Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   MHI44_RS04370 Genome accession   NZ_CP150268
Coordinates   796835..799195 (-) Length   786 a.a.
NCBI ID   WP_116316480.1    Uniprot ID   -
Organism   Bacillus sp. FSL K6-1366     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 791835..804195
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MHI44_RS04350 (MHI44_04350) - 791909..793465 (+) 1557 WP_029727135.1 SH3 domain-containing protein -
  MHI44_RS04355 (MHI44_04355) dtd 793492..793932 (-) 441 WP_004398689.1 D-aminoacyl-tRNA deacylase -
  MHI44_RS04360 (MHI44_04360) relA 793945..796149 (-) 2205 WP_003229747.1 GTP diphosphokinase -
  MHI44_RS04365 (MHI44_04365) - 796317..796829 (-) 513 WP_033882959.1 adenine phosphoribosyltransferase -
  MHI44_RS04370 (MHI44_04370) recJ 796835..799195 (-) 2361 WP_116316480.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  MHI44_RS04375 (MHI44_04375) - 799262..799585 (-) 324 WP_003229740.1 lipopolysaccharide assembly LapA domain-containing protein -
  MHI44_RS04380 (MHI44_04380) - 799661..800158 (-) 498 WP_339202966.1 cation:proton antiporter regulatory subunit -
  MHI44_RS04385 (MHI44_04385) secDF 800316..802529 (-) 2214 WP_003245970.1 protein translocase subunit SecDF -
  MHI44_RS04390 (MHI44_04390) comN 802568..802864 (-) 297 WP_033882954.1 post-transcriptional regulator ComN -

Sequence

Protein


Download         Length: 786 a.a.        Molecular weight: 88032.70 Da        Isoelectric Point: 5.1353

>NTDB_id=968801 MHI44_RS04370 WP_116316480.1 796835..799195(-) (recJ) [Bacillus sp. FSL K6-1366]
MLASKMRWEIQRPDQDKVKSLTEQLHITPLVASLLVKRGFDTAESARLFLHTKDADFYDPFEMKGMKEAADRIKQAISQQ
EKIMIYGDYDADGVTSTSVMLHTLQKLSAQVDFYIPDRFKEGYGPNEQAFRSIKERGFSLIITVDTGIAAVHEAKVAKEL
GLDVIITDHHEPGPELPDVHAIVHPKQPGCTYPFKELAGVGVAFKLAHALLGELPDELLDLAAIGTIADLVPLHDENRLI
ATLGLERLRRTNRLGLKELIKLSGGDIGEANEETVGFQLAPRLNAVGRIEQADPAVHLLMSEDSFEAEELAAEIDQLNKE
RQKMVSKMTDEAIEMVEQQGLDQTAIVVAKAGWNPGVVGIVASKLVDRFYRPAIVLGIDEEKGIAKGSARSIRGFNLFES
LSECRDILPHFGGHPMAAGMTLKAEDVPDLRSRLNEIADNTLTEEDFIPVQEVDLVCGVEDITVESIAEMNMLSPFGMLN
PKPHVLVENAVLEDVRKIGANKTHVKMTIRNESSQLDCVGFNKGELEEGIVPGSRISIVGEMSINEWNNRKKPQLMIKDA
AVSEWQLFDLRGKRTWEDTVSVLPSAKRAIVSFKEDSTTLLQTEDLRREVHVISSKDQAKAFDLDGAYIVLLDPPPSLDM
LARLLEGKAPERIYFIFLNHEDHFLSTFPARDHFKWYYAFLLKRGAFDVKKHGSELAKHKGWSVETINFMTKVFFDLGFV
KIENGVLSVVSGAKKRDLTDSQTYQAKQQLMELDQKLNYSSAEELKEWLNKLMKQDSEAYESTRRT

