Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   MHH29_RS01260 Genome accession   NZ_CP152048
Coordinates   229311..231644 (-) Length   777 a.a.
NCBI ID   WP_058336587.1    Uniprot ID   -
Organism   Bacillus sp. FSL K6-0046     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 224311..236644
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHH29_RS01245 (MHH29_01245) dtd 225960..226403 (-) 444 WP_008342725.1 D-aminoacyl-tRNA deacylase -
  MHH29_RS01250 (MHH29_01250) - 226422..228632 (-) 2211 WP_008342726.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
  MHH29_RS01255 (MHH29_01255) - 228775..229287 (-) 513 WP_008342729.1 adenine phosphoribosyltransferase -
  MHH29_RS01260 (MHH29_01260) recJ 229311..231644 (-) 2334 WP_058336587.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  MHH29_RS01265 (MHH29_01265) - 231726..232082 (-) 357 WP_025207709.1 lipopolysaccharide assembly protein LapA domain-containing protein -
  MHH29_RS01270 (MHH29_01270) - 232182..232670 (-) 489 WP_008342734.1 cation:proton antiporter regulatory subunit -
  MHH29_RS01275 (MHH29_01275) secDF 232871..235099 (-) 2229 WP_048001809.1 protein translocase subunit SecDF -
  MHH29_RS01280 (MHH29_01280) - 235141..235437 (-) 297 WP_003216788.1 post-transcriptional regulator -

Sequence

Protein


Download         Length: 777 a.a.        Molecular weight: 87606.35 Da        Isoelectric Point: 4.8057

>NTDB_id=986772 MHH29_RS01260 WP_058336587.1 229311..231644(-) (recJ) [Bacillus sp. FSL K6-0046]
MLLSKMRWEYEHPSEEKVKSLSENLNISALTASLLVQRGLEEVEEARSFLFDQKAEFHDPFLLKGMKEAVERINKAIEEQ
ESIVIFGDYDADGVTSTSVLLHTLKERSAKVDFYIPDRFKEGYGPNEQAFRYIKEQGASLVITVDTGIAAVNEARIAKEI
GLDLIITDHHEPSEELPEALAIVHPKQPGCEYPFKELAGVGVAFKVAHALLGKLPVELLDLAAIGTIADLVPLHDENRWI
AKKGLMQLRQSRRPGLKALLKEAGATLEEANEETVGFQIAPRLNAVGRIEQADPAVHLLMTEDMDEAEELARFVQELNKE
RQKIVSTITEEAIQMVEETGADQSAIVVAKAGWNPGVVGIVASKLTDTFSRPAIVLGIDEETQMAKGSARSISGFDLFFH
LSKCRDILPHFGGHPMAAGMTLQADDVPLLRERLNQYANETLTEEDFIPIQRVDAVCKVDEMTVQAIEEVGLLSPFGMQN
PKPFIMIEDAQLEDIRTIGANQNHIKMSLKDEDKLLDCVGFHQGKLKEELVPGSQISVVGEVSINEWNNRKKPQLMLKDA
RVDEWQLFDLRGKKDWEKKVSTLDPAKTMVFCFDEETKKQSELVDIPIHLIDAENVSTFDVQEKYVIFADVPADEHLLKL
LLENQQPARIYTVFQRKEDHFMSSFPSRDQFKWFYGFLFKRGSFPIKEQGMELAKHRGWTKDTIIFMTKVFFELGFVKIE
NGVLSIVRDAPKKDLTASSSYTAKQRLMELDQTLTYSSAKELKEWLNSMMSEVSPVL

