Detailed information NTG0979062

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: AAEO47_RS17145
• Genome accession: NZ_CP151543
• Coordinates: 3723399..3724547 (-)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain HN232
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3718399..3729547

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AAEO47_RS17125 (AAEO47_17125)	-	3719137..3720321 (-)	1185	WP_003110017.1	cystathionine gamma-synthase	-
AAEO47_RS17130 (AAEO47_17130)	-	3720318..3721691 (-)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
AAEO47_RS17135 (AAEO47_17135)	-	3721934..3722338 (-)	405	WP_003084560.1	NINE protein	-
AAEO47_RS17140 (AAEO47_17140)	czcD	3722492..3723391 (+)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
AAEO47_RS17145 (AAEO47_17145)	pilU	3723399..3724547 (-)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
AAEO47_RS17150 (AAEO47_17150)	pilT	3724725..3725759 (-)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
AAEO47_RS17155 (AAEO47_17155)	-	3725974..3726666 (+)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
AAEO47_RS17160 (AAEO47_17160)	proC	3726678..3727499 (+)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
AAEO47_RS17165 (AAEO47_17165)	-	3727510..3728103 (+)	594	WP_003084544.1	YggT family protein	-
AAEO47_RS17170 (AAEO47_17170)	-	3728100..3728396 (+)	297	WP_031628111.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=979062 AAEO47_RS17145 WP_003100959.1 3723399..3724547(-) (pilU) [Pseudomonas aeruginosa strain HN232]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=979062 AAEO47_RS17145 WP_003100959.1 3723399..3724547(-) (pilU) [Pseudomonas aeruginosa strain HN232]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382