Detailed information NTG0989378

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: HMPREF9319_RS01820
• Genome accession: NZ_GL397128
• Coordinates: 366759..369149 (+)
• Length: 796 a.a.
• NCBI ID: WP_003063526.1
• Uniprot ID: E0PBZ0
• Organism: Streptococcus equinus ATCC 700338
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 361759..374149

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HMPREF9319_RS01795 (HMPREF9319_0358)	-	362343..363107 (+)	765	WP_003063521.1	(S)-acetoin forming diacetyl reductase	-
HMPREF9319_RS01800 (HMPREF9319_0359)	-	363186..363671 (-)	486	WP_003063522.1	S-ribosylhomocysteine lyase	-
HMPREF9319_RS01805 (HMPREF9319_0360)	-	363822..365429 (+)	1608	WP_003063523.1	ribonuclease Y	-
HMPREF9319_RS01810 (HMPREF9319_0361)	gmk	365683..366300 (+)	618	WP_003063524.1	guanylate kinase	-
HMPREF9319_RS01815 (HMPREF9319_0362)	rpoZ	366323..366637 (+)	315	WP_013851522.1	DNA-directed RNA polymerase subunit omega	-
HMPREF9319_RS01820 (HMPREF9319_0363)	priA	366759..369149 (+)	2391	WP_003063526.1	primosomal protein N'	Machinery gene
HMPREF9319_RS01825 (HMPREF9319_0364)	fmt	369210..370145 (+)	936	WP_003063528.1	methionyl-tRNA formyltransferase	-
HMPREF9319_RS01830 (HMPREF9319_0365)	rsmB	370135..371457 (+)	1323	WP_003063529.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
HMPREF9319_RS01835 (HMPREF9319_0366)	-	371495..372232 (+)	738	WP_003063530.1	Stp1/IreP family PP2C-type Ser/Thr phosphatase	-
HMPREF9319_RS01840 (HMPREF9319_0367)	pknB	372232..374112 (+)	1881	WP_003063531.1	Stk1 family PASTA domain-containing Ser/Thr kinase	Regulator

Sequence

Protein

• Length: 796 a.a.
• Molecular weight: 91067.31 Da
• Isoelectric Point: 7.2994

>NTDB_id=989378 HMPREF9319_RS01820 WP_003063526.1 366759..369149(+) (priA) [Streptococcus equinus ATCC 700338]
MVKIAQVIVDVPLMQTDKPFSYIIPKDVEDQVTIGSRVHVPFGRGNRLLQGFVVGFSDTFDNTVTDLKAISEVLDFEPVL
NAEQLELAEQMRHTVFSYKISILKSMIPNLLNSQYDKRLTPTESLSSEERLALFGEKESRLYSSFTEEEAKKVARLVQAG
KITVDYLAKDKKNIKTEKYYHIQAKKMAAADISNRAKKRLELRDYLLENPEEGRLSDLHHLFSRDVVKFFVDNQLITVLE
REKKRSDAYFDVATTDFLDLNAEQAAVVEQVTSQIAQESNPFLLEGVTGSGKTEVYLHIIDKVLKLGKTAIVLVPEISLT
PQMTNRFISRFGKQVAIMHSALSDGEKFDEWRKIKSGQARVVVGARSAIFVPIENIGAIIIDEEHEATYKQESNPRYHAR
DVALLRAKYHKAVLLMGSATPSIESRARASRGVYQFLQLTHRANPMAKIPKVEIVDFRDYVGQQEVSNFTPYLLEKIADR
LEKNEQVVLMLNRRGYSSFIMCRDCGYVDECPNCDISLTLHMDTKTMNCHYCGFEKSIPHTCPNCHSRSIRYYGTGTQKA
YDELVEVFPQARILRMDVDTTRQKGAHQRILDKFGNHEADILLGTQMIAKGLDFPNVTLVGVLNADTSLNLPDFRSSERT
FQLLTQVAGRAGRAEKEGEVLIQTYNPQHYAIQLAQKQDYEAFYAYEMGIRRQLAYPPYYFTVGLTLSHKDEQTVVRKSF
ELLQLLRQQLSDKIKILGPTPKPIARTHNLYHYQIIVKYRFEDNLENVLNQILDLTQLPENKDLRLVIDYEPQNFM

