Detailed information NTG0994769

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: EW029_RS05735
• Genome accession: NZ_LR130513
• Coordinates: 1159560..1161968 (+)
• Length: 802 a.a.
• NCBI ID: WP_000560832.1
• Uniprot ID: -
• Organism: Staphylococcus aureus strain BPH2900 isolate BPH2900
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 1154560..1166968

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EW029_RS05710	-	1154668..1155069 (+)	402	WP_000354184.1	VOC family protein	-
EW029_RS05715	-	1155332..1157029 (-)	1698	WP_000312772.1	Rqc2 family fibronectin-binding protein	-
EW029_RS05720	gmk	1157304..1157927 (+)	624	WP_000368225.1	guanylate kinase	-
EW029_RS05725	rpoZ	1157927..1158145 (+)	219	WP_000933956.1	DNA-directed RNA polymerase subunit omega	-
EW029_RS05730	coaBC	1158361..1159560 (+)	1200	WP_000722165.1	bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC	-
EW029_RS05735	priA	1159560..1161968 (+)	2409	WP_000560832.1	primosomal protein N'	Machinery gene
EW029_RS05740	-	1162474..1163427 (+)	954	WP_000751697.1	hypothetical protein	-
EW029_RS05745	-	1163590..1163793 (-)	204	WP_001198020.1	TM2 domain-containing protein	-
EW029_RS05750	-	1164092..1164580 (+)	489	WP_000985287.1	peptide deformylase	-
EW029_RS05755	fmt	1164573..1165508 (+)	936	WP_000161285.1	methionyl-tRNA formyltransferase	-
EW029_RS05760	rsmB	1165505..1166812 (+)	1308	WP_000572233.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-

Sequence

Protein

• Length: 802 a.a.
• Molecular weight: 92522.40 Da
• Isoelectric Point: 6.6581

>NTDB_id=994769 EW029_RS05735 WP_000560832.1 1159560..1161968(+) (priA) [Staphylococcus aureus strain BPH2900 isolate BPH2900]
MIAKVIVDVASKSVDFKFDYIIPEQLESVIQPGVRVIVPFGPRTIQGYVMEVTAEPDAQLDVSKLKKIIEVKDIQPELTS
ELIALSEWMGSTHVIKRISMLEVMLPSAIKAKYKKAFKMKDDIELPSALLQKFDKHGYYYYKDAQKNNDIQLLMKLLKDD
IVEEKTILTQNITKKTKRAVRVIEGYHPDEVLAKLEKVIKQYDLYAYLSEEQHKTIFLTDIEDMGFSKSSLDGLIKKGYV
EKYDAVVDRDPFKDRVFEQESKQQLTEDQYKAYEAIKAKIVSQEQETFLLHGVTGSGKTEVYLQTIEDVLSQGKQAMMLV
PEIALTPQMVLRFKRRFGDDVAVLHSGLSNGERYDEWQKIRDGRARVSVGARSSVFAPFKNLGLIIIDEEHESTYKQEDY
PRYHAREIAQWRSEYHHCPVILGSATPCLESYARAEKGVYHLLSLPNRVNQQALPEIDIVDMREELSEGNRSMFSKDLRE
AIQLRLDRQEQVVLFLNRRGYASFMLCRDCGYVPQCPNCDISLTYHKTTDLLKCHYCGYQETPPNQCPNCESEHIRQVGT
GTQKVEELLQQEFEDARIIRMDVDTTSKKGAHEKLLTEFEKGNGDILLGTQMIAKGLDYPNITLVGVLNADTMLNLPDFR
ASERTYQLLTQVAGRAGRHEKAGQVIIQTYNPDHYSILDVQKNDYLTFYRQEMEYRKLGKYPPYYYLINFTISHKEMKKV
MEASQHVHKILLQHLTEKALVLGPSPAALARINNEFRFQILVKYKSEPGLLQAIQFLDDYYHEKFIKEKLALKIDIDPQM
MM