Nucleotide


Download         Length: 2334 bp        

>NTDB_id=986772 MHH29_RS01260 WP_058336587.1 229311..231644(-) (recJ) [Bacillus sp. FSL K6-0046]
ATGTTATTATCCAAAATGCGTTGGGAATATGAACATCCTAGCGAAGAAAAAGTGAAATCACTCTCTGAAAATCTGAATAT
TTCAGCGCTGACTGCATCACTTCTTGTACAAAGAGGACTCGAAGAAGTAGAAGAAGCGAGGTCTTTTTTATTTGATCAAA
AAGCCGAATTTCATGATCCATTCTTATTAAAAGGAATGAAAGAAGCAGTAGAGCGCATCAACAAAGCGATCGAAGAGCAG
GAATCGATTGTGATCTTCGGAGATTATGATGCAGATGGTGTGACAAGTACTTCTGTACTTTTACATACACTTAAAGAACG
ATCTGCGAAGGTAGACTTTTATATTCCAGATCGATTTAAAGAAGGATATGGGCCAAACGAACAGGCTTTCCGATATATCA
AAGAGCAAGGAGCATCGCTTGTCATCACCGTGGATACAGGGATCGCTGCTGTAAATGAAGCACGTATTGCAAAAGAAATA
GGATTAGACCTGATCATCACAGATCACCATGAACCAAGCGAGGAACTGCCTGAGGCACTTGCGATTGTACATCCTAAGCA
GCCTGGTTGTGAGTATCCGTTTAAAGAGCTGGCGGGTGTCGGTGTTGCGTTTAAAGTGGCACATGCACTCCTCGGTAAAC
TCCCAGTAGAATTATTAGATCTTGCTGCAATCGGTACCATTGCGGATCTTGTCCCGCTGCATGATGAAAATAGATGGATT
GCCAAAAAAGGTCTTATGCAGCTTCGACAGTCTAGACGGCCTGGGTTAAAAGCGTTATTAAAAGAAGCAGGCGCCACTCT
TGAAGAAGCAAATGAGGAAACGGTCGGCTTTCAAATTGCGCCAAGGCTGAATGCTGTTGGACGAATTGAGCAGGCAGATC
CGGCTGTTCATCTTCTCATGACTGAGGATATGGATGAAGCAGAAGAACTCGCTCGTTTTGTACAAGAATTGAACAAAGAA
CGGCAGAAAATTGTGAGTACGATCACGGAAGAAGCCATTCAAATGGTCGAAGAGACAGGTGCTGACCAGTCTGCTATCGT
CGTAGCAAAAGCTGGCTGGAATCCAGGCGTTGTCGGCATTGTTGCCTCAAAATTGACGGATACATTTTCTCGTCCGGCGA
TTGTGCTTGGAATAGACGAAGAAACGCAGATGGCGAAAGGGTCAGCACGAAGTATTTCTGGCTTTGATTTATTTTTCCAT
TTGAGCAAGTGCCGTGACATCCTGCCGCATTTCGGTGGACACCCGATGGCAGCAGGAATGACACTTCAAGCGGATGATGT
TCCTTTACTTAGGGAAAGATTGAATCAATATGCAAATGAAACGTTGACTGAAGAAGATTTTATTCCAATTCAACGGGTCG
ATGCAGTATGTAAAGTGGACGAAATGACGGTGCAAGCCATTGAAGAAGTGGGACTGCTGTCCCCGTTTGGTATGCAGAAT
CCTAAGCCGTTCATTATGATTGAAGACGCCCAGCTTGAAGACATCCGAACGATCGGGGCCAATCAAAATCACATTAAAAT
GTCACTAAAAGACGAAGACAAGCTGCTCGACTGTGTCGGTTTTCACCAAGGCAAACTAAAAGAAGAACTTGTCCCAGGGA
GCCAAATTTCTGTCGTTGGCGAGGTCTCGATTAATGAATGGAATAACCGAAAGAAACCTCAGCTCATGCTAAAAGACGCT
AGAGTCGATGAATGGCAGCTGTTTGATTTACGCGGGAAAAAAGATTGGGAAAAGAAAGTATCAACACTTGATCCAGCAAA
AACGATGGTCTTCTGTTTTGACGAAGAAACAAAAAAGCAATCGGAGCTGGTGGATATTCCGATTCACCTTATTGATGCAG
AAAATGTATCCACGTTTGATGTGCAGGAAAAGTATGTGATCTTTGCCGATGTTCCTGCCGATGAACACTTGCTCAAGCTG
CTTTTAGAAAATCAACAACCAGCAAGAATTTATACGGTGTTTCAGCGGAAAGAAGATCACTTTATGTCCTCATTCCCGAG
CCGTGATCAATTCAAATGGTTTTATGGGTTCTTGTTCAAACGGGGAAGTTTTCCTATTAAAGAACAAGGCATGGAGCTTG
CCAAACATAGAGGTTGGACAAAAGATACAATCATTTTTATGACAAAGGTGTTTTTTGAACTCGGTTTTGTTAAAATAGAG
AATGGTGTGCTGTCAATAGTGCGTGATGCCCCTAAAAAAGATTTAACCGCATCATCGTCTTATACAGCAAAACAACGATT
GATGGAACTCGATCAAACACTAACATATTCCTCAGCCAAAGAGCTGAAGGAATGGCTGAATAGCATGATGAGCGAAGTCT
CACCTGTGCTTTGA

Domains

Predicted by InterproScan.

RecJ OB domain (RecJ_OB)

(453-559)

DHH family (DHH)

(83-226)

DHHA1 domain (DHHA1)

(314-437)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(566-766)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

67.181

100

0.672

Multiple sequence alignment