Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   NST65_RS12200 Genome accession   NZ_CP151983
Coordinates   2382784..2385108 (-) Length   774 a.a.
NCBI ID   WP_111926397.1    Uniprot ID   -
Organism   Bacillus sp. FSL P4-0248     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2377784..2390108
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NST65_RS12185 (NST65_12185) dtd 2379434..2379877 (-) 444 WP_034279904.1 D-aminoacyl-tRNA deacylase -
  NST65_RS12190 (NST65_12190) - 2379896..2382106 (-) 2211 WP_034279902.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
  NST65_RS12195 (NST65_12195) - 2382248..2382760 (-) 513 WP_034279900.1 adenine phosphoribosyltransferase -
  NST65_RS12200 (NST65_12200) recJ 2382784..2385108 (-) 2325 WP_111926397.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  NST65_RS12205 (NST65_12205) - 2385190..2385540 (-) 351 WP_111926395.1 lipopolysaccharide assembly protein LapA domain-containing protein -
  NST65_RS12210 (NST65_12210) - 2385642..2386130 (-) 489 WP_041115109.1 cation:proton antiporter regulatory subunit -
  NST65_RS12215 (NST65_12215) secDF 2386317..2388545 (-) 2229 WP_034279892.1 protein translocase subunit SecDF -
  NST65_RS12220 (NST65_12220) - 2388587..2388883 (-) 297 WP_003216788.1 post-transcriptional regulator -

Sequence

Protein


Download         Length: 774 a.a.        Molecular weight: 87002.61 Da        Isoelectric Point: 4.9495

>NTDB_id=983380 NST65_RS12200 WP_111926397.1 2382784..2385108(-) (recJ) [Bacillus sp. FSL P4-0248]
MLLSKMRWEYEHPSEEKVKSLSENLNISALTASLLVKRGLEEVEEARSFLFDQKAEFHDPFLLKGMKESVERINKAIEEQ
ESIVIFGDYDADGVTSTSVLLHTLKERSAIVDFYIPDRFKEGYGPNEQAFRYIKEQGASLVITVDTGIAAVNEARIAKEI
GLDLIITDHHEPSEELPEALAIVHPKQPGCEYPFKELAGVGVAFKVAHALLGKLPVQLLDLAAIGTIADLVPLHDENRWL
AKKGLVQLRQSNRPGLKALLKEAGATLEEANEETVGFQIAPRLNAVGRIEQADPAVHLLMTEDKDEAEELARFVQELNKE
RQKIVSTITEEAIQMVEEAGDDQSAIVVAKAGWNPGVVGIVASKLTDTFSRPAIVLGIDEETQMAKGSARSIPGFDLFFH
LSKCRDILPHFGGHPMAAGMTLRADDVALLRERLNQYANETLTEEDFIPIQRVDAVCKVDELTVEAIEEAGMLSPFGMSN
PKPFIMIEDAKLEDIRTIGANHNHIKMSLKDEDKLLDCVGFHQGKLKEEIVPGSRISVIGEMSVNEWNNRKKPQLMLKDA
RVDEWQLFDLRGKKDWEKKVSTLDSEKSLVVCFDEATKQDVDIPIHHVHAENVSTFDVQGKYVVFADVPADEQLLKQLLE
NQKPARIYTVFQRKEDHFMSSFPSRDQFKWFYGFLFKRGSFPIKEQGMDLAKHRGWTKDTIIFMTKVFFELGFVKIENGV
LSIVLDAPKKDLTASASYTAKQRLMELDQTLTYSSAKELKEWLNSMMSEVSPVL

