Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   AAYT23_RS10130 Genome accession   NZ_CP154877
Coordinates   1967213..1968511 (-) Length   432 a.a.
NCBI ID   WP_000867615.1    Uniprot ID   A0AAJ5P055
Organism   Streptococcus pneumoniae strain 20234295     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1962213..1973511
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAYT23_RS10110 (AAYT23_10090) rplI 1963368..1963820 (-) 453 WP_000864220.1 50S ribosomal protein L9 -
  AAYT23_RS10115 (AAYT23_10095) - 1963817..1965790 (-) 1974 WP_000715106.1 DHH family phosphoesterase -
  AAYT23_RS10120 (AAYT23_10100) hpf 1965926..1966474 (-) 549 WP_000599104.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  AAYT23_RS10125 (AAYT23_10105) comFC/cflB 1966554..1967216 (-) 663 WP_000649971.1 ComF family protein Machinery gene
  AAYT23_RS10130 (AAYT23_10110) comFA/cflA 1967213..1968511 (-) 1299 WP_000867615.1 DEAD/DEAH box helicase Machinery gene
  AAYT23_RS10135 (AAYT23_10115) - 1968567..1969202 (+) 636 WP_000395739.1 YigZ family protein -
  AAYT23_RS10140 (AAYT23_10120) cysK 1969487..1970407 (+) 921 WP_000029895.1 cysteine synthase A -
  AAYT23_RS10145 (AAYT23_10125) - 1970482..1971020 (-) 539 Protein_1957 IS66 family transposase -
  AAYT23_RS10150 (AAYT23_10130) - 1970993..1971301 (+) 309 Protein_1958 transposase -
  AAYT23_RS10155 (AAYT23_10135) tnpB 1971456..1971779 (-) 324 WP_000973408.1 IS66 family insertion sequence element accessory protein TnpB -
  AAYT23_RS10160 (AAYT23_10140) tsf 1972025..1973065 (-) 1041 WP_000808063.1 translation elongation factor Ts -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 49576.58 Da        Isoelectric Point: 8.5992

>NTDB_id=994494 AAYT23_RS10130 WP_000867615.1 1967213..1968511(-) (comFA/cflA) [Streptococcus pneumoniae strain 20234295]
MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCNSTILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQ
EDFPKQDVLKWRGQLTPFQEKVSEGLLQVVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLDLYKR
LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPMLYHAVKNSVKENGLRIFLTATST
NELDKKVRLGELKRLNLPRRFHGNPLIIPKPIWLSDFNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLAEI
LQEQFPNEKIGFVSSVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGGRVGRSMDR
PTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=994494 AAYT23_RS10130 WP_000867615.1 1967213..1968511(-) (comFA/cflA) [Streptococcus pneumoniae strain 20234295]
ATGAAAGTAAATTTAGATTATCTCGGTCGTTTATTTACTGAGAATGAATTAACAGAAGAAGAACGTCAGTTGGCGGAGAA
ACTTCCAGCAATGAGAAAGGAGAAGGGGAAACTTTTCTGTCAACGCTGTAATAGTACTATTCTAGAAGAATGGTATTTGC
CCATCGGTGCTTACTATTGTCGAGAGTGCTTGCTGATGAAGCGAGTCAGAAGTGATCAAACTTTATACTATTTTCCGCAG
GAGGATTTTCCAAAGCAAGATGTTCTCAAATGGCGCGGCCAATTAACTCCTTTTCAAGAGAAGGTGTCAGAGGGATTGCT
TCAAGTAGTAGACAAGCAAAAGCCAACCTTAGTTCATGCGGTAACAGGAGCTGGAAAGACAGAAATGATTTATCAAGTAG
TGGCTAAAGTGATCAATGCGGGTGGTGCAGTGTGTTTGGCTAGTCCTCGCATAGATGTTTGTTTGGATCTGTACAAGCGC
CTGCAACAGGATTTTTCTTGCGGGATAGCTTTGCTACATGGAGAATCGGAACCTTATTTTCGAACACCACTAGTTGTTGC
AACAACCCATCAGTTATTGAAGTTTTATCAAGCTTTTGATTTGCTGATAGTGGATGAAGTAGATGCTTTTCCTTATGTTG
ATAATCCCATGCTTTACCACGCTGTCAAGAATAGTGTAAAGGAGAATGGATTGAGAATCTTTTTAACAGCGACTTCGACC
AATGAGTTAGATAAAAAGGTCCGTTTAGGAGAACTAAAAAGACTGAATTTACCGAGACGGTTTCATGGAAATCCGTTGAT
TATTCCAAAACCAATTTGGTTATCGGATTTTAATCGCTACTTAGACAAGAATCGTTTGTCACCAAAGTTAAAGTCCTATA
TTGAGAAGCAGAGAAAGACAGCTTATCCGTTACTCATTTTTGCTTCAGAAATTAAGAAAGGGGAGCAGTTAGCAGAAATC
TTACAGGAGCAATTTCCAAATGAGAAAATTGGCTTTGTATCTTCTGTAACAGAGGATCGATTAGAGCAAGTACAAGCTTT
TCGAGATGGAGAACTGACAATACTTATCAGTACGACAATCTTGGAGCGTGGAGTTACCTTCCCTTGTGTGGATGTTTTCG
TAGTAGAGGCCAATCATCGTTTGTTTACCAAGTCTAGTTTGATTCAGATTGGTGGACGAGTTGGACGAAGCATGGATAGA
CCGACAGGAGATTTGCTTTTCTTCCATGATGGGTTAAATGCTTCAATCAAGAAGGCGATTAAGGAAATTCAGATGATGAA
TAAGGAGGCTGGTCTATGA

Domains

Predicted by InterproScan.

DEAD/DEAH box helicase (DEAD)

(96-244)

Helicase conserved C-terminal domain (Helicase_C)

(293-396)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae TIGR4

99.769

100

0.998
  comFA/cflA Streptococcus pneumoniae Rx1

99.537

100

0.995
  comFA/cflA Streptococcus pneumoniae D39

99.537

100

0.995
  comFA/cflA Streptococcus pneumoniae R6

99.537

100

0.995
  comFA/cflA Streptococcus mitis NCTC 12261

93.056

100

0.931
  comFA/cflA Streptococcus mitis SK321

91.898

100

0.919
  comFA Lactococcus lactis subsp. cremoris KW2

52.206

94.444

0.493
  comFA Latilactobacillus sakei subsp. sakei 23K

38.979

99.769

0.389

Multiple sequence alignment