Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   MHI46_RS12505 Genome accession   NZ_CP152042
Coordinates   2417490..2419814 (-) Length   774 a.a.
NCBI ID   WP_342502836.1    Uniprot ID   -
Organism   Bacillus sp. FSL K6-1000     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2412490..2424814
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHI46_RS12490 (MHI46_12490) dtd 2414140..2414583 (-) 444 WP_059374662.1 D-aminoacyl-tRNA deacylase -
  MHI46_RS12495 (MHI46_12495) - 2414602..2416812 (-) 2211 WP_024427858.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
  MHI46_RS12500 (MHI46_12500) - 2416954..2417466 (-) 513 WP_024423133.1 adenine phosphoribosyltransferase -
  MHI46_RS12505 (MHI46_12505) recJ 2417490..2419814 (-) 2325 WP_342502836.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  MHI46_RS12510 (MHI46_12510) - 2419896..2420246 (-) 351 WP_039180309.1 lipopolysaccharide assembly protein LapA domain-containing protein -
  MHI46_RS12515 (MHI46_12515) - 2420348..2420836 (-) 489 WP_058838998.1 cation:proton antiporter regulatory subunit -
  MHI46_RS12520 (MHI46_12520) secDF 2420998..2423226 (-) 2229 WP_039180313.1 protein translocase subunit SecDF -
  MHI46_RS12525 (MHI46_12525) - 2423268..2423564 (-) 297 WP_003216788.1 post-transcriptional regulator -

Sequence

Protein


Download         Length: 774 a.a.        Molecular weight: 87055.64 Da        Isoelectric Point: 4.9181

>NTDB_id=986496 MHI46_RS12505 WP_342502836.1 2417490..2419814(-) (recJ) [Bacillus sp. FSL K6-1000]
MLLSKMRWEYEHPSEEKVKSLSENLNISALTASLLVKRGLEEVEEARSFLFDQKAEFHDPFLLKGMKEAVERINKAIEEQ
ESIVIFGDYDADGVTSTSVLLHTLKERSAIVDFYIPDRFKEGYGPNEQAFRYIKEQGASLVITVDTGIAAVNEARIAKEI
GLDLIITDHHEPSEELPEALAIVHPKQPGCEYPFKELAGVGVAFKVAHALLGELPVQLLDLAAIGTIADLVPLHDENRWL
AKKGLMQLRQSNRPGLKALLKEAGATLEEANEETVGFQIAPRLNAVGRIEQADPAVHLLMTDDKDEAEELARFVQELNKE
RQKIVSTITEEAIQMVEEAGDDQSAIVVAKAGWNPGVVGIVASKLTDTFSRPAIVLGIDEETQMAKGSARSIPGFDLFFH
LSKCRDILPHFGGHPMAAGMTLRADDVALLRERLNQYANETLTEEDFIPIQRVDAVCKVDELTVQAIEEAGMLSPFGMSN
PKPFIMIEDAKLEDIRTIGANHNHIKMSLKDEDKLLDCVGFHQGKLKEEIVPGSRISVIGEMSVNEWNNRKKPQLMLKDA
RVDEWQLFDLRGKKDWEKKVSTLDSEKSLIVCFEEATKQNVDIPIHHVHAENVSTFDVHGKYVVFADVPADEQLLKQLLE
NQKPARIYTVFQREEDHFMSSFPSRDQFKWFYGFLFKRGSFPIKEQGMELAKHRGWTKDTIIFMTKVFFELGFVKIENGV
LSIVLDAPKKDLTASASYTAKQRLMELDQTLTYSSAKELKEWLNSMMSEVSPVL

