Detailed information NTG0970994

Bioinformatically predicted

Overview

• Name: endA
• Type: Machinery gene
• Locus tag: ACLFAM_RS03435
• Genome accession: NZ_CP178226
• Coordinates: 720252..721031 (-)
• Length: 259 a.a.
• NCBI ID: WP_050428201.1
• Uniprot ID: -
• Organism: Lactococcus lactis strain L-WY410
• Function: cleavage of dsDNA into ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 715252..726031

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACLFAM_RS03405 (ACLFAM_03405)	-	716045..716446 (-)	402	WP_185280440.1	hypothetical protein	-
ACLFAM_RS03410 (ACLFAM_03410)	-	716443..716643 (-)	201	WP_039115873.1	DUF1655 domain-containing protein	-
ACLFAM_RS03415 (ACLFAM_03415)	-	716991..717167 (-)	177	WP_039115875.1	helix-turn-helix domain-containing protein	-
ACLFAM_RS03420 (ACLFAM_03420)	-	717312..718286 (+)	975	WP_039115877.1	helix-turn-helix domain-containing protein	-
ACLFAM_RS03425 (ACLFAM_03425)	-	718366..719565 (+)	1200	WP_039115879.1	tyrosine-type recombinase/integrase	-
ACLFAM_RS03430 (ACLFAM_03430)	-	719712..720161 (+)	450	WP_025017176.1	GNAT family N-acetyltransferase	-
ACLFAM_RS03435 (ACLFAM_03435)	endA	720252..721031 (-)	780	WP_050428201.1	DNA/RNA non-specific endonuclease	Machinery gene
ACLFAM_RS03440 (ACLFAM_03440)	-	721225..722121 (-)	897	WP_039115881.1	peptidoglycan amidohydrolase family protein	-
ACLFAM_RS03445 (ACLFAM_03445)	-	722233..722487 (-)	255	WP_023189062.1	phage holin	-
ACLFAM_RS03450 (ACLFAM_03450)	-	722477..722779 (-)	303	WP_025017161.1	hypothetical protein	-
ACLFAM_RS03455 (ACLFAM_03455)	-	722984..723277 (+)	294	WP_025017162.1	hypothetical protein	-
ACLFAM_RS03460 (ACLFAM_03460)	-	723307..725547 (-)	2241	WP_052230930.1	glycoside hydrolase domain-containing protein	-
ACLFAM_RS03465 (ACLFAM_03465)	-	725647..725994 (-)	348	WP_023189066.1	DUF722 domain-containing protein	-

Sequence

Protein

• Length: 259 a.a.
• Molecular weight: 29129.05 Da
• Isoelectric Point: 9.0583

>NTDB_id=970994 ACLFAM_RS03435 WP_050428201.1 720252..721031(-) (endA) [Lactococcus lactis strain L-WY410]
MKNKKIIPLLIVSIFILGGCANSQSDMDKSNHKWSQKDKTPSEELAKSVLTSSIKEQLKTDNIKFDHGSFIVNNNNNDLA
IINVVDPYAKNEIDIQGRPYLGEAVLPKSIQNNEKDNKDEKVIPQFGWGQKSNLKGFYKKAYTKGFLLNSKLIGGVKDFN
DSGSNMENIFTQTVWASEANDSSSKGQKYFENMIHKVQDKNMIIKYRVKALYDGSNLVPSGTEIEAKSDDFSLEIHVFIP
NVQPNLKVDYKTGQVTEVK

Nucleotide

• Length: 780 bp

>NTDB_id=970994 ACLFAM_RS03435 WP_050428201.1 720252..721031(-) (endA) [Lactococcus lactis strain L-WY410]
ATGAAGAACAAAAAAATTATCCCATTACTGATTGTCAGCATTTTTATCTTAGGGGGCTGTGCTAACAGTCAATCTGATAT
GGATAAAAGTAATCATAAATGGAGTCAAAAAGATAAGACACCCTCAGAAGAACTCGCAAAAAGTGTTCTGACTTCTTCAA
TAAAAGAACAATTAAAAACAGATAACATAAAATTTGATCACGGTTCATTTATCGTTAATAATAATAACAATGATTTAGCA
ATTATTAATGTTGTAGACCCATACGCTAAGAATGAAATAGATATTCAAGGGCGTCCTTATTTGGGTGAAGCAGTTCTCCC
AAAATCTATTCAAAATAATGAAAAGGATAATAAAGATGAAAAAGTGATTCCTCAATTTGGTTGGGGACAAAAATCTAACT
TGAAAGGCTTCTATAAAAAAGCTTATACTAAAGGTTTTTTGCTAAATAGCAAACTAATAGGTGGCGTTAAAGATTTCAAT
GATAGCGGGTCAAATATGGAAAATATATTTACTCAAACGGTTTGGGCGAGTGAAGCTAATGATTCAAGTTCCAAGGGACA
AAAATATTTTGAAAATATGATTCATAAAGTTCAAGATAAGAATATGATCATCAAGTATAGAGTGAAAGCACTCTATGATG
GAAGTAACCTTGTCCCATCAGGAACTGAAATAGAAGCAAAATCTGATGATTTTTCACTCGAGATACATGTTTTTATTCCA
AATGTTCAACCAAATCTTAAAGTAGATTATAAAACAGGTCAGGTCACTGAAGTCAAATAA

Domains

Predicted by InterProScan.

• DNA/RNA non-specific endonuclease (Endonuclea_NS_2) (92-229)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
endA	Streptococcus pneumoniae Rx1	42.174	88.803	0.375
endA	Streptococcus pneumoniae D39	42.174	88.803	0.375
endA	Streptococcus pneumoniae R6	42.174	88.803	0.375
endA	Streptococcus pneumoniae TIGR4	42.174	88.803	0.375