Detailed information NTG0971000

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: ACLFAM_RS04195
• Genome accession: NZ_CP178226
• Coordinates: 845717..848056 (-)
• Length: 779 a.a.
• NCBI ID: WP_023189130.1
• Uniprot ID: -
• Organism: Lactococcus lactis strain L-WY410
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 840717..853056

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACLFAM_RS04155 (ACLFAM_04155)	-	841633..842139 (-)	507	WP_012898444.1	Asp23/Gls24 family envelope stress response protein	-
ACLFAM_RS04160 (ACLFAM_04160)	-	842160..842348 (-)	189	WP_012898445.1	DUF2273 domain-containing protein	-
ACLFAM_RS04165 (ACLFAM_04165)	amaP	842359..842919 (-)	561	WP_010906186.1	alkaline shock response membrane anchor protein AmaP	-
ACLFAM_RS04170 (ACLFAM_04170)	-	842947..843189 (-)	243	WP_003131476.1	GlsB/YeaQ/YmgE family stress response membrane protein	-
ACLFAM_RS04175 (ACLFAM_04175)	fmt	843364..844323 (-)	960	WP_023189127.1	methionyl-tRNA formyltransferase	-
ACLFAM_RS04180 (ACLFAM_04180)	-	844320..844784 (-)	465	WP_003131474.1	VOC family protein	-
ACLFAM_RS04185 (ACLFAM_04185)	-	844750..845271 (-)	522	WP_023189128.1	NUDIX hydrolase	-
ACLFAM_RS04190 (ACLFAM_04190)	-	845284..845658 (-)	375	WP_025017158.1	nuclear transport factor 2 family protein	-
ACLFAM_RS04195 (ACLFAM_04195)	priA	845717..848056 (-)	2340	WP_023189130.1	primosomal protein N'	Machinery gene
ACLFAM_RS04200 (ACLFAM_04200)	rpoZ	848195..848548 (-)	354	WP_010906189.1	DNA-directed RNA polymerase subunit omega	-
ACLFAM_RS04205 (ACLFAM_04205)	gmk	848728..849345 (-)	618	WP_003131468.1	guanylate kinase	-
ACLFAM_RS04210 (ACLFAM_04210)	rny	849493..851088 (-)	1596	WP_023189132.1	ribonuclease Y	-
ACLFAM_RS04215 (ACLFAM_04215)	-	851319..852032 (-)	714	WP_039116154.1	glucosamine-6-phosphate deaminase	-

Sequence

Protein

• Length: 779 a.a.
• Molecular weight: 88945.26 Da
• Isoelectric Point: 6.5580

>NTDB_id=971000 ACLFAM_RS04195 WP_023189130.1 845717..848056(-) (priA) [Lactococcus lactis strain L-WY410]
MKIAKVIVDVPLMQTDKPFSYSLSAEFENLLEVGMRVHVPFGSGNRLVQGIVIGFEDEELEPSVNLKGISELLDVEPVLT
DEQLWLADEMRHSVFSYKISCLKAMLPNLLNSNYDKLLKSTDGTSVKWSQLTEAEKISSAKAVRLGQLSVEYLAKSKENR
KTEKFLSIPDIEKLAEFEIEKRAKKRLELKYFLLRLQEEKIAVKSLNFSSQIIKFFVEHDLVKIEEVEISRTKEKFEDVE
NDSAKILNSEQNASYEKIIASSDKPFLLEGITGSGKTEVYLQTIDHFLKLGKTAIMLVPEISLTPQITNRFIARFGDKVA
IMHSGLSDGEKYDEWRKIKSGKAQVVVGARSAIFAPVENLGIIIIDEEHETSYKQDSSPRYHARDIALLRAKWHGARLVL
GSATPSLETRARAMRGVYELLLLTKRANEAAILPEIKIIDMRSEISDQSANFSQSLLTKIKEKIDKDEQVILMLNRRGYS
SFVMCRECGFVVDCPNCDISLTLHMDTRTLNCHYCGHVEGIPQTCPNCHSRKIRYYGSGTQKVEEELREIMPEARVLRMD
VDTTRTKNAHEKILEKFGNHEADILLGTQMIAKGLDFPNVTLVGVINADTGLNLPDFRASERSFELLMQVAGRAGRADKK
GEVLIQTFNPHHYAIEMTKAQDYEKFYEMEMRFRRELSYPPYYFTVQVLVSHQDEEVAIQKSYEIAQLLKQNLTEKAKIL
GPTARPIARTHNLYHYQILVKYRFEEELESALNQVLELTQLRDNKNLRVLIDSEPQNFV

