Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   MHI41_RS12430 Genome accession   NZ_CP152023
Coordinates   2425511..2427844 (-) Length   777 a.a.
NCBI ID   WP_342494224.1    Uniprot ID   -
Organism   Bacillus sp. FSL M7-0307     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2420511..2432844
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHI41_RS12415 (MHI41_12415) dtd 2422160..2422603 (-) 444 WP_008342725.1 D-aminoacyl-tRNA deacylase -
  MHI41_RS12420 (MHI41_12420) - 2422622..2424832 (-) 2211 WP_007501480.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
  MHI41_RS12425 (MHI41_12425) - 2424975..2425487 (-) 513 WP_008342729.1 adenine phosphoribosyltransferase -
  MHI41_RS12430 (MHI41_12430) recJ 2425511..2427844 (-) 2334 WP_342494224.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  MHI41_RS12435 (MHI41_12435) - 2427926..2428282 (-) 357 WP_286108431.1 lipopolysaccharide assembly protein LapA domain-containing protein -
  MHI41_RS12440 (MHI41_12440) - 2428382..2428870 (-) 489 WP_008342734.1 cation:proton antiporter regulatory subunit -
  MHI41_RS12445 (MHI41_12445) secDF 2429071..2431299 (-) 2229 WP_342494225.1 protein translocase subunit SecDF -
  MHI41_RS12450 (MHI41_12450) - 2431341..2431637 (-) 297 WP_003216788.1 post-transcriptional regulator -

Sequence

Protein


Download         Length: 777 a.a.        Molecular weight: 87645.60 Da        Isoelectric Point: 4.8062

>NTDB_id=985399 MHI41_RS12430 WP_342494224.1 2425511..2427844(-) (recJ) [Bacillus sp. FSL M7-0307]
MLLSKMRWEYEHPSEEKVKSLSENLNISALTASLLVQRGLEEVEEARSFLFDQKAEFHDPFLLKGMKEAVERINKAIEEQ
ESIVIFGDYDADGVTSTSVLLHTLKERSAKVDFYIPDRFKEGYGPNEQAFRYIKEQGASLVITVDTGIAAVNEARIAKEI
GLDLIITDHHEPSEELPEALAIVHPKQPGCEYPFKELAGVGVAFKVAHALLGKLPVELLDLAAIGTIADLVPLLDENRWL
AKKGLMQLRQSRRPGLKALLKEAGATLEEANEETIGFQIAPRLNAVGRIEQADPAVHLLMTEDMDEAEELARFVQELNKE
RQKIVSTITEEAIQMVEETGADQSAIVVAKAGWNPGVVGIVASKLTDTFSRPAIVLGIDEETQMAKGSARSIPGFDLFFH
LSKCRDILPHFGGHPMAAGMTLQADDVPLLRERLNQYANETLTEEDFIPIQRVDAVCKVDEMTVQAIEEVGLLSPFGMQN
PKPFIMIEDAKLEDIRTIGANQNHIKMSLKDEDKLLDCVGFHQGKLKEELVPGSLISVVGEVSINEWNNRKKPQLMLKDA
RVDEWQLFDLRGKKDWEKKVLTLDPAKTMVFCFDEETKRQSELVDIPIHLIDAENVSTFDVQEKYVIFADVPADEHLLKL
LLENQQPARIYTVFQRKEDHFMSSFPSRDQFKWFYGFLFKRGSFPIKEQGMELAKHRGWTKDTIIFMTKVFFELGFVKIE
NGVLSIVRDAPKKDLTASSSYTAKQRLMELDQTLTYSSAKELKEWLNSMMSEVSPVL

