Detailed information    

insolico Bioinformatically predicted

Overview

Name   endA   Type   Machinery gene
Locus tag   R8624_RS08940 Genome accession   NZ_AP026930
Coordinates   1744665..1745489 (-) Length   274 a.a.
NCBI ID   WP_050102589.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain PZ900701208     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1739665..1750489
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R8624_RS08935 (PC1195_17480) - 1743043..1744374 (-) 1332 WP_000390043.1 hemolysin family protein -
  R8624_RS08940 (PC1195_17490) endA 1744665..1745489 (-) 825 WP_050102589.1 DNA-entry nuclease EndA Machinery gene
  R8624_RS08945 (PC1195_17500) - 1745529..1745717 (-) 189 WP_001812859.1 DNA-directed RNA polymerase subunit beta -
  R8624_RS08950 (PC1195_17510) murA 1745710..1746993 (-) 1284 WP_000358027.1 UDP-N-acetylglucosamine 1-carboxyvinyltransferase -
  R8624_RS08955 (PC1195_17520) - 1747017..1747247 (-) 231 WP_000250392.1 DUF1146 family protein -
  R8624_RS08960 (PC1195_17530) sepM 1747305..1748342 (-) 1038 WP_000727277.1 SepM family pheromone-processing serine protease Regulator
  R8624_RS08965 (PC1195_17540) coaD 1748326..1748814 (-) 489 WP_001280769.1 pantetheine-phosphate adenylyltransferase -
  R8624_RS08970 (PC1195_17550) rsmD 1748804..1749343 (-) 540 WP_000706963.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  R8624_RS08975 (PC1195_17560) asnA 1749408..1750400 (-) 993 WP_000747993.1 aspartate--ammonia ligase -

Sequence

Protein


Download         Length: 274 a.a.        Molecular weight: 29936.60 Da        Isoelectric Point: 10.0547

>NTDB_id=98767 R8624_RS08940 WP_050102589.1 1744665..1745489(-) (endA) [Streptococcus pneumoniae strain PZ900701208]
MNKKTRQTLIGLLVLLLLSTGSYYIKQMQSAPNSPKTKLSQKKQASEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIV
NGNKTNLDAKVSSKPYADNKTKTVGKETVPTVANALLSKATRQYKNREETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRVRYRVTLYYASNEDLVPSASQIE
AKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ

Nucleotide


Download         Length: 825 bp        

>NTDB_id=98767 R8624_RS08940 WP_050102589.1 1744665..1745489(-) (endA) [Streptococcus pneumoniae strain PZ900701208]
ATGAACAAAAAAACAAGACAGACACTAATCGGACTGCTAGTGTTATTGCTTTTGTCTACAGGGAGCTATTATATCAAGCA
GATGCAGTCGGCACCTAATAGTCCTAAAACCAAGCTTAGTCAGAAAAAACAAGCGTCTGAAGCTCCTAGTCAAGCATTGG
CAGAGAGTGTCTTAACAGACGCAGTCAAGAGTCAAATAAAGGGGAGTCTGGAGTGGAATGGCTCAGGTGCTTTTATCGTC
AATGGTAATAAAACAAATCTAGATGCCAAGGTTTCAAGTAAGCCCTACGCTGACAATAAAACAAAGACAGTGGGCAAGGA
AACTGTTCCAACCGTAGCTAATGCCCTCTTGTCTAAGGCCACTCGTCAGTACAAGAATCGTGAAGAAACTGGGAATGGTT
CAACTTCTTGGACTCCTCCAGGTTGGCATCAGGTCAAGAATCTAAAGGGCTCTTATACCCATGCAGTCGATAGAGGTCAT
TTGTTAGGCTATGCCTTAATCGGTGGTTTGGATGGTTTTGATGCCTCAACAAGCAATCCTAAAAACATTGCTGTTCAGAC
AGCCTGGGCAAATCAGGCACAAGCCGAGTATTCGACTGGTCAAAACTACTATGAAAGCAAGGTGCGTAAAGCCTTGGACC
AAAACAAGCGTGTCCGTTACCGTGTAACCCTTTACTACGCTTCAAACGAGGATTTAGTTCCCTCAGCTTCACAGATTGAA
GCCAAGTCTTCGGATGGAGAATTGGAATTCAATGTTCTAGTTCCCAATGTTCAAAAGGGACTTCAACTGGATTACCGAAC
TGGAGAAGTAACTGTAACTCAGTAA

Domains

Predicted by InterproScan.

DNA/RNA non-specific endonuclease (Endonuclease_NS)

(75-247)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  endA Streptococcus pneumoniae Rx1

98.905

100

0.989
  endA Streptococcus pneumoniae D39

98.905

100

0.989
  endA Streptococcus pneumoniae R6

98.905

100

0.989
  endA Streptococcus pneumoniae TIGR4

98.905

100

0.989

Multiple sequence alignment