Nucleotide

• Length: 2340 bp

>NTDB_id=971000 ACLFAM_RS04195 WP_023189130.1 845717..848056(-) (priA) [Lactococcus lactis strain L-WY410]
ATGAAAATTGCTAAAGTCATCGTTGATGTGCCTCTGATGCAGACGGATAAACCCTTTTCTTACAGCCTTTCAGCTGAATT
TGAAAATTTGCTTGAAGTGGGCATGCGGGTTCATGTTCCTTTTGGAAGTGGAAACCGGCTTGTTCAAGGAATTGTCATTG
GATTTGAAGATGAAGAACTTGAGCCGTCAGTAAATTTGAAAGGAATTTCTGAACTACTCGACGTTGAGCCAGTCCTAACG
GATGAACAGCTTTGGTTGGCAGATGAAATGCGTCATTCAGTATTTTCTTATAAAATTTCATGTCTAAAAGCCATGTTACC
TAATCTTTTGAATTCTAATTATGACAAGCTCCTAAAATCGACTGACGGAACTTCTGTCAAATGGTCACAACTGACAGAAG
CAGAAAAAATCTCGTCAGCAAAGGCTGTGCGTTTGGGTCAGCTGTCAGTAGAATATTTAGCAAAATCTAAAGAAAATAGA
AAAACAGAAAAATTCTTATCTATTCCAGATATCGAAAAACTAGCTGAATTTGAAATTGAAAAGAGAGCCAAAAAACGCTT
AGAGTTGAAATATTTTCTATTAAGACTTCAAGAAGAAAAAATTGCAGTCAAATCATTAAATTTTTCTTCACAAATCATTA
AATTTTTCGTTGAGCATGACCTAGTCAAAATTGAAGAGGTTGAAATTTCGAGGACGAAAGAAAAATTTGAAGATGTGGAA
AATGACAGTGCTAAAATTCTCAACTCCGAACAAAATGCATCTTACGAAAAAATAATTGCATCTTCTGACAAACCATTTTT
GTTGGAAGGAATTACTGGTTCAGGTAAAACAGAAGTTTACTTACAGACCATTGACCATTTTCTCAAACTCGGTAAAACAG
CAATTATGCTTGTGCCAGAAATTTCTTTGACGCCACAGATTACTAATCGATTTATTGCTCGTTTTGGAGATAAAGTGGCG
ATTATGCATTCTGGTTTATCTGATGGTGAAAAATATGATGAATGGCGAAAAATTAAATCTGGCAAAGCTCAGGTAGTTGT
CGGTGCACGTTCAGCTATTTTTGCACCTGTTGAAAATTTGGGAATTATCATCATTGATGAAGAACATGAAACTTCATATA
AGCAAGATTCAAGTCCCCGTTATCATGCAAGAGATATTGCGCTTTTGCGTGCAAAATGGCATGGTGCAAGACTTGTTTTA
GGTTCTGCAACGCCAAGTTTAGAAACGCGAGCACGTGCCATGCGCGGAGTTTATGAACTTTTATTACTGACAAAAAGGGC
AAATGAAGCAGCAATCCTGCCAGAAATAAAAATCATTGATATGCGCAGTGAAATTTCTGACCAATCTGCTAATTTTTCAC
AATCTTTACTGACAAAAATAAAAGAAAAAATTGACAAAGATGAACAAGTCATTTTGATGTTGAATCGAAGGGGTTATTCT
TCATTTGTCATGTGTCGTGAATGTGGTTTTGTTGTTGATTGTCCCAATTGCGATATTTCCTTGACCCTTCACATGGATAC
CCGAACTTTGAATTGTCATTATTGTGGTCATGTCGAAGGAATTCCGCAAACTTGTCCTAATTGTCATTCACGAAAAATTC
GTTATTACGGTTCAGGAACGCAAAAAGTCGAAGAAGAACTTCGAGAAATCATGCCTGAAGCACGAGTTTTGCGAATGGAT
GTAGATACGACTCGGACAAAAAATGCTCACGAAAAAATTCTTGAAAAGTTTGGTAATCATGAAGCTGATATCCTCTTAGG
AACTCAGATGATTGCCAAAGGTTTAGATTTTCCAAACGTAACCTTAGTGGGTGTCATTAACGCAGATACTGGCCTAAATC
TACCTGATTTTAGAGCGAGTGAGCGTTCCTTTGAATTGTTGATGCAGGTGGCTGGACGAGCTGGACGAGCAGATAAAAAA
GGTGAAGTATTAATTCAAACTTTCAATCCACATCATTATGCTATTGAAATGACCAAGGCTCAAGATTACGAAAAATTTTA
TGAAATGGAAATGAGATTTCGTCGTGAACTTTCCTATCCACCTTATTATTTCACCGTTCAAGTGCTTGTTTCTCATCAAG
ATGAAGAAGTTGCCATTCAAAAATCTTATGAGATCGCTCAATTACTCAAGCAAAATTTGACAGAAAAAGCTAAAATATTA
GGGCCAACCGCCAGACCAATTGCCAGAACACATAACCTTTATCATTATCAAATCTTAGTTAAATATCGTTTTGAAGAAGA
ATTAGAATCAGCTTTAAATCAAGTGCTTGAATTAACCCAACTTCGGGATAACAAAAATTTACGAGTATTGATTGATAGTG
AGCCACAAAATTTTGTATAA

Domains

Predicted by InterProScan.

• 3DNA-binding domain (3BD) (PriA_3primeBD) (5-107)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (494-518)

• Helicase conserved C-terminal domain (Helicase_C) (561-637)

• Type III restriction enzyme, res subunit (ResIII) (245-405)

• Primosomal protein N C-terminal domain (PriA_C) (681-775)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Bacillus subtilis subsp. subtilis str. 168	47.388	100	0.489