Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   NST24_RS05385 Genome accession   NZ_CP151977
Coordinates   1072728..1076237 (+) Length   1169 a.a.
NCBI ID   WP_342462421.1    Uniprot ID   -
Organism   Bacillus sp. FSL R5-0286     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1067728..1081237
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NST24_RS05355 (NST24_05355) lepB 1068018..1068530 (+) 513 WP_007499139.1 signal peptidase I -
  NST24_RS05360 (NST24_05360) - 1068770..1069912 (-) 1143 WP_007499137.1 AbrB family transcriptional regulator -
  NST24_RS05365 (NST24_05365) - 1070200..1070622 (+) 423 WP_007499135.1 BlaI/MecI/CopY family transcriptional regulator -
  NST24_RS05370 (NST24_05370) - 1070615..1071454 (+) 840 WP_342462420.1 M56 family metallopeptidase -
  NST24_RS05375 (NST24_05375) - 1071513..1071923 (+) 411 WP_008348626.1 DoxX family protein -
  NST24_RS05380 (NST24_05380) - 1072115..1072561 (-) 447 WP_007499129.1 ferritin-like domain-containing protein -
  NST24_RS05385 (NST24_05385) addB 1072728..1076237 (+) 3510 WP_342462421.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  NST24_RS05390 (NST24_05390) addA 1076215..1079919 (+) 3705 WP_138278367.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  NST24_RS05395 (NST24_05395) sbcD 1079945..1081120 (+) 1176 WP_138278368.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1169 a.a.        Molecular weight: 134601.67 Da        Isoelectric Point: 5.2365

>NTDB_id=983012 NST24_RS05385 WP_342462421.1 1072728..1076237(+) (addB) [Bacillus sp. FSL R5-0286]
MEIQFLAGRSGSGKTTAILEEIKEQLRLDPLGPPIIFLVPDQMTFLMEYELAKTSEAGGMIRAKVFSFTRLAWSILQQTG
GANRQFVTSTGIQMLLRKVIEEQKDKFKVFKKASDKPGFVEQIEKTMAEFKRYCMMPEDIEKISASSMHSEYTEERRAAE
KLHDLHVLYQQMEQHLQDEYVHSEDYLTLLAQQIPLSEELRGAHIFIDGFYQFTPQQLLVIEQLLLHAEKITAAFTVDRS
YHDTQPNELDLFRMTGKTYSQLYQLAKECGAAISENILERNKRHLHTPDLAYLENQYEQRPLQPYQEETPHLTVSKSANK
RAEIEGVARDILDLVRGEGLRLRDISIVARHVDDYKDTLKEVFRDYDIPFFIDGNESMQYHPLIEFIRSSLDVIKGNWRY
EAVFRCVKTEFLFPLELAKNKAREQADQLENYCIAYGVKGERWTNGSRFHYRRFQSLDEDFGQTDQEIEMEQMLNDVKEW
IVPPLFQLQKRLKKAQKLRDMVEAVYVFLEEAQVPEKLEKARLEAEEAGRLAEAMQHGQVWDAVIQLMDEFVEMLGEEEL
SFPLFQQMMDTGLSSLKFALIPPSLDQVFIGSMDLSRMYQVKCTFLIGVNDGVIPARPSDESVLSEDDREWLKRAGAELA
ETGKERLLDEQFLIYQALSSPSHYLYLSYSASDTEGRSLLPSTLIKYCEELMPNHQQALYVLDPEQLDDQEQLKFVTNEH
VSLSYTVSQLQQWLNQYPISGVWWSVYNHLMTSSNCDVSKKIISSLFFTNRAKRLKPNVTKELYGDHIQGSVSRMEKFNA
CAFSHFASHGLKLKDRQFYKLEAPDIGQLFHSALKLISDTLVEQKKDWKNLTKEDCVTYSRHAIEQLAPRLQKEILLSSN
RHAYIKEKLQKILIRVSSILSEHAKVSGFSPVGLELGFGGQGPLPPFTFQLKNGCTMELVGRIDRVDKAEGSKGLLLRIV
DYKSSEKGLDLAEVYYGLALQMLTYLDLTITYSKEWLGMEATPAGILYFHIHDPLIQAPIPLGEDAIEQEILKKFKMKGL
LLEDVEAVKLMDQTLESGRSQVIQAGLKKDGSFRSDSAVLSEDHFHILTKHVRRTFEEAGERITSGEVEINPYKLKDQTP
CRFCSFKSICQFDESIEDNEFRVLISEKDDVMLDRIKKEGDQYANTKTE

