Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   MHH31_RS05270 Genome accession   NZ_CP152041
Coordinates   1049026..1052535 (+) Length   1169 a.a.
NCBI ID   WP_342495347.1    Uniprot ID   -
Organism   Bacillus sp. FSL K6-1033     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1044026..1057535
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHH31_RS05240 (MHH31_05240) lepB 1044329..1044841 (+) 513 WP_007499139.1 signal peptidase I -
  MHH31_RS05245 (MHH31_05245) - 1045081..1046223 (-) 1143 WP_007499137.1 AbrB family transcriptional regulator -
  MHH31_RS05250 (MHH31_05250) - 1046499..1046921 (+) 423 WP_007499135.1 BlaI/MecI/CopY family transcriptional regulator -
  MHH31_RS05255 (MHH31_05255) - 1046914..1047753 (+) 840 WP_025207106.1 M56 family metallopeptidase -
  MHH31_RS05260 (MHH31_05260) - 1047811..1048221 (+) 411 WP_008348626.1 DoxX family protein -
  MHH31_RS05265 (MHH31_05265) - 1048413..1048859 (-) 447 WP_007499129.1 ferritin-like domain-containing protein -
  MHH31_RS05270 (MHH31_05270) addB 1049026..1052535 (+) 3510 WP_342495347.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  MHH31_RS05275 (MHH31_05275) addA 1052513..1056217 (+) 3705 WP_138278367.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  MHH31_RS05280 (MHH31_05280) sbcD 1056243..1057418 (+) 1176 WP_138278368.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1169 a.a.        Molecular weight: 134701.66 Da        Isoelectric Point: 5.2740

>NTDB_id=986408 MHH31_RS05270 WP_342495347.1 1049026..1052535(+) (addB) [Bacillus sp. FSL K6-1033]
MEIQFLAGRSGSGKTTAILEEIKEQLRLDPLGPPIIFLVPDQMTFLMEYELAKTSEAGGMIRTKVFSFTRLAWSILQQTG
GANRQFVTSTGIQMLLRKVIEEQKDKFKVFKKASDKPGFVEQIEKTMAEFKRYCMMPEDIEKISASSMHSEYTEERRAAE
KLHDLHVLYQQMEQHLQDEYVHSEDYLTLLAQQIPLSEELRGAHIYIDGFYQFTPQQLLVIEQLLLHAEKITAAFTVDRS
YHDTQPNELDLFRMTGKTYSQLYQLAKECGAAISENILERNKRHLHTPDLAYLENQYEQRPLQPYQEETPHLTVSKSANK
RAEIEGVARDILDLVRGEGLRLRDISIVARHVDEYKDTLKEVFRDYDIPFFIDGNESMQYHPLIEFIRSSLDVIKGNWRY
EAVFRCVKTEFLFPLELAKNKAREQADQLENYCIAYGVKGERWTNGSRFHYRRFQSLDEDFGQTDQEIEMEQMLNDVKEW
IVPPLFQLQKRLKKAQKLRDMVEAVYVFLEEAQVPEKLEKARLEAEEAGRLAEAMQHGQVWDAVIQLMDEFVEMLGEEEL
SFPLFQQMMDTGLSSLKFALIPPSLDQVFIGSMDLSRMYQVKCTFVIGVNDGVIPARPSDESVLSEDDREWLKRAGAELA
ETGKERLLDEQFLIYQALSSPSHYLYLSYSASDTEGRSLLPSTLIKYCEELMPNHQQALYVLDPEQLDDQEQLKFVTNEH
VSLSYTVSQLQQWLNQYPISGVWWSVYNHLMTSSNRDVSKKIMSSLFFTNRAKRLNPNVTKELYGDHIQGSVSRMEKFNA
CAFSHFASHGLKLKDRQFYKLEAPDIGQLFHSALKLISDTLVEQKKDWKNLTKEDCVTYSRHAIEQLAPRLQKEILLSSN
RHAYIKEKLQKILIRVSSILSEHAKVSGFSPVGLELGFGGQGPLPPFTFQLKNGCTMELVGRIDRVDKAEGSKGLLLRIV
DYKSSEKGLDLAEVYYGLALQMLTYLDLTITYSKEWLGMEATPAGILYFHIHDPLIQAPIPLGEDAIEQEILKKFKMKGL
LLEDVEAVKLMDQTLESGRSHVIQAGLKKDGSFRSDSAVLSEDHFHILTKHVRRTFEEAGERITSGEVEINPYKLKDQTP
CRFCSFKSICQFDESIEDNEFRVLTSEKDDVMLDRIKKEGDQYANTKTE

