Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   MHB69_RS12295 Genome accession   NZ_CP152043
Coordinates   2378148..2380472 (-) Length   774 a.a.
NCBI ID   WP_342495599.1    Uniprot ID   -
Organism   Bacillus sp. FSL K6-0994     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2373148..2385472
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHB69_RS12280 (MHB69_12280) dtd 2374798..2375241 (-) 444 WP_034279904.1 D-aminoacyl-tRNA deacylase -
  MHB69_RS12285 (MHB69_12285) - 2375260..2377470 (-) 2211 WP_034279902.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
  MHB69_RS12290 (MHB69_12290) - 2377612..2378124 (-) 513 WP_034279900.1 adenine phosphoribosyltransferase -
  MHB69_RS12295 (MHB69_12295) recJ 2378148..2380472 (-) 2325 WP_342495599.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  MHB69_RS12300 (MHB69_12300) - 2380554..2380904 (-) 351 WP_034279896.1 lipopolysaccharide assembly protein LapA domain-containing protein -
  MHB69_RS12305 (MHB69_12305) - 2381006..2381494 (-) 489 WP_041115109.1 cation:proton antiporter regulatory subunit -
  MHB69_RS12310 (MHB69_12310) secDF 2381681..2383909 (-) 2229 WP_061109412.1 protein translocase subunit SecDF -
  MHB69_RS12315 (MHB69_12315) - 2383951..2384247 (-) 297 WP_003216788.1 post-transcriptional regulator -

Sequence

Protein


Download         Length: 774 a.a.        Molecular weight: 87056.70 Da        Isoelectric Point: 4.9495

>NTDB_id=986556 MHB69_RS12295 WP_342495599.1 2378148..2380472(-) (recJ) [Bacillus sp. FSL K6-0994]
MLLSKMRWEYEHPSEEKVKSLSENLNISALTASLLVKRGIEEVEEARSFLFDQKAEFHDPFLLKGMKEAVERINKAIEEQ
ESIVIFGDYDADGVTSTSVLLHTLKERSAIVDFYIPDRFKEGYGPNEQAFRYIKEQGASLVITVDTGIAAVNEARIAKEI
GLDLIITDHHEPSEELPEALAIVHPKQPGCEYPFKELAGVGVAFKVAHALLGKLPVQLLDLAAIGTIADLVPLHDENRWL
AKKGLVQLRQSNRPGLKALLKEAGATLEEANEETVGFQIAPRLNAVGRIEQADPAVHLLMTEDKDEAEELARFVQELNKE
RQKIVSTITEEAIQMVEETPDDQSAIVVAKAGWNPGVVGIVASKLTDTFSRPAIVLGIDEETQMAKGSARSIPGFDLFFH
LSKCRDILPHFGGHPMAAGMTLRADDVALLRERLNQYANETLTEEDFIPIQRVDAVCKVDELTVEAIEEAGMLSPFGMSN
PKPFIMIEDAKLEDIRTIGANHNHIKMSLKDEDKLLDCVGFHQGKLKEEIVPGSRISVIGEMSVNEWNNRKKPQLMLKDA
RVDEWQLFDLRGKKDWEKKVSTLDSEKSLVVCFDEATKQDVDIPIHHVHAENVSTFDVQGKYVVFADVPADEQLLKQLLE
NQKPARIYTVFQRKEDHFMSSFPSRDQFKWFYGFLFKRGSFPIKEQGMDLAKHRGWTKDTIIFMTKVFFELGFVKIENGV
LSIVLDAPKKDLTASASYTAKQRLMELDQTLTYSSAKELKEWLNSMMSEVSPVL

