Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiE   Type   Regulator
Locus tag   ACL656_RS01195 Genome accession   NZ_CP178597
Coordinates   273677..274441 (-) Length   254 a.a.
NCBI ID   WP_001136201.1    Uniprot ID   -
Organism   Escherichia coli strain ZF8-1     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 268677..279441
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACL656_RS01170 (ACL656_01170) yhhJ 270533..271657 (+) 1125 WP_001314210.1 ABC transporter permease -
  ACL656_RS01175 (ACL656_01175) - 271761..271991 (+) 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin -
  ACL656_RS01180 (ACL656_01180) - 271988..272347 (+) 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin -
  ACL656_RS01185 (ACL656_01185) nikR 272467..272868 (-) 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
  ACL656_RS01190 (ACL656_01190) nikE 272874..273680 (-) 807 WP_001603990.1 nickel import ATP-binding protein NikE -
  ACL656_RS01195 (ACL656_01195) amiE 273677..274441 (-) 765 WP_001136201.1 nickel import ATP-binding protein NikD Regulator
  ACL656_RS01200 (ACL656_01200) nikC 274441..275274 (-) 834 WP_001008970.1 nickel ABC transporter permease subunit NikC -
  ACL656_RS01205 (ACL656_01205) nikB 275271..276215 (-) 945 WP_000947082.1 nickel ABC transporter permease subunit NikB -
  ACL656_RS01210 (ACL656_01210) nikA 276215..277789 (-) 1575 WP_053292180.1 nickel ABC transporter substrate-binding protein -
  ACL656_RS01215 (ACL656_01215) acpT 277900..278487 (-) 588 WP_000285784.1 4'-phosphopantetheinyl transferase AcpT -

Sequence

Protein


Download         Length: 254 a.a.        Molecular weight: 26780.35 Da        Isoelectric Point: 6.2250

>NTDB_id=972468 ACL656_RS01195 WP_001136201.1 273677..274441(-) (amiE) [Escherichia coli strain ZF8-1]
MPQQIELRDIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSLTCAAALGILPAGVRQTAGEILADGKPVSPCALRGIK
IATIMQNPRSAFNPLRTMHTHARETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGKIVEQGDVETLFNAPKHAVTRSL
VSAHLALYGMELAS

Nucleotide


Download         Length: 765 bp        

>NTDB_id=972468 ACL656_RS01195 WP_001136201.1 273677..274441(-) (amiE) [Escherichia coli strain ZF8-1]
ATGCCGCAACAGATTGAACTGCGCGATATCGCGCTACAGGCCGCGCAGCCGCTGGTGCACGGCGTATCGTTAACCCTGCA
ACGCGGGCGCGTGCTGGCGTTAGTCGGCGGTAGCGGCAGCGGGAAATCATTAACCTGCGCCGCGGCGCTGGGAATTCTGC
CCGCAGGCGTTCGCCAGACGGCGGGGGAAATTTTAGCCGATGGCAAACCGGTTTCTCCCTGCGCCCTGCGCGGCATCAAA
ATTGCCACCATCATGCAGAACCCGCGCAGCGCCTTTAATCCGCTACGCACCATGCACACCCACGCGCGTGAAACCTGCCT
GGCGCTGGGGAAACCCGCCGATGACGCTACGCTTACCGCTGCCATAGAAGCGGTGGGGCTGGAAAACGCCGCACGCGTGC
TGAAGCTGTACCCGTTCGAGATGAGCGGCGGCATGTTGCAGCGCATGATGATTGCGATGGCGGTGCTGTGTGAATCACCG
TTTATCATCGCCGATGAACCGACCACTGACCTCGATGTAGTAGCGCAGGCGCGCATCCTCGATCTGCTGGAAAGCATTAT
GCAAAAACAGGCACCGGGGATGCTGCTGGTCACCCATGATATGGGCGTAGTGGCGCGTCTGGCGGATGACGTGGCGGTGA
TGTCTGACGGTAAGATTGTCGAACAGGGCGATGTCGAAACGCTGTTTAACGCCCCCAAACATGCGGTAACGCGCAGCCTG
GTTTCCGCTCATCTCGCCCTCTACGGTATGGAGCTGGCATCATGA

Domains

Predicted by InterProScan.

ABC transporter (ABC_tran)

(20-168)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiE Streptococcus thermophilus LMG 18311

42

98.425

0.413
  amiE Streptococcus thermophilus LMD-9

42

98.425

0.413
  amiE Streptococcus salivarius strain HSISS4

40.8

98.425

0.402

Multiple sequence alignment