Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   NSU11_RS05335 Genome accession   NZ_CP151981
Coordinates   1091858..1095367 (+) Length   1169 a.a.
NCBI ID   WP_342493828.1    Uniprot ID   -
Organism   Bacillus sp. FSL R5-0712     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1086858..1100367
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NSU11_RS05305 (NSU11_05305) lepB 1087375..1087887 (+) 513 WP_024425493.1 signal peptidase I -
  NSU11_RS05310 (NSU11_05310) - 1087926..1089071 (-) 1146 WP_133744063.1 AbrB family transcriptional regulator -
  NSU11_RS05315 (NSU11_05315) - 1089338..1089760 (+) 423 WP_024425495.1 BlaI/MecI/CopY family transcriptional regulator -
  NSU11_RS05320 (NSU11_05320) - 1089753..1090592 (+) 840 WP_133744020.1 M56 family metallopeptidase -
  NSU11_RS05325 (NSU11_05325) - 1090650..1091060 (+) 411 WP_024425497.1 DoxX family protein -
  NSU11_RS05330 (NSU11_05330) - 1091258..1091704 (-) 447 WP_048238419.1 ferritin-like domain-containing protein -
  NSU11_RS05335 (NSU11_05335) addB 1091858..1095367 (+) 3510 WP_342493828.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  NSU11_RS05340 (NSU11_05340) addA 1095345..1099049 (+) 3705 WP_342493829.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  NSU11_RS05345 (NSU11_05345) sbcD 1099076..1100251 (+) 1176 WP_268420709.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1169 a.a.        Molecular weight: 134786.82 Da        Isoelectric Point: 5.3891

>NTDB_id=983231 NSU11_RS05335 WP_342493828.1 1091858..1095367(+) (addB) [Bacillus sp. FSL R5-0712]
MEIQFLAGRSGSGKTTAILEEIKEQLRLDPLGPPIIFLVPDQMTFLMEYELAKTSEAGGMIRAKVFSFTRLAWSILQQTG
GANRQFVTSTGIQMLLRKVIEEEKEKFKVFKKASDKPGFVEQIEKTMAEFKRYCMLPEDIEKIYLSSMHSEYTEERRAAE
KLHDLHVLYQQMEQHLQDEYVHSEDYLTLLTQQIPLSEEIKGAHIYIDGFYQFTPQQLLVIEQLLLHAEKITAAFTVDRS
YHDKQPNELDLFRMTGKTYFQLHQLARECGAAISENILEGNNRHLHTPDLAYLERQYEQRPVQPYQEVTPHLTVSKSASK
RAEIEGVAREILHLVREEGLRLRDISIVARHVDDYKDTLKEVFRDYDIPFFIDGNESMQYHPLIELIRSSLDVIKGNWRY
EAVFRCVKTEFLFPLELAKNKAREQADQLENYCIAYGVKGERWTNGSRFHYRRFQSLDEDFGQTDQEIEMEQMLNDVKEW
IAPPLFQLQKRLKKAQKVKDMVEAVYVFLEEIQVPEKLEKARLEAEEAGRLTEAMQHGQVWDAVIQLMDEFVEMLGEEEL
SFPLFQQMMDTGLASLKFALIPPSLDQVFIGSMDLSRMYQVKCMFIIGVNDGVIPARPSDESVLSEDDREWLKRAGAELA
ETGKERLLDEQFLIYQALSSPSHHLYLSYSVSDEEGRSLLPSTLIKYCQELMPNHQHALYVLDPEQLGDDEQLKFVANEH
VSLSYTVSQLQQWLNQYPISGVWWSVYNYLMTSSHRDVSKNILSSLFFTNRAKHLKPNVTKELYGDHIQGSVSRMEKFNA
CAFSHFASHGLKLKDRQFYKLEAPDIGQLFHSALKHISDTLVEQKKDWKNLTKEECVTYSRHAIEQLAPRLQKEILLSSN
RHAYIKEKLQKILIRVSSILSEHAKVSGFSPVGLELGFGGQGPLPPFTFQLKNGCTMELVGRIDRVDKAEGSKGLLLRIV
DYKSSEKGLDLAEVYYGLALQMLTYLDLTITYSKEWLGVEATPAGILYFHIHDPLVQAPIPLAEDEIEQEIFKKFKMKGL
LLEDVEAVKLMDQTLESGRSQVIQAGLKKDGSFRSDSAVLSEDHFHILTQHVRRTFEEAGERITNGEVAINPYKLKDQTP
CRFCSFKSICQFDESIEDNEFRVLSSEKDDVVIDRIKKEGDQYANTKTE