Nucleotide


Download         Length: 2325 bp        

>NTDB_id=986556 MHB69_RS12295 WP_342495599.1 2378148..2380472(-) (recJ) [Bacillus sp. FSL K6-0994]
ATGTTATTATCCAAAATGCGCTGGGAATATGAACATCCAAGCGAAGAAAAAGTGAAATCACTCTCTGAAAATCTGAATAT
TTCAGCGCTGACTGCATCGCTTCTCGTAAAAAGAGGAATTGAAGAAGTAGAAGAGGCGAGGTCTTTTTTATTTGATCAAA
AAGCCGAATTTCATGATCCATTTCTATTAAAAGGAATGAAAGAAGCAGTAGAGCGCATCAACAAAGCGATCGAAGAGCAG
GAATCGATTGTGATCTTCGGAGACTATGATGCAGATGGTGTGACAAGTACGTCTGTACTTTTACATACATTAAAAGAGCG
GTCTGCGATAGTAGACTTTTATATTCCAGATCGATTTAAAGAAGGCTATGGGCCTAATGAACAGGCATTTCGATATATCA
AGGAGCAAGGAGCATCGCTTGTCATCACGGTCGATACAGGCATTGCTGCTGTAAATGAAGCACGTATTGCAAAAGAAATA
GGGTTAGACCTAATCATTACAGATCACCATGAACCAAGTGAGGAGCTGCCTGAGGCACTTGCTATTGTCCATCCTAAGCA
GCCTGGCTGTGAGTATCCCTTTAAAGAACTTGCTGGTGTAGGCGTAGCGTTTAAAGTGGCGCATGCACTGCTTGGGAAGC
TGCCAGTTCAGCTGTTAGATCTTGCAGCAATCGGGACAATCGCGGATCTTGTACCGCTTCATGATGAGAATAGATGGCTC
GCCAAAAAAGGCCTTGTGCAGCTTCGGCAGTCGAATAGACCGGGGCTGAAAGCGTTATTGAAAGAAGCGGGAGCCACTCT
AGAAGAAGCCAATGAGGAAACGGTCGGCTTTCAAATTGCGCCAAGGCTGAATGCTGTAGGACGAATTGAACAGGCAGATC
CTGCCGTTCATCTTCTGATGACTGAGGATAAGGATGAAGCAGAAGAGCTCGCTCGTTTTGTACAGGAACTGAATAAAGAA
CGTCAAAAGATTGTCAGTACGATCACAGAAGAAGCCATTCAAATGGTGGAAGAAACGCCTGATGACCAGTCTGCGATCGT
TGTAGCGAAAGCTGGCTGGAATCCGGGCGTTGTCGGAATTGTTGCCTCTAAATTGACGGATACTTTTTCTCGTCCGGCGA
TTGTGCTTGGAATAGATGAAGAGACGCAAATGGCAAAAGGATCAGCCCGGAGTATTCCTGGCTTCGATCTGTTTTTCCAT
CTAAGCAAATGCCGCGACATCCTGCCGCATTTCGGGGGGCACCCGATGGCAGCAGGGATGACACTTCGAGCGGATGATGT
CGCTTTACTAAGGGAAAGATTAAATCAATATGCAAATGAAACATTGACGGAAGAAGATTTTATTCCAATACAGCGGGTCG
ATGCCGTGTGCAAGGTAGACGAATTGACGGTTGAAGCGATTGAGGAAGCGGGGATGCTGTCACCATTCGGTATGTCAAAC
CCAAAACCGTTCATTATGATTGAAGATGCGAAGCTTGAAGATATCCGAACGATTGGGGCAAATCACAACCATATTAAAAT
GTCTCTGAAAGACGAAGACAAGCTGCTTGACTGTGTCGGTTTTCACCAAGGGAAATTAAAAGAAGAAATTGTTCCAGGAA
GCCGCATTTCCGTGATTGGAGAAATGTCCGTCAATGAATGGAATAACCGGAAGAAGCCCCAGCTGATGCTAAAAGATGCA
CGAGTTGATGAATGGCAGCTGTTTGATCTGCGCGGGAAAAAGGATTGGGAGAAGAAAGTCTCGACACTTGATTCAGAAAA
ATCTTTGGTTGTCTGTTTTGATGAAGCAACAAAACAGGACGTCGATATCCCCATTCATCATGTTCATGCAGAAAATGTAT
CAACGTTTGATGTACAGGGCAAATATGTGGTCTTTGCGGATGTTCCCGCAGATGAGCAATTGCTGAAGCAGCTTTTAGAA
AATCAAAAGCCTGCCAGAATTTATACGGTTTTTCAAAGAAAAGAAGATCATTTTATGTCCTCTTTCCCAAGCCGTGACCA
ATTCAAATGGTTTTATGGGTTCTTGTTTAAACGGGGAAGTTTTCCTATCAAAGAACAAGGAATGGACCTTGCCAAACATA
GAGGCTGGACAAAAGATACAATCATTTTTATGACAAAGGTGTTTTTTGAACTCGGTTTTGTTAAAATAGAGAATGGTGTG
CTGTCAATAGTACTTGATGCCCCTAAAAAAGATTTAACGGCATCAGCATCCTATACAGCAAAACAACGATTGATGGAACT
CGATCAAACACTAACATATTCCTCAGCTAAAGAGCTGAAGGAATGGCTGAATAGCATGATGAGCGAAGTCTCACCTGTGC
TTTGA

Domains

Predicted by InterproScan.

RecJ OB domain (RecJ_OB)

(453-559)

DHHA1 domain (DHHA1)

(309-437)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(566-763)

DHH family (DHH)

(83-226)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

67.31

100

0.676

Multiple sequence alignment