Detailed information NTG0977530

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: ACM6AF_RS06035
• Genome accession: NZ_CP181120
• Coordinates: 1228184..1230592 (+)
• Length: 802 a.a.
• NCBI ID: WP_000560841.1
• Uniprot ID: A0A0H2XFV2
• Organism: Staphylococcus aureus strain AG21-0606
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 1223184..1235592

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACM6AF_RS06010 (ACM6AF_06015)	-	1223292..1223693 (+)	402	WP_000354184.1	VOC family protein	-
ACM6AF_RS06015 (ACM6AF_06020)	-	1223956..1225653 (-)	1698	WP_000312763.1	Rqc2 family fibronectin-binding protein	-
ACM6AF_RS06020 (ACM6AF_06025)	gmk	1225928..1226551 (+)	624	WP_000368227.1	guanylate kinase	-
ACM6AF_RS06025 (ACM6AF_06030)	rpoZ	1226551..1226769 (+)	219	WP_000933956.1	DNA-directed RNA polymerase subunit omega	-
ACM6AF_RS06030 (ACM6AF_06035)	coaBC	1226985..1228184 (+)	1200	WP_000722165.1	bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC	-
ACM6AF_RS06035 (ACM6AF_06040)	priA	1228184..1230592 (+)	2409	WP_000560841.1	primosomal protein N'	Machinery gene
ACM6AF_RS06040 (ACM6AF_06045)	-	1231098..1232051 (+)	954	WP_000751704.1	hypothetical protein	-
ACM6AF_RS06045 (ACM6AF_06050)	-	1232214..1232417 (-)	204	WP_001198024.1	TM2 domain-containing protein	-
ACM6AF_RS06050 (ACM6AF_06055)	-	1232716..1233204 (+)	489	WP_000985293.1	peptide deformylase	-
ACM6AF_RS06055 (ACM6AF_06060)	fmt	1233197..1234132 (+)	936	WP_000161299.1	methionyl-tRNA formyltransferase	-
ACM6AF_RS06060 (ACM6AF_06065)	rsmB	1234129..1235436 (+)	1308	WP_000572235.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-

Sequence

Protein

• Length: 802 a.a.
• Molecular weight: 92522.44 Da
• Isoelectric Point: 6.7333

>NTDB_id=977530 ACM6AF_RS06035 WP_000560841.1 1228184..1230592(+) (priA) [Staphylococcus aureus strain AG21-0606]
MIAKVIVDVASKSVDYKFDYIIPEQLESVIQPGVRVIVPFGPRTIQGYVMEVTAEPDAQLDVSKLKKIIEVKDIQPELTS
ELIALSEWMGSTHVIKRISMLEVMLPSAIKAKYKKAFKMKDDIELPSALLQKFDKHGYYYYKDAQKNNDIQLLMKLLKDD
IVEEKTILTQNITKKTKRAVRVIEGYHPDEVLAKLEKVIKQYDLYAYLSEEQHKTIFLTDIEDMGFSKSSLDGLIKKGYV
EKYDAVVERDPFKDRVFEQESKQQLTEDQYKAYEAIKAKIVSQEQETFLLHGVTGSGKTEVYLQTIEDVLSQGKQAMMLV
PEIALTPQMVLRFKRRFGDDVAVLHSGLSNGERYDEWQKIRDGRARVSVGARSSVFAPFKNLGLIIIDEEHESTYKQEDY
PRYHAREIAQWRSEYHHCPVILGSATPCLESYARAEKGVYHLLSLPNRVNQQALPEIDIVDMREELSEGNRSMFSKDLRE
AIQLRLDRQEQVVLFLNRRGYASFMLCRDCGYVPQCPNCDISLTYHKTTDLLKCHYCGYQETPPNQCPNCESEHIRQVGT
GTQKVEELLQQEFEDARIIRMDVDTTSKKGAHEKLLTEFEKGNGDILLGTQMIAKGLDYPNITLVGVLNADTMLNLPDFR
ASERTYQLLTQVAGRAGRHEKAGQVIIQTYNPDHYSILDVQKNDYLTFYRQEMEYRKLGKYPPYYYLINFTISHKEMKKV
MVASQHVHKILLQHLTEKALVLGPSPAALARINNEFRFQILVKYKSEPGLLQAIQFLDDYYHEKFIKEKLALKIDIDPQM
MM

