Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   MKY32_RS05895 Genome accession   NZ_CP150259
Coordinates   1174331..1177831 (+) Length   1166 a.a.
NCBI ID   WP_254001813.1    Uniprot ID   -
Organism   Bacillus sp. FSL K6-0998     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1169331..1182831
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKY32_RS05870 (MKY32_05870) - 1169990..1171357 (-) 1368 WP_017474228.1 HAMP domain-containing sensor histidine kinase -
  MKY32_RS05875 (MKY32_05875) - 1171354..1172028 (-) 675 WP_009328903.1 response regulator transcription factor -
  MKY32_RS05880 (MKY32_05880) - 1172118..1172558 (-) 441 WP_009328902.1 ferritin-like domain-containing protein -
  MKY32_RS05885 (MKY32_05885) modA 1172762..1173556 (+) 795 WP_003180424.1 molybdate ABC transporter substrate-binding protein -
  MKY32_RS05890 (MKY32_05890) modB 1173565..1174221 (+) 657 WP_003180426.1 molybdate ABC transporter permease subunit -
  MKY32_RS05895 (MKY32_05895) addB 1174331..1177831 (+) 3501 WP_254001813.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  MKY32_RS05900 (MKY32_05900) addA 1177818..1181510 (+) 3693 WP_009328898.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  MKY32_RS05905 (MKY32_05905) sbcD 1181575..1182756 (+) 1182 WP_009328897.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1166 a.a.        Molecular weight: 134421.43 Da        Isoelectric Point: 5.5178

>NTDB_id=968362 MKY32_RS05895 WP_254001813.1 1174331..1177831(+) (addB) [Bacillus sp. FSL K6-0998]
MGAVFLSGRSGSGKTTFILNEIREKLRDEPLGKPIIFLVPDQMTFLMEYELSKTPDLGGTIRAQVYSFSRLAWRVLQHTG
GMNRPFLTGTGIQMLLRRLIEEHKGEFKVYQNASDKSGFTEQVERMLTEFKRHCLQPESIRDMIEGTGKSEYEDERILSD
KLHDLYILYSKLEENLENQYVQSEDYLTLLAEQIPYADEIRNAAVYVDGFHQFTPQEMNVLEQLMVHAEEITFSLTADKP
FTANSPNDLHLFRMTGKAYYDLYQKAKELGLDPSEVRLEETKRHRHHPELQHLERYFDERPAKPYPGQTESLRIMQASNR
RTEIEGIAREIHSLIRQGRFRLRDIAVIARNVEDYKDTIKEVFKDCELPFFIDGKESMQNHPLIELIRSTLDIIKGNWRY
EAVFRCVKTELLFPEGQPKERLREQIDQLENYCIAYGIKGDRWTSKDPFVYRRYASLDEDFAKTDKEIETENMLNELKGW
IVPPIHRLQKRLKKAETVREMAEAVYLYLEEADVPMKLEQERRLAEESGRIAESRQHEQVWDAVIQLLDEFVEMMGTERI
SFALFQQMIETGLESLKFALIPPALDQVFIGNMDLSRMYGTKCTFLIGVNDGILPARPADDGVLSDDDREWLKRNGAQLA
ATGREQLLDENFLIYMTLSSPSEKLYVSYPIADSEGKTLLPSTVVKRLNELFPDGEEKMLIHEPEQLDDEAQLEFLVNKG
IALSHLAGQLGIWTRQYAISDVWWSTYNFLMNEPDRIFSQNILSSLFFRNKVENLNRHVSRDLYGEHIQGSVSRMETFKA
CPFSHFASHGLKLKERQFFKLEAPDIGQLFHSALKLISDRLHELKLDWRDLTKAQCETLSSDAVERLAPKLQKEILLSSN
RHHYVKQKLQKIIARVSGILSEHAKASGFAPVGIELGFGGKGPLPPMRFTLKNGCTMELVGRIDRVDKAESSKGLLLRIV
DYKSSDKGLDLAEVYYGLALQMLTYLDLSITHSTDWLGMKASPAGVLYFHVHDPMIQASVPLGLDEIEKEIFKKFKMKGL
LLGDQEAVKLMDTTLEQGRSNIISAGLKKDGSLRSDSDVVAEEDFHVLRRHIRRTFQQAGEEITDGKVSIEPYKLKDRTP
CTYCSYRSFCQFDESLEENEYRILKPEKDSVILERLKKEDEADGDF

