Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   MHI23_RS12490 Genome accession   NZ_CP152024
Coordinates   2430161..2432485 (-) Length   774 a.a.
NCBI ID   WP_342491990.1    Uniprot ID   -
Organism   Bacillus sp. FSL M7-0003     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2425161..2437485
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHI23_RS12475 (MHI23_12475) dtd 2426810..2427253 (-) 444 WP_024427859.1 D-aminoacyl-tRNA deacylase -
  MHI23_RS12480 (MHI23_12480) - 2427272..2429482 (-) 2211 WP_024427858.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
  MHI23_RS12485 (MHI23_12485) - 2429625..2430137 (-) 513 WP_024423133.1 adenine phosphoribosyltransferase -
  MHI23_RS12490 (MHI23_12490) recJ 2430161..2432485 (-) 2325 WP_342491990.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  MHI23_RS12495 (MHI23_12495) - 2432567..2432917 (-) 351 WP_024423135.1 lipopolysaccharide assembly protein LapA domain-containing protein -
  MHI23_RS12500 (MHI23_12500) - 2433019..2433507 (-) 489 WP_024423136.1 cation:proton antiporter regulatory subunit -
  MHI23_RS12505 (MHI23_12505) secDF 2433890..2436118 (-) 2229 WP_024423137.1 protein translocase subunit SecDF -
  MHI23_RS12510 (MHI23_12510) - 2436160..2436456 (-) 297 WP_003216788.1 post-transcriptional regulator -

Sequence

Protein


Download         Length: 774 a.a.        Molecular weight: 87044.53 Da        Isoelectric Point: 4.8553

>NTDB_id=985459 MHI23_RS12490 WP_342491990.1 2430161..2432485(-) (recJ) [Bacillus sp. FSL M7-0003]
MLLSKMRWEYEHPSEEKVKSLSENLNISALTASLLVKRGFEEVEEARSFLFDQKAEFHDPFLLKGMKEAVERINKAIEEQ
ESIVIFGDYDADGVTSTSVLLHTLKERSAIVDFYIPDRFKEGYGPNEQAFRYIKEQGASLVITVDTGIAAVNEARIAKEI
GLDLIITDHHEPSEELPEALAIVHPKQPGCEYPFKELAGVGVAFKVAHALLGELPVQLLDLAAIGTIADLVPLHDENRWL
AKKGLMQLRQSNRPGLKALLKEAGATLEEASEETVGFQIAPRLNAVGRIEQADPAVHLLMTEDKDEAEELARFVQELNKE
RQKIVSTITEEAIQMVEEAGDDQSAIVVAKAGWNPGVVGIVASKLTDTFSRPAIVLGIDEETQMAKGSARSIPGFDLFFH
LSKCRDILPHFGGHPMAAGMTLRADDVSLLRERLNQYANETLTEEDFIPIQRVDAVCKVDELTVQAIEEAGMLSPFGMSN
PKPFIMIEDAKLEDIRTIGANHNHIKMSLKDEDTLLDCVGFHQGKLKEEIVPGSRISVIGEMSVNEWNNRKKPQLMLKDA
RVDEWQLFDLRGKKDWEKKVSTLDSENSLVVCFDETTKQDVDLPIHHVHAENVSTFDVQGKYVVFADVPADEQLLKQLLE
NQKPARIYTVFQRKEDHFMSSFPSRDQFKWFYGFLFKRGSFPIKEQGMELAKHRGWTKDTIIFMTKVFFELGFVKIENGV
LSIVLDAPKKDLTASASYTAKQRLMELDQTLTYSSAKELKEWLNSMMSEVSPVL