Nucleotide

• Length: 2409 bp

>NTDB_id=994769 EW029_RS05735 WP_000560832.1 1159560..1161968(+) (priA) [Staphylococcus aureus strain BPH2900 isolate BPH2900]
ATGATAGCGAAAGTCATAGTCGATGTCGCGTCGAAGAGCGTTGACTTTAAATTTGATTATATAATTCCTGAACAACTCGA
ATCTGTCATCCAACCTGGTGTGCGTGTGATTGTACCTTTTGGACCAAGAACGATTCAAGGTTATGTAATGGAAGTAACAG
CAGAACCTGATGCACAACTTGACGTTTCGAAGTTAAAAAAAATCATAGAAGTGAAAGATATACAACCTGAATTAACATCA
GAATTAATAGCTTTAAGTGAGTGGATGGGTTCAACTCATGTCATTAAACGTATTTCTATGCTAGAAGTGATGCTTCCGAG
TGCTATTAAAGCGAAGTATAAAAAAGCATTTAAGATGAAAGATGACATAGAGCTACCTTCAGCTTTATTACAAAAATTTG
ATAAGCATGGTTACTATTATTATAAAGATGCGCAAAAAAATAATGATATTCAATTGCTTATGAAGTTGTTAAAAGATGAT
ATCGTTGAAGAAAAAACGATTCTCACACAAAATATAACTAAAAAAACCAAGCGTGCTGTTCGTGTCATTGAAGGGTATCA
TCCTGATGAAGTATTAGCTAAGTTGGAGAAAGTTATTAAACAATACGATTTGTATGCTTACTTGTCTGAAGAACAACATA
AAACAATATTTTTAACTGATATTGAGGATATGGGCTTTTCAAAATCCAGTTTAGATGGACTTATCAAAAAAGGTTATGTT
GAAAAATATGACGCGGTTGTTGATAGAGACCCATTTAAAGATCGTGTTTTCGAACAAGAATCAAAACAGCAATTAACAGA
AGATCAATATAAAGCATATGAAGCGATTAAAGCTAAAATTGTAAGCCAAGAGCAAGAAACATTTTTACTTCATGGTGTGA
CGGGATCAGGTAAAACAGAAGTATATTTACAAACGATAGAAGATGTTTTAAGCCAAGGAAAACAGGCGATGATGTTAGTT
CCTGAAATCGCTCTAACACCGCAAATGGTTTTACGCTTCAAACGTCGATTTGGTGATGACGTTGCTGTATTACATTCTGG
CTTATCTAATGGGGAACGTTATGATGAGTGGCAAAAAATTAGGGATGGTCGTGCGAGAGTAAGTGTTGGTGCAAGGTCAA
GTGTGTTCGCACCTTTCAAAAATTTAGGGTTAATCATCATTGATGAAGAACATGAATCTACATATAAACAAGAAGATTAT
CCGAGATATCACGCTAGAGAAATTGCCCAATGGCGAAGTGAATATCATCACTGTCCAGTCATTTTAGGAAGTGCAACACC
ATGTCTTGAAAGTTATGCACGAGCTGAAAAAGGCGTTTATCATTTGCTATCATTACCAAACAGAGTGAACCAACAAGCTT
TACCTGAAATTGATATAGTAGACATGCGTGAAGAATTGAGTGAAGGTAATCGGTCAATGTTTTCAAAAGATTTACGTGAA
GCCATACAATTAAGATTAGATCGACAGGAACAAGTTGTTTTATTTTTAAATCGACGTGGTTATGCATCGTTTATGTTATG
TCGGGATTGTGGATATGTACCGCAATGTCCAAACTGTGATATTTCATTAACGTATCATAAAACGACAGACTTATTAAAAT
GTCACTATTGTGGTTACCAAGAGACGCCACCGAATCAATGTCCAAATTGTGAGAGTGAACACATTCGACAAGTAGGTACT
GGTACTCAGAAAGTTGAAGAACTATTGCAACAAGAATTTGAAGATGCGCGCATAATTAGGATGGATGTAGATACAACCTC
AAAGAAAGGTGCACATGAAAAGTTATTGACTGAATTCGAAAAAGGTAACGGTGACATTTTACTAGGTACTCAGATGATTG
CGAAAGGATTAGATTATCCAAATATTACTTTAGTTGGTGTGCTGAATGCAGATACAATGTTAAATTTACCTGATTTTCGG
GCGAGCGAACGTACTTATCAACTATTAACGCAAGTGGCTGGTAGAGCTGGTCGTCATGAAAAGGCAGGTCAAGTCATCAT
TCAAACGTATAATCCAGATCATTATTCAATATTGGATGTTCAAAAAAATGATTATTTAACATTTTATCGTCAGGAAATGG
AATATCGTAAATTAGGAAAGTATCCACCGTATTATTATTTGATTAATTTCACAATCTCACATAAAGAAATGAAGAAGGTT
ATGGAAGCATCGCAGCATGTTCATAAAATTTTATTACAGCATTTAACAGAAAAAGCGCTTGTGCTAGGTCCATCTCCGGC
AGCACTTGCGAGAATCAACAATGAATTTAGATTCCAAATTTTAGTGAAATATAAAAGTGAACCTGGATTATTACAAGCCA
TTCAGTTTTTAGATGACTATTACCATGAAAAATTTATAAAAGAAAAATTAGCATTGAAGATTGATATTGATCCGCAGATG
ATGATGTAA

Domains

Predicted by InterProScan.

• 3DNA-binding domain (3BD) (PriA_3primeBD) (4-106)

• Type III restriction enzyme, res subunit (ResIII) (264-427)

• Helicase conserved C-terminal domain (Helicase_C) (564-659)

• Primosomal protein N C-terminal domain (PriA_C) (703-798)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (516-541)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Bacillus subtilis subsp. subtilis str. 168	50.556	100	0.51