLQDEYVHSEDYLTLLSQQIPSAEEIKGAHIYIDGFYQFTPQQLLVIEQLLLYAAKITAAFTVDRS
YHDRQPNELDLFRMTGKTYFQLYKLAKECGADISENIFERNHRHLYTPDLAYLEHQYEQRPVQPYQENTPHLTVSKSANK
RAEIEGVARDILDLVREKGLRLRDISIVARHVDDYKDTLKEVFRDYDIPFFIDGNESMQYHPLIELIRSSLDVIKGNWRY
EAVFRCVKTEFLFPLEITKNKAREQADQLENYCIAYGVKGERWTNGSQFHYRRFQSLDEDFRQTDQEIEMEQMLNDVKEW
IAPPLYQLQKRLKKAQKVRDMVEAVYVFLEEIQVPDKLEKARLEAEEAGRLAEAMQHGQVWDAVIQLMDEFVDMLGDEEL
SFPLFQQMIDTGLASLKFALIPPSLDQVFIGSMDLSRMYQVKCTFIIGVNDGVIPARPSDESVLSEDDREWLKRAGAELA
ETGKERLLDEQFLIYQALSSPSRHLYLSYAASDAEGRSLLPSTLIKYCQELMPNHQQALYVLDPEQLEDDEQLKFVANEH
VSLSYTISQLQQWLNQYPISGVWWSIYNYLMTSPHRDVSKNIMSSLFFTNQAKPLKPNVTKELYGDHIQGSVSRMEKFNA
CAFSHFASHGLKLKDRQFYKLEAPDIGQLFHSALKHISDTLVEQKKDWKNLTKEDCVTYSRHAIEQLAPRLQKEILLSSN
RHAYIKEKLQKILIRVSSILSEHAKLSGFSPVGLELGFGGQGPLPPFTFQLKNGCTMELVGRIDRVDKAEGSKGLLLRIV
DYKSSEKGLDLAEVYYGLALQMLTYLDLTITYSKEWLGVEATPAGILYFHIHDPLIQAPIPLAEDEIEQEIFKKFKMKGL
LLEDVEAVKLMDQTLDSGRSQVIQAGLKKDGSFRSDSAVLSEDHFHILTQHVRRTFEEAGERITNGEVAINPYKLKDQTP
CRFCSFKSICQFDESIEDNDFRVLTSEKDDVVIERIKKEGDQYANTKTE