Nucleotide


Download         Length: 3510 bp        

>NTDB_id=983012 NST24_RS05385 WP_342462421.1 1072728..1076237(+) (addB) [Bacillus sp. FSL R5-0286]
GTGGAGATTCAGTTTTTAGCAGGCAGATCGGGGAGTGGAAAAACGACTGCAATTCTAGAAGAAATCAAAGAACAACTGCG
GCTTGATCCGCTGGGTCCGCCAATCATTTTTTTAGTCCCGGATCAAATGACATTTTTAATGGAATATGAGCTTGCAAAAA
CGTCTGAAGCTGGTGGAATGATCAGGGCCAAAGTCTTTAGTTTTACTCGGCTTGCCTGGTCTATATTACAGCAGACTGGC
GGTGCGAACCGTCAATTTGTCACAAGCACAGGGATTCAAATGCTCTTAAGAAAAGTAATAGAAGAACAGAAAGACAAGTT
TAAGGTATTCAAAAAGGCGAGTGATAAACCGGGATTTGTTGAGCAAATAGAAAAGACGATGGCTGAATTCAAAAGGTACT
GTATGATGCCGGAGGATATTGAAAAAATCTCGGCATCCAGTATGCATTCAGAGTATACAGAAGAAAGACGTGCGGCGGAA
AAATTGCATGATCTTCATGTTCTTTATCAGCAGATGGAGCAGCATCTGCAAGATGAGTATGTGCACTCAGAGGATTACTT
GACACTTCTGGCTCAGCAAATTCCTTTATCAGAAGAATTGAGAGGGGCACACATTTTTATAGATGGTTTTTATCAGTTTA
CCCCCCAGCAGCTTCTTGTCATTGAACAGCTGTTGCTACATGCCGAAAAAATAACAGCTGCATTCACAGTGGATCGTTCC
TATCATGACACGCAGCCAAATGAACTTGATTTATTTCGCATGACAGGTAAGACATATTCCCAGCTTTATCAGTTGGCCAA
AGAGTGCGGAGCGGCTATTTCTGAGAACATTTTGGAGAGAAATAAGCGGCATCTTCATACTCCAGATCTAGCTTATTTAG
AAAATCAATATGAACAGAGACCTTTGCAGCCGTATCAAGAAGAAACGCCTCACCTTACCGTATCGAAAAGTGCAAATAAA
CGAGCTGAAATTGAGGGAGTAGCGAGAGATATTCTTGATTTAGTAAGAGGTGAAGGTCTTAGGTTACGAGACATCTCAAT
TGTTGCAAGACATGTAGACGACTATAAAGATACGTTAAAAGAGGTGTTTCGAGATTACGATATTCCTTTCTTTATTGATG
GAAATGAATCGATGCAGTATCATCCGCTCATTGAGTTCATTCGTTCGAGCCTCGATGTCATAAAAGGGAACTGGCGGTAT
GAGGCGGTGTTTCGCTGTGTGAAAACGGAGTTCTTATTTCCGCTTGAACTCGCCAAAAACAAAGCGAGAGAACAGGCGGA
TCAGCTTGAAAACTATTGTATTGCTTACGGTGTAAAAGGTGAACGCTGGACGAATGGTTCACGCTTTCATTATCGCCGCT
TCCAATCACTAGATGAAGATTTTGGACAAACCGATCAAGAAATAGAAATGGAGCAAATGCTGAATGATGTAAAAGAATGG
ATTGTTCCGCCTCTCTTTCAATTGCAAAAAAGATTGAAAAAAGCGCAAAAACTAAGAGACATGGTGGAGGCAGTGTATGT
CTTTCTAGAAGAAGCCCAAGTGCCAGAAAAGCTTGAAAAGGCAAGGCTTGAGGCAGAAGAAGCAGGCAGGTTAGCTGAAG
CGATGCAGCATGGGCAAGTATGGGATGCTGTCATTCAATTAATGGATGAATTTGTTGAAATGCTGGGCGAGGAGGAGCTC
TCATTTCCATTATTTCAACAGATGATGGATACAGGACTGTCCTCTCTCAAGTTCGCTTTGATTCCCCCTTCACTTGATCA
