Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   MHI58_RS13070 Genome accession   NZ_CP152035
Coordinates   2520490..2522823 (-) Length   777 a.a.
NCBI ID   WP_035390967.1    Uniprot ID   -
Organism   Bacillus sp. FSL K6-2869     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2515490..2527823
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHI58_RS13055 (MHI58_13055) dtd 2517139..2517582 (-) 444 WP_024718380.1 D-aminoacyl-tRNA deacylase -
  MHI58_RS13060 (MHI58_13060) - 2517601..2519811 (-) 2211 WP_008342726.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
  MHI58_RS13065 (MHI58_13065) - 2519954..2520466 (-) 513 WP_008342729.1 adenine phosphoribosyltransferase -
  MHI58_RS13070 (MHI58_13070) recJ 2520490..2522823 (-) 2334 WP_035390967.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  MHI58_RS13075 (MHI58_13075) - 2522905..2523261 (-) 357 WP_024718382.1 lipopolysaccharide assembly LapA domain-containing protein -
  MHI58_RS13080 (MHI58_13080) - 2523361..2523849 (-) 489 WP_008342734.1 cation:proton antiporter regulatory subunit -
  MHI58_RS13085 (MHI58_13085) secDF 2524050..2526278 (-) 2229 WP_045034111.1 protein translocase subunit SecDF -
  MHI58_RS13090 (MHI58_13090) - 2526320..2526616 (-) 297 WP_003216788.1 post-transcriptional regulator -

Sequence

Protein


Download         Length: 777 a.a.        Molecular weight: 87616.39 Da        Isoelectric Point: 4.8057

>NTDB_id=986076 MHI58_RS13070 WP_035390967.1 2520490..2522823(-) (recJ) [Bacillus sp. FSL K6-2869]
MLLSKMRWEYEHPSEEKVKSLSENLNISALTASLLVQRGLEEVEEARSFLFDQKAEFHDPFLLKGMKEAVERINKAIEEQ
ESIVIFGDYDADGVTSTSVLLHTLKERSAKVDFYIPDRFKEGYGPNEQAFRYIKEQGASLVITVDTGIAAVNEARIAKEI
GLDLIITDHHEPSEELPEALAIVHPKQPGCEYPFKELAGVGVAFKVAHALLGKLPVELLDLAAIGTIADLVPLHDENRWI
AKKGLMQLRQSRRPGLKALLKEAGATLEEANEETVGFQIAPRLNAVGRIEQADPAVHLLMTEDMDEAEELARFVQELNKE
RQKIVSTITEEAIQMVEETGADQSAIVVAKAGWNPGVVGIVASKLTDTFSRPAIVLGIDEETQMAKGSARSIPGFDLFFH
LSKCRDILPHFGGHPMAAGMTLQADDVPLLRERLNQYANETLTEEDFIPIQRVDAVCKVDEMTVQAIEEVGLLSPFGMQN
PKPFIMIEDAQLEDIRTIGANQNHIKMSLKDEDKLLDCVGFHQGKLKEELVPGSQISVVGEVSINEWNNRKKPQLMLKDA
RVDEWQLFDLRGKKDWEKKVSTLDPAKTMVFCFDEETKKQSELVDIPIHLIDAENVSTFDVQEKYVIFADVPADEHLLKL
LLENQQPARIYTVFQRKEDHFMSSFPSRDQFKWFYGFLFKRGSFPIKEQGMELAKHRGWTKDTIIFMTKVFFELGFVKIE
NGVLSIVRDAPKKDLTASSSYTAKQRLMELDQTLTYSSAKELKEWLNSMMSEVSPVL