Nucleotide


Download         Length: 2361 bp        

>NTDB_id=968801 MHI44_RS04370 WP_116316480.1 796835..799195(-) (recJ) [Bacillus sp. FSL K6-1366]
ATGTTAGCGTCAAAAATGCGATGGGAAATCCAGCGACCAGATCAGGATAAGGTCAAATCACTCACAGAACAATTGCATAT
AACACCTCTTGTTGCGTCCCTGCTTGTAAAAAGAGGCTTTGATACGGCAGAAAGCGCTAGGCTGTTTTTACATACAAAGG
ATGCCGATTTTTATGATCCTTTTGAAATGAAAGGCATGAAAGAAGCGGCAGATCGAATAAAACAGGCGATTTCACAACAA
GAAAAGATTATGATATATGGCGATTATGACGCCGATGGGGTTACAAGTACGTCAGTAATGCTTCACACGCTGCAAAAACT
GTCGGCTCAAGTTGATTTTTATATACCTGACCGTTTTAAAGAAGGATATGGACCTAATGAACAGGCGTTTCGTTCGATTA
AAGAACGGGGCTTCTCTTTGATCATTACGGTTGATACAGGAATTGCCGCTGTGCATGAAGCGAAGGTGGCAAAGGAACTC
GGACTGGATGTCATCATTACCGATCATCATGAACCGGGACCGGAGCTTCCGGATGTCCATGCGATCGTCCACCCGAAGCA
GCCTGGCTGCACTTACCCGTTTAAAGAGCTTGCCGGGGTTGGAGTCGCGTTTAAGCTTGCCCACGCTTTATTGGGTGAAC
TTCCTGATGAACTGCTTGACCTGGCGGCTATCGGCACCATTGCCGATCTAGTCCCGCTGCATGACGAAAACAGATTAATT
GCAACACTAGGACTTGAAAGGCTTCGACGGACAAATCGGCTCGGCTTAAAAGAGCTGATTAAGCTGTCAGGCGGAGACAT
CGGAGAAGCGAACGAAGAAACTGTCGGATTTCAGCTCGCACCAAGGCTGAATGCCGTCGGAAGAATCGAACAGGCAGATC
CCGCTGTTCATTTGCTTATGTCAGAGGATTCGTTTGAAGCGGAAGAGCTTGCCGCAGAAATTGACCAGCTGAACAAAGAG
CGCCAAAAAATGGTCAGCAAAATGACGGATGAAGCGATTGAAATGGTTGAACAGCAAGGGCTCGATCAAACTGCAATTGT
CGTGGCCAAAGCCGGATGGAACCCGGGAGTCGTCGGGATTGTGGCTTCAAAGCTTGTGGACCGTTTCTACCGACCGGCCA
TTGTTCTTGGCATTGATGAAGAAAAGGGAATCGCTAAAGGTTCGGCCCGAAGCATCAGAGGATTTAATTTATTTGAGAGC
CTTTCTGAATGCAGAGATATTCTTCCTCATTTCGGCGGCCATCCAATGGCTGCGGGGATGACGCTGAAAGCCGAAGATGT
GCCGGATTTGCGAAGCAGGCTGAATGAAATAGCGGACAATACGCTTACGGAAGAGGATTTTATTCCCGTACAGGAAGTCG
ACCTTGTCTGCGGCGTCGAAGACATTACAGTTGAAAGCATCGCAGAAATGAACATGCTGTCTCCTTTTGGCATGCTGAAC
CCGAAACCGCACGTTCTCGTAGAAAACGCAGTACTGGAAGATGTCCGCAAGATAGGGGCAAATAAAACTCATGTCAAAAT
GACGATCAGAAACGAGTCGTCCCAGCTTGATTGCGTCGGTTTTAATAAAGGCGAGCTTGAAGAAGGCATCGTACCGGGTT
CAAGAATTTCTATTGTCGGTGAGATGTCAATCAACGAATGGAACAATCGAAAAAAGCCGCAGCTTATGATAAAGGATGCC
GCTGTTTCTGAATGGCAGCTGTTTGATTTAAGGGGAAAACGGACGTGGGAAGACACAGTTTCCGTCCTTCCATCTGCTAA
ACGGGCCATTGTCTCTTTCAAAGAAGACTCAACAACATTACTTCAAACTGAAGACCTTCGCCGAGAAGTGCACGTCATCA
GTTCTAAAGATCAAGCAAAGGCGTTTGACCTCGATGGTGCATATATTGTATTACTGGATCCGCCGCCTTCGCTGGACATG
CTCGCACGTTTGCTGGAAGGCAAGGCGCCCGAGCGGATATATTTTATTTTTCTCAATCATGAGGACCACTTTTTATCAAC
TTTTCCCGCAAGAGACCACTTTAAATGGTATTATGCGTTCTTGCTGAAAAGAGGCGCTTTTGACGTGAAAAAACACGGTT
CAGAGCTTGCAAAACATAAAGGCTGGTCCGTTGAAACAATCAATTTCATGACAAAGGTGTTTTTTGATCTCGGTTTTGTT
AAAATAGAGAATGGTGTGCTGTCTGTTGTGAGCGGAGCAAAGAAACGCGACTTAACAGACTCACAAACATATCAGGCGAA
ACAGCAGCTGATGGAACTGGATCAAAAGCTGAATTATTCTTCTGCTGAAGAGCTGAAGGAATGGCTGAATAAGCTGATGA
AACAAGATTCAGAGGCTTATGAAAGTACGAGGAGGACATAA

Domains

Predicted by InterproScan.

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(566-769)

DHH family (DHH)

(82-226)

RecJ OB domain (RecJ_OB)

(453-559)

DHHA1 domain (DHHA1)

(315-437)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

99.618

100

0.996

Multiple sequence alignment