Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   EJN50_RS09265 Genome accession   NZ_LR130527
Coordinates   1920869..1921294 (+) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa isolate paerg002     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1915869..1926294
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EJN50_RS09250 (BANRA_01805) pilX 1916433..1917020 (+) 588 WP_003112826.1 type 4a pilus minor pilin PilX -
  EJN50_RS09255 (BANRA_01806) pilY1 1917032..1920523 (+) 3492 WP_003123397.1 type 4a pilus biogenesis protein PilY1 -
  EJN50_RS09260 (BANRA_01807) pilY2 1920525..1920872 (+) 348 WP_003102609.1 type 4a fimbrial biogenesis protein PilY2 -
  EJN50_RS09265 (BANRA_01808) comF 1920869..1921294 (+) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  EJN50_RS09270 (BANRA_01809) ispH 1921341..1922285 (-) 945 WP_003094724.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  EJN50_RS09275 (BANRA_01810) fkpB 1922371..1922811 (-) 441 WP_003102613.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  EJN50_RS09280 (BANRA_01811) lspA 1922804..1923313 (-) 510 WP_003102615.1 signal peptidase II -
  EJN50_RS09285 (BANRA_01812) ileS 1923306..1926137 (-) 2832 WP_014603309.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=995035 EJN50_RS09265 WP_003094721.1 1920869..1921294(+) (comF) [Pseudomonas aeruginosa isolate paerg002]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=995035 EJN50_RS09265 WP_003094721.1 1920869..1921294(+) (comF) [Pseudomonas aeruginosa isolate paerg002]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGTTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCGCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAACCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383

Multiple sequence alignment