Nucleotide


Download         Length: 3510 bp        

>NTDB_id=995049 AAVC94_RS05280 WP_253622756.1 1064736..1068245(+) (addB) [Bacillus pumilus strain FUA2115]
GTGGAGATTCAGTTTTTAGCAGGCAGATCGGGGAGTGGAAAAACGACTGCAATCTTAGAGGAAATCAAAGAACAGCTTCG
GCTTGATCCGTTGGGTCCGCCAATCATTTTTTTAGTCCCGGATCAAATGACATTTTTAATGGAATATGAGCTTGCGAAAA
CGTCTGAAGCTGGTGGAATGATCAGAGCTAAAGTATTTAGTTTCACTCGTCTTGCTTGGTCTGTTTTACAGCAGACTGGT
GGAGCGAATCGGCAATTTGTGACAAGCACAGGAATTCAAATGCTTTTAAGAAAAGTGATTGAAGAGCAGAAAGACAAGTT
TAAAGTATTCAAAAAAGCGAGTGATAAGCCGGGGTTTGTGGAGCAAATCGAAAAAACGATGGCTGAATTCAAAAGATACT
GTATGCTGCCAGAGGATATTGAGAAGATTTCAGCGTCTAGCATGCATTCAGAGTATACAGAAGAAAGACGGGCAGCCGAA
AAATTGCATGATCTCCATGTTCTTTATCAGCAGATGGAGCAGCATTTACAAGATGAATATGTGCACTCAGAAGACTATTT
GACTCTTCTTTCCCAGCAAATTCCTTCAGCTGAAGAAATAAAAGGAGCACACATCTATATAGATGGTTTTTATCAATTTA
CACCGCAGCAGCTTCTTGTCATTGAACAGCTCTTGCTGTATGCAGCAAAAATAACTGCGGCTTTCACAGTAGATCGATCC
TATCATGATAGGCAGCCAAATGAACTTGATTTATTTCGTATGACAGGTAAAACGTATTTCCAGCTCTATAAGCTTGCTAA
AGAGTGCGGAGCTGACATTTCTGAAAATATTTTTGAAAGAAATCATCGGCACCTTTATACACCAGATCTTGCTTATTTAG
AACACCAATATGAGCAGCGGCCCGTTCAGCCATATCAGGAAAACACCCCTCATCTCACAGTGTCTAAAAGTGCAAATAAA
CGAGCTGAAATTGAGGGAGTAGCGAGAGATATCCTTGATTTAGTGAGAGAAAAAGGACTCAGACTGCGAGATATCTCGAT
TGTAGCGCGGCATGTAGATGACTATAAAGATACGTTAAAAGAGGTTTTTCGAGATTACGATATTCCTTTTTTTATTGATG
GAAATGAATCGATGCAGTATCATCCGCTCATTGAATTAATTCGTTCGAGCTTGGATGTCATAAAAGGAAATTGGCGGTAT
GAAGCGGTATTTCGCTGCGTGAAAACAGAGTTCTTATTTCCGCTTGAAATCACTAAGAACAAAGCGAGAGAGCAGGCAGA
TCAGCTTGAAAACTATTGCATTGCTTATGGTGTAAAAGGAGAGCGCTGGACAAACGGTTCCCAGTTTCATTACAGGCGTT
TCCAATCATTAGATGAGGATTTCAGGCAAACAGATCAAGAAATTGAAATGGAACAAATGCTAAATGACGTCAAAGAATGG
ATCGCGCCCCCGCTTTACCAGTTGCAGAAAAGGCTCAAAAAAGCGCAAAAGGTAAGGGACATGGTAGAGGCTGTCTATGT
CTTTTTAGAAGAGATACAAGTACCAGATAAGCTTGAAAAAGCGAGGCTTGAGGCTGAAGAAGCAGGTCGATTAGCTGAAG
CAATGCAGCACGGGCAAGTATGGGATGCGGTGATTCAATTAATGGATGAATTTGTTGACATGCTAGGTGACGAAGAGCTC
TCATTTCCCTTATTCCAACAGATGATAGATACAGGATTAGCTTCTCTAAAATTCGCTTTGATTCCGCCTTCACTCGACCA
AGTATTTATTGGAAGTATGGATTTATCCAGAATGTATCAAGTGAAGTGTACGTTTATCATTGGTGTAAATGATGGCGTTA
TTCCCGCACGTCCCTCTGATGAGAGTGTATTGTCTGAGGATGACCGAGAATGGTTAAAACGAGCAGGAGCAGAGCTAGCA
GAGACAGGAAAAGAACGGCTGCTAGATGAGCAATTTTTAATTTACCAAGCGTTATCAAGTCCATCCCGTCATTTGTATCT
ATCCTATGCGGCTTCTGATGCAGAAGGACGTTCTCTATTGCCTTCGACACTCATCAAGTATTGCCAAGAGCTTATGCCAA
ATCATCAACAGGCTCTTTATGTGTTAGATCCAGAACAACTGGAAGACGATGAGCAATTAAAATTTGTAGCGAATGAGCAT
GTTTCGTTGTCGTATACCATCTCACAGCTGCAGCAATGGCTCAATCAATATCCCATCAGCGGTGTATGGTGGAGTATCTA
TAACTACTTAATGACATCTCCTCATCGGGATGTATCAAAAAACATCATGTCAAGCTTGTTCTTTACAAATCAAGCGAAGC
CATTAAAGCCTAATGTCACAAAAGAGCTTTACGGTGACCATATTCAGGGCAGTGTTTCAAGAATGGAGAAGTTCAATGCG
TGTGCATTCTCTCACTTTGCTTCCCACGGTTTAAAACTAAAGGATCGACAATTTTACAAATTGGAAGCACCCGATATCGG
CCAATTGTTTCACTCGGCTTTGAAGCATATTTCAGATACGCTAGTTGAACAAAAAAAGGATTGGAAAAACCTAACGAAGG
AAGATTGTGTCACCTATTCAAGACATGCAATCGAACAGCTTGCTCCGCGTCTTCAAAAGGAAATTCTATTAAGCTCTAAT
CGGCATGCTTATATTAAAGAAAAGCTTCAGAAAATCTTGATTCGAGTCTCTTCTATATTAAGTGAACATGCCAAATTGAG
TGGATTTTCTCCGGTAGGGCTTGAGTTAGGTTTTGGAGGTCAAGGGCCTTTGCCGCCGTTTACTTTTCAATTAAAGAATG
GCTGCACGATGGAGCTTGTCGGAAGGATTGACAGGGTAGATAAGGCAGAAGGTTCAAAAGGGCTGCTTTTAAGAATCGTC
GATTACAAATCCAGTGAGAAAGGTCTTGACCTAGCAGAAGTATACTATGGTCTTGCCTTGCAAATGCTCACATACTTAGA
CCTAACCATTACGTATTCGAAAGAGTGGCTCGGCGTAGAAGCAACGCCTGCTGGCATTTTATATTTCCATATTCATGATC
CGCTCATTCAAGCACCGATTCCACTTGCAGAGGATGAAATTGAGCAGGAAATCTTCAAGAAATTTAAAATGAAGGGTTTA
TTGCTTGAAGATGTGGAAGCAGTAAAGCTAATGGATCAAACGCTTGATTCAGGTAGATCGCAAGTCATTCAAGCCGGTTT
GAAAAAGGATGGGTCTTTCCGATCTGATTCGGCAGTTTTAAGTGAGGATCATTTCCATATACTCACACAACACGTTCGGC
GCACATTTGAAGAAGCAGGCGAAAGAATTACAAATGGAGAGGTCGCAATTAATCCATACAAATTAAAGGATCAAACACCT
TGCCGCTTTTGTTCATTCAAGTCTATTTGCCAATTCGATGAATCAATAGAAGATAATGATTTTAGGGTGCTCACCTCTGA
AAAGGATGATGTTGTGATAGAACGGATCAAAAAAGAAGGGGATCAGTATGCAAATACCAAAACCGAATAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

66.236

99.316

0.658

Multiple sequence alignment