AGTCTTTATTGGAAGTATGGATTTATCCAGAATGTATCAAGTAAAGTGTACGTTTCTCATTGGAGTAAATGATGGGGTTA
TACCTGCGCGGCCCTCTGATGAAAGTGTATTGTCTGAGGACGACCGGGAATGGTTAAAACGTGCAGGAGCAGAGCTGGCA
GAGACAGGGAAGGAACGGCTGCTGGATGAACAATTTTTGATTTATCAAGCGCTGTCCAGTCCATCGCACTACCTGTATTT
ATCCTATTCAGCTTCAGATACGGAAGGGCGGTCTCTTTTGCCTTCGACCCTCATCAAGTATTGTGAGGAACTGATGCCAA
ACCATCAGCAAGCCCTCTATGTGTTAGATCCAGAGCAGTTGGATGATCAGGAGCAATTAAAATTTGTAACAAACGAGCAT
GTCTCGTTATCCTATACCGTTTCGCAGCTGCAACAATGGCTCAATCAATATCCGATCAGCGGCGTATGGTGGAGTGTATA
TAATCATTTGATGACCTCGTCCAACTGCGACGTATCAAAAAAAATCATATCCAGCTTGTTCTTTACAAATAGAGCGAAAC
GTTTAAAGCCGAATGTCACAAAAGAACTTTACGGTGACCATATTCAGGGCAGTGTTTCAAGAATGGAGAAATTCAATGCA
TGTGCATTTTCTCATTTTGCTTCCCACGGGCTAAAACTAAAAGATCGACAATTTTACAAACTAGAAGCACCTGATATCGG
CCAGCTCTTTCACTCGGCTTTAAAACTGATTTCAGATACGCTTGTTGAACAAAAAAAGGATTGGAAAAACTTAACGAAGG
AAGATTGTGTGACCTACTCAAGGCATGCAATTGAACAGCTGGCTCCACGTCTACAAAAAGAAATTTTATTAAGCTCTAAT
CGGCATGCTTATATAAAAGAAAAGCTTCAAAAAATCCTCATCAGGGTGTCTTCTATATTAAGCGAACATGCAAAAGTGAG
CGGATTCTCTCCGGTTGGACTTGAGTTAGGTTTTGGAGGTCAAGGACCTCTGCCGCCGTTTACTTTTCAATTAAAAAACG
GCTGCACGATGGAGCTTGTTGGAAGGATTGACCGAGTGGATAAAGCAGAAGGATCAAAAGGACTTCTATTAAGAATTGTC
GACTATAAGTCCAGTGAGAAGGGGCTTGATCTAGCAGAAGTTTACTACGGTTTAGCCTTGCAAATGCTCACTTATTTAGA
CCTGACCATTACGTATTCTAAAGAATGGCTTGGCATGGAAGCGACACCGGCAGGAATTTTATACTTCCATATTCATGACC
CGCTGATTCAAGCGCCTATTCCGCTTGGAGAAGATGCAATTGAGCAGGAAATATTGAAGAAATTTAAAATGAAGGGTCTA
TTGCTCGAAGATGTGGAAGCAGTGAAGTTGATGGATCAAACACTCGAATCAGGAAGATCACAAGTCATCCAAGCTGGTTT
GAAAAAGGATGGTTCTTTCCGATCTGACTCTGCGGTATTAAGTGAAGATCATTTTCACATTCTCACAAAACACGTTCGGC
GAACCTTTGAAGAGGCAGGAGAAAGAATTACAAGCGGTGAGGTAGAGATAAATCCATATAAATTAAAGGATCAAACCCCT
TGCCGCTTTTGCTCCTTTAAGTCTATTTGCCAATTTGATGAATCAATAGAAGATAATGAATTTAGGGTGCTCATCTCTGA
GAAGGATGATGTCATGTTAGACAGGATCAAAAAAGAAGGGGATCAGTATGCAAATACCAAAACCGAATAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

66.408

99.316

0.66

Multiple sequence alignment