Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   MHI08_RS05160 Genome accession   NZ_CP152022
Coordinates   1027164..1030673 (+) Length   1169 a.a.
NCBI ID   WP_342500055.1    Uniprot ID   -
Organism   Bacillus sp. FSL M7-0791     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1022164..1035673
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHI08_RS05130 (MHI08_05130) lepB 1022684..1023196 (+) 513 WP_234107696.1 signal peptidase I -
  MHI08_RS05135 (MHI08_05135) - 1023235..1024380 (-) 1146 WP_208946661.1 AbrB family transcriptional regulator -
  MHI08_RS05140 (MHI08_05140) - 1024647..1025069 (+) 423 WP_024425495.1 BlaI/MecI/CopY family transcriptional regulator -
  MHI08_RS05145 (MHI08_05145) - 1025062..1025901 (+) 840 WP_342500053.1 M56 family metallopeptidase -
  MHI08_RS05150 (MHI08_05150) - 1025959..1026378 (+) 420 WP_342500054.1 DoxX family protein -
  MHI08_RS05155 (MHI08_05155) - 1026565..1027011 (-) 447 WP_044334155.1 ferritin-like domain-containing protein -
  MHI08_RS05160 (MHI08_05160) addB 1027164..1030673 (+) 3510 WP_342500055.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  MHI08_RS05165 (MHI08_05165) addA 1030651..1034355 (+) 3705 WP_342500056.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  MHI08_RS05170 (MHI08_05170) sbcD 1034382..1035557 (+) 1176 WP_248351368.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1169 a.a.        Molecular weight: 134919.02 Da        Isoelectric Point: 5.4378

>NTDB_id=985311 MHI08_RS05160 WP_342500055.1 1027164..1030673(+) (addB) [Bacillus sp. FSL M7-0791]
MEIQFLAGRSGSGKTTAILEEIKEQLRLDPLGPPIIFLVPDQMTFLMEYELAKTSEAGGMIRAKVFSFTRLAWSILQQTG
GANRQFVTSTGIQMLLRKVIEEEKEKFKVFKKASDKPGFVEQIEKTMAEFKRYCMLPEDIEKISLTSMHSEYTEERRAAE
KLHDLHVLYQQMEQHLQDEYVHSEDYLTLLTQQIPLSEEIKGAHIYIDGFYQFTPQQLLVIEQLLLHAEKITAAFTVDRS
YHDKQPNELDLFRMTGKTYFQLYQLAKECGAAISEHILERNNRHLHTPDLAYLERQYDQRPVQPYQEVTPHLTVSKSASK
RAEIEGVAREILHLVREEGLRLRDISIVARHVDDYKDTLKEVFRDYDIPFFIDGNESMQYHPLIELIRSSLDVIKGNWRY
EAVFRCVKTEFLFPLELAKNKAREQADQLENYCIAYGVKGERWTNGSRFHYRRFQSLDEDFGQTDQEIEMEQMLNDVKEW
IAPPLFQLQKRLKKAQKVKDMVEAVYVFLEEIQVPDKLEKARLEAEEAGRLAEAMQHGQVWEAVIQLMDEFVEMLGEEEL
SFPLFQQMMDTGLASLKFALIPPSLDQVFIGSMDLSRMYQVKCMFIIGVNDGVIPARPSDESVLSEDDREWLKRAGAELA
ETGKERLLDEQFLIYQALSSPSHHLYLSYSVSDEEGRSLLPSTLIKYCQELMPNHEHALYVLDPEQLDDKEQLKFVANEH
VSLSYTVSQLQQWLNQYPISGVWWSVYNYLMTSSHRDVSKNILSSLFFTNRAKRLKPNVTKELYGDHIQGSVSRMEKFNA
CAFSHFASHGLKLKDRQFYKLEAPDIGQLFHSALKHISDTLVEQKKDWKNLTKDDCVNYSRHAIEQLAPRLQKEILLSSN
RHAYIKEKLQKILIRVSSILSEHARVSGFSPVGLELGFGGQGPLPPFTFQLKNGCTMELVGRIDRVDKAEGSKGLLLRIV
DYKSSEKGLDLAEVYYGLALQMLTYLDLTITYSKEWLGVEATPAGILYFHIHDPLIQAPIPLAEDEIEQEIFKKFKMKGL
LLEDVEAVKLMDQTLESGRSQVIQAGLKKDGSFRSDSAVLSEDHFHILTQHVRRTFEEAGERITNGEVAINPYKLKDQTP
CRFCSFKSICQFDESIEDNEFRVLSSEKDDVVIDRIKKEGDQYANTKTE

