Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   MKY32_RS15425 Genome accession   NZ_CP150259
Coordinates   2918673..2921021 (-) Length   782 a.a.
NCBI ID   WP_003183965.1    Uniprot ID   A0A415JD79
Organism   Bacillus sp. FSL K6-0998     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2913673..2926021
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKY32_RS15405 (MKY32_15405) - 2913715..2915286 (+) 1572 WP_011198158.1 SH3 domain-containing protein -
  MKY32_RS15410 (MKY32_15410) dtd 2915314..2915757 (-) 444 WP_003183959.1 D-aminoacyl-tRNA deacylase -
  MKY32_RS15415 (MKY32_15415) - 2915771..2917972 (-) 2202 WP_003183962.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -
  MKY32_RS15420 (MKY32_15420) - 2918143..2918655 (-) 513 WP_003183964.1 adenine phosphoribosyltransferase -
  MKY32_RS15425 (MKY32_15425) recJ 2918673..2921021 (-) 2349 WP_003183965.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  MKY32_RS15430 (MKY32_15430) - 2921093..2921386 (-) 294 WP_003183967.1 lipopolysaccharide assembly protein LapA domain-containing protein -
  MKY32_RS15435 (MKY32_15435) secDF 2921540..2923765 (-) 2226 WP_003183969.1 protein translocase subunit SecDF -
  MKY32_RS15440 (MKY32_15440) - 2923802..2924098 (-) 297 WP_003183971.1 post-transcriptional regulator -
  MKY32_RS15445 (MKY32_15445) spoVB 2924216..2925769 (+) 1554 WP_009327771.1 stage V sporulation protein B -

Sequence

Protein


Download         Length: 782 a.a.        Molecular weight: 87704.34 Da        Isoelectric Point: 4.9512

>NTDB_id=968397 MKY32_RS15425 WP_003183965.1 2918673..2921021(-) (recJ) [Bacillus sp. FSL K6-0998]
MLASKMRWDVQTPDKEKVEQLAGRLGITPLAASLLVMRGYDTADKASSFLYGNDARFHDPYLLKDMEKAVRRIKQAISDQ
EKIMIYGDYDADGVTSTTVLLQMLKKCSAQADFYIPDRFKEGYGPNEAAFRGIKESGYSLIITVDTGIAGVHEAEVAKEL
GIDLIITDHHEPGPVLPDAYAIIHPKQPGCGYPFKELAGVGVAFKLAHALNGELPEDLLDLCAIGTIADLVPLHDENRQI
AKRGMEQFRKTKRPGLKALAKIAGAEIHNADEETIGFQIAPRLNAVGRIEQADPAVYLLNTEDEREAEELAEDINALNKE
RQKIVNQMTEEAVQMIEEEGLDQPVIVVARPGWNPGVVGIVASKLVERFYRPAIVLGVDEEKGIAKGSARSIKGFDLFQN
LSECRDILPHFGGHPMAAGMTLNLEDVSALRERINRQAEAVLTEADFIPVQDVDLCCRLEDISVDSIAEMNLLAPFGTGN
PKPFVLVSDVKIDDARKIGANKNHLKLVLADDGNKLDCIGFYKGDIEEGIVPGAKISIVGELSINEWNNRKKPQLMIKDA
AVNEWQLFDLRGKRSWEEKILKLDPAKRACICFREETKAKLAGTGLEEELTVISGPEDAERLDLESRYAVFIDIPQSLDI
FDHVINGKKLERIYLIFYQSDEHFFSTFPTREYFKWYYAFLLKRGSFDLPKYGGELARHKGWTKDTIDFMTKVFFELGFV
TIENGVLSVVYHAEKRDLTDSETYREKQRLIELEQKLIYSSADELKQWLDKRMAEKAGAYKV

