Detailed information    

insolico Bioinformatically predicted

Overview

Name   mutS/mutS2   Type   Machinery gene
Locus tag   MWH27_RS14645 Genome accession   NZ_JALJVV010000001
Coordinates   2707360..2709717 (-) Length   785 a.a.
NCBI ID   WP_014480503.1    Uniprot ID   -
Organism   Bacillus subtilis strain A-5     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2702360..2714717
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MWH27_RS14620 (MWH27_14595) etfB 2702801..2703574 (-) 774 WP_014480500.1 electron transfer flavoprotein subunit beta -
  MWH27_RS14625 (MWH27_14600) fadB 2703589..2704365 (-) 777 WP_014480501.1 enoyl-CoA hydratase -
  MWH27_RS14630 (MWH27_14605) fadR 2704380..2704964 (-) 585 WP_014477593.1 fatty acid metabolism transcriptional regulator FadR -
  MWH27_RS14635 (MWH27_14610) lcfA 2705070..2706752 (-) 1683 WP_014480502.1 long-chain-fatty-acid--CoA ligase LcfA -
  MWH27_RS14640 (MWH27_14615) yshE 2706941..2707345 (-) 405 WP_003237674.1 DUF350 domain-containing protein -
  MWH27_RS14645 (MWH27_14620) mutS/mutS2 2707360..2709717 (-) 2358 WP_014480503.1 endonuclease MutS2 Machinery gene
  MWH27_RS14650 (MWH27_14625) polX 2709738..2711450 (-) 1713 WP_014480504.1 DNA polymerase/3'-5' exonuclease PolX -
  MWH27_RS14655 (MWH27_14630) yshB 2711524..2712057 (-) 534 WP_014480505.1 CvpA family protein -
  MWH27_RS14660 (MWH27_14635) zapA 2712064..2712321 (-) 258 WP_003229534.1 cell division protein ZapA -
  MWH27_RS14665 (MWH27_14640) rnhC 2712455..2713393 (+) 939 WP_014480506.1 ribonuclease HIII -

Sequence

Protein


Download         Length: 785 a.a.        Molecular weight: 87399.77 Da        Isoelectric Point: 6.1361

>NTDB_id=990425 MWH27_RS14645 WP_014480503.1 2707360..2709717(-) (mutS/mutS2) [Bacillus subtilis strain A-5]
MQQKVLSALEFHKVKEQVIGHAASSLGKEMLLELKPSASIDEIKKQLDEVDEASDIIRLRGQAPFGGLVDIRGALRRAEI
GSVLSPSEFTEISGLLYAVKQMKHFITQMAEDGVDIPLIHQHAEQLITLSDLERDINSCIDDHGEVLDHASETLRGIRTQ
LRTLESRVRDRLESMLRSSSASKMLSDTIVTIRNDRFVIPVKQEYRSSYGGIVHDTSSSGATLFIEPQAIVDMNNSLQQA
KVKEKQEIERILRVLTEKTAEYTEELFLDLQVLQTLDFIFAKARYAKAVKATKPIMNDTGFIRLKKARHPLLPPDQVVAN
DIELGRDFSTIVITGPNTGGKTVTLKTLGLLTLMAQSGLHIPADEGSEAAVFEHVFADIGDEQSIEQSLSTFSSHMVNIV
GILEQVNENSLVLFDELGAGTDPQEGAALAMSILDDVHRTNARVLATTHYPELKAYGYNREGVMNASVEFDIETLSPTYK
LLIGVPGRSNAFEISKRLGLPDHIIGQAKSEMTAEHNEVDTMIASLEQSKKRAEEELSETESIRKEAEKLHKELQQQIIE
LNSKKDKMLEEAEQQAAEKVKAAMKEAEDIIHELRTIKEEHKSFKDHELINAKKRLEGAMPAFEKSKKPEKPKAQKRDFK
PGDEVKVLTFGQKGTLLEKTGGNEWNVQIGILKMKVKEKDLEFIKSAPEQKKEKIITAVKGKDYHVSLELDLRGERYENA
LSRVEKYLDDAVLAGYPRVSIIHGKGTGALRKGVQDLLKNHRSVKSSRFGEAGEGGSGVTVVELK

