Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   MHB66_RS12635 Genome accession   NZ_CP152027
Coordinates   2440122..2442446 (-) Length   774 a.a.
NCBI ID   WP_046311915.1    Uniprot ID   -
Organism   Bacillus sp. FSL L8-0167     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2435122..2447446
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHB66_RS12620 (MHB66_12620) dtd 2436772..2437215 (-) 444 WP_034279904.1 D-aminoacyl-tRNA deacylase -
  MHB66_RS12625 (MHB66_12625) - 2437234..2439444 (-) 2211 WP_034279902.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
  MHB66_RS12630 (MHB66_12630) - 2439586..2440098 (-) 513 WP_034279900.1 adenine phosphoribosyltransferase -
  MHB66_RS12635 (MHB66_12635) recJ 2440122..2442446 (-) 2325 WP_046311915.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  MHB66_RS12640 (MHB66_12640) - 2442528..2442878 (-) 351 WP_034279896.1 lipopolysaccharide assembly protein LapA domain-containing protein -
  MHB66_RS12645 (MHB66_12645) - 2442980..2443468 (-) 489 WP_041115109.1 cation:proton antiporter regulatory subunit -
  MHB66_RS12650 (MHB66_12650) secDF 2443655..2445883 (-) 2229 WP_034279892.1 protein translocase subunit SecDF -
  MHB66_RS12655 (MHB66_12655) - 2445925..2446221 (-) 297 WP_003216788.1 post-transcriptional regulator -

Sequence

Protein


Download         Length: 774 a.a.        Molecular weight: 87016.63 Da        Isoelectric Point: 4.9495

>NTDB_id=985643 MHB66_RS12635 WP_046311915.1 2440122..2442446(-) (recJ) [Bacillus sp. FSL L8-0167]
MLLSKMRWEYEHPSEEKVKSLSENLNISALTASLLVKRGIEEVEEARSFLFDQKAEFHDPFLLKGMKEAVERINKAIEEQ
ESIVIFGDYDADGVTSTSVLLHTLKERSAIVDFYIPDRFKEGYGPNEQAFRYIKEQGASLVITVDTGIAAVNEARIAKEI
GLDLIITDHHEPSEELPEALAIVHPKQPGCEYPFKELAGVGVAFKVAHALLGKLPVQLLDLAAIGTIADLVPLHDENRWL
AKKGLVQLRQSNRPGLKALLKEAGATLEEANEETVGFQIAPRLNAVGRIEQADPAVHLLMTEDKDEAEELARFVQELNKE
RQKIVSTITEEAIQMVEETGDDQSAIVVAKAGWNPGVVGIVASKLTDTFSRPAIVLGIDEETQMAKGSARSIPGFDLFFH
LSKCRDILPHFGGHPMAAGMTLRADDVALLRERLNQYANETLTEEDFIPIQRVDAVCKVDELTVEAIEEAGMLSPFGMSN
PKPFIMIEDAKLEDIRTIGANHNHIKMSLKDEDKLLDCVGFHQGKLKEEIVPGSRISVIGEMSVNEWNNRKKPQLMLKDA
RVDEWQLFDLRGKKDWEKKVSTLDSEKSLVVCFDEATKQDVDIPIHHVHAENVSTFDVQGKYVVFADVPADEQLLKQLLE
NQKPARIYTVFQRKEDHFMSSFPSRDQFKWFYGFLFKRGSFPIKEQGMDLAKHRGWTKDTIIFMTKVFFELGFVKIENGV
LSIVLDAPKKDLTASASYTAKQRLMELDQTLTYSSAKELKEWLNSMMSEVSPVL