Nucleotide


Download         Length: 3501 bp        

>NTDB_id=968362 MKY32_RS05895 WP_254001813.1 1174331..1177831(+) (addB) [Bacillus sp. FSL K6-0998]
GTGGGAGCAGTATTTTTATCAGGCAGATCCGGGAGTGGGAAAACGACCTTTATCCTGAATGAAATCAGAGAAAAACTTCG
CGATGAACCGCTCGGAAAACCGATCATTTTTCTAGTCCCGGATCAAATGACGTTTTTAATGGAATACGAGCTTAGCAAAA
CACCCGATTTGGGCGGAACTATCCGCGCCCAAGTGTACAGTTTTTCAAGGCTTGCCTGGCGAGTGCTCCAGCATACCGGA
GGCATGAATCGGCCGTTTTTGACGGGGACGGGCATTCAGATGCTGCTGCGGAGACTGATTGAAGAGCATAAGGGCGAGTT
TAAAGTCTATCAAAACGCAAGCGATAAAAGCGGATTTACGGAGCAGGTGGAACGGATGCTGACCGAATTTAAACGGCATT
GTCTGCAGCCTGAAAGCATCCGGGACATGATAGAGGGGACGGGCAAATCGGAATACGAAGATGAACGGATCCTTTCCGAT
AAATTGCACGACTTATATATTTTATACAGCAAGCTTGAGGAAAATCTGGAGAATCAATATGTTCAATCTGAGGATTATTT
GACGCTTTTGGCCGAGCAGATTCCGTATGCGGATGAGATTAGAAACGCTGCCGTTTACGTGGACGGATTTCACCAATTTA
CGCCACAGGAGATGAACGTGCTGGAACAGCTGATGGTACATGCCGAAGAGATCACGTTTTCGCTGACGGCGGATAAGCCG
TTTACGGCAAACAGCCCGAATGATCTCCATCTGTTCAGGATGACCGGGAAGGCGTACTATGACCTTTACCAAAAGGCGAA
GGAACTCGGCCTTGATCCGTCTGAAGTGCGGCTGGAAGAAACGAAACGGCACCGGCATCATCCGGAGCTTCAGCATCTTG
AACGCTATTTTGATGAACGTCCTGCAAAACCGTATCCCGGACAGACGGAATCGCTCCGCATCATGCAGGCGTCGAACAGA
AGAACCGAGATTGAAGGAATTGCGCGCGAGATTCATTCTTTAATCCGCCAGGGCCGGTTCCGGCTTAGGGATATTGCTGT
CATCGCCCGCAATGTTGAAGATTATAAAGATACGATAAAAGAAGTTTTTAAAGACTGCGAACTTCCTTTTTTCATAGACG
GCAAGGAATCGATGCAAAATCATCCGCTCATTGAGCTGATCCGTTCGACGCTTGATATCATTAAAGGAAACTGGCGGTAT
GAAGCGGTGTTCCGCTGTGTCAAAACGGAACTTTTATTCCCGGAAGGACAGCCGAAGGAACGGCTGCGCGAACAGATTGA
CCAGCTTGAAAACTACTGTATTGCGTATGGCATTAAAGGAGACAGATGGACAAGCAAAGATCCGTTTGTTTACAGAAGGT
ACGCTTCACTGGATGAGGATTTTGCCAAGACAGACAAAGAGATTGAAACGGAAAATATGCTCAATGAACTAAAGGGCTGG
ATCGTTCCACCGATCCACAGGCTGCAGAAGCGCTTGAAAAAAGCTGAAACCGTCAGGGAAATGGCCGAAGCCGTTTACCT
TTATCTTGAAGAAGCCGATGTTCCGATGAAGCTTGAACAGGAAAGGCGGCTGGCCGAAGAAAGCGGTAGGATTGCAGAGT
CGCGCCAGCATGAGCAAGTATGGGATGCCGTTATTCAACTGTTGGACGAGTTTGTTGAAATGATGGGCACAGAGCGGATA
TCGTTTGCTTTATTTCAGCAAATGATTGAAACGGGCTTGGAGTCTTTGAAATTCGCGCTGATTCCGCCCGCTCTTGATCA
