Detailed information NTG0989710

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: AAHX58_RS21715
• Genome accession: NZ_CP152571
• Coordinates: 4413647..4414447 (+)
• Length: 266 a.a.
• NCBI ID: WP_008807384.1
• Uniprot ID: A0A0J4LMF2
• Organism: Klebsiella variicola subsp. variicola strain NK_H8_045
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4408647..4419447

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AAHX58_RS21680 (AAHX58_21675)	gspH	4408800..4409315 (+)	516	WP_008807378.1	type II secretion system minor pseudopilin GspH	-
AAHX58_RS21685 (AAHX58_21680)	gspI	4409312..4409677 (+)	366	WP_004894160.1	type II secretion system minor pseudopilin GspI	-
AAHX58_RS21690 (AAHX58_21685)	gspJ	4409674..4410270 (+)	597	WP_032731630.1	type II secretion system minor pseudopilin GspJ	-
AAHX58_RS21695 (AAHX58_21690)	gspK	4410267..4411247 (+)	981	WP_373890536.1	type II secretion system minor pseudopilin GspK	-
AAHX58_RS21700 (AAHX58_21695)	gspL	4411240..4412436 (+)	1197	WP_043875784.1	type II secretion system protein GspL	-
AAHX58_RS21705 (AAHX58_21700)	-	4412429..4412914 (+)	486	WP_012968921.1	type II secretion system protein M	-
AAHX58_RS21710 (AAHX58_21705)	-	4412904..4413650 (+)	747	WP_110202065.1	type II secretion system protein N	-
AAHX58_RS21715 (AAHX58_21710)	pilD	4413647..4414447 (+)	801	WP_008807384.1	A24 family peptidase	Machinery gene
AAHX58_RS21720 (AAHX58_21715)	hrpB	4414494..4416923 (-)	2430	WP_032740432.1	ATP-dependent helicase HrpB	-
AAHX58_RS21725 (AAHX58_21720)	thpR	4416996..4417532 (+)	537	WP_008807387.1	RNA 2',3'-cyclic phosphodiesterase	-
AAHX58_RS21730 (AAHX58_21725)	sfsA	4417532..4418248 (+)	717	WP_008807388.1	DNA/RNA nuclease SfsA	-
AAHX58_RS21735 (AAHX58_21730)	dksA	4418411..4418866 (+)	456	WP_007749299.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29433.14 Da
• Isoelectric Point: 8.7151

>NTDB_id=989710 AAHX58_RS21715 WP_008807384.1 4413647..4414447(+) (pilD) [Klebsiella variicola subsp. variicola strain NK_H8_045]
MTTLTALSLHFPLVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLLLLSFLLMLARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASACGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=989710 AAHX58_RS21715 WP_008807384.1 4413647..4414447(+) (pilD) [Klebsiella variicola subsp. variicola strain NK_H8_045]
ATGACGACATTAACGGCATTATCGCTGCACTTTCCCCTTGTCTGGTATGGCTTCCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAACGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCCTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGGGCGCTGCTCGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGCTGCTGCTCTCGTTCCTGCTGATGCTGGCGC
GGATTGACGCCCGGACCCAGCTGTTGCCCGACCGTCTGACGCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGACGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCCTCGGCCTGCGGGCTGGTGTGGACCCTGCTGCAGCGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGAGGGGGCATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

3D structure

• AlphaFold DB: A0A0J4LMF2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	45.455	100	0.47
pilD	Acinetobacter nosocomialis M2	45.627	98.872	0.451
pilD	Acinetobacter baumannii D1279779	44.867	98.872	0.444
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425