Nucleotide


Download         Length: 2325 bp        

>NTDB_id=985459 MHI23_RS12490 WP_342491990.1 2430161..2432485(-) (recJ) [Bacillus sp. FSL M7-0003]
ATGTTATTATCCAAAATGCGCTGGGAATATGAACATCCAAGCGAAGAAAAAGTGAAATCACTCTCTGAAAATCTAAATAT
TTCAGCGCTGACTGCATCGCTTCTCGTAAAAAGAGGATTTGAAGAAGTAGAAGAGGCGAGGTCTTTTTTATTTGATCAAA
AAGCCGAATTTCATGATCCATTCCTATTAAAAGGAATGAAAGAAGCAGTAGAGCGCATCAACAAAGCGATCGAAGAGCAG
GAATCGATTGTGATCTTCGGAGACTATGATGCAGATGGTGTGACAAGTACGTCTGTACTTTTACATACATTAAAAGAGCG
GTCTGCGATAGTAGACTTTTATATTCCAGATCGATTTAAAGAAGGCTATGGACCTAATGAACAGGCTTTTCGATATATCA
AGGAGCAAGGAGCATCGCTTGTCATCACGGTCGATACAGGCATTGCTGCTGTAAATGAAGCACGTATTGCAAAAGAAATA
GGGTTAGACCTGATCATTACAGATCACCATGAACCAAGTGAGGAGCTGCCTGAGGCACTGGCCATTGTCCATCCTAAGCA
GCCTGGCTGTGAATATCCCTTTAAAGAACTTGCTGGTGTAGGTGTCGCGTTTAAAGTGGCGCATGCACTTCTTGGAGAGC
TGCCAGTTCAGCTGTTAGATCTTGCAGCAATCGGGACAATCGCGGATCTTGTACCGCTTCACGATGAGAATAGATGGCTC
GCCAAAAAAGGCCTTATGCAGCTTCGGCAGTCGAATAGACCGGGGCTGAAAGCGTTATTGAAAGAAGCTGGAGCCACTCT
AGAAGAAGCGAGCGAGGAAACGGTCGGCTTTCAAATTGCGCCAAGGCTGAATGCTGTTGGGCGAATTGAACAGGCAGATC
CAGCCGTTCATCTTCTAATGACTGAGGATAAGGATGAAGCGGAAGAGCTCGCTCGTTTTGTACAGGAACTGAATAAAGAA
CGACAAAAGATTGTCAGTACGATCACAGAAGAAGCCATTCAAATGGTGGAAGAGGCAGGTGATGACCAGTCTGCGATTGT
TGTAGCGAAAGCTGGCTGGAATCCGGGCGTTGTCGGAATTGTTGCCTCTAAATTGACGGATACTTTTTCTCGTCCGGCGA
TTGTGCTTGGAATAGATGAAGAGACGCAAATGGCAAAAGGATCAGCCCGGAGTATTCCTGGCTTCGATCTATTTTTCCAT
CTAAGCAAATGTCGCGACATCCTGCCGCATTTCGGCGGGCATCCGATGGCAGCAGGGATGACACTTCGAGCGGATGATGT
ATCTTTACTAAGGGAAAGATTAAATCAATATGCAAATGAAACATTGACGGAAGAAGATTTTATTCCAATTCAGCGGGTCG
ATGCCGTGTGCAAGGTAGACGAATTGACGGTTCAAGCGATTGAAGAAGCGGGGATGCTGTCACCATTTGGTATGTCAAAC
CCAAAACCGTTTATTATGATTGAAGATGCGAAGCTTGAAGATATCCGAACGATTGGGGCAAATCACAACCATATTAAAAT
GTCTCTGAAAGACGAAGACACACTGCTTGACTGTGTTGGCTTTCACCAAGGGAAATTAAAAGAGGAAATTGTTCCAGGAA
GCCGCATTTCTGTGATTGGAGAAATGTCTGTCAATGAATGGAATAACCGGAAGAAGCCCCAGCTGATGTTAAAAGATGCA
CGAGTTGATGAATGGCAGCTGTTTGATCTGCGCGGGAAAAAGGATTGGGAGAAGAAAGTTTCGACACTTGATTCAGAAAA
TTCATTGGTTGTCTGTTTTGATGAAACAACAAAACAGGACGTCGATCTCCCCATTCATCATGTTCATGCAGAAAATGTAT
CAACATTTGATGTACAAGGAAAGTATGTGGTCTTTGCGGATGTTCCCGCAGATGAGCAATTGCTGAAGCAGCTTTTAGAA
AATCAAAAGCCTGCGAGAATTTATACGGTTTTTCAAAGAAAAGAAGATCATTTTATGTCCTCTTTCCCAAGCCGTGACCA
ATTCAAATGGTTTTATGGGTTCTTGTTTAAACGGGGAAGTTTTCCTATTAAAGAACAAGGAATGGAGCTTGCCAAACATA
GAGGCTGGACAAAAGATACAATCATTTTTATGACAAAGGTGTTTTTTGAACTCGGTTTTGTTAAAATAGAGAATGGTGTG
CTGTCAATAGTACTTGATGCCCCTAAAAAAGATTTAACGGCGTCAGCGTCCTATACAGCAAAACAACGATTGATGGAACT
CGATCAAACACTAACATATTCCTCAGCTAAAGAGCTGAAGGAATGGCTGAATAGCATGATGAGCGAAGTCTCACCTGTGC
TTTGA

Domains

Predicted by InterproScan.

DHH family (DHH)

(83-226)

RecJ OB domain (RecJ_OB)

(453-559)

DHHA1 domain (DHHA1)

(311-437)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(566-763)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

67.696

100

0.68

Multiple sequence alignment