Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   MKY25_RS05560 Genome accession   NZ_CP151952
Coordinates   1122700..1125066 (+) Length   788 a.a.
NCBI ID   WP_342443887.1    Uniprot ID   -
Organism   Geobacillus sp. FSL W8-0032     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1117700..1130066
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKY25_RS05545 (MKY25_05545) - 1118749..1119033 (+) 285 WP_033018077.1 post-transcriptional regulator -
  MKY25_RS05550 (MKY25_05550) secDF 1119200..1121452 (+) 2253 WP_342443875.1 protein translocase subunit SecDF -
  MKY25_RS05555 (MKY25_05555) - 1121740..1122642 (+) 903 WP_033018099.1 cation diffusion facilitator family transporter -
  MKY25_RS05560 (MKY25_05560) recJ 1122700..1125066 (+) 2367 WP_342443887.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  MKY25_RS05565 (MKY25_05565) - 1125057..1125569 (+) 513 WP_061912340.1 adenine phosphoribosyltransferase -
  MKY25_RS05570 (MKY25_05570) - 1125840..1128038 (+) 2199 WP_061912339.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
  MKY25_RS05575 (MKY25_05575) dtd 1128063..1128521 (+) 459 WP_061912338.1 D-aminoacyl-tRNA deacylase -
  MKY25_RS05580 (MKY25_05580) - 1128604..1128777 (+) 174 WP_172418467.1 hypothetical protein -
  MKY25_RS05585 (MKY25_05585) ahpC 1128945..1129508 (+) 564 WP_033018069.1 alkyl hydroperoxide reductase subunit C -

Sequence

Protein


Download         Length: 788 a.a.        Molecular weight: 87491.25 Da        Isoelectric Point: 6.2850

>NTDB_id=981905 MKY25_RS05560 WP_342443887.1 1122700..1125066(+) (recJ) [Geobacillus sp. FSL W8-0032]
MLQAKTRWEVERPDEQAVRRLAEEAGIEPLLARLLVRRGVRTAAEAEAFLHPVGQPFHDPFLLHGMERAVRRLERAIHHG
ERVLVYGDYDADGVCSTSVMVSALQEAGAAVEFYIPNRFTEGYGPNLAAFRAAKERGVSVIVTVDNGIAALKEVAAAREW
GLDVIVTDHHEPGPSLPEAYAIIHPKHPGSTYPFHSLAGVGVAFKVAHALLGRVPRHLLDLVAIGTIADLVPLVGENRLL
AAQGLAVLRETTRIGLRALFQQCRIQPSQIDEQTVGFAIAPRLNAVGRLGGADPAVALLMTDDEDEAMQLAAEMDERNRE
RQQLVAQIADEAADIVRRQYPPEQHRVLVVAGEGWNPGVVGIVASKLVEQFYRPAVVLSIDRNKGIAKGSARSIHGFDLF
ASLSKCRDMLPHFGGHPMAAGMTLALDDVERLRQRLNAIAAETLVEDDFIPPTFIDAVCSVEELTLSAARSFERLAPFGV
GNPRPLLLIEEAAVETMRRVGANGAHMKAVFSQQQAAIDVIGFGLGPLCEEIAPGTCVSAVGELLVNEWNGMAKPQLSLC
DVSVNRWQLFDARGCRDVHSLLERLPEEKRLLVAFRRQTETRPDLAPFCRELHFVMTEKEAEALPIDGRYVVLLDLPPRL
DLLGALLGNGQPARIYAVFAQPEGQFFRTFPTRDHFKWFYAFLHKHRSFPLAERGGELARARGWTTETVHFMAEVFLELG
FVREQNGVITLVRQPVKRDLTESRAYRRHRDELEAEHQLLYSTYEQLKRCLTEMIRSQTNEEASASWI