Nucleotide


Download         Length: 3510 bp        

>NTDB_id=983231 NSU11_RS05335 WP_342493828.1 1091858..1095367(+) (addB) [Bacillus sp. FSL R5-0712]
GTGGAGATTCAGTTTTTAGCAGGCAGATCGGGGAGTGGAAAAACGACTGCAATCTTAGAGGAAATCAAAGAACAGCTTCG
GCTTGATCCGTTGGGTCCGCCAATCATTTTTTTAGTCCCGGATCAAATGACATTCTTAATGGAATACGAGCTTGCAAAAA
CGTCTGAAGCTGGCGGAATGATCAGGGCCAAAGTATTTAGTTTCACGCGTCTTGCTTGGTCTATTTTACAGCAGACTGGC
GGAGCGAACCGGCAATTTGTGACAAGCACAGGAATTCAAATGCTTTTAAGAAAAGTGATTGAAGAGGAGAAAGAAAAGTT
TAAGGTATTCAAAAAAGCAAGTGATAAGCCGGGGTTTGTGGAGCAAATCGAAAAGACGATGGCTGAGTTCAAGAGATACT
GCATGCTTCCGGAGGATATTGAAAAAATTTATTTATCGAGCATGCATTCAGAGTATACAGAAGAAAGACGGGCAGCCGAG
AAATTGCATGATCTGCATGTTCTTTATCAGCAGATGGAGCAGCATTTACAAGATGAATATGTGCACTCAGAAGACTATTT
GACGCTTCTCACGCAGCAAATTCCTTTATCTGAAGAAATAAAAGGAGCACACATTTATATAGATGGTTTTTATCAATTTA
CACCGCAGCAGCTTCTCGTCATTGAACAGCTTCTGCTGCATGCTGAGAAAATAACGGCTGCTTTCACAGTGGATCGTTCC
TATCATGATAAGCAGCCAAATGAACTTGATTTATTTCGTATGACAGGTAAAACGTATTTCCAGCTCCATCAGCTTGCAAG
AGAGTGCGGAGCGGCCATTTCTGAGAACATTTTGGAGGGAAATAACCGTCATCTTCATACGCCAGATCTTGCTTATTTAG
AACGCCAATATGAGCAGAGACCGGTGCAGCCATATCAGGAAGTAACCCCTCATCTCACAGTATCTAAAAGTGCAAGTAAA
CGAGCTGAAATTGAGGGAGTAGCGAGAGAAATTCTTCATTTAGTGAGAGAAGAAGGACTTAGACTGCGAGATATTTCGAT
TGTAGCGCGGCATGTAGACGACTATAAAGATACGTTAAAAGAGGTTTTTCGAGATTACGATATTCCATTTTTTATTGATG
GAAATGAATCAATGCAGTATCATCCGCTCATTGAATTGATTCGTTCGAGCTTGGATGTCATAAAAGGAAACTGGCGGTAT
GAGGCCGTGTTTCGCTGCGTGAAGACAGAGTTTTTATTTCCGCTCGAACTTGCCAAAAACAAAGCGAGAGAACAGGCAGA
TCAGCTTGAAAACTACTGTATTGCTTATGGTGTAAAAGGAGAACGCTGGACAAACGGCTCCCGTTTCCATTACAGACGGT
TCCAATCCTTAGATGAGGATTTTGGACAAACAGACCAAGAAATTGAAATGGAACAGATGCTGAATGATGTAAAGGAATGG
ATCGCACCCCCGCTTTTCCAGTTGCAAAAAAGGCTGAAAAAAGCACAAAAGGTCAAAGACATGGTGGAGGCAGTCTATGT
CTTTTTAGAGGAAATACAAGTGCCGGAAAAACTTGAAAAGGCGAGGCTGGAGGCTGAAGAAGCAGGTAGATTAACTGAAG
CCATGCAGCATGGACAAGTATGGGATGCTGTAATTCAATTAATGGATGAATTTGTTGAAATGCTCGGTGAAGAGGAGCTC
TCATTTCCTTTATTCCAACAGATGATGGATACAGGACTGGCCTCTCTCAAATTCGCTTTAATTCCGCCTTCACTCGACCA