Nucleotide

• Length: 2409 bp

>NTDB_id=977530 ACM6AF_RS06035 WP_000560841.1 1228184..1230592(+) (priA) [Staphylococcus aureus strain AG21-0606]
ATGATAGCGAAAGTCATAGTCGATGTCGCGTCGAAGAGCGTTGACTATAAATTTGATTATATAATTCCCGAACAACTCGA
ATCTGTCATCCAACCTGGTGTGCGTGTAATTGTACCTTTTGGACCAAGAACGATTCAAGGTTATGTAATGGAAGTAACAG
CAGAACCTGATGCACAACTTGACGTTTCGAAGTTAAAAAAAATCATAGAAGTGAAAGATATACAACCAGAATTAACATCA
GAATTAATAGCTTTAAGTGAGTGGATGGGTTCAACTCATGTCATTAAACGTATTTCTATGCTAGAAGTGATGCTTCCGAG
TGCTATTAAAGCGAAGTATAAAAAAGCATTTAAGATGAAAGATGACATAGAGCTACCTTCAGCTTTATTACAAAAATTTG
ATAAGCATGGTTACTATTATTATAAAGATGCGCAAAAAAATAATGATATTCAATTGCTTATGAAGTTGTTAAAAGATGAT
ATCGTTGAAGAAAAAACGATTCTCACACAAAATATAACTAAAAAAACCAAGCGTGCTGTTCGTGTCATTGAAGGGTATCA
TCCTGATGAAGTATTAGCTAAGTTGGAGAAAGTTATTAAACAATACGATTTGTATGCTTACTTGTCTGAAGAACAACATA
AAACAATATTTTTAACTGATATTGAGGATATGGGCTTTTCAAAATCCAGTTTAGATGGACTTATCAAAAAAGGTTATGTT
GAAAAATATGACGCGGTTGTTGAAAGAGACCCATTTAAAGATCGTGTTTTCGAACAAGAATCAAAACAGCAATTAACAGA
AGACCAATATAAAGCATATGAAGCGATTAAAGCTAAAATTGTAAGCCAAGAGCAAGAAACATTTTTACTTCATGGTGTGA
CGGGATCAGGTAAAACAGAAGTATATTTACAAACGATAGAAGATGTTTTAAGCCAAGGAAAACAGGCGATGATGTTAGTT
CCTGAAATCGCTCTAACACCGCAAATGGTTTTACGCTTCAAACGTCGATTTGGTGATGACGTTGCTGTATTACATTCTGG
CTTATCTAATGGGGAACGTTATGATGAATGGCAAAAAATTAGGGATGGTCGTGCGAGAGTAAGTGTTGGTGCAAGGTCAA
GTGTGTTCGCACCTTTCAAAAATTTAGGGTTAATCATCATTGATGAAGAACATGAATCTACATATAAACAAGAAGATTAT
CCGAGATATCACGCTAGAGAAATTGCCCAATGGCGAAGTGAATATCATCACTGTCCAGTCATTTTAGGAAGTGCAACACC
ATGTCTTGAAAGTTATGCACGAGCTGAAAAAGGCGTTTATCATTTGCTATCATTACCAAACAGAGTGAACCAACAAGCTT
TACCTGAAATTGATATAGTAGACATGCGTGAAGAATTGAGTGAAGGTAATCGGTCAATGTTTTCAAAAGATTTACGTGAA
GCCATACAATTAAGATTAGATCGACAGGAACAAGTTGTTTTATTTTTAAATCGACGTGGTTATGCATCGTTTATGTTATG
TCGGGATTGTGGATATGTACCGCAATGTCCAAACTGTGATATTTCATTAACGTATCATAAAACGACAGACTTATTAAAAT
GTCACTATTGTGGTTACCAAGAGACGCCACCGAATCAATGTCCAAATTGTGAGAGTGAACACATTCGACAAGTAGGTACT
GGTACTCAGAAAGTTGAAGAACTATTGCAACAAGAATTTGAAGATGCGCGCATAATTAGGATGGATGTAGATACAACCTC
AAAGAAAGGTGCACATGAAAAGTTATTGACTGAATTCGAAAAAGGTAACGGTGACATTTTACTAGGTACTCAGATGATTG
CGAAAGGATTAGATTATCCAAATATTACTTTAGTTGGTGTGCTGAATGCAGATACAATGTTAAATTTACCTGATTTTCGG
GCGAGCGAACGTACTTATCAACTATTAACGCAAGTGGCTGGTAGAGCTGGTCGTCATGAAAAGGCAGGTCAAGTCATCAT
TCAAACGTATAATCCAGATCATTATTCAATATTGGATGTTCAAAAAAATGATTATTTAACATTTTATCGTCAGGAAATGG
AATATCGTAAATTAGGAAAGTATCCACCGTATTATTATTTGATTAATTTCACAATCTCACATAAAGAAATGAAGAAGGTT
ATGGTAGCATCGCAGCATGTTCATAAAATTTTATTACAGCATTTAACAGAAAAAGCGCTTGTACTAGGTCCATCTCCGGC
AGCACTTGCGAGAATCAACAATGAATTTAGATTCCAAATTTTAGTGAAATATAAAAGTGAACCTGGATTATTACAAGCCA
TTCAGTTTTTAGATGACTATTACCATGAAAAATTTATAAAAGAAAAATTAGCATTGAAGATTGATATTGATCCACAGATG
ATGATGTAA

Domains

Predicted by InterProScan.

• Type III restriction enzyme, res subunit (ResIII) (264-427)

• Helicase conserved C-terminal domain (Helicase_C) (564-659)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (4-106)

• Primosomal protein N C-terminal domain (PriA_C) (703-798)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (516-541)

3D structure

• AlphaFold DB: A0A0H2XFV2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Bacillus subtilis subsp. subtilis str. 168	50.68	100	0.511