Nucleotide


Download         Length: 2358 bp        

>NTDB_id=990425 MWH27_RS14645 WP_014480503.1 2707360..2709717(-) (mutS/mutS2) [Bacillus subtilis strain A-5]
GTGCAGCAAAAAGTATTATCAGCTCTTGAATTTCATAAAGTGAAAGAACAGGTTATTGGGCATGCCGCTTCATCGCTCGG
AAAAGAAATGCTTCTCGAGCTTAAGCCTTCTGCTTCTATAGACGAAATTAAAAAACAGCTGGATGAAGTAGACGAAGCTT
CTGACATTATCCGGCTGAGAGGCCAAGCGCCATTTGGCGGCCTTGTAGATATCAGAGGAGCGTTAAGACGGGCGGAAATC
GGCAGCGTTCTCAGTCCTTCTGAATTCACTGAAATCTCAGGCCTGCTTTATGCAGTTAAACAAATGAAACATTTTATCAC
CCAAATGGCTGAAGACGGTGTCGACATTCCGCTGATCCATCAGCATGCTGAACAGCTTATCACGCTGTCCGATTTAGAGC
GGGACATTAATTCCTGCATTGATGATCACGGAGAAGTGCTTGATCATGCATCGGAAACATTAAGAGGAATCCGCACACAG
CTCAGAACACTCGAATCAAGAGTCAGAGACCGGTTAGAGTCGATGCTGCGTTCCTCTTCCGCTTCGAAAATGCTGTCTGA
TACGATTGTTACGATTCGGAATGACCGCTTTGTGATCCCGGTCAAACAGGAGTACAGATCCAGCTATGGAGGAATTGTGC
ACGACACCTCATCCTCTGGTGCGACATTATTCATTGAACCGCAGGCGATTGTAGATATGAACAATTCCCTTCAGCAGGCG
AAAGTGAAAGAAAAGCAAGAAATTGAACGGATTTTGCGTGTGCTGACAGAGAAAACGGCAGAGTATACAGAGGAGCTATT
TCTAGATTTGCAAGTGCTGCAGACGCTTGACTTTATTTTTGCAAAAGCTAGATATGCAAAAGCGGTTAAAGCGACAAAAC
CGATTATGAACGACACCGGCTTTATCCGTTTGAAAAAAGCCCGCCATCCATTGCTTCCGCCTGACCAGGTTGTTGCCAAT
GACATCGAGCTTGGCCGCGATTTTTCAACCATTGTCATCACAGGGCCAAACACCGGGGGGAAAACAGTCACCCTTAAAAC
GTTAGGCCTGCTAACCTTAATGGCGCAATCAGGTCTTCATATCCCGGCAGATGAAGGGTCAGAAGCGGCAGTATTTGAGC
ACGTATTCGCTGATATCGGTGATGAACAGTCGATTGAGCAAAGTTTAAGTACGTTCTCATCCCATATGGTGAACATTGTC
GGCATTTTAGAACAGGTCAATGAAAACAGCCTTGTGCTTTTCGATGAACTTGGTGCAGGGACAGATCCGCAGGAGGGGGC
GGCCCTCGCCATGAGCATCTTGGATGACGTGCATCGCACCAATGCACGAGTGTTAGCTACGACGCATTATCCGGAATTGA
AGGCGTACGGCTATAACAGAGAAGGCGTCATGAATGCCAGCGTTGAATTTGACATCGAAACGCTGTCACCGACCTATAAA
CTTTTAATTGGTGTGCCGGGTCGAAGCAATGCTTTCGAAATTTCAAAACGCCTCGGGCTTCCGGACCATATCATCGGGCA
GGCGAAGTCAGAAATGACGGCCGAGCATAACGAAGTCGATACGATGATTGCGTCGCTGGAACAAAGCAAAAAACGTGCGG
AAGAAGAGCTTTCTGAGACAGAATCAATCAGAAAAGAAGCGGAAAAACTGCATAAAGAGCTGCAGCAGCAAATCATCGAG
CTTAACAGCAAAAAAGACAAAATGCTTGAAGAGGCAGAACAGCAGGCTGCTGAAAAAGTAAAAGCGGCAATGAAAGAAGC
CGAGGACATTATTCATGAATTGCGCACCATAAAAGAAGAACACAAATCCTTCAAGGATCACGAGCTGATTAACGCGAAGA
AACGGTTAGAAGGCGCTATGCCGGCTTTTGAAAAGTCCAAGAAACCGGAAAAACCGAAAGCGCAAAAACGCGACTTTAAG
CCTGGCGACGAGGTGAAAGTCCTCACTTTCGGGCAAAAAGGAACATTGCTCGAAAAAACAGGCGGCAATGAATGGAATGT
TCAAATCGGTATTTTAAAGATGAAAGTAAAAGAAAAAGATCTGGAGTTTATCAAATCAGCTCCGGAGCAAAAAAAAGAAA
AAATCATTACAGCGGTCAAGGGAAAGGACTATCACGTATCGCTTGAACTTGATCTCCGCGGCGAACGCTATGAAAATGCC
CTCAGCCGGGTTGAAAAATACTTGGATGATGCGGTGTTAGCCGGATATCCAAGAGTGTCAATCATCCACGGAAAAGGAAC
CGGCGCTTTAAGAAAAGGCGTACAGGATCTTCTGAAAAACCACCGCAGCGTCAAAAGTTCCCGTTTCGGTGAAGCAGGTG
AGGGAGGATCAGGCGTTACGGTTGTTGAACTAAAATAA

Domains

Predicted by InterProScan.

Smr domain (Smr)

(710-785)

MutS2 and Smr-associated SH3 domain (MSSS)

(641-681)

MutS domain V (MutS_V)

(331-512)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  mutS/mutS2 Bacillus subtilis subsp. subtilis str. 168

99.618

100

0.996

Multiple sequence alignment