Nucleotide


Download         Length: 2349 bp        

>NTDB_id=968397 MKY32_RS15425 WP_003183965.1 2918673..2921021(-) (recJ) [Bacillus sp. FSL K6-0998]
ATGTTAGCGTCAAAAATGCGTTGGGACGTGCAAACGCCCGACAAAGAAAAGGTGGAGCAGCTCGCCGGAAGGTTAGGCAT
TACTCCCCTTGCCGCCTCATTGCTTGTCATGAGAGGTTACGATACAGCCGACAAAGCATCTTCATTTTTATATGGCAATG
ATGCACGTTTTCACGATCCTTATCTGCTGAAAGATATGGAAAAAGCTGTACGGCGAATCAAACAGGCAATTTCAGATCAA
GAAAAGATTATGATTTACGGCGATTATGATGCGGACGGTGTGACAAGCACGACTGTCCTTTTGCAAATGTTGAAAAAATG
TTCAGCACAAGCCGATTTTTATATACCTGACCGCTTTAAAGAAGGGTACGGTCCGAATGAAGCGGCCTTTCGCGGGATCA
AAGAATCAGGCTATTCATTGATCATTACAGTTGACACAGGCATTGCCGGCGTTCATGAAGCTGAAGTCGCTAAAGAGCTC
GGCATCGACCTGATCATTACGGATCACCATGAGCCGGGTCCCGTTTTGCCGGATGCTTATGCGATCATTCATCCGAAACA
GCCGGGCTGCGGCTATCCATTTAAAGAGCTTGCCGGCGTCGGTGTGGCATTTAAGCTTGCCCATGCCTTGAACGGAGAGC
TTCCGGAAGATTTGCTCGACCTTTGTGCGATCGGTACGATTGCCGATCTTGTGCCGCTTCATGATGAAAACAGGCAGATA
GCCAAGCGCGGCATGGAACAGTTCAGGAAAACGAAGCGTCCAGGATTAAAAGCGCTGGCGAAAATTGCCGGAGCGGAGAT
ACATAACGCGGATGAAGAGACCATCGGTTTTCAAATCGCGCCCCGCTTAAACGCGGTCGGCAGAATTGAACAGGCTGACC
CGGCCGTTTACCTCCTGAACACAGAAGACGAAAGAGAAGCGGAAGAGCTTGCTGAGGACATCAACGCCTTGAACAAGGAG
CGCCAAAAAATCGTCAATCAGATGACAGAGGAAGCCGTTCAGATGATCGAGGAGGAAGGCCTTGACCAGCCTGTGATCGT
CGTAGCGCGGCCGGGCTGGAATCCTGGCGTCGTGGGAATCGTGGCTTCAAAGCTTGTCGAGCGGTTTTACAGGCCCGCCA
TCGTCCTTGGCGTCGATGAAGAAAAAGGAATTGCCAAAGGATCTGCGCGCAGCATCAAAGGATTTGATTTATTTCAAAAC
CTTTCCGAGTGCCGGGATATTCTCCCGCACTTCGGCGGCCACCCGATGGCAGCCGGAATGACGCTCAACCTGGAAGACGT
TTCGGCTCTGAGGGAGAGAATCAACCGTCAGGCTGAAGCGGTTCTGACCGAGGCAGATTTTATCCCCGTCCAAGACGTCG
ATTTGTGCTGCCGGCTCGAAGATATATCGGTTGACAGCATTGCCGAAATGAACTTGCTGGCGCCGTTCGGCACGGGAAAC
CCGAAGCCGTTCGTTCTTGTCAGCGACGTAAAAATTGATGACGCCAGAAAAATCGGCGCCAACAAGAATCATTTGAAGCT
TGTGCTTGCAGACGACGGGAACAAGCTCGATTGCATCGGATTTTATAAAGGCGATATCGAAGAAGGAATCGTTCCTGGAG
CAAAGATCTCGATTGTCGGGGAACTGTCGATTAACGAATGGAATAACAGAAAAAAACCGCAGCTGATGATCAAAGATGCG
GCCGTCAATGAATGGCAGCTGTTTGATCTGCGGGGAAAACGGTCATGGGAAGAAAAAATATTGAAGCTGGACCCTGCGAA
ACGGGCGTGCATCTGCTTCCGCGAGGAAACAAAAGCAAAGCTGGCAGGGACAGGCCTTGAAGAAGAGCTGACCGTCATCA
GCGGCCCTGAAGATGCAGAGCGGCTCGACCTTGAGAGCCGTTATGCGGTATTTATAGATATTCCGCAGTCGCTCGACATC
TTTGATCATGTAATAAACGGCAAAAAGCTGGAGCGCATTTATCTGATTTTTTATCAATCAGACGAACATTTTTTCTCAAC
ATTCCCAACAAGGGAATATTTTAAATGGTACTATGCTTTCCTCCTGAAAAGAGGTTCTTTTGACTTGCCGAAATACGGCG
GAGAGCTTGCCAGACATAAAGGCTGGACAAAAGACACAATTGATTTTATGACAAAGGTGTTTTTTGAGCTCGGTTTTGTT
ACAATAGAGAATGGTGTTTTATCTGTTGTTTATCATGCCGAAAAGCGCGATTTAACAGATTCTGAAACCTACCGGGAAAA
ACAGCGCCTGATTGAATTGGAGCAAAAGCTCATTTATTCTTCGGCGGATGAGCTTAAACAGTGGCTGGATAAGCGGATGG
CGGAAAAAGCCGGCGCTTATAAAGTATGA

Domains

Predicted by InterproScan.

DHHA1 domain (DHHA1)

(315-436)

DHH family (DHH)

(82-226)

RecJ OB domain (RecJ_OB)

(453-559)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(566-769)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A415JD79

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

68.63

99.872

0.685

Multiple sequence alignment