Nucleotide


Download         Length: 2325 bp        

>NTDB_id=983380 NST65_RS12200 WP_111926397.1 2382784..2385108(-) (recJ) [Bacillus sp. FSL P4-0248]
ATGTTATTATCCAAAATGCGCTGGGAATATGAACATCCAAGCGAAGAAAAAGTGAAATCACTCTCTGAAAATCTGAATAT
TTCAGCGCTGACTGCATCGCTTCTCGTAAAAAGAGGACTTGAAGAAGTAGAAGAGGCGAGGTCTTTTTTATTTGATCAAA
AAGCCGAATTCCATGATCCATTTCTATTAAAAGGAATGAAAGAATCAGTAGAGCGCATCAACAAAGCGATCGAAGAGCAG
GAATCGATTGTGATCTTCGGAGACTATGATGCAGATGGTGTGACAAGTACGTCTGTACTTTTACATACATTAAAAGAGCG
GTCTGCGATAGTAGACTTTTATATTCCAGATCGATTTAAAGAAGGCTATGGGCCTAATGAACAGGCATTTCGATATATCA
AGGAGCAAGGAGCATCGCTTGTCATCACGGTCGATACAGGCATTGCTGCTGTAAATGAAGCACGTATTGCAAAAGAAATA
GGGTTAGACCTAATCATTACAGATCACCATGAACCAAGTGAGGAGCTGCCTGAGGCACTTGCTATTGTCCATCCTAAGCA
GCCTGGCTGTGAGTATCCCTTTAAAGAACTTGCTGGTGTAGGCGTAGCGTTTAAAGTGGCGCATGCACTGCTTGGGAAGC
TGCCAGTTCAGCTGTTAGATCTTGCAGCAATCGGGACAATTGCGGACCTTGTACCGCTTCATGATGAGAATAGGTGGCTC
GCCAAAAAAGGCCTTGTGCAGCTTCGGCAGTCGAATAGACCGGGGCTGAAAGCATTATTGAAAGAAGCGGGAGCCACTCT
AGAAGAAGCCAATGAGGAAACGGTCGGCTTTCAAATTGCGCCAAGGCTGAATGCTGTAGGACGAATTGAACAGGCAGATC
CTGCCGTTCATCTTCTGATGACTGAGGATAAGGATGAAGCAGAAGAGCTCGCTCGTTTTGTACAGGAACTGAATAAAGAA
CGACAAAAGATTGTCAGTACGATCACAGAAGAAGCCATTCAAATGGTGGAAGAGGCAGGTGATGACCAGTCTGCGATCGT
TGTAGCGAAAGCTGGCTGGAATCCGGGCGTTGTCGGAATTGTTGCCTCTAAATTGACGGATACTTTTTCTCGTCCGGCGA
TTGTGCTTGGAATTGATGAAGAGACGCAAATGGCAAAAGGATCAGCCCGGAGTATTCCTGGCTTCGATCTATTTTTCCAT
CTAAGCAAATGCCGCGACATCCTGCCGCATTTCGGGGGGCACCCGATGGCAGCAGGGATGACACTTCGAGCGGATGATGT
CGCTTTACTAAGGGAAAGATTAAATCAATATGCAAATGAAACATTGACGGAAGAAGATTTTATTCCAATACAGCGGGTCG
ATGCAGTATGCAAGGTAGACGAATTGACGGTTGAAGCGATTGAGGAAGCGGGGATGCTGTCACCATTCGGTATGTCAAAC
CCAAAACCGTTCATTATGATTGAAGATGCGAAGCTTGAAGATATCCGAACGATTGGGGCAAATCACAACCATATTAAAAT
GTCTTTGAAAGACGAAGACAAGCTGCTTGACTGTGTCGGTTTTCACCAAGGGAAATTAAAAGAAGAAATTGTTCCAGGAA
GCCGCATTTCCGTGATTGGAGAAATGTCCGTCAATGAATGGAATAACCGGAAGAAGCCCCAGCTGATGCTAAAAGATGCA
CGAGTTGATGAATGGCAGCTGTTTGATCTGCGCGGGAAAAAGGATTGGGAGAAGAAAGTCTCGACACTTGATTCAGAAAA
ATCTTTGGTTGTCTGTTTTGATGAAGCAACAAAACAGGACGTCGATATCCCCATTCATCATGTTCATGCAGAAAATGTAT
CAACGTTTGATGTACAGGGCAAATATGTGGTCTTTGCGGATGTTCCCGCAGATGAGCAATTGCTGAAGCAGCTTTTAGAA
AATCAAAAGCCTGCCAGAATTTATACGGTTTTTCAAAGAAAAGAAGATCATTTTATGTCCTCTTTCCCAAGCCGTGACCA
ATTCAAATGGTTTTATGGGTTCTTGTTTAAACGGGGAAGTTTTCCTATCAAAGAACAAGGAATGGACCTTGCCAAACATA
GAGGCTGGACAAAAGATACAATCATTTTTATGACAAAGGTGTTTTTTGAACTCGGTTTTGTTAAAATAGAGAATGGTGTG
CTGTCAATAGTACTTGATGCCCCTAAAAAAGATTTAACGGCATCAGCATCCTATACAGCAAAACAACGATTGATGGAACT
CGATCAAACACTAACATATTCCTCAGCTAAAGAGCTGAAGGAATGGCTGAATAGCATGATGAGCGAAGTCTCACCTGTGC
TTTGA

Domains

Predicted by InterproScan.

DHHA1 domain (DHHA1)

(311-437)

RecJ OB domain (RecJ_OB)

(453-559)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(566-763)

DHH family (DHH)

(83-226)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

67.31

100

0.676

Multiple sequence alignment