AGTGTTTATCGGCAATATGGACCTTTCAAGGATGTACGGCACGAAATGCACATTTCTCATCGGTGTAAACGACGGCATAC
TTCCGGCGAGGCCTGCGGACGACGGCGTGCTTTCAGACGATGACCGGGAGTGGCTGAAACGAAACGGCGCACAGCTGGCG
GCGACGGGTCGAGAGCAGCTCTTGGATGAGAATTTTCTGATCTACATGACGCTTTCCAGCCCGTCTGAAAAGCTCTATGT
CTCCTATCCGATCGCAGATTCGGAAGGGAAGACGCTGCTTCCTTCAACAGTGGTGAAACGGCTGAATGAGCTGTTCCCGG
ACGGCGAAGAGAAGATGCTGATTCATGAACCCGAGCAGCTGGACGATGAAGCCCAGCTTGAATTTCTCGTGAATAAAGGC
ATCGCCTTGAGCCATTTGGCAGGGCAGCTCGGCATCTGGACGAGGCAGTATGCGATCAGCGATGTATGGTGGAGTACATA
CAACTTTTTAATGAATGAGCCTGACCGCATCTTTTCTCAAAACATCCTGTCCAGCCTGTTTTTCAGAAACAAGGTCGAAA
ACCTAAACCGGCATGTGAGCCGCGATTTATACGGGGAGCACATTCAGGGAAGCGTGTCGCGGATGGAGACGTTCAAGGCC
TGTCCGTTTTCCCATTTTGCTTCGCACGGCTTAAAGCTGAAGGAACGCCAATTTTTCAAGCTTGAAGCTCCGGATATCGG
TCAGCTGTTCCACTCTGCGCTGAAGCTGATTTCAGACCGGCTTCACGAGCTTAAACTGGATTGGCGCGACTTAACGAAAG
CCCAATGCGAGACTTTGTCAAGCGATGCAGTTGAACGGCTCGCGCCAAAGCTGCAAAAAGAGATTTTGCTCAGTTCGAAC
CGTCATCATTATGTGAAGCAGAAGCTGCAAAAAATCATTGCCAGGGTATCGGGCATTTTAAGTGAGCATGCAAAAGCAAG
CGGCTTTGCCCCCGTCGGCATAGAGCTCGGATTCGGAGGGAAAGGTCCGCTTCCTCCAATGCGTTTCACCTTGAAGAACG
GCTGCACGATGGAGCTTGTCGGCCGGATCGACAGAGTGGATAAAGCGGAGAGCTCAAAAGGGCTGCTGCTTAGGATTGTT
GATTATAAATCAAGCGATAAAGGCCTCGATTTGGCCGAGGTGTATTACGGGCTTGCCCTGCAAATGCTGACATATCTCGA
TTTGTCGATCACGCATTCAACCGATTGGCTCGGCATGAAAGCATCCCCGGCAGGCGTGCTCTATTTTCATGTGCACGATC
CAATGATCCAGGCTTCAGTGCCGCTCGGCCTTGATGAAATAGAAAAAGAGATTTTCAAAAAGTTCAAGATGAAAGGGCTG
CTCCTGGGAGACCAGGAAGCGGTCAAGCTGATGGACACCACCCTGGAACAAGGCAGGTCGAATATTATCAGCGCCGGTTT
GAAAAAAGACGGCTCGCTCCGTTCGGATTCTGACGTTGTTGCAGAAGAGGATTTTCATGTATTGAGGCGTCATATCCGCC
GGACATTTCAGCAGGCCGGCGAGGAGATCACAGACGGCAAAGTGTCGATCGAGCCTTACAAGCTAAAAGACCGGACACCG
TGCACATATTGTTCATACCGGTCATTCTGCCAGTTTGACGAATCGCTTGAGGAAAACGAATACCGAATATTAAAACCTGA
AAAAGACAGCGTGATATTGGAGCGGCTGAAAAAGGAGGATGAGGCAGATGGAGATTTCTAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

74.163

99.914

0.741

Multiple sequence alignment