Nucleotide

• Length: 2391 bp

>NTDB_id=989378 HMPREF9319_RS01820 WP_003063526.1 366759..369149(+) (priA) [Streptococcus equinus ATCC 700338]
ATGGTAAAAATTGCTCAGGTCATCGTTGATGTCCCACTTATGCAAACAGATAAACCTTTCTCTTACATCATTCCGAAAGA
TGTTGAAGACCAAGTGACCATCGGCTCACGAGTTCATGTACCTTTTGGACGTGGTAATCGGCTTTTGCAAGGCTTTGTTG
TCGGTTTTTCTGATACTTTTGATAATACTGTGACTGACTTAAAAGCTATTAGTGAAGTTTTGGATTTTGAGCCTGTGTTG
AATGCAGAACAGCTAGAATTGGCAGAGCAGATGCGTCATACCGTGTTTTCTTATAAAATTTCCATTCTGAAATCGATGAT
TCCTAATCTGCTGAATTCGCAGTATGATAAACGTTTAACGCCTACAGAAAGCTTATCTAGCGAAGAACGTTTGGCATTGT
TTGGTGAGAAAGAGAGCCGACTTTATTCGTCATTCACTGAGGAAGAAGCGAAAAAAGTCGCTCGCTTGGTTCAAGCAGGA
AAAATTACCGTTGATTATTTAGCTAAAGATAAGAAAAATATCAAAACAGAAAAATATTATCACATTCAAGCTAAAAAAAT
GGCTGCCGCAGACATCTCCAATCGTGCTAAAAAAAGATTGGAATTACGTGATTATTTGTTAGAGAATCCTGAAGAAGGAA
GACTGTCAGATTTGCATCATCTTTTTTCACGTGATGTTGTCAAATTTTTTGTGGATAATCAGTTGATAACTGTTCTTGAA
AGAGAGAAAAAACGTTCAGACGCTTATTTTGATGTAGCAACAACAGATTTTCTTGATTTAAATGCTGAACAAGCGGCTGT
CGTTGAACAAGTAACTAGCCAAATTGCTCAGGAAAGTAATCCGTTTTTACTGGAAGGTGTGACAGGTTCAGGAAAAACTG
AAGTTTATCTGCATATCATTGACAAGGTTTTGAAACTCGGTAAAACAGCCATTGTCTTGGTGCCTGAAATTTCTTTGACA
CCACAAATGACCAATCGTTTTATTTCACGTTTCGGCAAGCAAGTGGCTATCATGCACTCTGCTTTGTCAGACGGTGAAAA
GTTTGACGAATGGCGAAAAATCAAGTCTGGACAAGCACGTGTCGTTGTTGGGGCACGCTCTGCCATTTTTGTTCCTATTG
AAAATATTGGTGCCATTATCATTGACGAGGAACATGAGGCGACTTACAAGCAAGAATCCAATCCGCGCTACCATGCGCGT
GATGTGGCTTTGCTTCGTGCTAAATACCATAAAGCTGTGTTGTTAATGGGCTCTGCAACACCAAGTATCGAAAGCCGTGC
TCGGGCTAGTCGTGGTGTTTACCAATTCCTACAATTGACACATCGTGCTAATCCTATGGCAAAGATTCCAAAAGTTGAAA
TTGTCGATTTTCGCGATTATGTTGGGCAGCAAGAGGTTAGCAACTTTACTCCGTATCTCTTAGAAAAAATTGCTGACCGC
TTAGAAAAAAATGAGCAAGTCGTGCTCATGCTCAATCGTCGTGGTTACTCTAGTTTTATCATGTGCCGTGATTGTGGCTA
TGTTGATGAATGCCCAAATTGCGACATTTCCTTGACGCTGCATATGGATACCAAAACCATGAATTGTCATTATTGTGGTT
TTGAAAAATCCATTCCACACACTTGTCCGAATTGTCATAGCCGTAGTATTCGGTATTATGGAACAGGGACACAGAAGGCT
TATGATGAACTCGTGGAAGTTTTTCCACAAGCTCGTATTTTACGAATGGACGTTGATACCACACGTCAAAAAGGGGCTCA
TCAACGCATTTTGGATAAATTTGGCAACCATGAAGCCGATATTTTACTTGGCACACAAATGATTGCTAAGGGACTTGATT
TTCCAAATGTTACTTTGGTAGGAGTTTTAAATGCGGATACGTCTTTAAATTTACCTGATTTTCGTTCTTCTGAACGCACA
TTCCAATTGTTGACACAAGTTGCAGGGCGTGCAGGACGTGCAGAAAAAGAAGGTGAGGTTCTTATCCAGACCTACAATCC
ACAGCACTATGCTATTCAACTAGCGCAAAAGCAAGACTACGAGGCATTTTACGCTTATGAAATGGGAATCCGTAGGCAAT
TAGCTTATCCGCCTTATTATTTCACGGTCGGATTGACCTTATCACATAAAGATGAGCAGACCGTTGTCCGTAAATCATTT
GAACTCTTGCAATTATTGCGCCAGCAATTATCAGATAAAATCAAAATTCTCGGTCCGACACCAAAACCGATTGCCAGAAC
GCATAATCTTTATCATTATCAAATTATCGTTAAATATCGCTTTGAGGACAATTTAGAGAATGTCTTGAACCAGATTTTAG
ATTTGACACAATTACCAGAAAATAAAGATTTACGCTTAGTCATTGACTATGAGCCACAAAATTTCATGTAG

Domains

Predicted by InterProScan.

• Primosomal protein N C-terminal domain (PriA_C) (698-792)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (6-108)

• Helicase conserved C-terminal domain (Helicase_C) (572-654)

• Type III restriction enzyme, res subunit (ResIII) (259-422)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (511-536)

3D structure

• AlphaFold DB: E0PBZ0

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Bacillus subtilis subsp. subtilis str. 168	47.095	100	0.479