Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   MHI30_RS14765 Genome accession   NZ_CP151961
Coordinates   2896823..2899171 (-) Length   782 a.a.
NCBI ID   WP_075752053.1    Uniprot ID   -
Organism   Bacillus sp. FSL L8-0528     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2891823..2904171
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHI30_RS14745 (MHI30_14745) - 2891864..2893435 (+) 1572 WP_048349915.1 SH3 domain-containing protein -
  MHI30_RS14750 (MHI30_14750) dtd 2893465..2893908 (-) 444 WP_048349914.1 D-aminoacyl-tRNA deacylase -
  MHI30_RS14755 (MHI30_14755) - 2893922..2896123 (-) 2202 WP_023855390.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
  MHI30_RS14760 (MHI30_14760) - 2896293..2896805 (-) 513 WP_020452411.1 adenine phosphoribosyltransferase -
  MHI30_RS14765 (MHI30_14765) recJ 2896823..2899171 (-) 2349 WP_075752053.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  MHI30_RS14770 (MHI30_14770) - 2899243..2899536 (-) 294 WP_017474367.1 lipopolysaccharide assembly protein LapA domain-containing protein -
  MHI30_RS14775 (MHI30_14775) secDF 2899690..2901915 (-) 2226 WP_003183969.1 protein translocase subunit SecDF -
  MHI30_RS14780 (MHI30_14780) - 2901952..2902248 (-) 297 WP_075752052.1 post-transcriptional regulator -
  MHI30_RS14785 (MHI30_14785) spoVB 2902366..2903919 (+) 1554 WP_075752051.1 stage V sporulation protein B -

Sequence

Protein


Download         Length: 782 a.a.        Molecular weight: 87662.34 Da        Isoelectric Point: 4.9150

>NTDB_id=982272 MHI30_RS14765 WP_075752053.1 2896823..2899171(-) (recJ) [Bacillus sp. FSL L8-0528]
MLASKMRWDVQTPDKEKVEQLAGRLGITPLAASLLVMRGYDTADKASSFLYGNDARFHDPYLLKDMEKAVRRIKQAISDQ
EKIMIYGDYDADGVTSTTVLLQMLKKCSAQADFYIPDRFKEGYGPNEAAFRGIKESGYSLIITVDTGIAGVHEAEVAKEL
GIDLIITDHHEPGPVLPDAYAIIHPKQPGCGYPFKELAGVGVAFKLAHALNGELPEDLLDLCAIGTIADLVPLHDENRQI
AKRGMEQFRKTKRPGLKALAKIAGAEIHNADEETIGFQIAPRLNAVGRIEQADPAVYLLNTEDEREAEELAEDINALNKE
RQKIVNQMTEEAVQMIEEEGLDQPVIVVARPGWNPGVVGIVASKLVERFYRPAIVLGVDEEKGIAKGSARSIKGFDLFQN
LSECRDILPHFGGHPMAAGMTLNLEDVSALRERINRQAEAVLTEADFIPVQDVDLCCRLEDISVDSIAEMNLLAPFGTGN
PKPFVLVSDVKIDDAKKIGANKNHLKLVLADDGNKLDCIGFYKGAIEDGIVPGAKISIVGELSINEWNNRKKPQLMIKDA
AVNEWQLFDLRGKRSWEEKILQLDPAKRACICFREETKAKLAGTGLEEELTVISGPEEAERLDLESRYAVFIDIPPSLDI
FDQVIKGKKLERIYLIFYQSDEHFFSTFPTREYFKWYYAFLLKRGSFDLPKYGGELARHKGWTKDTIDFMTKVFFELGFV
TIENGVLSVVYHAERRDLTDSETYREKQRLIELEQKLIYSSADELKQWLDKRMAENAVAYKV