Nucleotide


Download         Length: 2334 bp        

>NTDB_id=985399 MHI41_RS12430 WP_342494224.1 2425511..2427844(-) (recJ) [Bacillus sp. FSL M7-0307]
ATGTTATTATCCAAAATGCGTTGGGAATATGAACATCCTAGCGAAGAAAAAGTGAAATCACTCTCTGAAAATCTGAATAT
TTCAGCGCTGACTGCATCACTTCTTGTACAAAGAGGGCTCGAAGAAGTAGAAGAAGCGAGGTCTTTTTTATTTGATCAAA
AAGCCGAATTTCATGATCCATTCTTATTAAAAGGAATGAAAGAAGCAGTAGAGCGCATCAACAAAGCGATCGAAGAGCAG
GAATCGATTGTGATCTTCGGAGATTATGATGCAGATGGTGTGACAAGTACGTCTGTACTTTTACATACACTAAAAGAACG
ATCTGCTAAGGTAGACTTTTATATTCCAGATCGATTTAAAGAAGGATATGGGCCAAACGAACAGGCTTTCCGATATATCA
AAGAGCAAGGGGCATCGCTTGTTATCACCGTAGATACAGGGATCGCTGCTGTAAATGAAGCACGTATTGCAAAAGAAATA
GGATTAGACCTGATCATCACAGATCACCATGAACCAAGCGAGGAACTGCCTGAGGCACTTGCGATTGTACATCCTAAGCA
GCCTGGATGTGAGTATCCGTTTAAAGAGCTTGCGGGTGTCGGTGTTGCGTTTAAAGTGGCACATGCACTCCTTGGTAAAC
TCCCAGTAGAATTATTAGATCTTGCTGCAATCGGTACAATTGCCGATCTTGTCCCGCTGCTTGATGAAAATAGATGGCTT
GCAAAAAAAGGTCTGATGCAGCTTCGTCAATCTAGACGGCCTGGGTTAAAAGCGTTATTAAAAGAAGCAGGCGCCACTCT
TGAAGAAGCAAATGAGGAAACGATCGGCTTTCAAATTGCGCCAAGGCTGAATGCTGTTGGACGAATTGAGCAGGCAGATC
CGGCTGTTCATCTTCTCATGACTGAGGATATGGATGAAGCAGAAGAACTCGCTCGTTTTGTACAAGAATTGAACAAAGAA
CGGCAGAAAATTGTGAGTACGATCACGGAAGAAGCCATTCAAATGGTTGAAGAGACAGGCGCTGACCAGTCTGCTATCGT
CGTAGCAAAAGCTGGCTGGAATCCAGGCGTTGTTGGCATTGTTGCCTCAAAATTGACGGATACATTTTCTCGTCCGGCGA
TTGTGCTTGGAATAGACGAAGAAACGCAGATGGCGAAAGGGTCAGCACGAAGTATTCCTGGCTTTGATTTATTTTTCCAT
TTGAGCAAGTGCCGTGACATCCTGCCGCATTTCGGTGGACACCCGATGGCAGCAGGAATGACACTTCAAGCGGATGATGT
TCCTTTACTTAGGGAAAGATTGAATCAATATGCAAATGAAACGTTGACAGAAGAAGATTTTATTCCAATTCAACGGGTCG
ATGCAGTATGTAAAGTGGACGAAATGACGGTGCAAGCCATTGAAGAAGTGGGGCTGCTGTCCCCGTTTGGCATGCAGAAT
CCTAAGCCGTTCATTATGATTGAAGACGCCAAGCTTGAAGACATCCGAACGATCGGGGCCAATCAAAATCACATTAAAAT
GTCACTAAAAGACGAAGACAAGCTGCTCGACTGTGTCGGTTTTCACCAAGGCAAACTAAAAGAAGAACTTGTCCCGGGGA
GTCTAATTTCTGTCGTTGGCGAGGTCTCGATTAATGAATGGAATAACCGAAAGAAACCTCAGCTCATGCTAAAAGACGCT
AGAGTCGATGAATGGCAGCTGTTTGATTTGCGCGGGAAAAAAGATTGGGAAAAGAAAGTATTAACACTTGATCCAGCAAA
AACGATGGTCTTCTGTTTTGACGAAGAAACAAAAAGGCAATCGGAGCTAGTGGATATTCCGATTCATCTTATTGATGCAG
AAAATGTATCCACGTTTGATGTGCAGGAAAAGTATGTGATCTTTGCCGATGTTCCTGCCGATGAACACTTGCTCAAGCTG
CTTTTAGAAAACCAACAACCAGCAAGAATTTATACGGTGTTTCAGCGGAAAGAAGATCACTTTATGTCCTCATTCCCGAG
CCGTGATCAATTCAAATGGTTTTATGGGTTCTTGTTCAAACGGGGAAGTTTTCCTATTAAAGAACAAGGCATGGAGCTTG
CCAAACATAGAGGTTGGACAAAAGATACAATCATTTTTATGACAAAGGTGTTTTTTGAACTCGGTTTTGTTAAAATAGAG
AATGGTGTGCTGTCAATAGTGCGTGATGCCCCTAAAAAAGATTTAACCGCATCATCGTCTTATACAGCAAAACAACGATT
GATGGAACTCGATCAAACACTAACATATTCCTCAGCCAAAGAGCTGAAGGAATGGCTGAATAGCATGATGAGCGAAGTCT
CACCTGTGCTTTGA

Domains

Predicted by InterproScan.

RecJ OB domain (RecJ_OB)

(453-559)

DHHA1 domain (DHHA1)

(314-437)

DHH family (DHH)

(83-226)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(566-766)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

66.795

100

0.668

Multiple sequence alignment