Nucleotide


Download         Length: 2325 bp        

>NTDB_id=986496 MHI46_RS12505 WP_342502836.1 2417490..2419814(-) (recJ) [Bacillus sp. FSL K6-1000]
ATGTTATTATCCAAAATGCGCTGGGAATATGAACATCCAAGCGAAGAAAAAGTGAAATCACTCTCTGAAAATCTGAATAT
TTCAGCGCTGACTGCATCGCTTCTCGTAAAAAGAGGACTTGAAGAAGTAGAAGAGGCGAGGTCTTTTTTATTTGATCAAA
AAGCCGAATTTCATGATCCATTCTTATTAAAAGGAATGAAAGAAGCAGTAGAGCGCATCAACAAAGCGATCGAAGAGCAG
GAATCGATTGTGATCTTCGGAGACTATGATGCAGATGGTGTGACAAGTACGTCTGTACTTTTACATACATTAAAAGAGCG
GTCTGCGATAGTAGACTTTTATATTCCAGATCGGTTTAAAGAAGGCTATGGGCCTAATGAACAGGCTTTTCGATATATCA
AGGAGCAAGGAGCATCGCTTGTCATCACGGTTGATACAGGCATTGCTGCTGTAAATGAAGCACGTATTGCAAAAGAAATA
GGGCTAGACCTGATCATTACAGATCACCATGAACCAAGTGAGGAGCTGCCTGAGGCACTTGCCATTGTCCATCCTAAACA
GCCAGGCTGTGAGTATCCCTTTAAAGAACTTGCTGGTGTAGGTGTAGCGTTTAAAGTGGCGCATGCACTTCTTGGGGAGT
TGCCAGTTCAGCTGTTAGATCTTGCAGCAATCGGGACAATCGCGGATCTTGTACCGCTTCATGATGAGAATAGATGGCTC
GCCAAAAAAGGGCTTATGCAGCTTCGTCAGTCGAATAGACCGGGGCTGAAAGCGTTATTGAAAGAAGCGGGAGCCACTTT
AGAAGAAGCGAACGAGGAAACGGTCGGCTTTCAAATTGCGCCAAGGCTGAATGCTGTAGGACGAATTGAACAGGCAGATC
CAGCTGTTCACCTTCTTATGACTGACGATAAGGATGAAGCAGAAGAGCTCGCTCGTTTTGTACAGGAACTGAATAAAGAA
CGACAAAAGATTGTCAGTACGATCACAGAAGAAGCCATTCAAATGGTGGAAGAGGCAGGCGATGACCAGTCTGCGATCGT
TGTAGCGAAAGCTGGCTGGAATCCGGGTGTTGTCGGAATTGTTGCCTCTAAATTGACGGATACTTTTTCTCGTCCGGCGA
TTGTGCTTGGAATAGATGAAGAAACGCAAATGGCAAAAGGATCAGCCCGGAGCATTCCTGGCTTCGATCTATTCTTCCAT
CTAAGCAAATGCCGTGACATCCTGCCGCATTTCGGCGGGCATCCGATGGCAGCAGGGATGACACTTCGAGCGGATGATGT
CGCTTTACTAAGGGAAAGATTAAATCAATATGCCAATGAAACATTGACGGAAGAAGATTTTATTCCCATTCAGCGGGTCG
ATGCCGTGTGCAAGGTAGACGAATTGACGGTTCAAGCGATTGAAGAAGCGGGGATGCTGTCACCATTTGGTATGTCAAAC
CCAAAACCGTTCATTATGATTGAAGATGCGAAGCTTGAAGATATCCGAACAATTGGGGCAAATCACAACCATATTAAAAT
GTCTCTGAAAGACGAAGACAAGCTGCTTGACTGTGTCGGCTTTCACCAAGGGAAATTAAAAGAAGAAATTGTTCCAGGAA
GCCGCATTTCCGTGATTGGAGAAATGTCCGTCAATGAATGGAATAACCGGAAAAAGCCCCAGCTAATGCTAAAAGATGCA
CGAGTTGATGAATGGCAGCTGTTTGATCTGCGCGGGAAAAAGGATTGGGAGAAGAAAGTTTCGACGCTTGATTCAGAAAA
ATCTTTGATTGTCTGTTTTGAGGAAGCAACAAAACAGAACGTCGATATCCCCATTCATCATGTTCATGCAGAAAATGTAT
CAACGTTTGATGTACACGGAAAGTATGTGGTCTTTGCGGATGTTCCCGCAGATGAGCAATTGCTGAAGCAGCTTTTAGAA
AATCAGAAGCCTGCCAGAATTTATACGGTTTTTCAAAGAGAAGAAGATCATTTTATGTCCTCTTTCCCAAGCCGTGATCA
ATTCAAATGGTTTTATGGGTTCTTGTTTAAACGGGGAAGTTTTCCTATCAAAGAACAAGGAATGGAGCTTGCCAAACATA
GAGGCTGGACAAAAGATACAATCATTTTTATGACAAAGGTGTTTTTTGAACTCGGTTTTGTTAAAATAGAGAATGGTGTG
CTGTCAATAGTACTTGATGCCCCTAAAAAAGATTTAACGGCATCAGCGTCCTATACAGCAAAACAACGATTGATGGAACT
CGATCAAACACTAACATATTCCTCAGCTAAAGAGCTGAAGGAATGGCTGAATAGCATGATGAGCGAAGTCTCACCTGTGC
TTTGA

Domains

Predicted by InterproScan.

RecJ OB domain (RecJ_OB)

(453-559)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(566-763)

DHHA1 domain (DHHA1)

(311-437)

DHH family (DHH)

(83-226)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

67.696

100

0.68

Multiple sequence alignment