Detailed information NTG0990660

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: V4T04_RS05345
• Genome accession: NZ_JAZICS010000003
• Coordinates: 289134..291473 (-)
• Length: 779 a.a.
• NCBI ID: WP_058204016.1
• Uniprot ID: -
• Organism: Lactococcus lactis subsp. lactis strain DGCC12653
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 284134..296473

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
V4T04_RS05305	-	285050..285556 (-)	507	WP_012898444.1	Asp23/Gls24 family envelope stress response protein	-
V4T04_RS05310	-	285577..285765 (-)	189	WP_012898445.1	DUF2273 domain-containing protein	-
V4T04_RS05315	amaP	285776..286336 (-)	561	WP_010906186.1	alkaline shock response membrane anchor protein AmaP	-
V4T04_RS05320	-	286364..286606 (-)	243	WP_003131476.1	GlsB/YeaQ/YmgE family stress response membrane protein	-
V4T04_RS05325	fmt	286781..287740 (-)	960	WP_349651780.1	methionyl-tRNA formyltransferase	-
V4T04_RS05330	-	287737..288201 (-)	465	WP_003131474.1	VOC family protein	-
V4T04_RS05335	-	288167..288688 (-)	522	WP_103055110.1	NUDIX hydrolase	-
V4T04_RS05340	-	288701..289075 (-)	375	WP_349651781.1	nuclear transport factor 2 family protein	-
V4T04_RS05345	priA	289134..291473 (-)	2340	WP_058204016.1	primosomal protein N'	Machinery gene
V4T04_RS05350	rpoZ	291612..291965 (-)	354	WP_010906189.1	DNA-directed RNA polymerase subunit omega	-
V4T04_RS05355	gmk	292145..292762 (-)	618	WP_003131468.1	guanylate kinase	-
V4T04_RS05360	rny	292910..294505 (-)	1596	WP_003131467.1	ribonuclease Y	-
V4T04_RS05365	-	294736..295449 (-)	714	WP_003131466.1	glucosamine-6-phosphate deaminase	-

Sequence

Protein

• Length: 779 a.a.
• Molecular weight: 88929.26 Da
• Isoelectric Point: 6.5580

>NTDB_id=990660 V4T04_RS05345 WP_058204016.1 289134..291473(-) (priA) [Lactococcus lactis subsp. lactis strain DGCC12653]
MKIAKVIVDVPLMQTDKPFSYSLSAEFENLLEVGMRVHVPFGSGNRLVQGIVIGFEDEELEPSVNLKGISELLDVEPVLT
DEQLWLADEMRHSVFSYKISCLKAMLPNLLNSNYDKLLKSTDGTSVKWSQLTEAEKISSAKAVRLGQLSVEYLAKSKENR
KTEKFLSIPDIEKLAEFEIEKRAKKRLELKYFLLRLQEEKIAVKSLNFSSQIIKFFVEHDLVKIEEVEISRTKEKFEDVE
NDSAKILNSEQNAAYEKIIASSDKPFLLEGITGSGKTEVYLQTIDHFLKLGKTAIMLVPEISLTPQITNRFIARFGDKVA
IMHSGLSDGEKYDEWRKIKSGKAQVVVGARSAIFAPVENLGIIIIDEEHETSYKQDSSPRYHARDIALLRAKWHGARLVL
GSATPSLETRARAMRGVYELLLLTKRANEAAILPEIKIIDMRSEISDQSANFSQSLLTKIKEKIDKDEQVILMLNRRGYS
SFVMCRECGFVVDCPNCDISLTLHMDTRTLNCHYCGHVEGIPQTCPNCHSRKIRYYGSGTQKVEEELREIMPEARVLRMD
VDTTRTKNAHEKILEKFGNHEADILLGTQMIAKGLDFPNVTLVGVINADTGLNLPDFRASERSFELLMQVAGRAGRADKK
GEVLIQTFNPHHYAIEMTKAQDYEKFYEMEMRFRRELSYPPYYFTVQVLVSHQDEEVAIQKSYEIAQLLKQNLTEKAKIL
GPTARPIARTHNLYHYQILVKYRFEEELESALNQVLELTQLRDNKNLRVLIDSEPQNFV

