Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   MHB67_RS05150 Genome accession   NZ_CP152051
Coordinates   1067317..1070826 (+) Length   1169 a.a.
NCBI ID   WP_342492834.1    Uniprot ID   -
Organism   Bacillus sp. FSL H8-0516     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1062317..1075826
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHB67_RS05120 (MHB67_05120) lepB 1062835..1063347 (+) 513 WP_044335799.1 signal peptidase I -
  MHB67_RS05125 (MHB67_05125) - 1063386..1064531 (-) 1146 WP_151039543.1 AbrB family transcriptional regulator -
  MHB67_RS05130 (MHB67_05130) - 1064798..1065220 (+) 423 WP_024425495.1 BlaI/MecI/CopY family transcriptional regulator -
  MHB67_RS05135 (MHB67_05135) - 1065213..1066052 (+) 840 WP_024425496.1 M56 family metallopeptidase -
  MHB67_RS05140 (MHB67_05140) - 1066110..1066520 (+) 411 WP_024425497.1 DoxX family protein -
  MHB67_RS05145 (MHB67_05145) - 1066718..1067164 (-) 447 WP_044334155.1 ferritin-like domain-containing protein -
  MHB67_RS05150 (MHB67_05150) addB 1067317..1070826 (+) 3510 WP_342492834.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  MHB67_RS05155 (MHB67_05155) addA 1070804..1074508 (+) 3705 WP_342492835.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  MHB67_RS05160 (MHB67_05160) sbcD 1074535..1075710 (+) 1176 WP_122631156.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1169 a.a.        Molecular weight: 135051.20 Da        Isoelectric Point: 5.3539

>NTDB_id=986908 MHB67_RS05150 WP_342492834.1 1067317..1070826(+) (addB) [Bacillus sp. FSL H8-0516]
MEIQFLAGRSGSGKTTAILEEIKEQLRLDPLGPPIIFLVPDQMTFLMEYELAKTSEAGGMIRAKVFSFTRLAWSILQQTG
GANRQFVTSTGIQMLLRKVIEEEKEKFKVFKKASDKPGFVEEIEKTMAEFKRYCMLPEDIEKISLSSMHSEYTEERRAAE
KLHDLHVLYQQMEQHLQDEYVHSEDYLTLLTQQIPLSEEIKGAHIYIDGFYQFTPQQLLVIEQLLLHAEKITAAFTVDRS
YHDKLPNELDLFRMTGKTYFQLYQLAKECGAAISEHILERNNRHLHTPDLAYLERQYEQRPVQPYQEVTPHLTVSKSASK
RAEIEGVAREILHLVREEGLRLRDISIVARHVDDYKDTLKEVFRDYDIPFFIDGNESMQYHPLIELIRSSMDVIKGNWRY
EAVFRCVKTEFLFPLELARNKAREQADQLENYCIAYGVKGERWTNGSRFHYRRFQSLDEDFGQTDQEIEMEQMLNDVKEW
IAPPLFQLQKRLKKAQKVKDMVEAVYVFLEEIQVPDKLEKARLEAEEAGRLAEAMQHGQVWEAVIQLMDEFVEMLGEEEL
SFPLFQQMMDTGLASLKFALIPPSLDQVFIGSMDLSRMYQVKCMFIIGVNDGVIPARPSDESVLSEDDREWLKRAGAELA
ETGKERLLDEQFLIYQALSSPSHHLYLSYSVSDEEGRSLLPSTLIKYCQELMPNHEHALYVLDPEQLDDKEQLKFVANEH
VSLSYTVSQLQQWLNQYPISGVWWSVYNYLMTSSHRDVSKNILSSLFFTNRAKRLKPNVTKELYGDHIQGSVSRMEKFNE
CAFSHFASHGLKLKDRQFYKLEAPDIGQLFHSALKHISDTLVEQKKDWKNLTKDDCVNYSRHAIEQLAPRLQKEILLSSN
RHAYIKEKLQKILIRVSTILSEHARVSGFSPVGLELGFGGQGPLPPFTFQLKNGCTMELVGRIDRVDKAEGSKGLLLRIV
DYKSSEKGLDLAEVYYGLALQMLTYLDLTITYSKEWLGVEATPAGILYFHIHDPLIQVPIPLAEDEIEQEIFKKFKMKGL
LLEDVEAVKLMDQTLESGRSQVIQAGLKKDGSFRSDSAVLSEDHFHILTQHVRRTFEEAGERITNGEVAINPYKLKDQTP
CRFCSFKSICQFDESIEDNEFRVLSSEKDDVVIDRIKKEGDQYANTKTE