Nucleotide


Download         Length: 2367 bp        

>NTDB_id=981905 MKY25_RS05560 WP_342443887.1 1122700..1125066(+) (recJ) [Geobacillus sp. FSL W8-0032]
ATGTTACAAGCGAAAACGCGTTGGGAGGTGGAGCGTCCCGACGAGCAGGCGGTGAGGCGCCTGGCGGAGGAAGCCGGCAT
CGAACCGCTGCTGGCGCGGCTTCTCGTTCGTCGCGGCGTGCGGACAGCAGCGGAAGCGGAGGCGTTTCTCCATCCCGTTG
GACAGCCGTTTCACGATCCGTTCTTGCTCCATGGGATGGAGCGCGCCGTTCGCCGCCTAGAAAGAGCGATTCACCATGGT
GAACGCGTGCTCGTCTATGGCGACTATGATGCGGATGGTGTTTGCAGCACATCGGTGATGGTGAGCGCTCTTCAAGAGGC
AGGAGCAGCGGTGGAATTTTACATTCCCAACCGCTTTACCGAAGGATACGGCCCGAACTTGGCGGCGTTTCGCGCGGCAA
AGGAGCGCGGCGTGTCCGTCATTGTGACAGTTGACAACGGAATTGCGGCGCTGAAGGAAGTGGCCGCTGCCCGCGAGTGG
GGGCTTGATGTGATCGTGACCGATCATCACGAGCCAGGGCCCTCGCTTCCGGAAGCGTATGCGATCATTCACCCGAAGCA
TCCCGGAAGTACATATCCGTTCCATAGTTTGGCCGGCGTCGGCGTCGCGTTCAAGGTAGCCCATGCGCTGCTCGGACGGG
TGCCGCGCCATTTGCTTGATTTGGTGGCCATCGGCACGATTGCTGATTTAGTGCCGCTTGTCGGGGAGAACCGGCTCTTG
GCTGCGCAAGGGCTGGCGGTATTGCGCGAGACAACCCGCATCGGTTTGCGCGCGCTGTTTCAGCAATGCCGCATCCAACC
ATCCCAGATCGATGAACAGACGGTCGGCTTCGCCATCGCGCCGCGTTTGAACGCGGTCGGGCGTCTGGGCGGCGCCGATC
CGGCGGTGGCGCTGTTAATGACCGATGATGAAGACGAGGCCATGCAGCTGGCGGCGGAAATGGACGAGCGGAATCGCGAG
CGCCAGCAGCTCGTCGCGCAAATCGCCGATGAGGCGGCAGACATCGTGCGCCGCCAATATCCGCCGGAGCAGCATCGCGT
TTTGGTCGTGGCTGGCGAAGGCTGGAATCCCGGAGTGGTCGGCATCGTCGCTTCCAAGCTCGTCGAACAGTTTTACCGGC
CGGCGGTGGTGCTCTCCATTGACCGCAACAAAGGCATCGCCAAAGGCTCGGCGCGCAGCATCCACGGATTTGACTTGTTT
GCCAGTCTCTCCAAATGCCGCGATATGTTGCCGCATTTTGGCGGCCACCCGATGGCGGCGGGGATGACCTTGGCGTTGGA
CGACGTCGAGCGGTTGCGGCAGCGGCTGAACGCGATCGCGGCGGAGACGTTGGTCGAGGACGATTTTATCCCGCCGACGT
TCATCGATGCGGTTTGCTCGGTCGAAGAGCTGACGCTGTCGGCCGCCCGCAGTTTCGAGCGGCTCGCCCCATTCGGTGTC
GGCAATCCGCGACCGCTTTTGCTCATTGAAGAAGCCGCGGTGGAAACGATGCGGCGCGTCGGGGCGAACGGCGCCCATAT
GAAGGCAGTCTTTTCGCAACAGCAGGCGGCGATTGATGTCATCGGCTTCGGCCTCGGTCCCTTGTGCGAGGAGATCGCTC
CGGGGACGTGCGTCTCGGCCGTGGGAGAGCTGTTGGTGAACGAGTGGAACGGGATGGCGAAGCCGCAGTTGTCGCTATGC
GACGTGTCGGTCAACCGCTGGCAGCTGTTTGATGCCCGCGGCTGCCGGGACGTTCATTCGCTTCTTGAGCGGCTGCCAGA
GGAGAAGCGGCTGCTGGTGGCCTTTCGGCGCCAGACGGAAACCCGCCCTGACTTGGCGCCGTTTTGCCGGGAGCTTCATT
TCGTCATGACGGAGAAAGAGGCGGAGGCTTTGCCGATTGACGGCCGCTATGTCGTGCTGCTTGATTTGCCACCGCGGCTT
GATTTGTTGGGGGCGCTTTTAGGCAACGGGCAGCCGGCGCGCATTTATGCCGTGTTCGCGCAGCCGGAAGGGCAATTTTT
CCGCACGTTTCCGACGCGCGACCATTTCAAGTGGTTTTATGCCTTTTTGCATAAGCATCGTTCGTTCCCGCTCGCCGAAC
GCGGCGGCGAATTGGCGCGCGCCCGCGGCTGGACGACAGAAACGGTCCATTTTATGGCCGAAGTCTTTTTGGAGCTGGGG
TTTGTGCGCGAGCAAAACGGCGTTATCACGCTCGTTCGCCAGCCTGTAAAGCGCGATCTCACCGAATCCCGGGCGTACCG
TCGGCACCGGGACGAGCTGGAGGCGGAACATCAGTTATTATACTCCACTTACGAGCAGCTGAAACGCTGTTTGACGGAAA
TGATTCGTTCGCAAACGAATGAGGAGGCAAGCGCATCATGGATTTAA

Domains

Predicted by InterproScan.

DHH family (DHH)

(82-226)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(568-771)

DHHA1 domain (DHHA1)

(316-439)

RecJ OB domain (RecJ_OB)

(455-560)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

53.145

98.858

0.525

Multiple sequence alignment