Nucleotide


Download         Length: 2325 bp        

>NTDB_id=985643 MHB66_RS12635 WP_046311915.1 2440122..2442446(-) (recJ) [Bacillus sp. FSL L8-0167]
ATGTTATTATCCAAAATGCGCTGGGAATATGAACATCCAAGCGAAGAAAAAGTGAAATCACTCTCTGAAAATCTGAATAT
TTCAGCGCTGACTGCATCGCTTCTCGTAAAAAGAGGAATTGAAGAAGTAGAAGAGGCGAGGTCTTTTTTATTTGATCAAA
AAGCCGAATTTCATGATCCATTTCTATTAAAAGGAATGAAAGAAGCAGTAGAGCGCATCAACAAAGCGATCGAAGAGCAG
GAATCGATTGTGATCTTCGGAGACTATGATGCAGATGGTGTGACAAGTACGTCTGTACTTTTACATACATTAAAAGAGCG
GTCTGCGATAGTAGACTTTTATATTCCAGATCGATTTAAAGAAGGCTATGGGCCTAATGAACAGGCATTTCGATATATCA
AGGAGCAAGGAGCATCGCTTGTCATCACGGTCGATACAGGCATTGCTGCTGTAAATGAAGCACGTATTGCAAAAGAAATA
GGGTTAGACCTGATCATTACAGATCACCATGAACCAAGTGAGGAGCTGCCTGAGGCACTTGCCATTGTCCATCCTAAGCA
GCCTGGCTGTGAGTATCCCTTTAAAGAACTTGCTGGTGTAGGCGTAGCGTTTAAAGTGGCGCATGCACTGCTTGGGAAGC
TGCCAGTACAGCTGTTAGATCTTGCAGCAATCGGGACAATCGCGGATCTTGTACCGCTTCATGATGAGAATAGGTGGCTC
GCCAAAAAAGGCCTTGTACAGCTTCGGCAGTCGAATAGACCGGGGCTGAAAGCGTTATTGAAAGAAGCGGGAGCCACTCT
AGAAGAAGCCAATGAGGAAACGGTCGGCTTTCAAATTGCGCCAAGGCTGAATGCTGTAGGACGAATTGAACAGGCAGATC
CTGCCGTTCATCTTCTGATGACTGAGGATAAGGATGAAGCAGAAGAGCTCGCTCGTTTTGTACAGGAACTGAATAAAGAA
CGTCAAAAGATTGTCAGTACGATCACAGAAGAAGCCATTCAAATGGTGGAAGAGACAGGTGATGACCAGTCTGCGATCGT
TGTAGCGAAAGCTGGCTGGAATCCGGGCGTTGTCGGAATTGTTGCCTCTAAATTGACGGATACTTTTTCTCGTCCGGCGA
TTGTGCTTGGAATAGATGAAGAGACGCAAATGGCAAAAGGATCAGCCCGGAGTATTCCTGGCTTCGATCTGTTTTTCCAT
CTAAGCAAATGCCGCGACATCCTGCCGCATTTCGGGGGGCACCCGATGGCAGCAGGGATGACACTTCGAGCGGATGATGT
CGCTTTACTAAGGGAAAGATTAAATCAATATGCAAATGAAACATTGACGGAAGAAGATTTTATTCCAATACAGCGGGTCG
ATGCAGTATGCAAGGTAGACGAATTGACGGTTGAAGCGATTGAGGAAGCGGGGATGCTGTCACCATTCGGTATGTCAAAC
CCAAAACCGTTCATTATGATTGAAGATGCGAAGCTTGAAGATATCCGAACGATTGGGGCAAATCACAACCATATTAAAAT
GTCTCTGAAAGACGAAGACAAGCTGCTTGACTGTGTCGGTTTTCACCAAGGGAAATTAAAAGAAGAAATTGTTCCAGGAA
GCCGCATTTCCGTGATTGGAGAAATGTCCGTCAATGAATGGAATAACCGGAAGAAGCCCCAGCTGATGCTAAAAGATGCA
CGAGTTGATGAATGGCAGCTGTTTGATCTGCGCGGGAAAAAGGATTGGGAGAAGAAAGTTTCGACGCTTGATTCAGAAAA
ATCTTTGGTTGTCTGTTTTGATGAAGCAACAAAACAGGACGTCGATATCCCCATTCATCATGTTCATGCAGAAAATGTAT
CAACGTTTGATGTACAGGGCAAATATGTGGTCTTTGCGGATGTTCCCGCAGATGAGCAATTGCTGAAGCAGCTTTTAGAA
AATCAAAAGCCTGCCAGAATTTATACGGTTTTTCAAAGAAAAGAAGATCATTTTATGTCCTCTTTCCCAAGCCGTGACCA
ATTCAAATGGTTTTATGGGTTCTTGTTTAAACGGGGAAGTTTTCCTATCAAAGAACAAGGAATGGACCTTGCCAAACATA
GAGGCTGGACAAAAGATACAATCATTTTTATGACAAAGGTGTTTTTTGAACTCGGTTTTGTTAAAATAGAGAATGGTGTG
CTGTCAATAGTACTTGATGCCCCTAAAAAAGATTTAACGGCATCAGCATCCTATACAGCAAAACAACGATTGATGGAACT
CGATCAAACACTAACATATTCCTCAGCTAAAGAGCTGAAGGAATGGCTGAATAGCATGATGAGCGAAGTCTCACCTGTGC
TTTGA

Domains

Predicted by InterproScan.

DHHA1 domain (DHHA1)

(309-437)

RecJ OB domain (RecJ_OB)

(453-559)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(566-763)

DHH family (DHH)

(83-226)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

67.439

100

0.677

Multiple sequence alignment