Nucleotide


Download         Length: 3510 bp        

>NTDB_id=986408 MHH31_RS05270 WP_342495347.1 1049026..1052535(+) (addB) [Bacillus sp. FSL K6-1033]
GTGGAGATTCAGTTTTTAGCAGGCAGATCGGGGAGTGGAAAAACGACTGCAATTCTAGAAGAAATCAAAGAACAACTGCG
GCTTGATCCCCTGGGTCCGCCAATCATTTTTTTAGTCCCGGATCAAATGACATTTTTAATGGAATATGAGCTTGCAAAAA
CGTCTGAAGCTGGTGGAATGATCAGGACAAAAGTCTTTAGTTTTACTCGGCTTGCCTGGTCTATATTACAGCAGACTGGC
GGTGCGAACCGTCAATTTGTCACAAGCACAGGGATTCAAATGCTCTTAAGAAAAGTAATAGAAGAACAGAAAGACAAGTT
TAAGGTATTCAAAAAGGCGAGTGATAAACCGGGGTTTGTTGAGCAAATAGAAAAGACGATGGCTGAATTCAAAAGGTACT
GTATGATGCCGGAGGATATTGAAAAGATCTCGGCATCCAGCATGCATTCAGAGTATACAGAAGAAAGACGTGCGGCGGAA
AAATTGCATGATCTTCATGTTCTTTATCAGCAGATGGAGCAGCATCTGCAAGATGAGTATGTGCACTCAGAGGATTACTT
GACACTTCTGGCTCAGCAAATTCCTTTATCAGAAGAATTGAGAGGGGCACACATTTATATAGATGGTTTTTATCAGTTTA
CCCCCCAGCAGCTTCTTGTCATTGAACAGCTTTTGCTACATGCCGAAAAAATAACAGCTGCATTCACAGTGGATCGTTCC
TATCATGACACGCAGCCAAATGAACTTGATTTATTTCGCATGACAGGTAAGACATATTCCCAGCTTTATCAGTTGGCCAA
AGAGTGCGGAGCGGCTATTTCTGAGAACATTTTGGAGAGAAATAAGCGGCATCTTCATACCCCAGATCTAGCTTATTTAG
AAAATCAATATGAACAGAGACCTTTGCAGCCGTATCAAGAAGAAACGCCTCACCTTACCGTATCGAAAAGTGCAAATAAA
CGAGCTGAAATTGAGGGAGTAGCGAGAGATATTCTTGATTTAGTAAGAGGTGAAGGTCTTAGGTTACGAGACATCTCAAT
TGTTGCAAGACATGTAGACGAATATAAAGATACGTTAAAAGAGGTGTTTCGAGATTACGATATTCCTTTCTTTATTGATG
GAAATGAATCGATGCAGTATCATCCGCTCATTGAGTTCATTCGTTCGAGCCTCGATGTCATAAAAGGGAACTGGCGGTAT
GAGGCGGTGTTTCGCTGTGTGAAAACGGAGTTCTTATTTCCGCTTGAACTCGCCAAAAACAAAGCGAGAGAACAGGCGGA
TCAGCTTGAAAACTATTGTATTGCTTACGGTGTAAAAGGTGAACGCTGGACGAATGGTTCACGCTTTCATTATCGCCGCT
TCCAATCACTAGATGAAGATTTTGGACAAACCGATCAAGAAATAGAAATGGAGCAAATGCTGAATGATGTAAAAGAATGG
ATTGTTCCGCCTCTCTTTCAATTGCAAAAAAGATTGAAAAAAGCGCAAAAGCTAAGAGACATGGTGGAGGCAGTGTATGT
CTTTCTAGAAGAAGCCCAAGTGCCAGAAAAGCTTGAAAAGGCAAGGCTTGAGGCAGAAGAAGCAGGCAGGTTAGCTGAAG
CGATGCAGCATGGGCAAGTGTGGGATGCTGTCATTCAATTGATGGATGAATTTGTTGAAATGCTGGGCGAGGAGGAGCTC
TCATTTCCATTATTTCAACAGATGATGGATACAGGACTGTCCTCTCTCAAGTTTGCTTTGATTCCCCCTTCACTTGATCA