Nucleotide


Download         Length: 3510 bp        

>NTDB_id=985311 MHI08_RS05160 WP_342500055.1 1027164..1030673(+) (addB) [Bacillus sp. FSL M7-0791]
GTGGAGATTCAGTTTTTAGCAGGCAGATCGGGGAGTGGAAAAACGACTGCAATCTTAGAGGAAATCAAAGAACAGCTTCG
GCTTGATCCGTTGGGTCCGCCAATCATTTTTTTAGTCCCGGATCAAATGACATTCTTAATGGAATACGAGCTTGCAAAAA
CGTCTGAAGCTGGCGGGATGATCAGGGCCAAAGTATTTAGTTTCACACGTCTTGCTTGGTCTATTTTACAGCAGACTGGC
GGAGCGAACCGGCAATTTGTGACAAGCACAGGAATTCAAATGCTTTTAAGAAAAGTGATTGAAGAGGAGAAAGAAAAGTT
TAAGGTATTCAAAAAAGCGAGTGATAAGCCGGGGTTTGTGGAGCAAATCGAAAAGACGATGGCTGAGTTCAAGAGATACT
GCATGCTGCCGGAGGATATTGAAAAAATTTCATTAACGAGCATGCATTCAGAGTATACAGAAGAAAGACGGGCAGCCGAG
AAATTGCATGATCTGCATGTTCTTTATCAGCAGATGGAGCAGCATTTACAAGATGAATATGTGCACTCAGAAGACTATTT
GACACTTCTCACGCAGCAAATTCCTTTATCTGAAGAAATAAAAGGAGCACACATTTATATAGATGGTTTTTATCAATTTA
CACCGCAGCAGCTTCTCGTCATTGAACAGCTTCTGCTGCATGCTGAGAAAATAACGGCTGCTTTCACAGTGGATCGTTCC
TATCATGATAAGCAGCCAAATGAACTTGATTTATTTCGTATGACAGGTAAAACGTATTTCCAGCTCTATCAGCTTGCCAA
AGAGTGCGGAGCGGCCATTTCTGAGCACATTTTGGAGAGAAATAACCGTCATCTTCATACGCCGGATCTTGCTTATTTAG
AACGGCAATATGATCAGAGACCGGTGCAGCCATATCAGGAAGTCACTCCTCATCTCACAGTATCTAAAAGTGCGAGTAAA
CGAGCTGAAATTGAGGGAGTAGCGAGAGAAATTCTTCATTTAGTGAGAGAAGAAGGACTTAGACTGCGAGATATTTCGAT
TGTAGCGCGGCATGTAGACGACTATAAAGATACGTTAAAAGAGGTTTTTCGAGATTACGATATTCCTTTTTTTATTGATG
GAAATGAATCGATGCAGTATCATCCGCTCATTGAATTGATTCGTTCGAGCTTGGATGTCATAAAAGGAAACTGGCGGTAT
GAGGCCGTGTTTCGATGCGTGAAGACAGAGTTCTTATTTCCGCTCGAACTTGCCAAAAACAAAGCGAGAGAACAGGCAGA
TCAGCTTGAAAACTACTGTATTGCTTATGGTGTAAAAGGAGAACGCTGGACAAACGGTTCCCGTTTCCATTACAGGCGGT
TCCAATCCTTAGATGAGGATTTTGGACAAACAGATCAAGAAATTGAAATGGAACAGATGCTGAATGATGTAAAGGAATGG
ATCGCACCTCCGCTTTTCCAATTGCAAAAAAGGCTGAAAAAAGCGCAAAAGGTAAAAGACATGGTAGAGGCAGTCTATGT
CTTTTTAGAGGAAATACAAGTGCCGGATAAACTTGAAAAGGCTAGGCTGGAAGCTGAAGAAGCAGGTAGATTAGCTGAAG
CCATGCAGCATGGACAAGTATGGGAAGCTGTTATTCAATTAATGGACGAATTTGTGGAAATGCTCGGTGAGGAGGAGCTC
TCATTTCCTTTATTCCAACAGATGATGGATACAGGATTGGCCTCTTTAAAATTCGCTTTAATTCCGCCTTCACTCGACCA