Nucleotide


Download         Length: 3510 bp        

>NTDB_id=986908 MHB67_RS05150 WP_342492834.1 1067317..1070826(+) (addB) [Bacillus sp. FSL H8-0516]
GTGGAGATTCAGTTTTTAGCAGGCAGATCGGGGAGTGGAAAAACGACTGCAATCTTAGAGGAAATCAAAGAACAGCTTCG
GCTTGATCCGTTGGGTCCGCCAATCATTTTTTTAGTCCCGGATCAAATGACATTCTTAATGGAATACGAGCTTGCAAAAA
CGTCTGAAGCTGGCGGGATGATCAGGGCAAAAGTATTTAGTTTCACGCGTCTTGCTTGGTCTATTTTACAGCAGACTGGC
GGAGCGAACCGGCAATTTGTGACAAGCACAGGAATTCAAATGCTTTTAAGAAAAGTGATTGAAGAGGAGAAAGAAAAGTT
TAAGGTATTCAAAAAAGCGAGTGATAAGCCGGGGTTTGTGGAGGAAATCGAAAAGACGATGGCTGAGTTCAAGAGATACT
GCATGCTGCCGGAGGATATTGAAAAAATTTCATTATCGAGCATGCATTCAGAGTATACAGAAGAAAGACGGGCAGCCGAG
AAATTGCATGATCTGCATGTTCTTTATCAGCAGATGGAGCAGCATTTACAAGATGAATATGTGCATTCAGAAGACTATTT
GACACTTCTCACGCAGCAAATTCCTTTATCTGAAGAAATAAAAGGAGCACACATTTATATAGATGGTTTTTATCAATTTA
CACCGCAGCAGCTTCTCGTCATTGAACAGCTTCTGCTGCATGCTGAGAAAATAACGGCTGCTTTCACAGTGGATCGTTCC
TATCATGATAAGCTGCCAAATGAACTTGATTTATTTCGTATGACAGGTAAAACGTATTTCCAGCTCTATCAGCTTGCCAA
AGAGTGCGGAGCGGCCATTTCTGAGCACATTTTGGAGAGAAATAACCGTCATCTTCATACGCCGGATCTTGCTTATTTAG
AACGGCAATATGAGCAGAGACCGGTGCAGCCATATCAGGAAGTCACTCCTCATCTCACAGTATCTAAAAGTGCGAGTAAA
CGAGCTGAAATTGAGGGAGTAGCGAGAGAAATTCTTCATTTAGTGAGAGAAGAAGGACTTAGACTGCGAGATATTTCGAT
TGTAGCGCGGCATGTAGACGACTATAAAGATACGTTAAAAGAGGTTTTTCGAGATTACGATATTCCTTTTTTTATTGATG
GAAATGAATCGATGCAGTATCATCCGCTCATTGAATTGATTCGTTCGAGCATGGATGTCATAAAAGGAAACTGGCGGTAT
GAGGCCGTGTTTCGATGCGTGAAGACAGAGTTCTTATTTCCGCTCGAACTTGCCAGAAACAAAGCGAGAGAACAGGCAGA
TCAGCTTGAAAACTACTGTATTGCTTATGGTGTAAAAGGAGAACGCTGGACAAACGGCTCCCGTTTCCATTACAGGCGGT
TCCAATCATTAGATGAGGATTTTGGACAAACAGATCAAGAAATTGAAATGGAACAGATGCTGAATGATGTAAAGGAATGG
ATCGCACCTCCGCTTTTCCAATTGCAAAAAAGGCTGAAAAAAGCGCAAAAGGTAAAAGACATGGTAGAGGCAGTCTATGT
CTTTTTAGAGGAAATACAAGTGCCGGATAAACTTGAAAAGGCTAGGCTGGAAGCTGAAGAAGCAGGTAGATTAGCTGAAG
CCATGCAGCATGGACAAGTATGGGAAGCTGTTATTCAATTAATGGACGAATTTGTGGAAATGCTCGGTGAGGAGGAGCTC
TCATTTCCTTTATTCCAACAGATGATGGATACAGGATTGGCCTCTTTAAAATTCGCTTTAATTCCGCCTTCACTCGACCA
