Detailed information NTG0993113

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: AAID36_RS15440
• Genome accession: NZ_CP154372
• Coordinates: 3162842..3163960 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A009PAD6
• Organism: Acinetobacter baumannii strain NCSR_106
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3157842..3168960

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AAID36_RS15430 (AAID36_15435)	-	3160958..3161650 (-)	693	WP_001108506.1	YggS family pyridoxal phosphate-dependent enzyme	-
AAID36_RS15435 (AAID36_15440)	pilT	3161777..3162814 (+)	1038	WP_000355491.1	type IV pilus twitching motility protein PilT	Machinery gene
AAID36_RS15440 (AAID36_15445)	pilU	3162842..3163960 (+)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
AAID36_RS15445 (AAID36_15450)	fur	3164030..3164467 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
AAID36_RS15450 (AAID36_15455)	bamE	3164579..3164977 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
AAID36_RS15455 (AAID36_15460)	-	3165032..3165355 (-)	324	WP_000442585.1	RnfH family protein	-
AAID36_RS15460 (AAID36_15465)	-	3165355..3166422 (-)	1068	WP_000092403.1	hypothetical protein	-
AAID36_RS15465 (AAID36_15470)	-	3166575..3167030 (-)	456	WP_000782976.1	bacteriohemerythrin	-
AAID36_RS15470 (AAID36_15475)	-	3167283..3168131 (-)	849	WP_000336554.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=993113 AAID36_RS15440 WP_000347036.1 3162842..3163960(+) (pilU) [Acinetobacter baumannii strain NCSR_106]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=993113 AAID36_RS15440 WP_000347036.1 3162842..3163960(+) (pilU) [Acinetobacter baumannii strain NCSR_106]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGCCTTTCTGGGGAAGTAATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGTGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGTGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCACAGCCAGCTTTATATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAGGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A009PAD6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392