Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   MHH65_RS12645 Genome accession   NZ_CP152026
Coordinates   2448730..2451054 (-) Length   774 a.a.
NCBI ID   WP_268414551.1    Uniprot ID   -
Organism   Bacillus sp. FSL L8-0654     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2443730..2456054
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHH65_RS12630 (MHH65_12630) dtd 2445380..2445823 (-) 444 WP_098676994.1 D-aminoacyl-tRNA deacylase -
  MHH65_RS12635 (MHH65_12635) - 2445842..2448052 (-) 2211 WP_034279902.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
  MHH65_RS12640 (MHH65_12640) - 2448194..2448706 (-) 513 WP_267587217.1 adenine phosphoribosyltransferase -
  MHH65_RS12645 (MHH65_12645) recJ 2448730..2451054 (-) 2325 WP_268414551.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  MHH65_RS12650 (MHH65_12650) - 2451136..2451486 (-) 351 WP_024423135.1 lipopolysaccharide assembly protein LapA domain-containing protein -
  MHH65_RS12655 (MHH65_12655) - 2451588..2452076 (-) 489 WP_034279894.1 cation:proton antiporter regulatory subunit -
  MHH65_RS12660 (MHH65_12660) secDF 2452263..2454491 (-) 2229 WP_268414552.1 protein translocase subunit SecDF -
  MHH65_RS12665 (MHH65_12665) - 2454533..2454829 (-) 297 WP_003216788.1 post-transcriptional regulator -

Sequence

Protein


Download         Length: 774 a.a.        Molecular weight: 86988.58 Da        Isoelectric Point: 4.9468

>NTDB_id=985582 MHH65_RS12645 WP_268414551.1 2448730..2451054(-) (recJ) [Bacillus sp. FSL L8-0654]
MLLSKMRWEYEHPSEEKVKSLSENLNISALTASLLVKRGLEEVEEARSFLFDQKAEFHDPFLLKGMKEAVERINKAIEEQ
ESIVIFGDYDADGVTSTSVLLHTLKERSAIVDFYIPDRFKEGYGPNEQAFRYIKEQGASLVITVDTGIAAVNEARIAKEI
GLDLIITDHHEPSEELPEALAIVHPKQPGCEYPFKELAGVGVAFKVAHALLGKLPVQLLDLAAIGTIADLVPLHDENRWL
AKKGLVQLRQSNRPGLKALLKEAGATLEEANEETVGFQIAPRLNAVGRIEQADPAVHLLMTEDKDEAEELARFVQELNKE
RQKIVSTITEEAIQMVEEAGDDQSAIVVAKAGWNPGVVGIVASKLTDTFSRPAIVLGIDEETQMAKGSARSIPGFDLFFH
LSKCRDILPHFGGHPMAAGMTLRADDVSLLRERLNQYANETLTEEDFIPIQRVDAVCKVDELTVQAIEEAGMLSPFGMSN
PKPFIMIEDAKLEDIRTIGANHNHIKMSLKDEDTLLDCVGFHQGKLKEEIVPGSRISVIGEMSVNEWNNRKKPQLMLKDA
RVDEWQLFDLRGKKDWEKKVSTLDSEKSLVVCFDEATKQDVDLPIHHVHAENVSTFDVQGKYVVFADVPADEQLLKQLLE
NQKPARIYTVFQRKEDHFMSSFPSRDQFKWFYGFLFKRGSFPIKEQGMELAKHRGWTKDTIIFMTKVFFELGFVKIENGV
LSIVLDAPKKDLTASASYTAKQRLMELDQTLTYSSAKELKEWLNSMMSEVSPVL