Nucleotide


Download         Length: 2349 bp        

>NTDB_id=982272 MHI30_RS14765 WP_075752053.1 2896823..2899171(-) (recJ) [Bacillus sp. FSL L8-0528]
ATGTTAGCGTCAAAAATGCGTTGGGACGTGCAAACGCCCGACAAAGAAAAGGTGGAGCAGCTCGCCGGAAGGTTAGGCAT
TACTCCCCTTGCCGCCTCATTGCTTGTCATGAGAGGTTACGATACAGCCGACAAAGCATCTTCATTTTTATATGGCAATG
ATGCACGTTTTCACGATCCTTATCTGCTGAAAGATATGGAAAAAGCTGTACGGCGAATCAAACAGGCAATTTCAGATCAA
GAAAAGATTATGATTTACGGCGATTATGATGCGGACGGTGTGACAAGCACGACTGTCCTTTTGCAAATGTTGAAAAAATG
TTCAGCACAAGCCGATTTTTATATACCTGACCGCTTTAAAGAAGGGTACGGTCCGAATGAAGCGGCCTTTCGCGGGATCA
AAGAATCAGGCTATTCATTGATCATTACAGTTGACACAGGCATTGCCGGCGTTCATGAAGCTGAAGTCGCTAAAGAGCTC
GGCATCGACCTGATCATTACGGATCACCATGAGCCGGGTCCCGTTTTGCCGGATGCTTATGCGATCATTCATCCGAAACA
GCCGGGCTGCGGCTATCCATTTAAAGAGCTTGCCGGCGTCGGTGTGGCATTTAAGCTTGCCCATGCCTTGAACGGAGAGC
TTCCGGAAGATTTGCTCGACCTTTGTGCGATCGGTACGATTGCCGATCTTGTGCCGCTTCATGATGAAAACAGGCAGATA
GCCAAGCGCGGCATGGAACAGTTCAGGAAAACGAAGCGTCCAGGATTAAAAGCGCTGGCGAAAATTGCCGGAGCGGAGAT
ACATAACGCGGATGAAGAGACCATCGGTTTTCAAATCGCGCCCCGCTTAAACGCGGTCGGCAGAATTGAACAGGCTGACC
CGGCCGTTTACCTCCTGAACACAGAAGACGAAAGAGAAGCGGAAGAGCTTGCTGAGGACATCAACGCCTTGAACAAGGAG
CGCCAAAAAATCGTCAATCAGATGACAGAGGAAGCCGTTCAGATGATCGAGGAGGAAGGCCTTGACCAGCCTGTGATCGT
CGTAGCGCGGCCGGGCTGGAATCCTGGCGTCGTGGGAATCGTGGCTTCAAAGCTTGTCGAGCGGTTTTACAGGCCCGCCA
TCGTCCTTGGCGTCGATGAAGAAAAAGGAATTGCCAAAGGATCTGCGCGCAGCATCAAAGGATTTGATTTATTTCAAAAC
CTTTCCGAGTGCCGGGATATTCTCCCGCACTTCGGCGGCCACCCGATGGCAGCCGGAATGACGCTCAACCTGGAAGACGT
TTCGGCTCTGAGGGAGAGAATCAACCGTCAGGCTGAAGCGGTTCTGACCGAGGCAGATTTTATCCCCGTCCAAGACGTCG
ATTTGTGCTGCCGGCTCGAAGATATATCGGTTGACAGCATTGCCGAAATGAACTTGCTGGCGCCGTTCGGCACGGGAAAC
CCGAAGCCGTTCGTTCTTGTCAGCGACGTAAAAATTGATGACGCCAAAAAAATCGGCGCCAACAAGAATCATTTGAAGCT
TGTGCTTGCAGACGACGGGAACAAGCTCGATTGCATCGGATTTTATAAAGGCGCCATCGAAGACGGGATCGTTCCTGGAG
CAAAGATTTCGATTGTCGGGGAACTGTCGATTAACGAGTGGAACAACAGAAAAAAACCGCAGCTGATGATCAAAGATGCG
GCCGTCAATGAATGGCAGCTGTTTGATCTGCGGGGAAAACGGTCATGGGAAGAAAAAATATTGCAGCTGGACCCTGCAAA
ACGGGCGTGCATCTGCTTCCGCGAGGAAACAAAAGCGAAGCTTGCGGGGACAGGCCTTGAAGAAGAGCTGACCGTCATCA
GCGGCCCTGAAGAGGCAGAGCGTCTCGACCTTGAGAGCCGCTATGCGGTATTTATAGATATTCCGCCGTCGCTCGACATC
TTTGACCAAGTGATAAAAGGCAAAAAACTGGAGCGCATTTATCTGATTTTTTATCAATCAGATGAACATTTTTTCTCAAC
ATTTCCAACAAGGGAATACTTTAAATGGTACTATGCTTTCCTCCTGAAAAGAGGTTCTTTTGACTTGCCGAAATACGGCG
GGGAGCTTGCCAGACATAAAGGCTGGACAAAAGACACAATTGATTTCATGACAAAGGTGTTTTTTGAGCTCGGTTTTGTT
ACAATAGAGAATGGTGTTTTATCTGTTGTTTATCATGCCGAAAGGCGTGATCTGACAGATTCTGAAACCTACCGGGAAAA
ACAGCGCCTGATTGAATTGGAGCAAAAGCTCATTTATTCTTCGGCGGATGAGCTTAAACAGTGGCTGGATAAGCGGATGG
CGGAAAACGCCGTCGCTTATAAAGTATGA

Domains

Predicted by InterproScan.

RecJ OB domain (RecJ_OB)

(453-559)

DHH family (DHH)

(82-226)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(566-769)

DHHA1 domain (DHHA1)

(315-436)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

68.374

99.872

0.683

Multiple sequence alignment