AGTATTTATCGGAAGTATGGATTTATCCAGAATGTATCAAGTGAAGTGTATGTTTATCATTGGGGTAAATGATGGCGTTA
TTCCCGCACGCCCTTCTGACGAAAGTGTATTGTCTGAGGATGACAGGGAATGGTTAAAGCGAGCAGGAGCAGAGCTGGCA
GAGACAGGAAAGGAACGGCTGCTAGATGAGCAATTTTTAATTTACCAGGCGCTGTCGAGTCCATCTCATCATTTGTATCT
GTCTTATTCAGTGTCAGATGAAGAGGGGCGCTCTCTATTGCCTTCGACTCTTATCAAGTATTGTCAGGAGCTTATGCCAA
ATCATGAGCATGCTTTGTATGTGTTAGACCCGGAACAGCTGGATGACAAGGAGCAATTAAAATTTGTAGCGAATGAGCAT
GTCTCGTTATCGTATACTGTCTCGCAGCTGCAGCAATGGCTCAACCAATATCCCATTAGTGGTGTATGGTGGAGTGTCTA
TAATTATTTGATGACGTCATCTCATCGGGATGTATCAAAAAACATCTTGTCGAGTTTGTTTTTTACAAATCGAGCGAAGC
GCTTAAAACCAAACGTCACAAAAGAACTTTATGGTGATCACATTCAAGGCAGTGTTTCAAGAATGGAGAAATTCAATGCG
TGTGCATTTTCTCACTTTGCTTCCCACGGGTTAAAACTAAAGGACCGGCAATTTTATAAATTGGAAGCACCCGATATCGG
CCAGCTGTTTCACTCGGCCTTAAAGCATATTTCAGATACGCTTGTTGAACAAAAAAAGGATTGGAAAAACCTAACGAAGG
ATGACTGTGTCAACTATTCAAGACATGCAATCGAACAGCTTGCACCGCGTCTTCAAAAGGAAATCCTATTAAGCTCTAAC
CGGCATGCTTATATTAAAGAAAAGCTCCAGAAAATCTTGATTCGAGTCTCTTCTATATTAAGTGAGCATGCAAGAGTGAG
TGGATTTTCTCCCGTTGGGCTTGAGTTAGGCTTTGGCGGCCAAGGACCACTTCCACCGTTTACTTTTCAATTGAAAAATG
GCTGTACGATGGAGCTTGTCGGAAGAATTGACCGGGTGGATAAAGCAGAAGGTTCAAAAGGGCTGCTGTTGAGAATCGTC
GACTACAAATCGAGTGAGAAAGGTCTTGATCTAGCAGAAGTATACTATGGACTGGCCTTGCAAATGCTCACTTACTTAGA
CCTGACCATTACGTATTCGAAGGAGTGGCTCGGCGTAGAAGCAACACCAGCTGGCATTTTATATTTCCATATTCATGATC
CGCTCATTCAAGCACCGATTCCGCTTGCAGAGGATGAAATTGAACAGGAAATATTCAAGAAATTTAAAATGAAAGGTCTA
TTGCTTGAAGATGTAGAAGCGGTAAAACTAATGGATCAAACGCTTGAATCAGGAAGATCACAAGTCATTCAAGCCGGCTT
GAAAAAAGATGGGTCCTTCCGTTCTGACTCTGCTGTTTTAAGTGAAGATCATTTCCATATACTCACACAGCATGTTCGGC
GCACGTTTGAAGAGGCAGGCGAAAGAATTACAAATGGCGAAGTAGCCATTAATCCATATAAATTGAAGGATCAAACACCA
TGCCGCTTTTGTTCATTTAAGTCTATTTGCCAATTTGATGAATCGATAGAGGATAATGAATTTAGAGTGCTTTCATCTGA
AAAGGATGATGTTGTGATAGACCGGATCAAAAAAGAAGGGGATCAGTATGCAAATACCAAAACCGAATAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

65.719

99.316

0.653

Multiple sequence alignment