Nucleotide


Download         Length: 2325 bp        

>NTDB_id=985582 MHH65_RS12645 WP_268414551.1 2448730..2451054(-) (recJ) [Bacillus sp. FSL L8-0654]
ATGTTATTATCCAAAATGCGCTGGGAATATGAACATCCAAGCGAAGAAAAAGTGAAATCACTCTCTGAAAATCTGAATAT
TTCAGCGCTGACTGCATCGCTTCTCGTAAAAAGAGGACTTGAAGAAGTAGAAGAGGCGAGGTCTTTTTTATTTGATCAAA
AAGCCGAATTTCATGATCCATTTCTATTAAAAGGAATGAAAGAAGCAGTAGAGCGCATCAACAAAGCGATCGAAGAGCAG
GAATCGATTGTGATCTTCGGAGACTATGATGCAGATGGTGTGACAAGTACGTCTGTACTTTTACATACATTAAAAGAGCG
GTCTGCGATAGTAGACTTTTATATTCCAGATCGATTTAAAGAAGGCTATGGGCCTAATGAACAGGCATTTCGATATATCA
AGGAGCAAGGAGCATCGCTTGTCATCACGGTCGATACAGGCATTGCTGCTGTAAATGAAGCACGTATTGCAAAAGAAATA
GGGTTAGACCTGATCATTACAGATCACCATGAACCAAGTGAGGAGCTGCCTGAGGCACTTGCCATTGTCCATCCTAAGCA
GCCTGGCTGTGAGTATCCCTTTAAAGAACTTGCTGGTGTAGGCGTAGCGTTTAAAGTGGCGCATGCACTGCTTGGGAAGC
TGCCAGTACAGCTGTTAGATCTTGCAGCAATCGGGACAATCGCGGATCTTGTACCGCTTCATGATGAGAATAGGTGGCTC
GCCAAAAAAGGCCTTGTACAGCTTCGGCAGTCGAATAGACCGGGGCTGAAAGCGTTATTGAAAGAAGCGGGAGCCACTCT
AGAAGAAGCCAATGAGGAAACGGTCGGCTTTCAAATTGCGCCAAGGCTGAATGCTGTTGGGCGAATTGAACAGGCAGATC
CAGCCGTTCATCTTCTGATGACTGAGGATAAGGATGAAGCAGAAGAGCTCGCTCGTTTTGTACAGGAACTGAATAAAGAA
CGACAAAAGATTGTCAGTACGATCACAGAAGAAGCCATTCAAATGGTGGAAGAGGCAGGTGATGACCAGTCTGCGATTGT
TGTAGCGAAAGCTGGCTGGAATCCGGGCGTTGTCGGAATTGTTGCCTCTAAATTGACGGATACTTTTTCTCGTCCGGCGA
TTGTGCTTGGAATAGATGAAGAGACGCAAATGGCAAAAGGATCAGCCCGGAGTATTCCTGGTTTCGATCTATTTTTCCAT
CTAAGCAAATGCCGCGACATCCTGCCGCATTTCGGCGGGCATCCGATGGCAGCAGGGATGACACTTCGAGCGGATGATGT
ATCTTTACTAAGGGAAAGATTAAATCAATATGCAAATGAAACATTGACGGAAGAAGATTTTATTCCAATTCAGCGGGTCG
ATGCCGTGTGCAAGGTAGACGAATTGACGGTTCAAGCGATTGAAGAAGCGGGGATGCTGTCACCGTTTGGTATGTCAAAC
CCAAAACCGTTCATTATGATTGAAGATGCGAAGCTTGAAGATATCCGAACGATTGGGGCAAATCACAACCATATTAAAAT
GTCTCTGAAAGACGAAGACACACTGCTTGACTGTGTCGGCTTTCATCAAGGGAAATTAAAAGAGGAAATTGTTCCAGGAA
GCCGCATTTCTGTGATTGGAGAAATGTCTGTCAATGAATGGAATAACCGGAAGAAGCCACAGCTGATGTTAAAAGATGCA
CGAGTTGATGAATGGCAGCTGTTTGATCTGCGCGGGAAAAAGGATTGGGAGAAGAAAGTTTCGACACTTGATTCAGAAAA
ATCATTGGTTGTCTGTTTTGATGAAGCAACAAAACAGGACGTCGATCTCCCCATTCATCATGTTCATGCAGAAAATGTAT
CCACATTTGATGTACAAGGAAAGTATGTGGTCTTTGCGGATGTTCCCGCAGATGAGCAATTGCTGAAGCAGCTTTTAGAA
AATCAAAAGCCTGCGAGAATTTATACGGTTTTTCAAAGAAAAGAAGATCATTTTATGTCCTCTTTCCCAAGCCGTGACCA
ATTCAAATGGTTTTATGGGTTCTTGTTTAAACGGGGAAGTTTTCCTATCAAAGAACAAGGAATGGAGCTTGCCAAACATA
GAGGCTGGACAAAAGATACAATCATTTTTATGACAAAGGTGTTTTTTGAACTCGGTTTTGTTAAAATAGAGAATGGTGTG
CTGTCAATAGTACTTGATGCCCCTAAAAAAGATTTAACGGCGTCAGCGTCCTATACAGCAAAACAACGATTGATGGAACT
CGATCAAACACTAACATATTCCTCAGCTAAAGAGCTGAAGGAATGGCTGAATAGCATGATGAGCGAAGTCTCACCTGTGC
TTTGA

Domains

Predicted by InterproScan.

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(566-763)

DHH family (DHH)

(83-226)

RecJ OB domain (RecJ_OB)

(453-559)

DHHA1 domain (DHHA1)

(311-437)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

67.439

100

0.677

Multiple sequence alignment