AGTATTTATCGGAAGTATGGATTTATCCAGAATGTATCAAGTGAAGTGCATGTTTATCATTGGGGTAAATGATGGCGTTA
TTCCCGCACGCCCATCTGACGAAAGTGTTTTGTCTGAGGATGACCGGGAATGGTTAAAGCGAGCAGGAGCAGAGCTTGCA
GAGACAGGAAAGGAACGGTTGTTAGATGAGCAATTTTTAATTTACCAAGCGCTGTCGAGCCCATCCCATCATTTGTATCT
GTCTTATTCGGTGTCAGATGAAGAGGGGCGATCTCTATTGCCTTCGACTCTTATCAAGTATTGTCAGGAGCTTATGCCGA
ATCATCAGCATGCTTTGTATGTGTTAGACCCGGAACAGCTGGGTGACGATGAGCAATTAAAATTTGTAGCGAATGAGCAT
GTCTCGTTATCTTATACCGTCTCGCAGCTGCAGCAATGGCTCAATCAATATCCCATCAGTGGTGTATGGTGGAGTGTCTA
TAATTATTTGATGACGTCATCTCATCGGGATGTTTCAAAAAATATCTTGTCGAGTTTGTTTTTTACAAATCGAGCGAAGC
ACTTAAAACCAAACGTCACAAAAGAACTTTACGGTGATCACATTCAAGGCAGTGTTTCAAGAATGGAGAAATTCAATGCG
TGTGCGTTTTCTCACTTTGCTTCCCACGGGTTAAAACTAAAAGACCGGCAATTTTATAAACTGGAGGCCCCTGATATCGG
CCAGCTGTTTCACTCAGCTTTAAAGCATATTTCAGATACGCTTGTTGAACAAAAAAAGGATTGGAAAAACCTAACGAAGG
AAGAGTGTGTCACTTATTCAAGACATGCAATCGAACAGCTTGCTCCGCGTCTTCAAAAGGAAATTCTATTAAGCTCTAAT
CGGCATGCTTATATTAAAGAAAAGCTCCAGAAAATCTTGATTCGAGTCTCTTCTATTTTAAGTGAGCATGCAAAAGTGAG
CGGATTTTCTCCCGTTGGGCTTGAGTTAGGCTTTGGCGGTCAAGGACCTTTGCCGCCGTTTACTTTTCAATTGAAAAATG
GCTGCACGATGGAGCTTGTCGGAAGAATTGACCGGGTGGATAAAGCAGAAGGTTCAAAAGGGCTGCTGTTGAGAATCGTC
GACTACAAATCGAGTGAGAAAGGTCTTGATCTAGCAGAAGTATACTATGGGCTGGCCTTGCAAATGCTCACTTACTTAGA
CCTGACCATTACGTATTCGAAGGAATGGCTCGGCGTAGAAGCAACACCAGCTGGCATTTTATATTTCCATATTCATGATC
CACTCGTTCAAGCACCGATTCCGCTTGCAGAGGATGAAATTGAGCAAGAAATATTCAAGAAATTTAAAATGAAAGGTCTA
TTGCTTGAAGATGTAGAAGCGGTAAAGCTAATGGATCAAACGCTTGAGTCAGGAAGATCACAAGTCATTCAAGCCGGTTT
GAAAAAGGATGGGTCTTTCCGTTCTGACTCTGCTGTTTTAAGTGAAGATCATTTTCATATACTCACACAGCATGTTCGGC
GCACGTTTGAAGAGGCAGGCGAAAGAATTACAAATGGCGAGGTAGCCATTAATCCATATAAATTAAAGGATCAAACACCA
TGCCGCTTTTGTTCATTTAAGTCTATTTGCCAATTTGATGAATCGATAGAGGACAATGAATTTAGAGTGCTTTCATCTGA
AAAGGATGATGTCGTGATAGACCGGATCAAAAAAGAAGGGGATCAGTATGCAAATACCAAAACCGAATAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

66.15

99.316

0.657

Multiple sequence alignment