AGTATTTATCGGAAGTATGGATTTATCCAGAATGTATCAAGTGAAGTGTATGTTTATCATTGGGGTAAATGATGGCGTTA
TTCCCGCACGCCCTTCTGACGAAAGTGTATTGTCTGAGGATGACAGGGAATGGTTAAAGCGAGCAGGAGCAGAGCTGGCA
GAGACAGGAAAGGAACGGCTACTAGATGAGCAATTTTTAATTTACCAGGCGCTGTCGAGTCCATCTCATCATTTGTATCT
GTCTTATTCAGTGTCAGATGAAGAGGGGCGCTCTCTATTGCCTTCGACTCTTATCAAGTATTGTCAGGAGCTTATGCCAA
ATCATGAGCATGCTTTGTATGTGTTAGACCCGGAACAGCTGGATGACAAGGAGCAATTAAAATTTGTAGCGAATGAGCAT
GTCTCGTTATCGTATACTGTCTCGCAGCTGCAGCAATGGCTCAACCAATATCCCATCAGTGGTGTATGGTGGAGTGTCTA
TAATTATTTGATGACGTCATCTCATCGGGATGTATCAAAAAACATCTTGTCGAGTTTGTTTTTTACAAATCGAGCGAAGC
GCTTAAAACCAAACGTCACAAAAGAACTTTACGGTGATCACATTCAAGGCAGTGTTTCAAGAATGGAGAAATTCAATGAG
TGTGCATTTTCTCACTTTGCTTCCCACGGGTTAAAACTAAAGGACCGGCAATTTTATAAATTGGAAGCACCTGATATCGG
CCAGCTGTTTCACTCGGCCTTAAAGCATATTTCAGATACGCTTGTTGAACAAAAAAAGGATTGGAAAAACCTTACGAAGG
ATGACTGTGTCAACTATTCAAGACATGCAATCGAACAGCTTGCTCCGCGTCTTCAAAAGGAAATCCTATTAAGCTCTAAC
CGGCATGCTTATATTAAAGAAAAGCTCCAGAAAATCTTGATTCGAGTCTCTACTATATTAAGTGAGCATGCAAGAGTGAG
TGGATTTTCTCCCGTTGGGCTTGAGTTAGGCTTTGGCGGCCAAGGACCACTTCCACCGTTTACTTTTCAATTGAAAAATG
GCTGCACGATGGAGCTTGTCGGAAGAATTGACCGGGTGGATAAAGCAGAAGGTTCAAAAGGGCTGCTGTTGAGAATCGTC
GACTACAAATCGAGTGAGAAAGGTCTTGATCTAGCAGAAGTATACTATGGGCTGGCTTTGCAAATGCTCACATACTTAGA
CCTGACCATTACGTATTCGAAGGAGTGGCTCGGTGTAGAAGCAACACCAGCTGGCATTTTATATTTCCATATTCATGATC
CGCTCATTCAAGTACCGATTCCGCTTGCAGAGGATGAAATTGAGCAGGAAATCTTCAAGAAATTTAAAATGAAAGGTCTA
TTGCTTGAAGATGTAGAAGCGGTAAAGCTAATGGATCAAACGCTTGAATCAGGAAGATCACAAGTCATTCAAGCCGGCTT
GAAAAAGGATGGGTCTTTCCGTTCTGACTCTGCTGTTTTAAGTGAAGATCATTTCCATATACTCACACAGCATGTTCGGC
GCACGTTTGAAGAGGCAGGCGAAAGAATTACAAATGGCGAGGTAGCCATTAATCCATATAAATTGAAGGATCAAACACCA
TGCCGCTTTTGTTCATTTAAGTCTATTTGTCAATTTGATGAATCGATAGAGGATAATGAATTTAGAGTGCTTTCATCTGA
AAAGGATGATGTTGTGATAGACCGGATCAAAAAAGAAGGGGATCAGTATGCAAATACCAAAACCGAATAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

65.547

99.316

0.651

Multiple sequence alignment