Nucleotide

• Length: 2340 bp

>NTDB_id=990660 V4T04_RS05345 WP_058204016.1 289134..291473(-) (priA) [Lactococcus lactis subsp. lactis strain DGCC12653]
ATGAAAATTGCTAAAGTCATCGTTGATGTGCCTCTGATGCAGACGGATAAACCCTTTTCTTACAGCCTTTCAGCTGAATT
TGAAAATTTGCTTGAAGTGGGCATGCGGGTTCATGTTCCTTTTGGAAGTGGAAACCGGCTTGTTCAAGGAATTGTCATTG
GATTTGAAGATGAAGAACTTGAGCCGTCAGTAAATTTGAAAGGAATTTCTGAACTACTCGACGTTGAGCCAGTCCTAACG
GATGAACAGCTCTGGTTGGCAGATGAAATGCGTCATTCAGTATTTTCTTATAAAATTTCATGTCTAAAAGCCATGTTACC
TAATCTTTTGAATTCTAATTATGACAAGCTCCTAAAATCGACTGACGGAACTTCTGTCAAATGGTCACAACTGACAGAAG
CAGAAAAAATCTCGTCAGCAAAGGCTGTACGTTTGGGTCAGCTGTCAGTAGAATATTTAGCAAAATCTAAAGAAAATAGA
AAAACAGAAAAATTCTTATCTATTCCAGATATCGAAAAACTAGCTGAATTTGAAATTGAAAAGAGAGCTAAAAAACGCTT
AGAGTTGAAATATTTTCTATTAAGACTTCAAGAAGAAAAAATTGCAGTCAAATCATTAAATTTTTCTTCACAAATCATTA
AATTTTTCGTTGAGCATGACCTAGTCAAAATTGAAGAGGTTGAAATTTCGAGGACGAAAGAAAAATTTGAAGATGTGGAA
AATGACAGTGCTAAAATTCTCAACTCCGAACAAAATGCAGCTTACGAAAAAATAATTGCATCTTCTGACAAACCATTTTT
GTTGGAAGGAATTACTGGTTCAGGTAAAACAGAAGTTTACTTACAGACCATTGACCATTTTCTCAAACTCGGTAAAACAG
CAATTATGCTTGTGCCAGAAATTTCTTTGACGCCACAGATTACTAATCGATTTATTGCTCGTTTTGGAGATAAAGTGGCG
ATTATGCATTCTGGTTTATCTGATGGTGAAAAATATGATGAATGGCGAAAAATTAAATCTGGCAAAGCTCAGGTAGTTGT
CGGTGCACGTTCAGCTATTTTTGCACCTGTTGAAAATTTGGGAATTATCATCATTGATGAAGAACATGAAACTTCATATA
AGCAAGATTCAAGTCCCCGTTATCATGCAAGAGATATTGCGCTTTTGCGTGCAAAATGGCATGGTGCAAGACTTGTTTTA
GGTTCTGCAACGCCAAGTTTAGAAACGCGAGCACGTGCCATGCGCGGAGTTTATGAACTTTTATTACTGACAAAAAGGGC
AAATGAAGCAGCAATCCTGCCAGAAATAAAAATCATTGATATGCGCAGTGAAATTTCTGACCAATCTGCTAATTTTTCAC
AATCTTTACTGACAAAAATAAAAGAAAAAATTGACAAAGATGAACAAGTCATTTTGATGTTGAATCGAAGGGGTTATTCT
TCATTTGTCATGTGTCGTGAATGTGGTTTTGTTGTTGATTGTCCCAATTGCGATATTTCCTTGACCCTTCACATGGATAC
CCGAACTTTGAATTGTCATTATTGTGGTCATGTCGAAGGAATTCCGCAAACTTGTCCTAATTGTCATTCACGAAAAATTC
GTTATTACGGTTCAGGAACGCAAAAAGTCGAAGAAGAACTTCGAGAAATCATGCCTGAAGCACGAGTTTTGCGAATGGAT
GTAGATACGACTCGGACAAAAAATGCTCACGAAAAAATTCTTGAAAAGTTTGGTAATCATGAAGCTGATATCCTCTTAGG
AACTCAGATGATTGCCAAAGGTTTAGATTTTCCAAACGTAACCTTAGTGGGTGTCATTAACGCAGATACTGGCCTAAATC
TACCTGATTTTAGAGCGAGTGAGCGTTCCTTTGAATTGTTGATGCAGGTGGCTGGACGAGCTGGACGAGCAGATAAAAAA
GGTGAAGTATTAATTCAAACTTTCAATCCACATCATTATGCTATTGAAATGACCAAGGCTCAAGATTACGAAAAATTTTA
TGAAATGGAAATGAGATTTCGTCGTGAACTTTCCTATCCACCTTATTATTTCACCGTTCAAGTGCTTGTTTCTCATCAAG
ATGAAGAAGTTGCCATTCAAAAATCTTATGAGATCGCTCAATTACTCAAGCAAAATTTGACAGAAAAAGCTAAAATATTA
GGGCCAACCGCCAGACCAATTGCCAGAACCCATAACCTTTATCATTATCAAATCTTAGTTAAATATCGTTTTGAAGAAGA
ATTAGAATCAGCTTTAAATCAAGTGCTTGAATTAACCCAACTTCGGGATAACAAAAATTTACGAGTATTGATTGATAGTG
AGCCACAAAATTTTGTATAA

Domains

Predicted by InterProScan.

• 3DNA-binding domain (3BD) (PriA_3primeBD) (5-107)

• Type III restriction enzyme, res subunit (ResIII) (247-405)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (494-518)

• Helicase conserved C-terminal domain (Helicase_C) (561-637)

• Primosomal protein N C-terminal domain (PriA_C) (681-775)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Bacillus subtilis subsp. subtilis str. 168	47.512	100	0.49