Detailed information    

insolico Bioinformatically predicted

Overview

Name   endA   Type   Machinery gene
Locus tag   R8623_RS09250 Genome accession   NZ_AP026928
Coordinates   1798801..1799625 (-) Length   274 a.a.
NCBI ID   WP_001036784.1    Uniprot ID   A0A0E7E4I0
Organism   Streptococcus pneumoniae strain PZ900700406     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1793801..1804625
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R8623_RS09245 (PC0401_17940) - 1797179..1798510 (-) 1332 WP_016398203.1 hemolysin family protein -
  R8623_RS09250 (PC0401_17950) endA 1798801..1799625 (-) 825 WP_001036784.1 DNA-entry nuclease EndA Machinery gene
  R8623_RS09255 (PC0401_17960) - 1799664..1799852 (-) 189 WP_001813390.1 DNA-directed RNA polymerase subunit beta -
  R8623_RS09260 (PC0401_17970) murA 1799845..1801128 (-) 1284 WP_000358028.1 UDP-N-acetylglucosamine 1-carboxyvinyltransferase -
  R8623_RS09265 (PC0401_17980) - 1801152..1801382 (-) 231 WP_000250393.1 DUF1146 family protein -
  R8623_RS09270 (PC0401_17990) sepM 1801440..1802477 (-) 1038 WP_000727277.1 SepM family pheromone-processing serine protease Regulator
  R8623_RS09275 (PC0401_18000) coaD 1802461..1802949 (-) 489 WP_001280742.1 pantetheine-phosphate adenylyltransferase -
  R8623_RS09280 (PC0401_18010) rsmD 1802939..1803478 (-) 540 WP_000706962.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  R8623_RS09285 (PC0401_18020) asnA 1803543..1804535 (-) 993 WP_000747993.1 aspartate--ammonia ligase -

Sequence

Protein


Download         Length: 274 a.a.        Molecular weight: 29931.63 Da        Isoelectric Point: 10.1197

>NTDB_id=98611 R8623_RS09250 WP_001036784.1 1798801..1799625(-) (endA) [Streptococcus pneumoniae strain PZ900700406]
MNKKTRQTLIGLLVLLLLSTGSYYIKQMQSAPNSPKTNLSQKKQAPEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIV
NGNKTNLDAKVSSKPYADNKTKTVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRVRYRVTLYYASNEDLVPSASQIE
AKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ

Nucleotide


Download         Length: 825 bp        

>NTDB_id=98611 R8623_RS09250 WP_001036784.1 1798801..1799625(-) (endA) [Streptococcus pneumoniae strain PZ900700406]
ATGAACAAAAAAACAAGACAGACACTAATCGGACTGCTAGTGTTATTGCTTTTGTCTACAGGGAGCTATTATATCAAGCA
GATGCAGTCGGCACCTAATAGTCCCAAAACCAATCTTAGTCAGAAAAAACAAGCGCCTGAAGCTCCTAGTCAAGCATTGG
CAGAGAGTGTCTTAACAGACGCAGTCAAGAGTCAAATAAAGGGGAGTCTGGAGTGGAATGGCTCAGGTGCTTTTATCGTC
AATGGTAATAAAACAAATCTAGATGCCAAGGTTTCAAGTAAGCCCTACGCTGACAATAAAACAAAGACAGTGGGCAAGGA
AACTGTTCCAACCGTAGCTAATGCCCTCTTGTCTAAGGCCACTCGTCAGTACAAGAATCGTAAAGAAACTGGGAATGGTT
CAACTTCTTGGACTCCTCCAGGTTGGCATCAGGTCAAGAATCTAAAGGGCTCTTATACCCATGCAGTCGATAGAGGTCAT
TTGTTAGGCTATGCCTTAATCGGTGGTTTGGATGGTTTTGATGCCTCAACAAGCAATCCTAAAAACATTGCTGTTCAGAC
AGCCTGGGCAAATCAGGCACAAGCCGAGTATTCGACTGGTCAAAACTACTATGAAAGCAAGGTGCGTAAAGCCTTGGACC
AAAACAAGCGTGTTCGTTACCGTGTAACCCTTTACTACGCTTCAAACGAGGATTTAGTTCCCTCAGCTTCACAGATTGAA
GCCAAGTCTTCGGATGGAGAATTGGAATTCAATGTTCTAGTTCCCAATGTTCAAAAGGGACTTCAACTGGATTACCGAAC
TGGAGAAGTAACTGTAACTCAGTAA

Domains

Predicted by InterproScan.

DNA/RNA non-specific endonuclease (Endonuclease_NS)

(75-247)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0E7E4I0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  endA Streptococcus pneumoniae Rx1

99.27

100

0.993
  endA Streptococcus pneumoniae D39

99.27

100

0.993
  endA Streptococcus pneumoniae R6

99.27

100

0.993
  endA Streptococcus pneumoniae TIGR4

99.27

100

0.993

Multiple sequence alignment