AGTGTTTATCGGAAGTATGGATTTATCCAGAATGTATCAAGTAAAGTGTACGTTTGTCATTGGAGTAAATGATGGGGTTA
TACCTGCACGGCCCTCTGATGAAAGTGTATTGTCTGAAGACGACCGGGAATGGTTAAAGCGTGCAGGAGCAGAGCTGGCA
GAGACAGGGAAGGAACGGCTGCTGGATGAACAATTTTTGATATATCAAGCGCTGTCCAGTCCATCGCACTACCTGTATTT
ATCCTATTCAGCTTCAGATACGGAAGGGCGGTCTCTTTTGCCTTCGACCCTCATCAAGTATTGTGAGGAACTGATGCCAA
ACCATCAGCAAGCCCTCTATGTGTTAGATCCAGAGCAGTTGGATGATCAGGAGCAATTGAAATTTGTAACAAACGAGCAT
GTCTCGTTATCCTATACCGTTTCGCAGCTACAACAATGGCTCAATCAATATCCGATCAGCGGCGTATGGTGGAGTGTATA
TAATCATTTGATGACCTCGTCCAACCGCGACGTATCAAAAAAAATCATGTCCAGCTTGTTCTTTACAAATAGAGCGAAAC
GTTTAAATCCGAATGTCACAAAAGAACTTTACGGTGACCATATTCAGGGCAGTGTTTCAAGAATGGAGAAATTCAATGCA
TGTGCATTTTCTCATTTTGCTTCCCACGGGCTAAAACTAAAAGATCGACAATTTTACAAATTAGAAGCACCTGATATCGG
CCAGCTCTTTCACTCGGCTTTAAAACTGATTTCAGATACGCTTGTTGAACAAAAAAAGGATTGGAAAAACTTAACGAAGG
AAGATTGTGTGACCTACTCAAGGCATGCAATTGAACAGCTGGCTCCACGTCTGCAAAAAGAAATTTTATTAAGCTCTAAT
CGGCATGCTTATATAAAAGAAAAGCTTCAAAAAATCCTCATCAGGGTGTCTTCTATATTAAGCGAACATGCAAAAGTGAG
CGGATTCTCTCCGGTTGGACTTGAGTTAGGTTTTGGAGGTCAAGGACCTCTGCCGCCGTTTACTTTTCAATTAAAAAACG
GCTGCACGATGGAGCTTGTTGGAAGGATTGACCGAGTGGATAAAGCAGAAGGATCAAAAGGACTTCTATTAAGAATTGTC
GACTATAAGTCCAGTGAGAAGGGGCTTGATCTAGCAGAAGTTTACTACGGTTTAGCTTTGCAAATGCTCACTTATTTAGA
CCTGACCATTACGTATTCTAAAGAATGGCTTGGCATGGAGGCGACACCGGCAGGAATTTTATACTTCCATATTCATGACC
CGCTGATTCAAGCCCCTATTCCGCTTGGAGAAGATGCAATTGAGCAGGAAATATTGAAGAAATTTAAAATGAAGGGTCTA
TTGCTCGAAGATGTGGAAGCAGTGAAGTTGATGGATCAAACACTCGAATCAGGAAGATCACATGTCATCCAAGCTGGTTT
GAAAAAAGATGGTTCTTTCCGATCTGACTCTGCGGTATTAAGTGAAGATCATTTTCACATTCTCACAAAACACGTTCGGC
GAACCTTTGAAGAGGCAGGAGAAAGAATTACAAGCGGTGAGGTAGAGATAAACCCATATAAATTAAAGGATCAAACCCCT
TGCCGCTTTTGCTCCTTTAAGTCTATTTGCCAATTTGATGAATCAATAGAAGATAATGAATTTAGGGTGCTCACCTCTGA
GAAGGATGATGTCATGTTAGACCGGATCAAAAAAGAAGGGGATCAGTATGCAAATACCAAAACCGAATAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

66.494

99.316

0.66

Multiple sequence alignment