Nucleotide


Download         Length: 2334 bp        

>NTDB_id=986076 MHI58_RS13070 WP_035390967.1 2520490..2522823(-) (recJ) [Bacillus sp. FSL K6-2869]
ATGTTATTATCCAAAATGCGTTGGGAATATGAACATCCTAGCGAAGAAAAAGTGAAATCACTCTCTGAAAATCTGAATAT
TTCAGCGCTGACTGCATCACTTCTTGTACAAAGAGGGCTCGAAGAAGTAGAAGAAGCGAGGTCTTTTTTATTTGATCAAA
AAGCCGAATTTCATGATCCATTCTTATTAAAAGGAATGAAAGAAGCAGTAGAGCGCATCAACAAAGCGATCGAAGAGCAG
GAATCGATTGTGATCTTCGGAGATTATGATGCAGATGGTGTGACAAGTACGTCTGTACTTTTACATACACTTAAAGAACG
ATCTGCGAAGGTAGACTTTTATATTCCAGATCGATTTAAAGAAGGATATGGGCCAAACGAACAGGCTTTCCGATATATCA
AAGAGCAAGGAGCATCGCTTGTCATCACTGTGGATACAGGGATCGCTGCTGTAAATGAAGCACGTATTGCAAAAGAAATA
GGATTAGACCTGATCATCACAGATCACCATGAACCAAGCGAGGAATTGCCTGAGGCACTTGCGATTGTACATCCTAAGCA
GCCTGGGTGTGAGTATCCGTTTAAAGAGCTGGCGGGTGTCGGTGTGGCGTTTAAAGTGGCACATGCACTCCTCGGTAAAC
TCCCAGTAGAATTATTAGATCTTGCTGCAATCGGTACAATTGCGGATCTTGTCCCGCTGCATGATGAAAATAGATGGATT
GCCAAAAAAGGCCTAATGCAGCTTCGACAGTCTAGACGGCCTGGGTTAAAAGCGTTATTAAAAGAAGCAGGCGCCACTCT
TGAAGAAGCAAATGAGGAAACGGTCGGCTTTCAAATTGCGCCAAGGCTGAATGCTGTTGGACGAATTGAGCAGGCAGATC
CGGCTGTTCATCTTCTCATGACTGAGGATATGGATGAAGCAGAAGAACTCGCTCGTTTTGTACAAGAATTGAACAAAGAA
CGGCAGAAAATTGTGAGTACGATCACGGAAGAAGCCATTCAAATGGTCGAAGAGACAGGTGCTGACCAGTCTGCTATCGT
CGTAGCAAAAGCTGGCTGGAATCCAGGCGTTGTCGGCATTGTTGCCTCAAAATTGACGGATACATTTTCTCGTCCGGCGA
TTGTGCTTGGAATAGATGAAGAAACGCAGATGGCGAAAGGGTCAGCACGAAGTATTCCTGGCTTTGATTTATTTTTCCAT
TTGAGCAAGTGCCGTGACATCCTGCCGCATTTCGGTGGACACCCGATGGCTGCTGGAATGACACTTCAAGCGGATGATGT
TCCTTTACTTAGGGAAAGATTGAATCAATATGCAAATGAAACGTTGACAGAAGAAGATTTCATTCCAATTCAACGGGTCG
ATGCAGTATGTAAAGTGGACGAAATGACGGTGCAAGCCATTGAAGAAGTGGGGCTGCTGTCCCCGTTTGGCATGCAGAAT
CCTAAGCCGTTCATTATGATTGAAGACGCCCAGCTTGAAGACATCCGAACGATCGGGGCCAATCAAAATCACATTAAAAT
GTCACTAAAAGACGAAGACAAGCTGCTCGACTGTGTCGGTTTTCACCAAGGCAAACTAAAAGAAGAACTTGTCCCAGGGA
GCCAAATTTCTGTCGTTGGCGAGGTCTCGATTAATGAATGGAATAACCGAAAGAAACCTCAGCTCATGCTAAAAGACGCT
AGAGTCGATGAATGGCAGCTGTTTGATTTACGCGGGAAAAAAGATTGGGAAAAGAAAGTATCAACACTTGATCCAGCAAA
AACGATGGTCTTCTGTTTTGACGAAGAAACAAAAAAGCAATCGGAGCTGGTGGATATTCCGATTCATCTTATTGATGCAG
AAAATGTATCCACGTTTGATGTGCAGGAAAAGTATGTGATCTTTGCCGATGTTCCTGCCGATGAACACTTGCTCAAGCTG
CTTTTAGAAAATCAACAACCAGCAAGAATTTATACGGTGTTTCAGCGGAAAGAAGATCACTTTATGTCCTCATTCCCGAG
CCGTGATCAATTCAAATGGTTTTATGGGTTCTTGTTCAAACGGGGAAGTTTTCCTATTAAAGAACAAGGCATGGAGCTTG
CCAAACATAGAGGTTGGACAAAAGATACAATCATTTTTATGACAAAGGTGTTTTTTGAACTCGGTTTTGTTAAAATAGAG
AATGGTGTGCTGTCAATAGTGCGTGATGCCCCTAAAAAAGATTTAACGGCATCATCGTCTTATACAGCAAAACAACGATT
GATGGAACTCGATCAAACACTAACATATTCCTCAGCCAAAGAGCTGAAGGAATGGCTGAATAGCATGATGAGCGAAGTCT
CACCTGTGCTTTGA

Domains

Predicted by InterproScan.

DHH family (DHH)

(83-226)

RecJ OB domain (RecJ_OB)

(453-559)

DHHA1 domain (DHHA1)

(314-437)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(566-766)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

67.181

100

0.672

Multiple sequence alignment